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Файл:Ординатура / Хирургия / Библиотека им академика М.И. Перельмана / Книга_5512_Библиотеки_им_академика_М_И_Перельмана.pdf
°
H inuenzae N. meningitidis. Neisseria meningitidis
2
Haemophilus Neisseria
S. pneumoniae
M. l
rae,
must be handled carelly.
Mycobacterium l
rae
M. l
rae
M. tuberculosis,
rae
M. l
Specimen Coction
s88 I
Medical Laborato Technology: Volume 2
1.
It
It
Observations
No bacilli/100 elds
01/100 elds
1-9/100 elds
10100/100 elds
Above 9/eld (globi present)
,-
,_
,-
Report
Ne
g
l+
2+
3+
4+
ative
-
-
-
Caution
Clostridium, Bacteroides Actinomyces.
Closum
peringens;
tetani
Specimen Collection
Laboratory Investigation
peringens;
Clostridium
tetani
Clostridium.
Clostridium peringens
Clos
tridium tetani
Test Procedure for Litmus Milk
peringens,
sgo I
Indole Lecithinase (Egg Yolk Agar)
-
+
/-
+
-
peringens
tetani
• A =Acid; C = Clot; G = Gas
Litmus Mil Reaction
-
FIGURE 20.12
Js91
Actinomyces israelii blood agar plates
wound exudates
°
A. israelii
Acomyces
Specimen
Actinomyces,
Laboratory Identication
Identiing characters:
anaerobic jar.
Gram-positive anaerobe,
Biochemical reactions:
Bacus Anracis
Specimen
Laboratory identication
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Medical Laborato Technology: Volume 2
the organism growing in the presence of small concentrations of pen
icillin grows as a string of large, rounded cells. Bacillus anthracis
should be suspected whenever you isolate a large, Gram-positive aer
obic rod that is non-haemolytic and non-motile. Take great care in
handling this organism, as inhalation of the organism can lead to very
serious inction.
Bacteroides
This is an anaerobic Gram-negative rod, a major component of the normal ora of the colon.
As such, it is a common cause of anaerobic infections below the waist, including acute appen
dicitis, puerperal sepsis, abdominal wounds, intestinal abscesses and septicaemia following
abdominal trauma.
Specimen
Wound exudates, serous uid, spinal uid or other materials. Immediately place the speci
men in thioglycollate broth.
Laboratory Identication
Media:
Identiing characters:
Thioglycollate broth without anaerobic environment, blood agar
in an anaerobic jar.
Strictly anaerobic bacillus, non-spore-forming, with rounded
ends and occasional uneven staining. Selective media include
anaerobic Columbia CNA agar (contains Colistin and nalidixic
acid to inhibit Gram-negative aerobes), and anaerobic KV agar
(contains kanamycin and vancomycin to inhibit Gram-positive
organisms). The colonies on blood agar are non-pigmented and
semi-translucent without haemolysis. The organism grows well
in thioglycollate broth, producing diuse turbidity.
Bordetea Pertussis
This is the pathogen which causes whooping cough.
Specimen
Nasopharyngeal swab or a cough plate.
Laboratory Identication
Medium:
Identiing characters:
Bordet Gengou containing 15% blood.
Gram-negative, aerobic, minute, coccobacillus; colonies are pearly
and glistening, surrounded by a zone of haemolysis. The direct
uorescent antibody technique (FA) is rapid and conrmatory.
Boea
This is a genus of spirochaetes, as are Treponema and Leptospira. Infections associated with
Borrelia spp. are relapsing fever (Borrelia recurrentis, spread by lice) and Lyme disease (Borrelia
burgdorferi, spread by ticks). Vincent's angina is a type of gingivostomatitis caused by Borrelia
spp. in combination with anaerobic Gram-negative rods.
Specimens
Blood, throat swab (Vincent's angina).
Js93
Laboratory Identication
Brucea
Specimens
Laboratory Identication
Brucel/a
2
2
°
Bruce/la
Bruce/la
Citrobacter
Salmonella.
Specimens
Laboratory identication
Media:
Identiing characters:
Salmonella), ONPG
and
Medical Laborato Technology: Volume 2
Typical Laborato Report for Drug Susceptibility Test in Urinary Tract Iction
Citrobacter eundii
Closum
Clostridium (Clostridium tetani), peringens
botulinum).
Specimens
Laboratory Identication
Media:
Identiing characters:
Caution botulinum
tetani:
botulinum:
peringens:
Coebacteum Diphee
Specimens
Laboratory Identication
Media:
Jsgs
Identiing characters:
Specimen
Laboratory Identication
2
Specimen
Laboratory Identication
Media:
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Medical Laborato Technology: Volume 2
Specimen
Laboratory identication
Media:
Identiing characters:
2
ancisea Tularensis
Specimens
Laboratory Identication
Me:
ntiing characrs:
Legionella.
Haemophus inuenzae
Specimen
Laboratory identication
Meum:
Identiing charac:
S. aureus.
S. aureus,
Haemophilus
Haemophilus inuenzae
S. aureus
Klebsiea
Klebsi
ella pneumoniae.