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Файл:Ординатура / Хирургия / Библиотека им академика М.И. Перельмана / Книга_5512_Библиотеки_им_академика_М_И_Перельмана.pdf
Quantitative (reporting the titre)
Note
J761
·- .
FIGURE 24.4
(Note
=
=
=
Note
1:64,
The highest diluon of serum to give occulation the ti.
=
=
=
. '
162 I
Medical Laborato Technology: Volume 2
Specimen No.
(Example)
A
B*
C'
D w
N = Non-reactive; W = Weakly reactive; R = Reactive;
• Shows prozone eect;
+ Needs further dilution to establish the titre value. It also shows the prozone eect.
1
2
1:1 1:2
R
w
w w R R
w w R R
-
N N N
3
1:4 1:8
N N
Tube No. (dilutions)
4
1:16 1:32
N N N N
6
5
w w
R R R R
N N N N
7
1:64
N N
8
1:128
9
-
Control
N
N
N
N
10
+
Control
R
R
R
R
Interpretation of RPR results
Titre
1
8
1
Laborato cedures in Serology
J763
Treponema Pallidum lgM ELISA
detection of IgM antibody
Contents of a typical kit
B. Reagents and equipment required but not provided in kit
°
20 x
°
°
Note
Medical Laborato Technology: Volume 2
Procedure
°
=
=
=
=
=
=
=
=
=
=
Note
=
=
=
Laborato cedures in Serology
Treponema Pallidum Haemagglutination Absorption Test
syphilis treponema pallidum haemagglutination absorption (TPH test
Principle
Reiter treponemes.
Reagents (taken from a typical test kit, contents may vary)
Microtitre plates:
Laboratory supplies not available in the kits
Procedure
Note
766 I
B
D
E
F
FIGURE 24.5
A
a
v
Observation and reporting [Figure 24.6(b)]:
Caution
TPHA test
Observations
Positive:
Negative:
Borderline:
Note
J767
{b)
FIGURE 24.6
a
absorption phase
(
I
Medical Laborato Technology: Volume 2
Screening tests for syphilis
Specic test (semi-conato)
Treponema pallidum
Febrile Agglutination Tests
Salmonella phi-O Salmonella paraphi-AH
Francisella tularensis
J769
FIGURE
j \ '
a
a
a
FIGURE
a a a
a a
a
no I
Medical Laborato Technology: Volume 2
/ test for Salmonea infection
enteric fever.
typhoid
paratyphoid vaccine
Principle
4. BH
Slide agglutination method
°