Micro-Nano Technology for Genomics and Proteomics BioMEMs - Ozkan

536

STR loci, multiplex hybridization analysis of, 10–11 STR. See Short tandem repeats

Strand displacement amplification (SDA), 9, 17 Streptolysin O (SLO), poreforming bacterial toxin,

422

Striatial cholingeric internurons, extracellular potentials of, 58

Super ATM and Super TTM, 31 SuperbaseTM, 31

Superposition-TWD, 119 Surface activation

polyethylene, 165 polypropylene, 165 polystyrene, 164

Surface charge effects, 113–116

Surface modification, 162–163, 165, 173, 196, 321 γ-radiation, 164

adsorption of structured α-helical peptides, 173 corona treatment, 164

graft copolymerization, 164–165 hydrophilic surface modification, 165 hydroxy-functionalized surfaces, 167 Maleinimide-modified, 170 non-selective immobilization, 191, 193 silicon wafers, 142, 197

silylation, 164

Surface plasmon resonance (SPR), 130

Surface plasmon resonsance biosensor (BIAcore), 50 Surface tensin arrays, generalization of, 185

Surface tension arrays, generation of, 179, 185 Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry

(SELDI-TOFMS), 162 SYBR Green assay, 25

SYBR green DNA and ethidium bromide, intercalating dyes, 25

Syringe drive pumps, 312

Systems integration, challenges for, 380 challenges for sequencing human genome, 381 methodological challenges for sequencing, 381

T cells, 120

TWD velocity for, 119

T lymphocytes, membrane capacitance values of, 118

T lymphocytes, separation of, 120 TAMRATM, 28

Taq DNA polymerase, 5 -exonuclease activity of, 28

Taq DNA polymerase, exonuclease activity of, 28 TaqMan assay, 449

TaqMan expression profiles, 42 TaqMan hydrolysis probes, 29 TaqMan probes, 30

TaqMan real-time RT-PCR, 28 TaqMan real-time RT-PCR, 28–29

INDEX

target amplification and labeling, 37

single fluorophore experimental designs, 37–39 TASµ See Micro Total Analysis Systems

Tat peptide transduction, 423 Teichuronic acid, 114

Template DNA preparation, 373

Tetramethylrhodamine, 472 TETTM, 26

TGF-β gene in PBMC, expression level of, 13 TGF-β, expression level of, 13

TGF-β, expression levels of, increased, after LPS treatment, 13

TH01, STR loci, 11

Thermal denaturation profiles of window of discrimination, 410

Thermal expansion pumps, 312 Three-dimensional coatings

acrylamide gel pads, 173 agarose films, 173 gelatine pads, 173 hydrogels, 173

semi-wet gels, 173 Threshold cycle (CT), 28 Thrombosis associated SNPs,

factor V (Leiden), 10 factor II (prothrombin), 10

methylenetetrhydrofolate reductase (MTHFR), 10 Tiling-path arrays, 189

Time-resolved fluorescence polarization anisotropy(TR-FPA) techniques for torsional measurements, 489

Time-Resolved fluorometry, 437 TMR. See Tetramethylrhodamine TMR: FAM, ratio of, 472 TNF-α, expression level of, 13

TNF-α, expression levels of, increased, after LPS treatment, 13

Torsional measurements, TR–FPA techniques for, 489 TPOX, STR loci, 11

Transcription by the pol II system, 499 Transcription factors

helix-loop-helix, 478 helix-turn-helix, 478 leucine, 478

zinc finger, 478 “Transcriptome”, 24

Transducers in biosensors, types of electrochemical, 48 mass-sensitive, 48

optical, 48 thermometric, 48

Transport of tRNA-type ribozymes in cytoplasm, 504 Traveling Wave Dielectrophoresis, 118

junction of, 119 models of, 119

INDEX

Trypan blue dye, 65 TWD junction, 119

TWD velocity for T cells, 121

TWD. See Traveling Wave Dielectrophoresis Two fluorophore experimental design, 39

two fluorophore experimental designs, 39–40 applications, 40–41

gene expression profiling methods, comparison of, 41

cDNA arrays and other gene expression profiling methods, 41–42

oligonucleotide arrays and other gene expression profiling methods, 42–44

cDNA and oligonucleotide microarray expression profiles, 44

U937 cells expression levels of IL-1, TNF-α, and TGF-β, increased, after LPS treatment, 12–14

U937 cells, expression level of, 12–14

Vapor phase photo-grafting, 165 Variable angle spectral ellipsometry, 163

Vertical Cavity Surface Emitting Lasers (VCSEL), 153 Laguerre mode, 155

Very large scale immobilized polymer synthesis, 187 VIC R , 26

537

Visualization of physiological changes due to effect of chemical analytes effect of, 80

EDTA on neurons, 89 EDTA on osteoblasts, 91 ethanol on neurons, 80 ethanol on osteoblasts, 80

hydrogen peroxide on neurons, 83 hydrogen peroxide on osteoblasts, 84 pyrethroid on neurons, 86

pyrethroid on osteoblasts, 88 Vollum, sensitivity study for, 18

VX and soman (GD), chemical warfare agents, 57

Water-borne pathogens, in Salmonella, 409 Wavelength shifting reporter dyes, 408

X-ray photoelectron spectroscopy, 163

Yeast, 105, 107

Yeast, TWD theoretical model for manipulation, separation and characterization of, 119

Yeast, viable and unviable, DEP separation of, 65 Yersinia pestis (plague), DNA identification, 17

Zebrafish, 91

Zipper electrodes, 109