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Файл:Ординатура / Хирургия / Библиотека им академика М.И. Перельмана / Книга_5512_Библиотеки_им_академика_М_И_Перельмана.pdf
Jso1
Procedure
1. Place the medium (with 1.5% agar) in test tubes, and sterilize by autoclaving. If tubes
contain sterilized medium, but cooled in an upright position (the agar surface does not
have a slope), rst melt the agar in boiling water bath and then proceed to the following
step.
2. Cool the medium in an inclined position. Increased inclination allows more surface;
however, if a butt is needed, make the slope at 20° angle or less.
Making of Culture Plates
For aerobic culture of an organism, the use of Petri dishes is convenient. In addition, the Petri
dishes allow the organism to be isolated in pure colonies from commensals. A culture plate
(Figure 19.9) of standard size contains 20-25 mL of sterile medium. Media are never sterilized
while in the Petri dish since agar will bubble out of the Petri dish.
(g)
°
FIGURE 19.9
1 Cover
(f)
a
Mium
Procedure
1. Sterilize the medium by autoclaving in an Erlenmeyer ask or in test tubes holding
20-25 mL of the medium.
I
°
°
Medical Laborato Technology: Volume 2
Techniques of Aseptic Transfers
Use of oculating Needle and Loop
Preparing Wire Loops in the Laboratory (Figure 19.10)
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(
C l
FIGURE 19.10
Flame Sterilization of Loop and Needle (Figure 19.11)
Note
°
Procedures of Aseptic Transfers
s10 I
(c)
FIGURE 19.11
a a
,
FIGURE 19.12
�
2
1
-
a
Asep Transfer using Loop
The technique of aseptic transfer of bacterial culture is shown in Figure 19.12. Holding the
loop in the ame of the Bunsen burner sterilizes the loop. The loop should glow red-hot
for a few seconds. After aming, make sure to slightly cool the loop before picking up
organisms from the inoculum culture (the culture that is to be transferred.) When trans
ferring a culture from a plate, cool the loop by touching on the very edge of agar. When
s12 I
Aseptic Transfer in Case of Test Tubes
(c\iP
(a)
FIGURE 19.13
Aseptic Transfer to Petri Dishes (Figure 19.14)
Js13
(a)
FIGURE 19.14
a
Procedure of Streaking on Primary Media
TABLE 19.3
,-
,_
Specimen site
BA
X
X
X
X
X
X
X
Commonly used media
CA
X X
X
X X
EMB
X
X
X
THIO
X
X
X
Medical Laborato Technology: Volume 2
Note
°
S. pyogenes Streptococcus
°
Streptococcus pyogenes
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FIGURE 19.15
a
a
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-
FIGURE a
a
a
FIGURE
°
lip of the tube is amed before and after entry