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Файл:Ординатура / Хирургия / Библиотека им академика М.И. Перельмана / Книга_5512_Библиотеки_им_академика_М_И_Перельмана.pdf
Laborato Diagnosis of Parasitic Infections
Caution
Lol iodine solution %)
Preparation
Alternatively
Fresh solution o/iomersal
Procedure of xing stool specimen
mailing,
no leakage. smear xed in polyvinyl alcohol
Preparation of PVA-xed Smear
Reagent
Procedure to prepare slides r microscopic examination (Figure 22. 1 )
I
Medical Laborato Technology: Volume 2
� W;thio 30
FIGURE 22.1
r
/ !
Processing of stool specimen for intestinal pasite infecon
�
!
°
Direct examination of ecal material (Figure 22. 1 )
°
Direct examination of liquid stool (using thiomersal-iodine-formaldehyde or TIF mixture)
Laboratory mination of Stool
J689
FIGURE 22.2
Note
sgo I
Note
scolex
Bloody stool
amoebic dysentery.
Dark stool bleeding
Occult blood
Microscopic examination of formed stool
helminths eggs and larvae
size measurement
Medical Laborato Technology: Volume 2
Microscopic Measurements of Size
calibrated stage micrometre
Calibration
Measurement Procedure (Figure 22.3)
ocular micrometre
stage micrometre
J691
I I I
I
FIGURE 22.3
a
i
Example
=
=
692
FIGURE
ote
Laborato Diagnosis of Parasitic Infections
J693
Procedure for Direct Wet Mount
Note
Procedure for direct wet mount with iodine
Reagents
Preparation of saline-iodine mounti uid
A. Stock solution of iodine
B. Working solution of iodine
Sealant:
Procedure
nuclear structures
Chromatoid bodies
694
Medical Laborato Technology: Volume 2
Formed stool:
If
If
Bloody and mucus stool: If
if
If
Wet mounts of concentrated specimen
Motile forms
if
sedimentation procedure
otation meth
od,
the parasitic forms, which therefore rise to the surface. This is opposite to the sedimentation
method. In otation method, the solution used for the otation of parasite elements should
not be too concentrated; otherwise it causes shrinkage and distortion of the parasitic forms.
J695
ri
(d}
(h)
(b)
d
.
»
� "
' 7 �
(Q�]
(g)
�»
'·
"
1
FIGURE 22.5
a
a
I
Medical Laborato Technology: Volume 2
Procedure
Note
Reagents
Caution
Caution
Equipment
Instrument:
Glassware:
Supplies: