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Файл:Ординатура / Хирургия / Библиотека им академика М.И. Перельмана / Книга_5512_Библиотеки_им_академика_М_И_Перельмана.pdf
Laboto Diagnosis of Mycoc Infections
Actinomyces,
Preparation of Culture Media
Chlamydospore Agar
carbohydrate-rich medium albicans
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Cornmeal Agar
albicans.
Preparation
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lh.
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Note
Littman Oxgall Agar
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Preparation
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Medical Laborato Technology: Volume 2
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Note
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of Nocardia asteroides
Potato-Dextrose Agar
Preparation
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Rice-Grain Medium
albicans.
Trichophyton
Microsporum.
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Sabouraud Dextrose Agar (SAB)
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FIGURE
1
Preparation
1.Dissolve agar in 1000 mL of distilled water by heating.
2.While hot, add peptone and dextrose.
3.Boil gently until dissolved.
4.Adjust the pH to 6.0.
5.Dispense into culture tubes (20 mL) with cotton plugs or caps.
6.Sterilize by autoclaving (121 °C for 15 min).
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Medical Laborato Technology: Volume 2
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c
Note
Sabouraud Dextrose Agar with Antibiotics
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c
Sabouraud Dextrose Broth
Candida
Preparation
c
Microscopic Examination of Laboratory Cultures
wet mounts
teased mount,
Laboto Diagnosis of Mycoc Infections
Jss1
slide culture,
coverslip sandwich technique,
Wet Mount in Lactophenol Cotton Blue (LPCB)
Reagent
• Lactophenol Cotton Blue (LPCB)
Procedure (Figure 21.2)
Biochemical and Other Tests
Germ Tube Test
blastoconidia albicans germ tubes
albicans.
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Medical Laborato Technology: Volume 2
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Temperature Tolerance Test for Cryptococcus
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Urease Test
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Carbohydrate Utilization (assimilation) Test
sugar-starved
Dermatomycosis:
Tinea Pityriasis versicolor
Candidiasis
sporotrichosis,
mycetoma
Laboto Diagnosis of Mycoc Infections
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Dee
p
Characteris Features
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Medical Laborato Technology: Volume 2
aerial mycelium
conidiophores
conidia.
arthroconidia.
Identian Chaenge
carbohydrate assimilation and/or
fermentation morphologic features
Convennal proach and Cuent Delopment
earlier and less
invasive means of diagnosis,
Dermatomycosis
Epidermophyton, Microsporum Tricho
phyton. tinea.
direct examination
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FIGURE 21.5
of spore growth, and the skin scrapings might show branching, septate, hyaline hyphae.
When lamentous structures are found in specimens, laboratory culture of the organism
should be done. Sabouraud dextrose with antibiotics (cycloheximide and chloramphenicol) is
the appropriate medium. Cultures are maintained at 30
are visible within 5 days, but might take as long as 2 weeks for the study of sporulation fol
lowing subculture on carbohydrate rich agar medium (rice grain agar, potato dextrose agar).
a
a
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C under aerobic conditions. Colonies
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Medical Laborato Technology: Volume 2
Trichophon
ichophyton
Macroconidia
Hyphae
Trichophyton
rosporum
Microsporum uoresces
Microsporum
multiseptate
Microsporum audouinii
Epideophon
Epidermophyton
fast growing
Subcutaneous Mycosis
Sporo Schenck
Specimen
Laboratory Diagnosis
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