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minimal inhibitory concentration (MIC).
Use of Clinical Bacteriology Laboratory by the Clinician
Js47
Basis of Grouping
Gram staining procedure
s4a I
Medical Laborato Technology: lume 2
Expected Results
Limitations of Gram Staining
Classification of Commonly Encountered Pathogenic Bacteria
SPECIFIC BACTERIA
Js49
staphylococcus streptococcus,
Staphylococci
Septococci
CAMP test test
(CAMP
y
I
Medical Laborato Technology: Volume 2
°
Gram-Positive Bacilli
Gram-Negative Bacilli
Escherichia coli
E. coli
Escherichia coli
Salmonella spp
cholerae.
brio cholera
Campylobacter
jejuni Campylobacter intestinalis
Helicobacter pylori,
Bacteroides agilis
Fusobacterium spp
Jss1
Respiratory Tract
inuenzae,
inuenzae
inuenzae
Legionella spp
Legionella Pneumophilia
L Pneumophilia,
Bordetella pertussis,
Bordetella pertussis
Pseudomonas
Pseudomonas aeruginosa
Pseudomonas aeruginosa
Burkholderia cepacia Pseudomonas cepacia)
Klebsiella pneumonia
552 I
Medical Laborato Technology: Volume 2
Spirochetes and Filamentous Bacteria
Zoonotic
Obligate Intracellular Organisms
Jss3
Organism with no Cell Wall
Mycoplasma
pneumoniae Mycoplasma hominis
In
normal ora
In
direct examination
laboratory culture.
Blood
Septicaemia
systemic inammatory response
In
bacteraemia
venepuncture
Blood Collection Procedure
FIGURE
a
FIGURE
Idencan of Pathogenic Bacteria
Jsss
sodium polyanethol sulphonate
if
°
aerobic anaerobic
If
if
common skin ora,
If
if
monophasic
biphasic
°
FIGURE 20.3
Biphasic medium (solid and
liquid) for blood cultu
Routinely blood cultures °if
Subculture
MacConkey agar plate
if
556 I
If
If
If
Laboratory Diosis of Bacraemia
normally sterile.
coagulase-negative
staphylococci
common oending organisms
Direct examination
Blood culture
antimicrobial susceptibility testing
presumptive identication
TABLE 20.1
Observation
Presumptive diagnosis
Streptococcus pneumoniae
Streptococcus pyogenes
Staphylococcus aureus
Bacteroides
Clostridium