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- •Preface to the Second Edition
- •Preface to the First Edition
- •Contents
- •Introduction
- •Pharmacognosy–Definition
- •Pharmacognosy–History
- •Animal Source
- •Pharmacognosy–Scope and Development
- •Review Questions
- •Plant (Vegetable) Source
- •Mineral (Earth) Source
- •Marine (Aquatic) Source
- •Microorganisms (Microbiological) Source
- •Review Questions
- •Alphabetical Classification
- •Morphological Classification
- •Taxonomical (Biological) Classification
- •Pharmacological (Therapeutic or Physiological orPharmacodynamical) Classification
- •Chemical Classification
- •Chemotaxonomic Classification
- •Serotaxonomical Classification
- •Review Questions
- •Introduction
- •Cultivation
- •Breeding for Improvement
- •Review Questions
- •Introduction
- •Botanical Identity
- •Specimens
- •Seeds and other Propagation Material
- •Cultivation
- •Site Selection
- •Climate
- •Soil
- •Irrigation and Drainage
- •Plant Maintenance and Protection
- •Harvest
- •Primary Processing
- •Packaging
- •Storage and Transport
- •Personnel
- •Documentation
- •Review Questions
- •Carbohydrate Derivatives
- •Agar
- •Pectin
- •Honey
- •Review Questions
- •Lipids
- •Simple Lipids
- •Neutral Fats
- •Fats and Oils
- •Waxes
- •Compound Lipids
- •Derived Lipids
- •Review Questions
- •Review Questions
- •Appendix
- •Subject Index

168 Textbook of Pharmacognosy
Stearic acid or octadecanoic acid CH3(CH2)16COOH or (C18H36O2)
Linoleic acid or octadecadienoic acid (C18H32O2)
Linolenic acid or octadecatrienoic acid
(CH2CH=CH)3(CH2)7COOH or (C18H30O2)
CH
3
Oleic acid or octadecenoic acid CH3(CH2)7CH=CH(CH2)7COOH
or (C18H34O2)
History of Linseed Oil
Common flax was one of the first crops domesticated by man, and is
thought to have originated in the Mediterranean region of Europe.
Archeological remains indicate that several plants were first cultivated
almost about the same time in Mesopotamia which included flax, emmer
wheat, barley, einkorn wheat, peas, lentils, chickpeas and bitter vetch. The
people of the Stone Age living near the Swiss Lake produced flax and
utilised the fibre and the seed. The linen cloth made from flax was used to
wrap the mummies in the early Egyptian tombs (5000 BC). The Babylonians
were the earliest people to cultivate flax as a source of food and irrigation
ditches were formed along the Tigris and Euphrates rivers of the Fertile
Crescent (2000 BC) to ensure a good water supply for the flax fields. Homer
(8th century BC) in his epic poem, the Iliad, wrote that linen was used for
cord and sail-cloth and this was an indication that the Greeks cultivated
flax plants and also consumed the seeds. The ‘Father of Medicine’,
Hippocrates (460–377 BC), also recognised the medicinal value of flax in
relieving many intestinal disorders upon prescription to patients. The early
colonists in the United States grew flax for their home use. The plant has
been known to be cultivated for its seed since very ancient times and it is
one of the oldest cultivated plants known to man. The 8th century King of
France, Charlemagne regarded flax very highly for its health benefits and

Systematic Pharmacognostic Study of Lipids 169
also made a detailed entry pertaining to the cultivation and use of flax for
food and medicine in his medical law books and also ordered his subjects
to consume it to maintain good health and also to prevent disease. The
excavations of the most ancient Egyptian tombs have come up with flax
seeds as well as the flax fibre cloth woven from the plant. The commercial
production of flax began in 1753. Even today, few still grow flax to make
linen for their own use. The first linen mentioned in the Bible was spun
from flax according to historians and archaeologists. The linseed oil has
been used medicinally by human societies since pre-history. It is believed
that the first ancestors of the human race as long ago as 8500 years ago, ate
the flax and some wild grasses as a part of their diet. In ancient times, the
diet of early humans contained every part of the flax plant.
The plant has been cultivated for its fibre, for at least five millennia. The
wall paintings of ancient Egypt depicted the spinning and weaving of linen.
The flax fibre was processed into a fine white fabric as early as 3000 BC and
this was used to wrap the mummies of the ancient Egyptian Pharaohs. It
has been also used as a cool and comfortable fiber in the middle east for
centuries, as mentioned several times in the Bible. It was also greatly valued
as a commodity by Ancient Greeks and Romans. The flax has been introduced
to Northern Europe by Finnish traders. It was known that the ‘Father of the
Nation’, Mahatma Gandhi once said, ‘whenever flax seeds become a regular food
item among the people, there will be better health’. Flax is the emblem of Northern
Ireland used by its Assembly. It also appeared on the reverse of the British
one pound coin to represent Northern Ireland in 1986 and 1991. Flax is also
seen on the badge of the Supreme Court of the United Kingdom representing
the Northern Ireland. Common flax is also the national flower of Belarus.
Medicinal Uses
The oil is used in liniments and also as a demulcent, emollient and laxative.
The oil is also an expectorant, diuretic. The seeds are aphrodisiac and the
roasted seeds are astringent. The mucilage from the seed extracted by cold
water is used for the irritable conditions of conjunctiva of the eye. Along
with honey it is used for cough and colds. The powdered seed cake is
called Linum contusum is popular as linseed meal. The poultice of the meal
is used as a soothing application to ulcerated and inflammed surfaces, boils,
carbuncles, abscesses, etc. It is also used as a mild counter-irritant for the
deep seated inflammations like pneumonia, bronchitis, bronchopneumonia, pleuritis, pericarditis, peritonitis, arthritis, pelvic cellulitis, etc.
Linseed oil is prescribed internally in ‘painter’s colic’, piles and a good
laxative. With lime water in equal proportions, it is a popular remedy for
burns and scalds as ‘carron oil’. The infusion of the seed called linseed tea
with licorice and sugar is internally used as a demulcent in cold, cough,
bronchitis, irritations of urinary organs, cystitis, gonorrhoea, stranguary,
as injection to the vagina, bladder and the rectum.

170 Textbook of Pharmacognosy
Other Uses
In addition, the stem fibres find a use in the manufacture of linen cloth.
The best quality lint is made from the retted and finely carded fibre. Linseed
oil is used as an impregnator in varnish and wood furnishing, as a pigment
binder in oil paints, as a plasticiser and hardener in putty and in the
production of linoleum. It is edible but is a minor constituent of nutrition
because of its strong odour. It is also used as a traditional protective coating
for the raw willow of cricket bats, for cue shafts (billiards) and surf boards.
Adulteration
The common adulterants are corn meal, wheat middlings, cake meal and
foreign weed seeds. It is also adulterated with cotton seed oil, sunflower
oil, resin, mineral oils, mustard oil and fish oils.
COCOA BUTTER
Synonyms
Theobroma oil, Cacao butter, Butter of cacao, Oleum Theobromatis,
Butyrum Cacao, Oleum concretum e Semine Theobromae Cacao, Beurre
de Cacao (French), Kakao butter (German).
Synonyms for Cocoa
Cocoa plant, chocolate tree, Chocolate, cocoa, Nicaraguan cocoa shade
(English), Cacaoboom (Dutch), Kakao (Finnish and Swedish), Cacaoyer,
Cacaotier, Cacao (French), Kakaobaum, Kakaopflanze (German), Cacao
amarillo, cacao criollo, Cacao forastero (Spanish), Keke shu (Chinese),
Cacau (Portuguese), Coklat (Indonesian), Maikona gaha (Sinhalese), Kona
maram, Kakkavo (Tamil), Kokkoo (Malayalam), Kho kho (Thai), Kokoe
(Burmese), Pokok coklat (Malaysian), Planta del cacao (Italian).
Biological Source
Cocoa butter is a fixed oil (fat) extracted from the roasted seeds (cacao
beans—about 40–50%) of Theobroma cacao L. (Family: Sterculiaceae).
Theobroma in Latin means the ‘food of the Gods’.
Theobroma cacao
—fruits
Mature fruits of cacao

Systematic Pharmacognostic Study of Lipids 171
Single fruit-unripe Ripe fruits—different stages
Dry fruits and beans
Cocoa beans
Cocoa chocolate
Split opened fruit

172 Textbook of Pharmacognosy
Geographical Source
Cocoa is produced in Ecuador, Colombia, Brazil, Venezula, Guiana, West
Indies, Indonesia, Malaysia, Dominican Republic (outside Africa) and
Cameroon, Nigeria, Ivory Coast and Ghana (West Africa).
Collection and Preparation
The cocoa seeds are separated from the pods and are allowed to ferment
wherein the seeds change their colour from white to dark reddish-brown
due to enzymatic reaction (chocolate liquor). Fermentation occurs in tubs,
boxes or cavities in the earth, the process lasts 3–9 days, and the temperature
is not allowed to rise above 60°C. The seeds are then roasted at 100–140°C,
when they lose water and acetic acid and acquire their characteristic odour
and taste. Roasting facilitates removal of seed coat. The seeds are cooled as
rapidly as possible and testa removed by a ‘nibbling’ machine. The nibs or
kernels are separated from the husk by winnowing. The kernels are then
fed to hot rollers which yield a pasty mass containing cocoa butter. This is
further purified to give cocoa butter. Butter can also be extracted by the
broma process. Finally, it is deodourised to remove its undesirable taste.
Characters
Cocoa butter is a yellowish white or a pale-yellow pure, edible vegetable
fat that has a mild chocolate flavour and aroma and bland chocolate-like
taste. It is brittle at temperatures below 25°C. It melts between 35 and 37°C.
The refractive index of the butter is 1.44556–1.44573. It has an iodine value
of 34.8, an acid value of 1.68 and saponification value of 197.7. The oil is
freely soluble in ether, chloroform and benzene and boiling dehydrated
alcohol and slightly soluble in 95% alcohol. Its saponification value is not
less than 188 and not more than 195. Its iodine value is not less than 33
and not more than 38. The butter displays polymorphism with crystals
possessing different melting points.
Cocoa butter Cocoa powder—as flakes

Systematic Pharmacognostic Study of Lipids 173
Raw cocoa nibs Cocoa powder
Chemical Constituents
The solid fixed oil consists chiefly of the glycerides of oleic, stearic and
palmitic acids. Because of its high content of oleostearin, it is also used as
a source of stearic acid. It is one of the stable fats known which contains
natural antioxidants that prevent rancidity. The percentage of the
constituents—non-saponifiables is 0.2–0.4%, total saturated fats is 57–64%,
free fatty acids is 1.08%, total monosaturated fats is 29–43% and total
polysaturated fats is 1–5%. The main fatty acids of the butter are represented
by oleic acid (29–38%), palmitic acid (22–29%), linoleic acid (1–4%), stearic
acid (24–37%), capric acid (0–10%), myristic acid (0–4%), arachidic acid
(1%), palmitoleic acid (0–2%), and linolenic acid (0–1%).
Capric acid or decanoic acid CH3(CH2)8COOH or (C10H20O2)
Arachidic acid or eicosanoic acid CH3(CH2)18COOH or (C20H40O2)
History of Cocoa
Cocoa butter has been used for centuries in Africa for its moisturising
properties and healing properties, where it has been used to protect and
also condition the skin which has been damaged by the sun and the wind.
Cocoa tree was originally named kakawa and according to historical records;
the people of Mesoamerican civilisation along the Mexican Gulf coast
cultivated the tree in 1000 BC. They used the beans for making drinks for

174 Textbook of Pharmacognosy
consumption by the elite class. Later, the tree came to be known as cacao
from proto-Mixe-Zoqueqn family of languages, during Olmec civilisation
(1150–300 BC). Later, this culture moved to Mayan civilisation by the Izapan
civilisation of the lowlands of Chialas of Mexico, Peten of Guatemala and
Belize. Historians believe that the word, chocolate or chocolatl originate from
the Nahuatl word, xocoatl or cacahuatl meaning ‘bitter water’. Some believe
that it is a combination of the Mayan words, choco and haa and the Nauhatl
term, atl which resulted in the term ‘chocolate’. There is also another theory
according to linguists that the word chicolatl originated from the word,
chico-li meaning ‘to beat’ or ‘to stir’ and not chocoatl. It was a Swedish who
gave the cocoa plant its binomial, Theobroma cacao which literally meant
the ‘food of the Gods’. During 450–500 AD, clay vessels were made for
drinking the chocolate, as a status symbol by Mayans. At the end of Mayan
civilisation (200 BC to 1550 AD) cocoa beans had become a major
Mesoamerican commodity. We know that chocolates are made from cocoa
beans that have been cultivated for millions of years in the South American
rainforests. The archaeological surveys account for the physical residues
of chocolate in some Mayan pots which suggest that Mayans drank
chocolate some 2600 years ago. It is also believed that the cocoa plant was
cultivated in the Amazon for more than 4000 years. Archaeological evidence
comes from the recovery of cocoa beans at Uaxactun, Guatemala (1947)
and also from the preserved wood fragments of the cocoa tree at Belize
sites (1981) and Pulltrouser area (1984). Similarly, the residue analysis from
the ceramic vessels from an early classic period (460–480 AD) tomb at Rio
Azul in North-Eastern Guatemala has revealed the presence of theobromine
and caffeine. History also accounts for the worshipping of the cocoa tree
by the Mayans and Aztecs who offered chocolate to the God as an offering.
The cocoa beans were brewed along with maize and capsicum which
resulted in a spicy bitter-sweet drink which after fermentation was used
for the ceremonies by Aztecs (1500 AD). Aztecs also treated cocoa beans as
currency and paid their taxes too, and the beans were used as a currency
throughout Mesoamerica by the time the Aztec empire fell (1376–1520 AD).
Mythically, the cocoa tree has been linked to Quetzacoatl, the God of
agriculture of Mexicans. Chocolate came to Europe in the 16th century by
a Spanish explorer, Don Hernan Cortes (1519). The tradition of drinking
hot chocolate began in Spain where the drink was served to travelers and
was a common drink with the nobility. Christopher Columbus introduced
the chocolate into Spain (1502). The cocoa tree was introduced to the Asia
in 1560 and to the Africa in 1590. The chocolate was served in the Spanish
court during 1600 to 1650 and it arrived in London in 1657. Later, the drink
entered France, and was introduced to England in the 17th century by Sir
Hans Sloane. Initially, the brew was sold as medicine by apothecaries in
England. C. J. Van Houton, a Dutch chocolate master invented the ‘cocoa
press’ (1828) which helped to squeeze out cocoa butter from the beans

Systematic Pharmacognostic Study of Lipids 175
through an alkalising process called ‘dutching’. The first large scale
production of chocolate bar was by J. S. Fry and Sons who introduced the
use of cocoa powder (1847). Later, in 1853, it was Cadburys who
manufactured the chocolate and became the supplier to Queen Victoria,
and emerged as one of the leading chocolate manufacturers in the
world as we know it today. The milk chocolate was introduced into the
market by Henri Nestle and Daniel Peter (1879) in Switzerland by blending
cocoa powder and condensed milk along with cocoa butter and cocoa
solids.
Medicinal Uses
Cocoa butter has nutritive, stimulant, antiseptic and diuretic properties.
The oil is used pharmaceutically in the making of suppositories and also
as excipient for pills (oral medicine in capsule form) and as an emollient it
is an ingredient of many products in cosmetics, soaps and lotions. It is also
recommended as a moisturiser for the prevention of stretch marks in
pregnant women, in the treatment of chopped skin and lips. It is a natural
preservative. Theobromine in the butter is a diuretic. It is used to prepare
biscuits, chocolate, bakery products, pharmaceuticals, ointments and
toiletries. It is an additive in cosmetics, shampoos and soaps. It is used to
add flavour, scent and smoothness to chocolate, tanning oil, topical lotions,
creams and soap. It is used in conditions of eczema and dermatitis as it
helps as a barrier between the skin and the environment and also helps in
retaining the moisture (body care lotions and moisturisers). The butter also
contains cocoa mass polyphenol (CMP) which inhibits the production of
immunoglobulin IgE known to aggravate the symptoms of dermatitis and
asthma. Butter when used in massaging the skin helps relieve stress, boost
the immune system and inhibits the growth of cells of cancer and tumours
in addition to preventing the heart disease and eases the arthritis and helps
treating conditions such as psoriasis, fibromyalgia and chronic fatigue
syndrome according to recent research. The butter is rich in vitamin E which
helps to soothe, hydrate and balance the skin and provides collagen
assisting with wrinkles (stretch marks in pregnant women) and signs of
ageing. The butter is a folk remedy for burns, cough, dry lips, fever, malaria,
rheumatism, snake bite and wounds.
Adulteration
Wax, stearin and tallow are the common adulterants in cocoa butter. Mano
kernel oil is used as a substitute.
Alternative to Cocoa Butter
The global production of cocoa is in decline due to the failure of the crop,
diseases and aging plantations which lead to fluctuations in the price and
the necessity for the cocoa industry to find high quality cocoa butter

176 Textbook of Pharmacognosy
alternatives. The studies in this direction explore the potential of a wild
mango, Mangifera sylvatica, an under-utilised fruit in South-East Asia, as a
new cocoa butter alternative (CBA). The analysis showed that the wild
mango butter has a light coloured fat with a similar fatty acid profile
(palmitic acid, stearic acid and oleic acid) and triglyceride profile (POP
[1,3-dipalmitoyl-2-oleoyl-glycerol], SOS [1,3-distearoyl-2-oleoyl-glycerol]
and POS [1-palmitoyl-2-oleoyl-3-stearoyl-glycerol]) to cocoa butter. The
thermal and physical properties of CBA are also similar to cocoa butter.
In addition, wild mango butter comprises 65% SOS (1,3-distearoyl-2-oleoylglycerol) which indicates its potential to become a Cocoa Butter Improver
(an enhancement of CBA).
KOKUM BUTTER
Synonyms
Goa butter, Kokum oil, Mangosteen oil, Black kokum, Cocum (French,
Spanish and Italian), Kokam (German), Kokum butter (Indian).
Synonyms for Garcinia
Red mango, Mate mangosteen, Indian berry, Indian tallow tree (English),
Kokam, Bhirand kokum (Hindi), Birandel ratamba, Kokambel, Kokan
(Gujarati and Maharashtrian), Murgal mara (Tamil), Murgina-huli-mara,
Dhupadamara, Murgala, Murginahalli, Punarpuli, Ratambi (Kannada),
Punampuli (Malayalam), Beerunda (Konkani), Brindao (pulp of the fruit)
or Amsel (bark of the tree) or Ratambasal (Goanese), Vrikshamla,
Vrichhamala (Sanskrit), Bhirand, Kokam, Kokambi (Marathi), Kokkam
(Malayalam), Garushinia indika (Japanese), Brindonnier (French).
Biological Source
Kokum butter is the fat expressed from the seeds of Garcinia indica (=Garcinia
purpurea), (Family: Clusiaceae=Guttiferae). The genus Garcinia is named
after Laurentiers (Jacques) Garcin (1673–1751), a French explorer, physician
Garcinia indica
Fruits

Systematic Pharmacognostic Study of Lipids 177
and a priest who lived in India and collected it as well as first described it.
He wrote the first article on the medicinal properties of the fruit in 1697.
Geographical Source
The plant is indigenous to Thailand, Cambodia and China. The plant is a
native to the western coastal region of Southern India. In India it grows in
the evergreen forests of Konkan (Mangalore), Malabar (Kerala) and Canara
(Karnataka) districts of Western India and Assam, Khasi, Jantia hills, West
Bengal and Gujarat.
Collection and Preparation
Raw kokum butter is a white coloured fat with a creamish-yellow or even
slight-grey tint which is extracted by traditional rural/cottage industry
sized oil mills. The fruits are collected manually by hand picking. The
branches are shaken with long sticks and the fallen fruits are collected.
These are broken by sticks to separate the seeds, picked up by hand. The
seeds are gathered from the natural forest habitats. The seeds are cleaned
to remove the dust, dirt and foreign matter. The seeds are dried to reduce
the moisture content. These are decorticated by wooden mallets. The final
product is obtained by crushing the kernels and boiling the pulp in water
(in a steam boiler) and skimming off the fat from the top or churning the
crushed pulp with water. Pure oil is obtained after double filtration and is
cooled to create kokum butter slabs and cubes of different sizes and
weight. This is a time consuming process and the yield is about 25% of
raw butter.
The kernels from the seeds are churned and boiled in water with caustic
soda. This yields hard soap which is decomposed by sulphuric acid leaving
tristearin as stearic acid, myristic acid and oleic acid. The melted fat is
separated by skimming with hot water twice and decolourised with animal
charcoal or Fuller’s earth. The seeds contain 30% of fat. After refining, the
fat is equivalent to vanaspati ghee.
Characters
This is a white to light grey or yellowish solid fat with a neutral odour and
taste. It is hard and brittle and exceptionally stable. It has an earthy
scent. It solidifies below 39°C and its melting point is 39–42°C. It has a
saponification value of 191 and an iodine value of 36.9.
Chemical Constituents
Kokum butter contains glycerides of stearic acid (40–45%), oleic acid
(40–50%), hydroxyl capric acid (10%), palmitic acid (5–8%) and linoleic
acid (2–4%). The fat is slightly bitter. It contains free fatty acids (0.08%),
non-saponifiables (0.9%), total saturated fats (52–68%), total monosaturated
fats (30–42%) and total polysaturated fats (0–2%). It also contains anti-
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