
- •Preface
- •Acknowledgements
- •Contents
- •List of Contributors
- •1. Array Formats
- •2. Biomolecules and Cells on Surfaces – Fundamental Concepts
- •3. Surfaces and Substrates
- •4. Reagent Jetting Based Deposition Technologies for Array Construction
- •5. Manufacturing of 2-D Arrays by Pin-printing Technologies
- •6. Nanoarrays
- •8. Labels and Detection Methods
- •9. Marker-free Detection on Microarrays
- •13. Analysis of Gene Regulatory Circuits
- •16. Biological Membrane Microarrays
- •18. Whole Cell Microarrays
- •20. Application of Microarray Technologies for Translational Genomics
- •Index

List of Contributors
Heinrich F. Arlinghaus
Physikalisches Institut der Universit¨at M¨unster Wilhelm- Klemm-Str. 10 D-48149 M¨unster, Germany arlinghaus@uni-muenster.de
Paul Bao
Nanosphere, Inc.
4088 Commercial Avenue Northbrook, IL 60062, USA pbao@nanosphere.us
Mark Basik
Translational Genomics Research Institute (TGen)
20 First Field Road Gaithesburg, MA 20878, USA mbassik@tgen.org
Ihab W. Botros
High Throughput Genomics, Inc. 6296 East Grant Road
Tucson, AZ 85712, USA ibotros@htgenomics.com
Konrad B¨ussow
Max Planck Institute of Molecular Genetics
Ihnestrasse 73
D-14195 Berlin, Germany buessow@molgen.mpg.de
Natasha Caplen
Medical Genetics Branch
National Human Genome Research Institute
National Institutes of Health Building 10, Room 10C103 10 Center Drive
Bethesda, MD 20892 USA ncaplen@mail.nih.gov
Alvaro Carrillo
Rensselaer Polytechnic Institute Howard P. Isermann Department of Chemical Engineering
Ricketts Building, 110 8th Street Troy, NY 12180, USA carria@rpi.edu
Linnette Demers
NanoInk, Inc.
1335 W. Randolph Street Chicago, IL 60607, USA ldemers@nanoink.net
Mitchel J. Doktycz
Life Sciences Division and Condensed Matter Sciences Division
Oak Ridge National Laboratory P.O. Box 2008
Oak Ridge, TN 37831-6123, USA doktyczmj@ornl.gov
XVIII List of Contributors
Andreas Eing
Nanofilm Technologie Anna-Vadenhoeck-Ring 5 D-37081 G¨ottingen, Germany aeing@nanofilm.de
Ye Fang
Corning, Inc.
Biochemical Sciences, Science and Technology Division
Corning, NY 14870, USA fangy@corning.com
Luisa Filipponi
Swinburne University of Technology Industrial Research Institute Swinburne
533-545 Burwood Road Hawthorn, VIC 3122, Australia lfilipponi@swin.edu.au
Anthony G. Frutos
Corning, Inc.
Biochemical Sciences, Science and Technology Division
Corning, NY 14870, USA frutosag@corning.com
Mona Fredriksson
Uppsala University Hospital
Dept Medical Sciences
S-751 85 Uppsala, Sweden
Mona.Fredriksson@medsci.uu.se
Viswanadham Garimella
Nanosphere, Inc.
4088 Commercial Avenue Northbrook, IL 60062, USA vgarimella@nanosphere.us
Kunal V. Gujraty
Rensselaer Polytechnic Institute Howard P. Isermann Department of Chemical Engineering
Ricketts Building, 110 8th Street Troy, NY 12180, USA gujratyk@rpi.edu
David S. Ginger
Department of Chemistry University of Washington Box 351700
Seattle, WA 98195-1700, USA ginger@chem.washington.edu
J¨orn Gl¨okler
Max Planck Institute of Molecular Genetics
Ihnestrasse 73
D-14195 Berlin, Germany gloekler@molgen.mpg.de
Chad Greef
SomaLogic, Inc.
1745 38th Street Boulder, CO 80301, USA
chad.greef@somalogic.com
Karl-Otto Greulich
Institute for Molecular Biotechnology Department of Single Cell and Single Molecule Techniques Beutenbergstrasse 11
D-07745 Jena, Germany kog@imb-jena.de
Piotr Grodzinski
Microfluidics Laboratory, PSRL, Motorola Labs
7700 S. River Parkway
Tempe, AZ 85284, USA Current address:
Bioscience Division, MS J586 Los Alamos National Laboratory Los Alamos, NM 87545, USA piotrg@lanl.gov
Kristi L. Hanson
Swinburne University of Technology Industrial Research Institute Swinburne
533-545 Burwood Road Hawthorn, VIC 3122, Australia khanson@swin.edu.au
Yulong Hong
Corning, Inc.
Biochemical Sciences, Science and Technology Division
Corning, NY 14870, USA hongy@corning.com
Claus Hultschig
Max Planck Institute of Molecular Genetics
Ihnestrasse 73
D-14195 Berlin, Germany hultschi@molgen.mpg.de
Anne Kallioniemi
University of Tampere Laboratory of Cancer Genetics Institute of Medical Technology P.O. Box 607
FIN-33014 University of Tampere, Finland anne.kallioniemi@uta.fi
Olli Kallioniemi
Medical Biotechnology Group
VTT Technical Research Centre of
Finland
University of Turku
P.O. Box 106, 20521 Turku, Finland
Olli.Kallioniemi@vtt.fi
Ravi S. Kane
Rensselaer Polytechnic Institute Howard P. Isermann Department of Chemical Engineering
Ricketts Building, 110 8th Street Troy, NY 12180, USA kaner@rpi.edu
Ravi Kapur
Anudeza Group 292 Morton Street
Stoughton, MA 02072, USA rkapur@anudezagroup.com
List of Contributors XIX
Joydeep Lahiri
Corning, Inc.
Biochemical Sciences, Science and Technology Division
Corning, NY 14870, USA lahirij@corning.com
Ralf Lenigk
Microfluidics Laboratory, PSRL, Motorola Labs
7700 S. River Parkway
Tempe, AZ 85284, USA Current address:
Applied NanoBioscience Center P.O. Box 874004
Arizona State University
Tempe, AZ 85287, USA
Ralf.Lenigk@asu.edu
Zirong Li
Ludwig Institute for Cancer Research UCSD La Jolla Medical School Campus
9500 Gilman Drive
La Jolla, CA 92093-0653, USA z3li@ucsd.edu
Peter Lichter
Molekulare Genetik
Deutsches Krebsforschungszentrum D-69120 Heidelberg, Germany p.lichter@dkfz.de
Ulrika Liljedahl
Uppsala University Hospital
Dept Medical Sciences
S-751 85 Uppsala, Sweden
Ulrika.Liljedahl@medsci.uu.se
Robin H. Liu
Microfluidics Laboratory, PSRL, Motorola Labs
7700 S. River Parkway
Tempe, AZ 85284, USA Current address:

XX List of Contributors
Applied NanoBioscience Center
P.O. Box 874004
Arizona State University
Tempe, AZ 85287, USA
Robin.Liu@asu.edu
Yingjie Liu
Microfluidics Laboratory, PSRL, Motorola Labs
7700 S. River Parkway
Tempe, AZ 85284, USA
Current address: Applied NanoBioscience Center P.O. Box 874004 Arizona State University Tempe, AZ 85287, USA
Jason liu@asu.edu
Sudhakar S. Marla
Nanosphere, Inc.
4088 Commercial Avenue Northbrook, IL 60062, USA smarla@nanosphere.us
Ralph R. Martel
High Throughput Genomics, Inc. 6296 East Grant Road
Tucson, AZ 85712, USA rmartel@htgenomics.com
Mirlacher Martina
University of Basel
Institute of Pathology
Schoenbeinstrasse 40
4031 Basel, Switzerland
Martina.Mirlacher@unibas.ch
Chad A. Mirkin
Northwestern University Institute for Nanotechnology 2145 Sheridan Road Evanston, IL 60208, USA mirkin@northwestern.edu
Spyro Mousses
Translational Genomics Research Institute (TGen)
20 First Field Road Gaithesburg, MA 20878, USA smousses@tgen.org
Uwe R. M¨uller
Nanosphere, Inc.
4088 Commercial Avenue Northbrook, IL 60062, USA umuller@nanosphere.us
Michelle Nessling
Molekulare Genetik
Deutsches Krebsforschungszentrum D-69120 Heidelberg, Germany m.nessling@dkfz.de
Dan V. Nicolau
Swinburne University of Technology Industrial Research Institute Swinburne
533-545 Burwood Road Hawthorn, VIC 3122, Australia dnicolau@swin.edu.au
Roeland Papen
Picoliter inc.
231 S Whisman Road, Mountain View CA 94041
roeland.papen@picoliterinc.com
Bernhard Radlwimmer
Molekulare Genetik
Deutsches Krebsforschungszentrum D-69120 Heidelberg, Germany b.radlwimmer@dkfz.de
Ramesh Ramakrishnan
Nanosphere, Inc.
4088 Commercial Avenue Northbrook, IL 60062, USA rramakrishnan@nanosphere.us
List of Contributors XXI
Bing Ren
University of California, San Diego Department of Cellular and Molecular Medicine, School of Medicine 9500 Gilman Drive, La Jolla, CA 92093-0653, USA
biren@ucsd.edu
Jan Roegener
University of Bielefeld
Department of Applied Laser Physics Universitaetsstrasse 25 D3
D-33615 Bielefeld, Germany roegener@physik.uni-bielefeld.de
Simon Ronald
University of Basel
Institute of Pathology
Schoenbeinstrasse 40
4031 Basel, Switzerland
Ronald.Simon@unibas.ch
Matthew P. Rounseville
High Throughput Genomics, Inc. 6296 East Grant Road
Tucson, AZ 85712, USA mrounseville@htgenomics.com
Guido Sauter
University of Basel Institute of
Pathology Schoenbeinstrasse 40 4031
Basel, Switzerland
Guido.Sauter@unibas.ch
Peter Schellenberg
Institute for Molecular Biotechnology Department of Single Cell and Single Molecule Techniques Beutenbergstrasse 11
D-07745 Jena, Germany schelli@imb-jena.de
Carsten Schwaenen
Medizinische Klinik der Universit¨at Ulm
Innere Medizin III D-89081 Ulm, Germany carsten.schwaenen@medizin.uniulm.de
Bruce E. Seligmann
High Throughput Genomics, Inc. 6296 East Grant Road
Tucson, AZ 85712, USA bseligmann@htgenomics.com
Drew Smith
SomaLogic, Inc.
1745 38th Street Boulder, CO 80301, USA
drew.smith@somalogic.com
James J. Storho
Nanosphere, Inc.
1818 Skokie Boulevard Northbrook, IL 60062, USA jstorhoff@nanosphere.us
Hans Martin. Striebel
Institute for Molecular Biotechnology,
Department of Single Cell and Single Molecule Techniques Beutenbergstrasse 11 D-07745 Jena, Germany
hms@imb-jena.de
Ann-Christine Syv¨anen
Uppsala University Hospital Dept Medical Sciences S-751 85 Uppsala, Sweden
Ann-Christine.Syvanen@medsci. uu.se
Matthias Vaupel
Nanofilm Technologie Anna-Vadenhoeck-Ring 5 D-37081 G¨ottingen, Germany mv@nanofilm.de
XXII List of Contributors
Gerald Walter
Biorchard AS Nedre Skogvei 14
N-0281 Oslo, Norway gerald@biorchard.com
Brian L. Webb
Corning, Inc.
Biochemical Sciences, Science and Technology Division
Corning, NY 14870, USA webbbl@corning.com
Hendrik Weiner
Max Planck Institute of Molecular Genetics
Ihnestrasse 73
D-14195 Berlin, Germany weiner@molqen.mpg.de
Swen Wessendorf
Medizinische Klinik der Universit¨at Ulm
Innere Medizin III D-89081 Ulm, Germany
swen.wessendorf@medizin.uniulm.de
Part I
General Microarray Technologies