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Файл:Ординатура / Хирургия / Библиотека им академика М.И. Перельмана / Книга_5217_Библиотеки_им_академика_М_И_Перельмана.pdf
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- •Also of interest
- •Contents
- •Part I: Introduction
- •1.1.2.3 Sustainability and future perspectives
- •1.2 Alkaloids, flavonoids, terpenoids, and other active compounds
- •1.2.1 Alkaloids
- •1.2.2 Flavonoids
- •1.2.3 Terpenoids
- •1.2.4 Other active compounds
- •1.3 Chemical structures and pharmacological effects
- •1.3.1 Chemical structures and effects of alkaloids
- •1.3.2 Chemical structures and effects of flavonoids
- •1.3.3 Chemical structures and effects of terpenoids
- •1.3.4 Structures and effects of other compounds
- •1.4.2 Flavonoids
- •1.4.3 Terpenoids
- •1.4.4 Other active compounds
- •1.5 Chemical structures and pharmacological effects
- •1.5.1 Chemical structures and effects of alkaloids
- •1.1 Introduction to medicinal and aromatic plants
- •1.1.1 Historical background
- •1.1.1.1 Historical background
- •1.1.2 Traditional and modern uses
- •1.1.2.1 Traditional uses
- •1.1.2.2 Modern uses
- •1.5.2 Chemical structures and effects of flavonoids
- •1.5.3 Chemical structures and effects of terpenoids
- •1.5.4 Structures and effects of other compounds
- •1.6 Aromatic plants in everyday life
- •1.6.1 The importance of essential oils and aromatherapy
- •1.6.2 Applications in the cosmetics and food industry
- •1.6.3 Food industry
- •1.7.1 Protection of endangered species
- •1.7.2 Sustainable harvesting methods
- •1.8.1 Protection of endangered species
- •1.8.1.1 Threats to endangered species
- •1.8.2 Conservation strategies
- •1.8.2.1 Protection of natural habitats (in situ conservation)
- •1.8.3 Participation of local communities
- •1.8.3.1 Education and awareness
- •1.8.3.2 International collaborations
- •1.8.3.3 Sustainable harvesting and trade
- •1.8.4 Sustainable harvesting methods
- •1.8.4.1 The importance of sustainable harvesting
- •1.8.4.2 Sustainable harvesting principles
- •1.8.4.3 Sustainable harvesting techniques
- •1.8.4.4 Monitoring and evaluating the harvesting process
- •1.8.4.5 The economic dimension of sustainable harvesting
- •1.8.4.6 International approaches and legal regulations
- •1.8.4.6.1 International approaches
- •1.8.4.6.2 Legal regulations
- •1.8.4.6.3 Protection of local communities and traditional knowledge
- •1.8.5 Many countries are protecting biodiversity
- •1.8.5.1 Global conservation efforts
- •1.8.5.2 Protected areas and conservation in natural habitats
- •1.8.5.3 Ex situ conservation and gene banks
- •1.9 Challenges and future prospects
- •1.9.1 Impacts of climate change
- •1.9.2 Genetic and biotechnological approaches
- •1.9.2.1 Protection of genetic diversity and breeding studies
- •1.9.2.2 Genomic and transcriptomic approaches
- •1.9.2.3 Culture tissue techniques
- •1.9.2.4 CRISPR/Cas9 technology
- •1.9.2.5 Metabolic engineering and synthetic biology
- •1.9.2.6 Bioinformatics and data analysis
- •1.10 Case studies and regional practices
- •1.10.1 Successful projects in specific regions
- •1.10.1.1 India: Ayurveda and biodiversity conservation projects
- •1.10.1.2 Brazil: sustainable collection projects in the Amazon forest
- •1.10.1.3 Turkey: protection and production of endemic plants
- •1.10.1.4 Africa: integration of local knowledge with modern practices
- •1.10.2.1 Documentation and protection of traditional knowledge
- •1.10.2.2 Scientific validation and application
- •1.10.2.3 Education and awareness
- •1.10.2.4 Patents and intellectual property rights
- •1.10.2.5 Public and private sector collaboration
- •1.11 Conclusions
- •References
- •2.1 Introduction
- •2.3.1 Plant selection
- •2.3.1.1 Random plant selection
- •2.3.1.2 Plant selection based on ethnopharmacology and traditional uses
- •2.3.1.3 Plant selection by HTS technologies
- •2.3.1.4 Plant selection through virtual screening
- •2.3.1.5 Phytochemical databases
- •2.3.2.1 Comminution and homogenization
- •2.3.3 Extraction
- •2.3.3.1 Conventional extraction techniques
- •2.3.3.2 Maceration
- •2.3.3.3 Infusion
- •2.3.3.4 Decoction
- •2.3.3.5 Percolation
- •2.3.3.13 Pressurized liquid extraction
- •2.3.3.14 Enzyme-assisted extraction
- •2.3.3.15 Solid-phase microextraction
- •2.3.3.6 Hydrodistillation and steam distillation
- •2.3.3.7 Soxhlet extraction
- •2.3.3.8 Advanced extraction techniques
- •2.3.3.9 Ultrasound-assisted extraction
- •2.3.3.10 Pulsed-electric field extraction
- •2.3.3.11 Microwave-assisted extraction
- •2.3.3.12 Supercritical extraction
- •2.3.3.16 Bioassay-guided fractionation of plant extracts
- •2.3.4 Isolation and purification
- •2.3.4.3 Gas chromatography (GC)
- •2.3.4.4 Column chromatography (CC)
- •2.3.4.5 Ion exchange chromatography (IEC)
- •2.3.5 Elucidation of the chemical structure
- •2.3.5.1 Nuclear magnetic resonance (NMR)
- •2.3.5.2 Mass spectrometry (MS) and high-resolution mass spectrometry (HRMS)
- •2.3.5.4 UV-visible spectroscopy
- •2.3.6 Evaluation of therapeutic efficacy with bioassays
- •2.3.7 Preclinical and clinical researches
- •2.3.8 Structural modifications and developing new analogues
- •2.4 The use of omics technologies in drug discovery and development
- •2.4.1 Genomics
- •2.4.2 Metabolomics
- •2.4.3 Proteomics
- •2.5 Future scope
- •2.6 Conclusion
- •References
- •3.1 Introduction
- •3.2 Bioactive compounds
- •3.2.1 Alkaloids
- •3.2.2 Terpenoids (terpenes)
- •3.2.3 Phenolics
- •3.3 Industrial importance of biological active compounds
- •3.4 Industrial use of MAPs
- •3.5 Essential oils
- •3.6 MAPs in the dye industry
- •3.6.1 Use of MAPs in the perfumery
- •3.6.2 Use of MAPs in cosmetics
- •3.6.3 Use of MAPs in plastic production
- •3.6.4 Other industrial applications
- •3.6.5 MAPs in energy production
- •3.6.6 MAPs in agricultural applications
- •3.7 Salt stress
- •3.7.1 Nutrient
- •3.7.2 Productivity
- •3.7.3 Photosynthesis
- •3.8 Drought stress
- •3.9 Heavy metals
- •3.10 Heat stress
- •3.11 Soil pH
- •3.12 Light intensity
- •3.13 Pest and disease management
- •3.14 Conclusion and future perspective
- •References
- •4.1 Introduction
- •4.2 Toxic compounds and their effects
- •4.2.1 Alkaloids
- •4.2.2 Glycosides
- •4.2.3 Essential oils
- •4.2.4 Saponins
- •4.2.5 Coumarins
- •4.3 Poisonous medicinal plants
- •4.3.1 Digitalis purpurea (foxglove)
- •4.3.2 Atropa belladonna (deadly nightshade)
- •4.3.3 Aconitum napellus (monkshood, aconite)
- •4.3.4 Conium maculatum (hemlock)
- •4.3.5 Nerium oleander (oleander)
- •4.3.6 Datura stramonium (jimsonweed)
- •4.3.7 Ricinus communis (castor bean)
- •4.3.8 Taxus baccata (English yew)
- •4.3.9 Hyoscyamus niger (black henbane)
- •4.3.10 Cicuta virosa (water hemlock)
- •4.3.11 Veratrum viride (false hellebore)
- •4.3.12 Helleborus niger (Christmas rose)
- •4.3.13 Mandragora officinarum (mandrake)
- •4.3.14 Ageratina altissima (white snakeroot)
- •4.3.15 Bryonia alba (white bryony)
- •4.3.16 Colchicum autumnale (autumn crocus)
- •4.3.17 Chelidonium majus Linn. – Papaveraceae
- •4.4 Aromatic plants and poisons
- •4.4.1 Artemisia absinthium (wormwood)
- •4.4.2 Sassafras albidum (sassafras)
- •4.4.3 Lavandula angustifolia (lavender)
- •4.4.4 Rosmarinus officinalis (rosemary)
- •4.4.5 Mentha pulegium (pennyroyal)
- •4.4.6 Eucalyptus globulus (eucalyptus)
- •4.4.7 Myristica fragrans (nutmeg)
- •4.4.8 Thuja occidentalis (white cedar)
- •4.4.9 Illicium verum (star anise)
- •4.4.10 Syzygium aromaticum (clove)
- •4.4.11 Juniperus sabina (savin juniper)
- •4.4.12 Pimpinella anisum (anise)
- •4.4.13 Lavandula stoechas (French lavender)
- •4.4.14 Artemisia vulgaris (mugwort)
- •4.4.15 Melaleuca alternifolia (tea tree)
- •4.4.16 Pelargonium graveolens (rose geranium)
- •4.5 Safe use and precautions
- •4.5.1 Safety guidelines and precautions
- •4.6 Conclusions
- •References
- •5.1 Introduction
- •5.2 Effect of drought or water deficiency on the morphology of medicinal plants
- •5.4 Effect of drought or water deficiency on secondary metabolites of medicinal plants
- •5.5 Different approaches to mitigate the negative effects of drought stress on plants
- •5.6 Case studies
- •5.7 Conclusions
- •References
- •6.1 Introduction
- •6.2 Importance of medicinal and aromatic plants
- •6.3 Salinity effect on medicinal plants
- •6.3.1 Effects on growth and development
- •6.3.2 Impact on photosynthesis and water relations
- •6.3.3 Ionic stress and nutrient imbalance
- •6.3.4 Oxidative stress and antioxidant response
- •6.3.5 Impact on secondary metabolite production
- •6.4 Molecular responses to salinity stress
- •6.5.1 Amino acids
- •6.5.2 Proteins
- •6.5.3 Carbohydrates
- •6.5.4 Lipids
- •6.6 Study of alkaloids through proteomic and other approaches
- •6.7 Phenolic compounds during stress
- •6.8 Strategies for improving salt tolerance in MAPs
- •6.8.1 Exogenous application of plant growth regulators
- •6.8.2 Use of beneficial microorganisms
- •6.8.3 Genetic approaches
- •6.8.4 CRISPR/Cas9 gene editing
- •6.8.5 Agronomic practices
- •6.8.6 Use of mulches
- •6.8.7 Application of organic amendments
- •6.8.8 Silicon supplementation
- •6.8.9 Application of polyamines
- •6.8.10 Nanofertilizers and nanoparticles
- •6.8.11 Application of melatonin
- •6.9.1 Water relations and osmotic adjustment
- •6.9.2 Ion homeostasis and nutrient balance
- •6.10 Molecular mechanisms of salt tolerance
- •6.11.1 Genetic engineering strategies
- •6.11.2 Identification of salt-tolerant genes
- •6.11.3 Use of plant growth regulators
- •6.12 Conclusion and key points
- •References
- •7.1 Introduction
- •7.2 Heavy metals and their effects on the environment
- •7.4 Processes of heavy metal uptake by roots
- •7.5 Transport and accumulation in various plant tissues
- •7.8 Plant defense mechanisms against heavy metals
- •7.9 Molecular and genetic responses to heavy metal contamination
- •7.11 Selection of heavy metal-resistant plants
- •7.12 Case studies
- •7.13 Conclusions
- •References
- •8.1 Introduction
- •8.2 Metabolic and hormonal responses to abiotic stress
- •8.3 Water stress
- •8.3.1 Drought stress
- •8.3.2 Waterlogging stress
- •8.4 Temperature stress
- •8.4.1 High temperature (heat shock)
- •8.4.2 Low-temperature stress
- •8.5 Light stress
- •8.6 Salt stress
- •8.7 Nutrient stress
- •8.8 Heavy metal stress
- •8.9 Molecular docking calculation for stress
- •8.10 Conclusion
- •References
- •Part III: Pharmaceutical use of medicinal plants
- •9.1 Introduction
- •9.2 General properties of medicinal and aromatic plants used in burn treatment
- •9.2.1 Phytochemical content and mechanisms of action
- •9.2.2 Antimicrobial effects
- •9.2.3 Wound-healing effects
- •9.2.4 Analgesic effects
- •9.2.5 Advantages and disadvantages of herbal treatments
- •9.2.5.1 Advantages
- •9.2.5.2 Disadvantages
- •9.3 Medicinal and aromatic plants used in burn treatment
- •9.3.1 Aloe vera
- •9.3.1.1 Clinical effects
- •9.3.2 Calendula officinalis (Calendula)
- •9.3.3 Centella asiatica (gotu kola)
- •9.4 Molecular basis of plant action mechanisms
- •9.4.1 Cellular mechanisms in wound healing
- •9.4.2 Innovative research methods in herbal treatments
- •9.4.2.1 Omic technologies: genomic, proteomic, and metabolomic approaches
- •9.5 Formulation and application methods of herbal products
- •9.5.1 Pharmaceutical formulations
- •9.5.2 Dosage and application methods
- •9.5.3 Nanotechnological approaches
- •9.5.4 Factors affecting chemical stability
- •9.5.4.1 Stability enhancement methods
- •9.5.4.2 Importance of storage conditions
- •9.5.4.3 Stability tests and quality control
- •9.6 Clinical research and evidence-based practices
- •9.6.1 Clinical studies
- •9.6.2.1 Meta-analyses and literature reviews
- •9.7 Safety and side effects
- •9.7.1 Toxicological risks
- •9.7.2 Side effects and contraindications
- •9.8 Integration of traditional knowledge and modern science
- •9.8.1 Ethnobotany and traditional knowledge
- •9.8.2 Cultural and regional diversity
- •9.9 Future research areas and innovation
- •9.9.1 Pharmacogenetics and personalized medicine
- •9.9.2 Biodegradable and smart materials
- •9.9.3 Combined use of herbal treatments
- •9.10 Conclusion
- •References
- •10.1 Introduction
- •10.2 COPD
- •10.3 Asthma
- •10.4 Pneumonia
- •10.5 Lung cancer
- •References
- •11.1 Introduction
- •11.2 Oxidative stress
- •11.2.1 Reactive oxygen species
- •11.2.2 Sources and generation of free radicals
- •11.3.1 Lipid peroxidation
- •11.3.2 Protein oxidation
- •11.3.3 DNA oxidation
- •11.4 Defense of the organism against ROS
- •11.4.1 Free radicals and antioxidants
- •11.4.2 Antioxidants action mechanism
- •11.5 Methods for determination of antioxidative activity
- •11.5.1 Methods based on hydrogen atom transfer
- •11.5.2 Methods based on electron transfer
- •11.5.3 Other methods for determination of antioxidant potential
- •11.6 Medicinal and aromatic plants as natural antioxidants
- •11.7 MAPs with antioxidant activity
- •11.8 Conclusion
- •References
- •12.1 Introduction
- •12.2 Definition, historical documents, and distribution related to the study of the usage of MAPs
- •12.3 Antibacterial activity of MAPs
- •12.4 Extracts and essential oils from MAPs as antibacterial agents
- •12.5 Compounds of essential oils with antibacterial properties and their activity against a variety of bacterial strains
- •12.6.2 Terpenoids from MAPs as antibacterial agents
- •12.6.3 Alkaloids from MAPs as antibacterial agents
- •12.7.2 Clinopodium nepeta (L). Kuntze
- •12.7.3 Lavandula officinalis
- •12.7.4 Helichrysum italicum
- •12.7.5 Mentha piperita
- •12.8 Conclusion
- •References
- •13.1 Introduction
- •13.2 Medicinal and aromatic plant-derived extracts
- •13.2.1 Extraction techniques of MAPs
- •13.2.2 Influence of extraction operational parameters
- •13.3 MAPs in skin care products
- •13.3.1 MAPs as photoprotective agents against UV light and skin damage
- •13.3.2 Regenerative and wound-healing properties of MAP-derived agents
- •13.3.3 MAPs as skin anti-aging and whitening agents
- •13.4 MAPs in hair cosmetics
- •13.4.1 MAPs in hair products
- •13.4.2 MAPs in hair growth products
- •13.5 MAPs in oral hygiene products
- •13.5.1 Formulations for toothpaste and mouthwash
- •13.5.2 MAPs in prevention of dental caries
- •13.6 MAPs enhanced by sustainable materials in cosmetics
- •13.6.1 Nanotechnology in cosmetic formulations
- •13.6.2 Innovative nanocarrier materials
- •13.7 Conclusion
- •Abbreviations
- •References
- •14.1 Introduction

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Part IV: Uses of medicinal and aromatic plants
in other areas


Emina Boškailo✶, Alema Dedić, Hurija Džudžević Čančar,
Amra Alispahić, and Kasapović Dejana
Chapter 13
Medicinal and aromatic plants used
in cosmetics
Abstract: In order to promote sustainability and environmentally friendly practices,
the chapter delves into medicinal and aromatic plant (MAP) extracts, their phytochemi
cal, and their integration with novel technology aimed to promote applicability in cosmetic formulations. Huge progress is being made in replacing harnessing solvent with
green substitutions, and increasing extraction process efficiency by manipulating pa
rameters. Consequently, MAP extracts have complexed chemical profile and significant
biological activities such as antioxidant, anti-inflammatory, antimicrobial, etc. MAPs are
accelerating the revolution as environmentally benign materials with prominent usage
in cosmetic industry. This chapter also emphasizes the significance of MAP extracts col
laboration with nanoparticles (1–100 nm) as creative solutions being developed to leverage delivery of active ingredients, causing site-specificity, enhancing biocompatibility,
or the drug-loading capacity. Therefore, the combination of MAP-derived nanolipo
somes, nanocarriers such as ultradeformable vesicles have significant impact in upgrading the skin penetration of drugs and efficacy of anti-ageing performances of some
metabolites. Tocoferol in transfersome (<100 nm), has great properties with entrapment
potentiality of up to 90%. Such potential gives formulations another dimension, repre
senting them as an eco-friendly source of materials for cosmetics.
-
-
-
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Keywords: medicinal and aromatic plants, biodiversity, extraction, sustainability,
nanotechnology, cosmetic products
✶
Corresponding author: Emina Boškailo, Department of Ecology and Environmental Protection,
Faculty of Social Sciences Dr. Milenko Brkić, Herzegovina University, Mostar 88000, Bosnia and
Herzegovina; International Society of Engineering Science and Technology, Nottingham, UK,
e-mail: emina.zubovic@gmail.com; emina.boskailo@hercegovina.edu.ba
Alema Dedić, Hurija Džudžević Čančar, Amra Alispahić, Department of Chemistry in Pharmacy,
University of Sarajevo-Faculty of Pharmacy, Sarajevo, Zmaja od Bosne 8 – Campus UNSA, Bosnia and
Herzegovina
Kasapović Dejana, Department of Physics and Chemistry, Faculty of Engineering and Natural Sciences,
University in Zenica, Zenica 72000, Bosnia and Herzegovina

460 Emina Boškailo et al.
13.1 Introduction
Since ancient times plants have served as primary care in protecting health. Nowadays, the prevalence of traditional use of plants is almost 80% in low-income countries. The world is rich in plant biodiversity that has broad of benefits for human
health, so researchers have gained huge interest in plant investigation (qualitative
and quantitative). There is increasing evidence for the significant value of medicinal
and aromatic plants (MAPs) around the globe. Their use in numerous industry areas
is influenced by their life-sustaining element content, role of oxygen provider, and
sources of myriad of compounds beneficial for functioning of organisms. As the
human population increases, intensity of wild plant use is high, but inappropriate ecosystem management and exploitations are a threat to medicinal plants [1]. Their medicinal potential has increased, especially in cosmetic industry for their functions on
prevention and treatment in skin care. MAPs utilization has improved approaches in
treatment of physical disorders, minimized synthetic antibiotics use, and prolonged
life expectancy. As per the last estimation, nearly 223,300 seed plants have been investigated or known to humans, but only one-fifth are pharmacologically and chemically
researched for novel drug discovery [2].
Antioxidant and antimicrobial potential of MAPs are high, due to polyphenols composition known as free radical scavengers, which prevent development of various diseases. The antioxidant activity they possess is responsible for their wide application in
cosmetic industry. When humans are exposed to stress, they make very reactive oxygenbased species (ROS and RNS), and less non-enzymatic (e.g., vitamin E, tocopherols) and
enzymatic antioxidants (e.g., superoxide dismutase). The pharmaceutical industry is dedicated to develop products based on MAPs, in particular based on their constituents, phenolics, terpenes, and alkaloids. Singh et al. [3] evaluated Ajuga integrifolia Buch.-Ham.
leaf extracts (methanol, hexane, and water) for their chemical profile as well as antioxidant and antibacterial activity. Methanol extract showed the highest content of phenolic
(196.16 ± 0.0083 mg GA equivalent/g) and flavonoid (222.77 ± 0.002 mg RU equivalent/g).
DPPH test was used to examine the antioxidant activity and showed a minimum IC50
value. The highest inhibition zone (IZ) and minimum inhibitory concentration (MIC) was
given in MRSA and β-lactam-resistant E. coli.
Unfortunately, concerns about synthetic products are growing due to their negative
impact on human health, so preference for the innovative MAP-based cosmetics has
peaked. Hence, in the European market, demand for MAP-based cosmetics has reached
up to $4 billion in 2015, and keeps growing annually up to 11%. Rosemary essential oils
and extracts are known for a plethora of biological potentials like antioxidant, antiinflammatory, wound-healing, anti-wrinkle properties etc. These activities are related to
various and versatile rosemary chemical contents (e.g., rosmarinic acid, carnosol, and
carnosic acid). Hexane extract of
based mucoadhesive gel (particle size of 56.55–66.13 nm) showed antiaging potential. Collagen, elastin, and hyaluronic acid are crucial for the dermal extracellular matrix (ECM),
Rosmarinus officinalis formulated in lipid nanocapsule-

Chapter 13 Medicinal and aromatic plants used in cosmetics 461
and Rosmarinus officinalis hexane extract showed anti-collagenase activity of IC50 of
520.2 µg/mL. Moreover, in vitro anti-elastase (IC50 value of 57.6 µg/mL), and antihyaluronidase activities (448.1 µg/mL) improved antiaging potential of extracts [4].
The concept of One Health is aligned with UN sustainable development goals (SDGs)
and a multidisciplinary approach including animal, environmental, and human health
makes social approach correlated and ensures positive growth of medicinal products for
human beings [5]. Before any cosmetic product gets on the market it needs to be proven
for safety by assessment. Animal testing of cosmetics products is forbidden, so numerous
alternatives such as 2D cell culture models or 3D human skin equivalent models are applied in order to investigate anti-inflammatory activity or skin irritation efficiency [6].
13.2 Medicinal and aromatic plant-derived extracts
13.2.1 Extraction techniques of MAPs
Natural products represent chemical constituents or phytochemicals isolated or produced from plants investigated for various purposes in numerous research fields. MAPs
are a huge source of phytochemical extremely important for pharmaceutical industry,
cosmetics, and nutraceuticals. MAP-based extracts like essential oils, concretes, resinoids,
etc. consist of numerous chemical compounds groups (e.g., terpenoids, alkaloids, flavonoids, phenolics, and alcohols). They contribute to sensorial (taste and flavor) and functional (antioxidant, anti-cancer, and antimicrobial activities) preferences. Extraction as a
simple method mainly implies selective solvent application (e.g., water, alcohol, and
their mixture in various ratios) to dissolve chemical compounds for their separation and
characterization. There is also a multitude of extraction techniques for production of
natural products. The following parameters determine what type of extraction will be
applied: the drug nature (quality of plant, its origin, climate conditions, harvest time,
plant’s organ, drying method, particle size, etc.), solvent type, costs and therapeutic values, concentration of the product, and stability of the drug (maceration, hydrodistillation, HD, Soxhlet extraction, SE). Such extraction approaches have pros and cons and
are rapid, but mainly require organic and costly solvents.
Considering these drawbacks, innovative approaches are desirable such as ultrasound- and microwave-assisted extraction (UAH and MAH), supercritical fluid extraction
(SFE), enzyme-assisted extraction (EAE), etc. Kırkıncı et al. [7] evaluated the effect of conventional and innovative extraction methods and revealed potential of HD and MAH on
essential oil (EO) and wastewater (WW) yield and chemical profile of Lavandula angusti-
folia L. Accordingly, the potential for utilizing WW in numerous industrial applications
is huge, for EOs and food, cosmetic, and health sectors. Both techniques were consistent
in extracting major compounds by GC/MS (α-terpinolene (25.60% and 24.25% and (–)borneol 19.55% and 19.37%), but the difference is visible through presence of minor com-

462 Emina Boškailo et al.
pounds. MAH extract has higher phenolic and flavonoid content versus HD probably
influenced by minimized thermal degradation and selective heating mechanisms. Moreover, MAH extract has shown better antioxidant activity. Naik et al. [8] used SE and SFE
to extract P. juliflora. Regarding GC/MS analysis, 35 components were eluted by SFE and
proved better antifungal activity. These studies highlight the importance of extraction
methods in recovering phytochemicals as potential natural metabolites that may provide
a positive impact on tested activities.
13.2.2 Influence of extraction operational parameters
Besides the impact of extraction techniques, other parameters might influence extracts:
yield, composition, and results for tested activities are given in Table 13.1. For example,
altitude influence (from 766 m to 1,387 m, with 100-m intervals) on O. majorana essential
oil content, composition, and yield was evaluated. The best EO yield (6.50%) and linalool
content (79.84%) were estimated by HD at the lowest altitudes (766 m). The highest yield
of borneol, caryophyllene, linalool oxide, germacreneand bicyclogermacrene compounds was achieved at 890 m altitude. Also, thymol and αterpineol yield, as well as a-terpinene, terpinene-4-ol, cis-sabinene hydrate, and carvacrol
were achieved at highest altitudes (1180 and 1,387 m). Climate parameters (temperature
and its differences during the day and night, relative humidity, precipitation, day light
hours, and intensity) change with altitude, and consequently affect EO yield and composi
tion [9].
SC−CO
extraction was used to achieve yield of chia seed oil by manipulating differ-
2
ent operational parameters (pressure, temperature, and particle size) and compared
with SE results. The optimal SC−CO2 parameters (335 bar, 45 °C, 100 − 400 μm, 24 s of
grinding time) have contributed to the highest yield versus chia oil yield obtained by SE
[10]. Puertolas et al. [11] investigated the impact of pulse electric field (PEF)-assisted extraction on olive seeds using the following parameters: electric field of 2 kV/cm and
65 J of energy. These conditions increased the yield up to 13.3%. Success of PEF is visible
through content increase of compounds that belong to polyphenols (11.5%), phytosterols
(9.9%) and total tocopherols (15%). Moreover, apple seed oil is obtained by SFE and SE
considering optimization pressure, temperature, and CO
1–8 L/h) on the yield, antioxidant activity, and total phenolic content. The best SFE parameters were 24 MPa, 40 °C, 1 L/h of CO
flow rate, 140 min that obtained the highest yield
2
20.5 ± 1.5% (w/w) compared to SE yield of 22.5 ± 2.5% (w/w). Moreover, SFE contributed to
higher linoleic acid content and better oxidative stability [12].
Vo et al. [13] optimized the UAE and MAE methods using a combination of three
solvents (ethanol, acetone, and water) with an optimum ratio of 0.29:0.34:0.37, that
achieved an appropriate polarity for recovering phenolics and flavonoids from passion
fruit peels. The optimal UAE conditions were 28 mL/g of liquid-to-solid ratio (LSR),
608 W of ultrasonic power, and 63 °C for 20 min to achieve TPC of 39.38 mg GAE/g db
D, trans-linalool oxide, a-humulene,
flow rate (10–30 MPa, 40–60 °C,
2
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