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238 Textbook of Pharmacognosy
Safrole (C10H10O2)
Elemicin (C12H16O3)
Camphene (C10H16)
Myristic acid (CH3(CH2)12COOH)
Myristicin (C11H12O3)
Terpinene (C10H16)
Sabinene (C10H16)
Dehydrodiisoeugenol (C20H22O4)
History of Nutmeg
The early history of nutmeg dates back to the 1st century A.D., and this is evident in the writings of Pliny, the Roman philosopher, who spoke of a tree bearing nuts with two flavours (the nutmeg and the mace) and it is believed that Roman priests have burnt nutmeg as incense but this is disputed. In Vedic writings (India), it was recommended for headache,
Systematic Pharmacognostic Study of Volatile Oils 239
fever and bad breath. The Arabian writings refer it to be good for stomach ailments and also as an aphrodisiac. The ancient Greeks and Romans used it as a brain tonic though it was rare and costly. It is an integral drug in Chinese medicine and was used for stomach pain and inflammation. In holistic medicine, it is an excellent liver tonic which helped to remove the toxins. It is learnt that Emperor Henry VI fumigated the streets of Rome with nutmegs before his coronation. Nutmeg has also been found in the Egyptian tombs. In middle ages it was brought to Europe by the Arabs through the Venetians. It was in the 6th century that Arab merchants brought the nutmeg to Constantinople. It was traded by Arabs and was sold to the Venetians for high prices. In the 14th century, half a kilogram of nutmeg costed as much as three sheep or a cow. The trade of nutmeg was taken over by the Dutch (1600) followed by the English after a war. The Dutch explorers in particular, Van den Broeke and Jan Pieterscoon took away the first batch of nutmegs from Banda islands, Indonesia (the first specific place of origin of nutmeg) in 1608, followed by its meteoric spread in Europe. The British East India Company brought it to Penang, Singapore, Sri Lanka and the West Indies. Nutmeg has been used since 7th century in China. The Chinese are responsible for our current knowledge of the medicinal properties of nutmeg. The nutmeg was also popular with Byzantine traders who purchased it from Arabs. It is believed that here the plant got its name from ‘mesk’ in Arabian, translated into French, as ‘noix nuguette’ and then it became ‘nutmeg’ in English. Earlier, it has been used for everything from the cramps in the stomach to plague. Nutmeg is known to have been used as a spice of high value in medieval cuisine and in the European markets in the preparation of flavourings, medicines and preservatives. In Elizabethan times, it was very popular as it was believed that nutmeg could ward off the plague. The national flag of Grenada bears a split-open nutmeg fruit on it. Connecticut of USA has a nickname as the Nutmeg state or Nutmegger as its traders carved out nutmeg from wood and sold. Arabs used nutmeg as an aid for digestion and also as an aphrodisiac. In Indonesia the pulp is used to prepare a jam, ‘selei buah pala’. It was believed that nutmeg possessed magical powers and it was recorded by a 16th century monk who advised young men to carry the vials of nutmeg oil to anoint their genitals for increased virility. It was also believed that inserting a nutmeg into the left armpit helps in attracting the admirers in social events. The nutmegs were also used as amulets to be protected against many dangers and evils, from boils to rheumatism to broken bones and misfortunes. According to European folklore, Pagans used nutmegs as a symbol of luck, money, health and fidelity.
Medicinal Uses
Both nutmeg and the mace have a similar taste; nutmeg is slightly sweeter while the mace has a delicate flavour. Nutmeg is an aromatic, stimulant
240 Textbook of Pharmacognosy
and carminative and in larger doses it is narcotic. The concrete oil is a rubefacient while the volatile oil is a stimulant and carminative. Mace is carminative and aphrodisiac while the wood is astringent. Nutmegs, maces and their oils are largely used for flavouring, condiments and as carminatives. It can be administered in delirium tremens and in insomnia. The pills are used as anodyne and astringent in dysentery. The churna is used as a sedative and antispasmodic in asthma, colic, neuralgia, menorrhagia, dysmenorrhoea, spasmodic cough and lumbago. The confection is a useful tonic for the heart, brain and sexual debility, incontinence of urine and general weakness. The ointment is a topical application for itching and irritable haemorrhoids. The concrete oil is used in ringworm infections and stimulates the growth of the hair. It is a good liniment for chronic rheumatism, paralysis and sprains. Mace is used in low fevers, humoral asthma and bowel complaints. The roasted mace and nutmeg are useful in chronic diarrhoea, flatulent colic and some forms of dyspepsia and obstructions of liver and spleen. Nutmeg has narcotic and anaesthetic action and peripherally has an irritant action which is due to myristicin. The hallucinogenic activity of the drug is due to myristicin and elemecin which are transmitted in human body to amphetamines. It is used as a spice.
Non-medical Uses
The pericarp of the fruit is used to make a jam, ‘Morne Delice’, in Grenada. The fruit is a source of a jam, ‘selei buah pala’ in Indonesia and is also used in the preparation of fragrant candies called ‘manisan pala’ (nutmeg sweets). It is a common constituent of cuisine all over the world. In Penang, it is made into pickles, ‘Ais Kacang’ and it is also blended or boiled to prepare iced nutmeg juice, ‘Lau Hau Peng’ in Penang Hokkien. In Indian cuisine, it is used in many sweet and savoury dishes, in particular the Mughalai cuisine, and in small quantities used in garam masala (North Indian spice mixture) and also sometimes smoked. It is used as a spice in savoury dishes in Middle Eastern cuisine, in cooking and the preparation of savoury dishes in Greece and Cyprus, in the preparation of potato and meat dishes along with soups, sauces and baked goods in European cuisine, is added to the vegetables like Brussels sprouts, Cauliflower and String beans in Dutch cuisine, an ingredient of curry powders in Japanese cuisine and in the production of drinks like Bushwacker, Painkiller and Barbados rum in the Caribbean.
Other Uses
The essential oil is used in perfumery and pharmaceutical industry as an oleochemical. It is a natural food flavouring substance in baked goods, syrups, beverages and sweets. It is also used in the cosmetic industry, such as in toothpastes and tobacco. In medicine, it is used in diseases related to the nervous and digestive systems as it is an analgesic, antiseptic,
Systematic Pharmacognostic Study of Volatile Oils 241
carminative, stimulant, etc. The oil is a powerful tonic, stimulates the orgasm and dissolves the muscular fatigue in addition to reviving the vital functions. Nutmeg butter which is expressed from the nut is a semisolid and reddish brown with a taste and the smell of nutmeg. About 75% of it is trimyristin which can be converted into myristic acid (14-carbon fatty acid) which can be used as a replacement for cocoa butter and can be mixed with cotton seed oil or palm oil and is used as an industrial lubricant. The butter can also be used to make candles. Myristicin poisoning induces convulsions, palpitations, nausea, dehydration and body pain and it is also a strong deliriant. Fatal poisoning in humans is very rare but it is deadly to pets and livestock even in small quantities. Nutmeg inhibits prostaglandin production and it may affect the foetus in large quantitites. Though it was considered to be an abortifacient, it is safe for culinary use.
Adulteration
The powder is often adulterated with exhausted nutmeg, corn meal, curcuma, powdered beans, ground olive pits and coconut shells. This is often substituted with Macassar nutmegs or Papuan nutmegs of Myristica argentea Warburg and/or with Bombay nutmegs or wild nutmegs of Myristica malabarica Lamarck. The mace is often adulterated with Bombay mace.
EUCALYPTUS
Synonyms
Eucalyptus tree, Blue gum Eucalyptus, Australian Fever Tree leaf, Tasmanian blue gum tree, Southern blue gum tree, Silver Mountain Gum tree, Iron Bark Tree, Bloodwood tree, Fuzzy box tree, Stubborn wood tree, Wooly-butt tree, Stringy bark tree (English), Folium Eucalypti, Feuilles d’ Eucalyptus or Gommier bleu (French), Eucalyptus Blatter (German), Blauwe gomboom (the Netherlands), Eulaliptus galkowy (Polski), Blauer Eukalyptus (Deutsch), Eucalipto (Espanol), Eucalitto (Italian), Ocalittu or Ocaritti (Sardu), Tailapatra or Tailaparna or Nilanirgasa (Sanskrit), Sugandhapatra, Nilgiri taila, Nilagiri tailam (Telugu), Yukeliptas (Hindi), Yukkali (Malayalam), Karpoor maram (Tamil).
Biological Source
Oil of Eucalyptus is distilled from the fresh or dry sickle-shaped leaves of various species of Eucalyptus particularly E. globulus Labillardiere (Family: Myrtaceae). The gum (Eucalyptus kino) is collected as an exudation from the stem and the oil is distilled from the leaves. There are more than 700 species, mostly native to Australia and a small number are found in New Guinea and Indonesia and Philippine archipelago. Outside Australia, only 15 species occur and 9 do not occur in Australia. The name ‘Eucalyptus’
242 Textbook of Pharmacognosy
comes from the Greek, ‘eucalyptos’ (eu and kaluptos) meaning ‘well-covered’ (describe the operculum) or ‘beautiful bark’. Other species used for the commercial production of the oil include E. cineorifolia, E. dives, E. dumosa,
Leaves Flower buds
Flowers
Fruits
Dry capsulesMature capsule
Systematic Pharmacognostic Study of Volatile Oils 243
E. goniocalyx, E. horistes, E. kochii, E. leucoxylon, E. oleosa, E. polybractea, E. radiata, E. sideroxylon, E. smithii, E. tereticornis, and E. viridis. E. globulus
was first collected by Jacques-Julien Houton de Labillardiere (1755–1834), a French Botanist, who came along with the French Expedition, on the South-East coast of Tasmania in 1792–1793 and was described by him in 1791–1794.
Geographical Source
The main habitat is Australia and Tasmania. The species which is indigenous to Australia is cultivated in the high lands of India, chiefly on the Nilgiris. The species are cultivated throughout tropics and subtropics including America, Southern Europe, Africa, the Mediterranean basin, the Middle East, Argentina, Brazil, Paraguay, China, Indonesia and the Indian subcontinent. Eucalyptus oils are produced in Portugal, South Africa, Spain, China, Brazil, Australia, California, Southern Europe, India and Paraguay.
Collection and Preparation
The leaves are collected from the older parts of the trees and dried to express the oil. There are two types of steam distillation, water/steam distillation and direct steam distillation. The former is an improved method in which the still contains a grid which keeps the plant material above the water level. The water is boiled below the charge and wet steam passes through the plant material. During the process of distillation, only very tiny molecules can evaporate, so they are the only one which leave the plant. These extremely small molecules make up the essential oil. This proccess involves the use of steam to percolate and vapourise out the essential oils fom the plant material, with the subsequent condensation of steam and the essential oil prior to the separation.
Steam distillation is a process for the materials that are temperature­sensitive (like the essential oils). The traditional method of steam distillation is still used around the world today. The plant material is loaded into the extraction chamber and compacted tightly. The steam is released into the bottom of chamber as the boiler heats the water, and starts to travel upward, thus, saturating the material. The steam impregnates the plant fibre causing it to release the oil molecules as a gas from the molecule channel. Then, the steam carries the gas to the condenser where it goes through a phase change condensation as it passes through the cooling process in the swan neck and liquifies into water and oil. The mixture of oil and water then flows into the separator where the oil can rise to the top of the water to be poured off into the containers which can be drained off. The vertical steam distillation offers the greatest potential for protecting the therapeutic benefits and the quality of the essential oils. Similarly, the temperature also has a distinct effect.
244 Textbook of Pharmacognosy
Characters
Macroscopic Characters
The leaf stalk or the petiole is twisted and the lamina is sickle-shaped. It measures 8–30 cm in length and 2–7 cm in breadth, coriaceous, bifacial with an acute or acuminate tip. The base is unequal, obtuse to round with an uneven or revolute margin. The colour is greyish-green to pale yellowish­green and glaucous, glabrous or glandular punctate. The venation is pinnate reticulate. The veins of the first order run to margin and anastamose parallel to the margin. The leaves are evergreen and covered with oil glands. It has an aromatic odour and bitter cooling taste.
Microscopic Characters
Anatomically, the upper and lower epidermis of the leaf is made of polygonal cells with thick cutinised outer walls and both the layers possess sunken stomata. The mesophyll is differentiated into palisade and spongy parenchyma. Within these tissues occur large, subglobular internal glands lined with secretory epithelium containing yellow eucalyptus oil. Some of the cells of spongy parenchyma contain rosette like aggregates of calcium oxalate. Fibrous pericycle occurs outside the vascular bundles in the region of the midrib and the petiole.
Chemical Constituents
The fresh leaves contain about 0.4–1.6% of volatile oil (the dried leaves about 1–3%) of which 54–90% is eucalyptol (C
O – 1,8-cineol, 1,8-Epoxy-
10H18
p-menthane, 1,3,3-Trimethyl-2-oxanicyclo(2,2,2)octane, Cajeputol) and balance being composed of eudesmol, alpha-pinene (2.8%), p-cymene (2.7%), aromadendrene (C15H24), cuminaldehyde, globulol, pinocarveol, d-pinene and other terpenes in addition to crystallizable resin, a bitter principle, tannin, cerylic alcohol, butyric aldehyde (C4H8O), valerianic aldehyde (C5H10O), phellandrine, eucalyptic acid and calcium oxalate. 48 components out of 70 of the oil have been identified. Eucalyptol which
Eucalyptus oil Crystals of eucalyptol
Systematic Pharmacognostic Study of Volatile Oils 245
Eucalyptol (C10H18O)
Globulol (C15H26O)
Butyricaldehyde (CH
(CH2)2CHO)
3
Eudesmol (C15H26O)
Pinocarveol (C10H16O)
comprises up to 90% of the essential oil is colourless liquid, cyclic ether and a monoterpenoid which has a density of 0.9225 g/cm2, a melting point of 1.5°C and a boiling point of 176–177°C. Eucalyptus oil is a collective generic name for the oils from the genus. The gum contains kino-tannic acid, catechin and pyrocatechin.
History of Eucalyptus
The Australian aboriginals use the infusion of eucalyptus leaves as a traditional medicine for body pains, sinus congestion, fever and colds. The botanical collections of Eucalyptus species have been made in 1770 by Joseph Banks and Daniel Soalnder with Captain James Cook at Botany Bay near the Endeavour River in Northern Queensland, Australia, though they have been seen by the European explorers. They have collected E. gummifera and E. platyphylla. David Nelson (1777) on Cook’s third expedition collected
E. obliqua (the French Botanist, L’Heritier coined the generic name, Eucalyptus, from the Greek roots eu (well) and kaluptos (covered), in
reference to the operculum of the flower bud which protects the developing parts of the flower and is shed at flowering; and the species obliqua from
246 Textbook of Pharmacognosy
Latin, obliquus referring to the unequal leaf base) on Brunny island in Southern Tasmania and took the specimen to the British Museum in London. The surgeons on the First Fleet, Dennis Considen and John White (1788) distilled eucalyptus oil from the leaves of E. piperita growing on the shores of Port Jackson to treat the convicts and marines. Subsequently, the oil was extracted by the early colonialists. Several more species were collected, named and published in the 19th century by James Edward Smith, an English Botanist, from the regions of Sydney like E. pilularis, E. saligna and E. tereticornis. The French Botanist, Jacques Labillardiere, collected the first endemic Western Australian species, E. cornuta from Esperance area in 1792. The first comprehensive account of the genus Eucalyptus was due to the work of Baron Ferdinand von Mueller, the German Botanist and explorer (the Director of the Botanical Garden, Melbourne, Australia, from 1857 to 1873), who made known the qualities of Eucalyptus all over the world and led to its introduction into Europe, North and South Africa, California and the nontropical South America. He was the first to suggest that the leaves might be of use as a disinfectant in malarial fever. He also promoted the disinfectant qualities of Eucalyptus and also encouraged Joseph Bosisto, a pharmacist from Melbourne, Australia, to investigate the commercial potential of the oil. In 1852, Bosisto set up a distillation plant to start the industry near Dandenong, Victoria, and extracted the oil from E. radiata. This later became the generic ‘oil of eucalyptus’ or ‘Bosisto’s Eucalyptus Oil’ and this brand still survives in the market. The details of Mueller’s work appears in Flora Australiensis of George Bentham (1867) and this remains the only complete Australian flora even today and is the most important early systematic treatment of the genus. In India, the plantations were made in 1863 in the Nilgiris at Ootacamund (Ooty). In 1870, F. S. Cloez, the French Chemist, identified and ascribed the name eucalyptol to the dominant portion of E. globulus (Tasmanian blue gum) oil, now referred to as cineole. By 1880s, the surgeons began using the oil as an antiseptic during surgeries. Bentham’s work was later elaborated by Joseph Henry Maiden (1903–1933) and by William Faris Blakely (1934). Eucalyptus has a long history in India. Around 1790, it was first planted by the ruler of Mysore, Tippu Sultan, in his palace garden on Nandi hills, near Bangalore, Karnataka. Subsequently, it was introduced in the Nilgiri hills, Ootacamund, Tamil Nadu, in 1843. From 1856, regular plantations were raised to meet the demand for firewood. The first plantation was raised at Malabavi (Devarayanadurga), Tumkur district by the Forest Department in 1877.
Medicinal Uses
Eucalyptus oil is a stomachic, carminative, expectorant, rubefacient, antiseptic, febrifuge, stimulant, diaphoretic, antimalarial and antiperiodic. It is much used for alleviating the symptoms of nasopharyngeal infections,
Systematic Pharmacognostic Study of Volatile Oils 247
for treating coughs and as a decongestant. It is taken internally in the form of mixtures, inhalations, lozenges and pastilles and applied externally as ointments and liniments. The oil increases the flow of saliva, gastric and intestinal juices thus aiding in appetite and digestion. It is used in the treatments of catarrh of the bronchopulmonary mucous membranes, intermittent and septic fevers, croup, diphtheria, whooping cough, purulent catarrh of genitourinary organs, surgical wounds, ulcers, etc. The leaves are chewed to clean the breath and harden the spongy and bleeding gums. The leaf infusion checks the secretions in bronchitis and coryza. The tincture is used in croup and in pulmonary gangrene. It is also useful in purulent catarrhal affections of the bladder, urethra and vagina, and in chronic cystitis with haematuria. The extract of the leaf is also used as a local application in erysipelas of the face, legs and scrotum. It also increases the heart beat, lowers the arterial tension and speeds up respiration. It is eliminated through perspiration (skin), urine (kidneys), bronchi (lungs) and milk. In higher doses, it is an irritant of the alimentary canal and produces eructation, indigestion, nausea, vomiting and purging. It is also a narcotic poison in toxic doses, which paralyses the respiratory centre in the medulla of the brain. It controls asthma and is used in mouthwashes and in rhinosinusitis. Topical application reduces inflammation and pain in headaches on bending, frontal headache, nasal obstruction, etc. The oil is an ingredient of ‘cathedral oil’ that is used for sterilising and lubricating urethral catheters.
Culinary and Other Uses
Eucalyptol is used as a flavouring agent, for fragrance and in cosmetics. It is used in baking goods, confectionery, meat, cigarettes and beverages. The oil is a natural insecticide and sometimes used to clean the swamps for reducing the risk of malaria. The oil is antibacterial and is used for cleansing, deodourising the respiratory tract, and in very small quantities in dental care, soaps, detergents, lotions, cough drops and decongestants. It also stimulates the immune system. The oil possesses insect repellant (disinfectant) properties which destroys the lower forms of life, and used as an ingredient in commercial mosquito repellants and also as a biopesticide. The plant is also used as an ornamental tree, for timber, firewood and pulpwood. It is used in industries like fence posts, charcoal, cellulose and biofuel. The plant shows a very fast growth and is suitable for windbreaks and to reduce erosion. It is also used for lowering the water table and thus reduces soil salination (as it draws large amounts of water by transpiration). The nectar is the source of high quality monofloral honey. The wood is used in the manufacture of a traditional Australian aboriginal wind instrument, digeridoos. All parts of the plant are used to make coloured dyes ranging from yellow and orange through green, tan, chocolate and deep rust red. In veterinary medicine, the oil is administered in influenza