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Tissues

The facing pages show photomicrograph collages of the four principal tissue categories — epithelial

tissue, connective and supporting tissue, muscle tissue, and nervous tissue. The photomicrographs

 

illustrate the key structural features shared by the tissues in each category. Note the numerous

closely packed cells of the epithelial tissues and contrast them with the scattered cells and the fi brous surrounding matrix of the connective and supporting tissues. In the muscle tissue observe the long, slender specialized cells that are designed to shorten, and in the nerve tissue the branched, wire-like cells. We will explore each of the principal tissue categories in more detail on the pages that follow.

Epithelial Tissues

Connective and Supporting Tissues

4

Muscle Tissues

Nerve Tissues

5

Epithelial Tissue

Epithelial tissues are surface tissues that consist of numerous cells, with each cell forming membrane to membrane contact with its neighbors. As a general rule, descriptions of epithelial tissues are based on the shape of

their cells and on the number of cell layers present. By combining the shape names — squamous (fl at cells), cuboidal, and columnar — with the term simple if there is a single layer of cells or the term stratifi ed if there is more than one layer of cells, almost all of the epithelial tissues can be described and named. The photomicrographs on this page and the facing page represent the simple (single cell layer) epithelial tissues.

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1

Nucleus

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Mucous in goblet cell

 

2

Cytoplasm

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Microvilli

 

3

Cell membrane

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Basement membrane

 

4

Capillary lumen

10

Blood vessel with red blood cells

 

5

Glandular lumen

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Cilia

Simple squamous epithelium, mesothelium

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Connective tissue

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Basal cell

Section of mesentery, 400x

 

 

 

 

 

 

 

 

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Simple squamous epithelium, mesothelium

 

Simple squamous epithelium, endothelium

Surface view of mesentery, 400x

 

 

Section of capillary, 630x

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1

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3

Simple cuboidal epithelium

Urinary tubes in kidney - transverse section, 630x (left); longitudinal section, 400x (right)

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1

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Simple columnar epithelium

Section of mucosa of small intestine, 630x

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1

 

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Pseudostratified columnar epithelium

Section of mucosa of larynx, 400x

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Epithelial Tissue The photomicrographs on this and the facing page illustrate the stratifi ed (more than one layer of cells) epithelial tissues. Note that the tissues range

from two layers to numerous layers and the cell shape used for the tissue name is the shape of the cells found in the surface layer.

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1

1

Nucleus

 

 

 

2

Cytoplasm

 

 

3

3

Basal cell layer

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4

Intermediate cell layer

 

 

 

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Superficial cell layer

 

 

 

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Stratum basale

Nonkeratinized stratified squamous epithelium

 

 

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Stratum spinosum

 

 

Section of esophageal mucosa, 200x

 

 

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Stratum granulosum

 

 

 

 

 

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Stratum lucidum

 

 

 

10Stratum corneum

11Connective tissue

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Basement membrane

 

 

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Glandular lumen

 

 

 

 

 

 

 

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1

 

 

 

 

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Keratinized stratified squamous epithelium

Section of skin, 200x

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3

1

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1

Stratified cuboidal epithelium

Section of duct of esophageal gland, 400x

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1

2

1

1

 

 

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Stratified columnar epithelium

Transitional epithelium

Section of pharyngeal mucosa, 400x

Section of urinary bladder mucosa, 400x

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Connective Tissue

Connective tissues have relatively few cells and the cells are surrounded by an extracellular matrix of fi bers, which the cells secrete. The classifi cation and names of connective tissues arise from the type

and arrangement of the fi bers produced by the cells and secreted into the surrounding matrix. There are three named

bers in the matrix — collagen fi bers, reticular fi bers (actually a thin form of collagen), and elastic fi bers. The fi bers are deposited in varying degrees of density and are arranged in different patterns. The tissue names are based on the different

ber types and patterns in the matrix.

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Mast cell

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Cytoplasm

2

Fibroblast

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Plasma membrane

3

Collagen fiber

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Lipid storage area

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Elastic fiber

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Nucleus of reticular cell

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Reticular fiber

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Nucleus of fibroblast

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Nucleus of adipose cell

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Elastic lamella

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2

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2

3

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1

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1

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2

Loose (areolar) connective tissue

Section of subcutaneous layer of integument, 400x

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Adipose tissue

Section of epicardial fat, 200x

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Reticular tissue

 

 

Section of lymph node, 400x

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Dense irregular connective tissue

Dense regular (collagenous) connective tissue

Section of dermis, 200x

Section of tendon, 200x

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Dense regular (elastic) connective tissue

Section of tunica media of aorta, 400x

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Supporting Tissue

The supporting tissue category consists of the skeletal tissues— cartilage and bone. Like the connective tissues, the supporting tissues have relatively few cells surrounded by a signifi cant amount of extracellular

matrix, which for the most part the cells produce. However, unlike the soft matrix of the connective tissues, the extracellular matrix of the supporting tissues is firm and rubber-like in cartilage and hard in bone tissue.

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Hyaline ground substance

 

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Collagen fibers in ground substance

 

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Elastic fibers in ground substance

 

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Chondrocyte nucleus

 

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Chondrocyte in lacuna

 

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Perichondrium

 

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Bone trabecula

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Osteocyte

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Red bone marrow

 

10Canaliculi

11Lacuna

12Lamella

13Central canal

1

Hyaline cartilage

Section of cartilage in developing fetal bone, 200x

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2

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2

Fibrocartilage

Elastic cartilage

Section of intervertebral disc, 200x

Section of cartilage from auricle of ear, 400x

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Spongy bone

Section of epiphysis of metacarpal bone, 200x

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Compact bone

Section of diaphysis of fibula, 100x; callout of osteon, 400x

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Hematolymphoid Complex

The tissues blood and lymph traditionally were classified as connective tissues because, like all connective tissues, the extracellular matrix

is a greater percentage of the tissue then are the cells. However, the extracellular matrix of blood and lymph is a liquid matrix called plasma, rather than the soft, fi rm matrix of connective tissues. The most recent Terminologia Histologica places blood and lymph in their own subcategory called the hematolymphoid complex.

 

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Erythrocyte or red blood cell (rbc)

 

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Leukocyte or white blood cell (wbc) - neutrophil

 

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Leukocyte or white blood cell (wbc) - monocyte

 

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Thrombocyte (platelet)

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Plasma

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Crenated red blood cell

 

 

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Blood smear

Wright’s stain, 200x; enlargement, 630x; individual cells, 1500x

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Muscle Tissue

Muscle cells are long, slender cells that have special arrangements of the proteins actin and myosin within the cytoplasm. The architectural design of these proteins forms the muscle cell “machinery” that allows the cell to specialize at contracting

(shortening). The names of the different types of muscle tissues arise from the arrangement of the contractile proteins within their cells. In some tissues the protein arrangement gives the cell a striated, or striped, appearance (striated muscle), while in other tissues the striped appearance is not evident (non-striated or smooth muscle).

1 Nucleus

2 Sarcoplasm

3 Smooth muscle cell

4 Cardiac muscle cell

5 Skeletal muscle cell

6 Intercalated disc

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3

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Smooth (nonstriated) muscle tissue

Longitudinal section of muscular wall of intestine, 500x

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Cardiac striated muscle tissue

Skeletal striated muscle tissue

Section of ventricle of heart, 500x

Section of vastus lateralis muscle, 400x

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