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Файл:Ординатура / Хирургия / Библиотека им академика М.И. Перельмана / Книга_5512_Библиотеки_им_академика_М_И_Перельмана.pdf
(I)
J497
(k)
FIGURE 19.7
a a
a
[Note
a
498
Medical Laborato Technology: Volume 2
Number of AF organism found in 10 elds
None
01
10100
>100
en positive, give actual number per 10 elds.
Repor
Negative
Positive (1+)
3+
4+
-
Note
leprae)
Introduction to Diagnostic Micbiolo
J499
Procedure
Nocardia
Reagent
°
Procedure
Albert's Stain
diphtheriae.
diphtheriae
Reagents
• Solution A: Albert's stain
500
Medical Laborato Technology: Volume 2
• Solution B: Albert's iodine stain
Procedure
isolation culture
medium
common normal ora
5
preliminary report direct examination conrmatory report laboratory culture
Culture Media
substrates
Introduction to Diagnostic Micbiolo
Broth media
Semisolid media Solid media
primary media secondary media,
Jso1
allow most organisms to grow
dierentiating between organisms
S. pyogenes S. aureus S.
pneumoniae. E.coli)
Shigella)
selective group of organisms to grow,
selective-dierential media
favour the growth
Shigella Salmonella.
so2 I
Shigella Salmonella
E. coli
Shigella Salmonella
Salmonella-Shigella
Medical Laborato Technology: Volume 2
Transport Media
Neisseria,
Haemophilus, Shigella
Neisseria Trichomonas vaginalis
Salmonella, Shigella Vibrio.
Biochemical Me and Tests
Preparation of Culture Media
pH Adjusent of Culture Mea
Introduction to Diagnostic Micbiolo
BLE 19.2
Jso3
Media and tests
EMB
Organism
Observation
Titration:
-
\
(
0)
-
�
(.
FIGURE 19.8
(b) Add 1 mL of phenol red indicator (0.02% w/v, aqueous solution).
(c) Titrate the medium with 0.1 N sodium hydroxide (dilute 1 N 1:10) taken from a
burette. Always add the titrant one drop at a time during titration, thoroughly mix,
observe the colour change and then add the next drop.
Introduction to Diagnostic Micbiolo
Jsos
Addition of alkali:
m
1
V m
Note
Storing of Culture Media
Caution
Procedure
so6 I
Note
Medical Laborato Technology: Volume 2
Making of ar Slants