John Wiley & Sons - 2004 - Analysis of Genes and Genomes

468 INDEX

Smith, Michael, 234 Snaking, 94

Sodium dodecyl sulphate, 108 Somatic cells, 361

Somatic gene therapy, 397 SOS recruitment system, 227

South-Western blotting, 103, 216 Southern, Ed, 100

Southern blotting, 100, 155, 292, 293, 381 SP6 promoter, 124, 337

Spectinomycin, 351 Spliceosome, 57 Splicing, 55, 310

alternative splicing, 58

Spodoptera frugiperda, 270 Stable transfection, 363 Stahl, Franklin, 30

Stem cells, 324 Sticky ends, 75, 189 Strand selection, 237

Streptococcus pneumoniae, 3 Streptomycin, 62

Stringency, 101, 209 Stringent plasmids, 113 Structural genomics, 335, 339 STS maps, 295

Sturtevant, Alfred, 290 Subtraction libraries, 200 Sugar – phosphate backbone, 89 Sulphonylurea herbicides, 345 Supercoiling, 22

SV40, 367, 368, 378 early genes, 368 early promoter, 272 late genes, 368

SWI/SNF, 49 SYBR Green, 179

T-DNA, 342, 343, 346 T3, 124

T7 promoter, 124, 337

T7 expression system, 261

T7 lysozyme, 262

T7 RNA polymerase, 261, 262 TA cloning, 175

tac promoter, 259, 260 TAP-tagging, 286

Taq DNA polymerase, 155, 162 – 164, 171, 179, 200, 253, 254, 298, 316

Taqman , 180, 181 TATA box, 308

TATA-box binding associated factors (TAFs), 53

TATA-box binding protein (TBP), 51 Telomeres, 143, 307

Temin, Howard, 192 Terminal transferase, 195 Termination, 35 Terminator technology, 358

Tet expression system, 272, 378 tet operon, 273

Tetracycline, 62, 123, 273, 359 Tetracycline-inducible promoter, 329,

390

Tetracycline repressor (TetR), 227, 273, 358, 359

Tetracycline resistance, 117 TEV (Tobacco Etch Virus), 282

TEV protease, 281, 286

Thermostable DNA polymerases, 200, 243

Thermus aquaticus, 155, 162 Thiogalactoside transacetylase, 44 Three-hybrid screening, 228, 229 Thrombin, 282

Thymidine kinase, 376, 386 Thymine, 9

Ti plasmid, 341 – 345 disarmed, 343

Tissue-type plasminogen activator (t-PA), 208

Tn10 transposon, 273 Tn5 transposon, 376 Tn7 transposon, 272

Tobacco mosaic virus (TMV), 349, 350 Tomato golden mosaic virus (TGMV), 350 Topoisomerase, 34

Topology, 93 Transcription, 27, 42, 43

transcript profiling, 320 termination, 263, 378

Transcriptional activator, 219 Transcriptome, 313 Transfection, 362

Transfer plasmid, 270

Transformation, 3, 87 Transforming principle, 3 Transgene, 342 Transgene silencing, 347

Transient transfection, 362, 363 Transition mutations, 232, 254 Translation, 59, 378 Transversion mutations, 232, 254 Trisomy, 289

tRNA, 43, 263

SUP4, 145

Trophoblast, 381 trp operon, 260

TRP1, 143, 145, 222

Tumour suppressor genes, 315 Tus, 35

Two-dimensional gel electrophoresis, 275 Two-hybrid screening, 218, 224, 227 Two-step PCR mutagenesis, 242

UASG, 222, 224 ung, 239

URA3, 143, 145, 222 Uracil, 9

Uracil N-glycosylase, 239, 240 UV light, 207, 232

UV cross-linking, 100

Variable number tandem repeats (VNTR), 292

INDEX 469

Varshavsky, Alexander, 264

Vectors, 85, 111, 186

Vent DNA polymerase, 171

Vinograd, Jerome, 22

Viral resistance, 357

Viral transformation, 361

VP16, 228, 273

Waardenburg syndrome, 173

Wallace rule, 20

Watson, James, 12

Weak interactions, 225

Western blotting, 103, 214

X-Gal, 121, 270

X-ray crystallography, 336, 337

X-rays, 232

X-SCID, 399

Xenobiotic, 279

Xenopus laevis, 367, 381, 391

Xenotransplantation, 396

Xis, 39

Yeast artificial chromosome (YAC), 143, 295

Z-form DNA, 11

Zeocin, 123

Zinc cluster, 219, 244, 246

Zymolase, 105