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Файл:Ординатура / Хирургия / Библиотека им академика М.И. Перельмана / Книга_5512_Библиотеки_им_академика_М_И_Перельмана.pdf
Laboto Diagnosis of Mycoc Infections J647
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FIGURE 21.1
Ideng characte of rious motic agents: (a) Monomorphic amentous fos
of mycoc agents, Monomohic yeast and phenoic morphs, (c) Theay
mohic temic moc agents, (d) Monomorphic oppounistic fungi
I
Medical Laborato Technology: Volume 2
Cptococcus neoformans.
Candida.
Candida albicans,
albicans
albicans
glabrata,
Candida glabrata
°
thermal dimorphism
Blastomyces dermatitidis, Histoplasma capsulatum, Coccidioides immitis
Sporothrix schenckii. S. schenckii,
Sporothrix schenckii
Tissue forms
declining defence s
Aspeillus Penicillium,
stems
y
°
Laboto Diagnosis of Mycoc Infections
Zygomycetes,
actinomycetes
J649
Cuent Delopment
Evaluation of Laboratory Methods
I
Specimen Collection and Processing
TABLE 21.2
Common specimens
Skin scrapings
Corneal scrapings
Exteal ear scrapings
Sputum (respiratory secretions)
Spinal uid
Pus, exudates
Mucocutaneous lesions
Vaginal exudates
Urine
Bone marrow
Throat swab
Likely organisms
Laboto Diagnosis of Mycoc Infections
Micros
orum.
p
Sputum
°
Pus and dates
Note
Cerebrospinal uid
C
tococcus neoformans
p
Other Specens
Laboratory Procedures
I
Medical Laborato Technology: Volume 2
zygomycosis.
uoresce
potassium hydroxide calcouor white
staining of smears
Wet Mount Procedures
wet mount
J653
FIGURE 21.2
a a
Wet Mount in Alkali Solution
Reagents
10% KOH (or NaOH):
Alkali-glycerine solution:
Procedure
Medical Laborato Technology: Volume 2
Results
Calcouor White with 10% KOH
Reagents
• Solution A: Potassium hydroxide reagent
• Solution B: Calcouor white reagent.
Procedure
Comment
India Ink Preparation of CSF
neoformans
Specimen
Principle
Cptococcus neoformans
Reagent
Pelikan India ink:
Procedure
J655
Cptococcus neoformans
Cptococcus neoformans
Cptococcus,
FIGURE 21.3
Diagnostic Findings of Direct Wet Mount
Microsporum
ichophyton endothrix
Trichophyton Epidermophyton
immitis.
Actinomyces
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Medical Laborato Technology: Volume 2
India ink pre
aration of CSF:
p
neoformans.
Laboratory Culture
Candida
Trichosporon Malassezia Fusarium
Requirements for Laboratory Culture
Sabouraud dextrose agar medium
Aspergillus Penicillium,
TABLE 21.3
Media used
Candida,
Common culture me used in laboto for ous mycoc Infecns
Infections
Candida
Candida,
-
air-borne.