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Файл:Ординатура / Хирургия / Библиотека им академика М.И. Перельмана / Книга_5512_Библиотеки_им_академика_М_И_Перельмана.pdf
J607
Disc Diffusion Method
FIGURE 20.15
a
(d)
(e)
Principle
°
minimal inhibitory concentration (MIC),
qualitative
Susceptible (S):
Resistant :
I
:
FIGURE 20.16
ote
a
°
Preparation of Antibiotic Discs
Jsog
Alternatively:
°
°
TABLE 20.4
Antibiotic
Desired stren of Procedure to prepare stock solution
stock solution
°
Disc potency
s10 I
TABLE 20.5
Specimens and organisms
Urine
Blood, wound exudates, pus, sputum
Staphylococcus
Gram-positive cocci (other than staphylococci) Ampicillin, Cephaloridine, Cooxazole, Erythromycin,
Most Gram-negative bacilli
Antibiotics tested
Ampicillin, Cotrimoxazole, Nalidixic acid, Nitrorantoin,
Sulphonamide, Kanamycin (or Cephaloridine or Tetracycline)
Ampicillin, Cotrimoxazole, Erythromycin, Penicillin (benzyl),
Tetracycline and Gentamicin (or Cephaloridine or Streptomycin)
Erythromycin, Fucidin, Penicillin (benzyl), Tetracycline,
Lincomycin (or Clindamycin), Cotrioxazole (or Gentaicin
or Novobiocin)
Penicillin (benzyl) and Tetracycline
Ampicillin, Cephaloridine, Cooxazole (or sulphonamide),
Streptomycin (or Kanamycin) and Tetracyline
-
(Note For Salmonella include Chloramphenicol)
Haemophilus
Pseudomonas aeruginosa
Clostridiu and anaerobic Gram-negative
bacilli
Ampicillin, Cephaloridine, Chloramphenicol, Cotrioxazole,
Sulphonamide, and Tetracycline
Carbenicillin, Colistin (pol
Ampicillin, Clindamycin, Fucidin, Pencilillin (benzyl) and
Tetracycline
ixin E)
-
Preparation of Inoculum
For a mixed culture (on primary plate)
For a Pure Culture om Secondary Plate)
Mueller-Hinton TSB (Trypticase soy
broth).
°
Standardization of lnoculum
McFarland standard,
8
Preparation of Standard
precipitate
). barium sulphate
2
4
8
2
Matching with the Standard
Inoculate the standardized bacterial suspension within 15 min.
Inoculation of Test Plate
S. pneumoniae,
Haemophilus Neisseria.
°
°
lid partly open
°
Procedure
°
s12 I
°
susceptible, inter
mediate resistant.
Additional Note
Medical Laborato Technology: Volume 2
Broth Dilution for Minimal Inhibitory Concentration Testing
quantitative
serially diluted in a broth
°
Mycobacterial Susceptibility Testing
J613
{a)
{b)
◊
MIC
FIGURE 20.17
a
°
°
Medical Laborato Technology: Volume 2
Streptococcus pyogenes, Staphylococcus aureus, Salmonella,
Shigella, Bordetella pertussis, Neisseria meningitidis, Haemophilus inuenzae, Klebsiella pneu
moniae, Escherichia coli, Serratia Borellia recurrentis.
Streptococcus pyogenes
20. l
Preparation of Indicator Solutions
Preparation of Culture Media and Biochemical Reagents
Amies ansport Medium
616 I
Medical Laborato Technology: Volume 2
Procedure for the Preparation of Reagent
°
Bacitracin Test
Group
Reagent
°
°
Test Procedure
Bile Esculin Agar
selective detection
Oxgall
Preparation of Reagent
°
40 g