Добавил:
kiopkiopkiop18@yandex.ru t.me/Prokururor I Вовсе не секретарь, но почту проверяю Опубликованный материал нарушает ваши авторские права? Сообщите нам.
Вуз: Предмет: Файл:
Ординатура / Хирургия / Библиотека им академика М.И. Перельмана / Книга_5643_Библиотеки_им_академика_М_И_Перельмана.pdf
Скачиваний:
0
Добавлен:
15.09.2026
Размер:
14 Мб
Скачать
☆
214 Herbal Pharmacopeia
Nanotechnology is the manipulation of matter at the atomic and molecular levels, usually at scales smaller than 100 nanometers. At this size, materials possess different properties, biological, physical and chemical, that differ dramatically from their bulk counterparts. These qualities can be used to enhance the administration and efcacy of herbal medications (Harika etal., 2021; Mishra etal., 2022). Uniting nanotechnology with herbal pharmacology is a process to generate nanoformu­lations that improve the medicinal effects of herbal substances (Ansari etal., 2012). These nanofor­mulations can comprise nanoparticles, nanospheres, nanoemulsions, liposomes, and dendrimers, among others. Such formulations can improve the solubility and stability of herbal substances, pre­serve them from degradation, increase their absorption and bioavailability, and allow targeted distri­bution to specic tissues or cells. (Harika etal., 2021).
Nanotechnology, a revolutionary discipline at the crossroads of physics, chemistry, biology, and engineering, has transformed several scientic elds since its inception. The ability to modify materials on the nanoscale (1 to 100 nanometers) has created new opportunities for invention and application, particularly with regard to medicine and pharmacology (Gavhane etal., 2021). Herbal pharmacology, which relies on the medicinal characteristics of plants and their derivatives, appears to benet greatly from nanotechnology advances (Mishra et al., 2022). This chapter investigates the synergistic potential of incorporating nanotechnology into herbal pharmacology, with the objective of rening the efcacy, bioavailability, and delivery of herbal medications (Harika etal., 2021). The intersection of nanotechnology and herbal pharmacology offers hope for addressing these obstacles. Nanotechnology can increase the transport, efcacy, and safety of herbal medicines by creating nanoscale formulations (Gopi etal. 2016). Nanoparticles, nanocap­sules, nanoemulsions, and nanogels can encapsulate herbal extracts or active chemicals, keeping them from degradation, increasing solubility, and allowing for regulated and targeted release (Rushikesh etal., 2021).
Many phytochemicals have low water solubility and are rapidly metabolized in the body, limiting their therapeutic efcacy. Nanotechnology can help solve this problem by enhancing the solubility and stability of these chemicals (Afroj etal., 2021). Curcumin, which is derived from turmeric, has limited bioavailability due to its low solubility and quick metabolism. Nanoparticle formulations of curcumin have demonstrated higher bioavailability, longer circulation duration, and improved thera­peutic efciency in numerous disease types (Ruturaj etal., 2023).
Nanotechnology is used for the delivery of herbal medications to precise cells and thereby improving therapeutic outcomes (Bruna etal., 2014). This is especially important in cancer treat­ment, as targeted delivery might boost anticancer chemical accumulation in tumour tissues while preserving healthy cells (Ruturaj etal., 2023). Nanoparticles loaded with herbal extracts, such as paclitaxel from the Pacic yew tree, have shown specic targeting and signicant anticancer action against a variety of cancer cell lines (Bruna etal., 2014). Nanotechnology enables the creation of delivery systems that provide continuous and controlled release of herbal ingredients (Sahu, 2013).
This can keep therapeutic concentrations of the medicine in the body for longer periods of time, lowering the frequency of delivery and enhancing patient compliance (Rushikesh et al., 2021). Nanocapsules and nanogels, for example, can encapsulate herbal extracts and slowly release them over time, maintaining a consistent supply of active components (Sharma, 2014).
The human body contains various biological barriers that limit the efcacy of herbal medications. These include the gastrointestinal system, the blood–brain barrier, and cell membranes. Nanotechnology can aid herbal substances in overcoming these obstacles (Arin et al., 2019). Nanoemulsions, for example, can improve the absorption of herbal components in the gastrointesti­nal tract, whilst liposomes can help transfer medicinal molecules across the blood–brain barrier (Vaibhav etal., 2020).
Numerous case studies show the successful use of nanotechnology in herbal pharmacology (Bruna etal., 2014). Nanoformulations of ginseng, a traditional herbal treatment, have demonstrated improved pharmacokinetics and therapeutic efcacy in alleviating exhaustion and improving immu­nological function. Similarly, green tea polyphenol nanoparticles have shown increased anticancer
Applications of Nanotechnology in Herbal Pharmacology 215
and anti- inammatory activity (Afroj etal., 2021; Mishra etal., 2022).While using nanotechnology into herbal medicine has numerous advantages, it also presents safety and regulatory concerns (Ansari etal., 2012). Nanoparticles’ unique features, such as their small size and large surface area, can cause unexpected toxicological effects (Chakraborty etal., 2016). Rigorous testing and evalua­tion are required to assure the safety and efcacy of nano- herbal compositions. Regulatory systems must develop to handle the problems posed by these innovative products, ensuring that they meet safety requirements and are supported by scientic evidence (Rupali, 2022).
The future of nanotechnology in herbal pharmacology seems bright, with continuing research and development targeted at improving nanoformulations and discovering new therapeutic applica­tions (Hiwa etal., 2020). Nanotechnology advancements are projected to result in more complex and multifunctional delivery methods, such as stimuli- responsive nanoparticles that release their payload in reaction to specic physiological situations (Ansari etal., 2012). In addition, integrating nanotechnology with other cutting- edge sciences, such as biotechnology and synthetic biology, could further boost the medicinal potential of herbs (Hiwa etal., 2020).

10.2 TYPES OF NANOMATERIALS UTILIZED IN HERBAL PHARMACEUTICALS

Employing nanotechnology revolutionized the contemporary and improvised approach of pharma­ceutical distribution and reaches peak efcacy with minimal toxicity used conventional herbal prod­ucts. The nanomaterials were utilized to enhance properties such as solubility, bioavailability, and the targeting ability of herbal substances (also described in Figure 10.1). The types of these engi­neered nanomaterials are nanoparticles, nanolayers, micelles, nanocapsules, and carbon- nanotubes. That constitutes some nanomaterials explaining their characteristics and uses, mainly in the eld of herbal drugs (Table 10.1).

10.2.1 NaNoparticles

Nanoparticles are solid colloidal particles that range in size from 1 to 100 nanometers. They improve the solubility, stability, and bioavailability of medicines by acting as carriers. Nanoparticles could better encapsulate poorly soluble plant extracts, perhaps improving the therapeutic efcacy of herbal medications (Sandhiya & Ubaidulla, 2020). Coupling is thus considered one of the chief advantages
FIGURE 10.1 Innovative pharmaceutical solutions for improved treatment.
216 Herbal Pharmacopeia
TABLE 10.1 Different Nanomaterial Types, Their Attributes, and Applications in Herbal Medicines
Type of Nanomaterials Properties Herbal Pharmaceuticals Application References
Nanoparticles A greater dose
proportionality, increased bioavailability, reduced dose form, Decreased toxicity Diminished fed or rapid variability
Nanoemulsions Regulating the
gradual release of active substances while ensuring their safeguarding.
Nanocapsules Protection and
controlled release of active substances
Nanoliposomes Efcient
encapsulation of drugs using biocompatible materials.
Nanobers High porosity,
controlled release of drugs
Quantum Dots High stability and
uorescent
Curcumin, Paclitaxel,
Berberin, Camptothecin, Ginkgo biloba, Triptolide, Salvia miltiorrhiza, Quercetin, Breviscapine, Naringenin, Dodder, Silymarins, Genistein, Centella asiatica, Annual mugwort
Furocoumarin Psoralen,
Curcumin, Triptolide
Dutasteride, Olanzapine,
Clarithromycin, Bedaquiline, Quercetin, Carvedilol
Curcumin, Catechins
constituents (catechin, epicatechin, epigallocatechin­3-gallate), SilymarinConstituents (silybin,taxifolin, isosilybin,silydianin, silychristin)
Nigella sativa (seed),
Satureja mutica
(seed), Flax (seed), Turmeric (root), Cissus quadrangularis (stem), Aloe vera (leave), Citrullus colocynthis (fruit).
Quantum dots, PEG-
encapsulated QDs, QDs encapsulated in phospholipid micelles.
Actions to counteract cognitive
decline, including memory loss, impaired reasoning, language difculties, and behavioral changes, Actions targeting the prevention of memory loss, cognitive decline, linguistic impairment, behavioral changes, as well as inammatory and immunological conditions. Medical conditions, particularly rheumatoid arthritis, are often associated with illnesses. Antioxidant, anxiolytic, and antimalarial.
This medication is used to
treat autoimmune disorders, including rheumatoid arthritis, psoriasis, leukaemia, and to exhibit antineoplastic effects.
Used to produce a signicant
amount of drug content for both water- soluble and oil- soluble herbal drugs.They increase the efcacy of herbal treatments by employing precise and sustained delivery techniques.
The results include
chemoprevention, anticarcinogenesis, antiviral effects, antioxidation, anti- obesity effects, anti- inammatory effects, antidiabetic effects, antimutagenesis, antiangiogenesis, antibacterial effects, and anti- aging effects.
antioxidant, anti- inammatory,
anticancer, analgesic, blood pressure- lowering, anxiety­relieving, angiogenesis, nerve repair, Insecticide, analgesic, anesthetic, fungicide, antioxidant, and antibiosis
Use in immunity tests to evaluate
changes in protein structure and how proteins interact with one another.
(Chakraborty
etal.,
2016)
(Thapa etal.,
2013)
(Deng etal.,
2020)
(Thapa etal.,
2013)
(Liu etal.,
2023)
(Sandhiya &
Ubaidulla,
2020)
(Continued)
Applications of Nanotechnology in Herbal Pharmacology 217
TABLE 10.1 (CONTINUED) Different Nanomaterial Types, Their Attributes, and Applications in Herbal Medicines
Type of Nanomaterials Properties Herbal Pharmaceuticals Application References
Dendrimers High loading
capacity, branched
Solid Lipid
Nanoparticles
Polymeric
Nanoparticles
Carbon
Nanotubes
Biocompatible,
biodegradable, and capable of encasing lipophilic medications
Enhanced surface
area, higher bioavailability
High strength and
ability to conduct electricity
Isothiocyanate, Triglyceride
of docosahexaenoic acid, Glucosamine, Capsaicin, N- Acetylcysteine
Triptolide, Podophyllotoxin,
Curcuminoids, Tetrandrine, Cryptotanshinone
Triptolide, Curcumin,
Camptothecin, Hypericin
Oridonin, Camptothecin,
Gallic acid derivatives, Vinblastine, Betulinic acid
With their unique polyvalency
and nanoencapsulation feature, dendrimers provide a novel approach to solubilizing these components and facilitating the transport of active biomolecules.Dendrimers regulate the release of loaded phytochemicals and shield their contents from harsh environments like pH uctuations, enzymatic destruction, etc.
Cytotoxic, antibacterial,
antiparasitic, anti- inammatory, antioxidant, and anti- parasitic.
These substances have properties
that inhibit tumour growth, reduce oxidative stress, prevent the formation of amyloid plaques, inhibit the aggregation of platelets, and reduce inammation.
Surface functionalization
of carbon nanotubes with polymers and other biocompatible materials has signicantly enhanced their inherent characteristics. Furthermore, the shape of carbon nanotubes enables them to be combined with other nanosystems and used to create hybrid nanocarriers. These nanocarriers may then be used as theranostic modalities for the diagnosis and treatment of cancer and other diseases.
(Youse
etal.,
2020)
(Thapa etal.,
2013)
(Thapa etal.,
2013)
(Jogi etal.,
2018; Saliev,
2019)
nanoparticles have since their surface area allows them to hold an amount of medication. This is particularly useful for delivering remedies that require concentrations for their medicinal effects. Again, the controlled release of drugs enclosed in nanoparticles can be adjusted thus facilitating delivery (Wahab etal., 2022). For instance, when administered orally, curcumin – an extract from turmeric – is poorly water- soluble and bioavailable. Scientists have discovered that by delivering the drug encapsulated in nanoparticles, its solubility can be enhanced while avoiding metabolic degra­dation of curcumins, the efcacy of the drug with respect to diseases like cancer and inammatory disorders increased hundredfold (Sohn etal., 2021).
218 Herbal Pharmacopeia

10.2.2 NaNocapsules

In these systems, the drug is tightly caged in a core which is, in turn, covered by a membrane. This protective construct shields ingredients from degradation. It can allow for the controlled release of contents. Treatment including nanocapsules brings benets associated with better drug stabil­ity, targeted distribution, and reduced side effects (Kaur etal., 2022). To transfer the substance to the desired location of action, the nanocapsules associate the release of their payload with signals such as pH uctuation or the presence of an enzyme. Essential oils, for example, can be success­fully contained within nanocapsules, despite their susceptibility to oxidation and disintegration. Within such encapsulation, their therapeutic capabilities are kept, and a controlled release is made possible; therefore, they exhibit improved multifunctionality in the treatment of numerous ailments (Elmarzugi etal., 2023).

10.2.3 NaNospheres

The drug, in nanosphere matrix systems, is uniformly distributed over the polymer matrix. Herbal ingredients, for which these solid colloidal particles are particularly useful, lead to a sustained thera­peutic effect from the long- term, steady release. Nanosphere herbal medicines enhance the bioavail­ability and raise resistance to the environmental deterioration of poorly soluble herbal chemicals. Additionally, there is a possible creation of nanospheres programmed to release their content in a controlled manner, which enhances the overall effect of the herbal medicine (Dubey etal., 2022). For example, the alkaloid berberine, which has numerous therapeutic benets, has low solubility and quick metabolism, reducing its bioavailability. These studies have revealed that berberine has great therapeutic potential in the treatment of cardiovascular and diabetes problems. Berberine is encapsulated in nanospheres, which slows the release of the substance and boosts bioavailability (Mirhadi etal., 2018).

10.2.4 NaNotubes

A nanotube is a cylindrical nanostructure with special thermal, electrical, and mechanical character­istics. Nanotubes have the potential to serve as vehicles for medication delivery in herbal medicine by bypassing biological barriers and covering huge surface areas. Carbon nanotubes (CNTs) have sparked widespread interest due to their possible applications in medicine administration. Their vast surface area allows for effective medication loading, while the capacity to pass through bio­membranes allows them to carry herbal ingredients to some specied cells or tissues, if needed. The herbal medicine nanotubes increase the bioavailability and efciency of some herbal constituents (Waris etal., 2022). For instance, resveratrol has been studied in transport mechanisms as it is asso­ciated with CNTs for its anti- inammatory and antioxidant properties. The loading of the polyphe­nol compound into carbon nanotubes increases the solubility and bioavailability of resveratrol by many folds, thereby increasing its therapeutic effect properly (Kale, 2023).

10.3 INNOVATIVE APPLICATIONS OF NANOTECHNOLOGY

Nanoscience, which investigates structures and materials at the nanoscale, has brought about sig­nicant advancements in other disciplines via its creative implementations. Table 10.2 is a compre­hensive summary of some crucial domains in which nanoscience is exerting a substantial inuence.

10.3.1 aNti- caNcer herbal NaNomediciNe

It has been specically shown that nanotechnology increases the potential of herbal medicines in treating cancer. Figure 10.2 illustrates how herbal nanomedicines for cancer treatment use
TABLE 10.2 Key Attributes and Outcomes of Nano Formulation with Pharmaceutically Active Compounds
Pharmaceutical
Biological Activity Nano Formulation
Anti- inammatory activity
Anticancer Magnetic Nanoparticles Curcumin and temozolomide A dual drug delivery system combining curcumin and temozolomide is
Antidiabetic Activity Polymeric Nanoparticles Syzygium cumini extract A big improvement in the long- term problems caused by diabetes mellitus. (Bitencourt etal.,
Nanocarrier transdermalgel Diclofenac diethylamine
Nanotransfersomes Diclofenac, diethylamine,
Gold metallic nanoparticles Quercetin Enhance Bioavailability (Ozdal etal., 2019)
Nanoemulsion Capsaicin Reduce adverse effects while boosting anti- inammatory properties (Ghiasi etal., 2019)
PLGA/ Polymeric
nanoparticles
Silver Nanoparticles Bauhinia tomentosa Linn
Liposome based gold
nanoparticles
Zein nanocapsules Resveratrol An increase in efcacy was seen for Ehrlich ascites mammary tumors. (Elzoghby etal., 2017)
Albumin nanoparticles Berberine Effects that work together in lung cancer better internalization of cells higher
Zinc oxide nanoparticles Hibiscus subdariffa leaf
Liposomes Sterols Blood sugar level drops by 50%. More insulin can pass through Caco- 2
Gold nanoparticles Guavanoic acid In vivo tests show that it has effective anti- diabetic ability in L6 rat skeletal
Active Compound Major Outcomes References
Enhanced biological activity. Targeted medication delivery (Chaudhary etal.,
with curcumin.
High bioavailability. (Chaudhary etal.,
and curcumin
Capsaicin persistent impact and focus on the action's site (Baskaran etal., 2017)
enhancing anticancer activity through stimulation.
Effective drug for treating cancer (Mukundan etal.,
Leaves extract
Curcumin When tried on the B16 F10 (melanoma) cell type, it caused damage to cells
that could not be xed.
levels of caspase- 3 and lower levels of VEGF.
Makes Th1 and Th2 cells work more
extract
Increases the production of insulin receptors Mice’s blood sugar levels were raised again after they were given.
monolayers.
muscle cell lines.Better intake of glucose that depends on insulin
2014)
2013)
(Dilnawaz & Sahoo,
2013)
2015)
(Nakamura etal.,
2014)
(Elgohary etal., 2018)
2016)
(Bala etal., 2015)
(Cui etal., 2015)
(Govindaraju &
Suganya, 2020)
(Continued)
Applications of Nanotechnology in Herbal Pharmacology 219
TABLE 10.2 (CONTINUED) Key Attributes and Outcomes of Nano Formulation with Pharmaceutically Active Compounds
Pharmaceutical
Biological Activity Nano Formulation
Antifungal Activity Liposome Garlic extract Liposomes made with garlic extract showed a promise as an organic method
Micelles Capsofungin +
Solid lipid Nanoparticles Terbinane hydrochloride The gel formulation of Terbinane hydrochloride, which utilizes solid lipid
Silver, titanium dioxide,
cobalt (II) hydroxide and cobalt (II,III) oxide nanomaterials
Antibacterial
Nanosuspension Zerumbone Formulations including particles with a size of 200 nm were created, resulting
Nanoemulsion Curcumin The formulation had a droplet size of 196 nm, resulting in a signicant
Solid lipid nanoparticles Triptolide The lymphatic system efciently absorbed SLN containing triptolide, resulting
Silver nanoparticles Cinnamon, clove The silver nanoparticles produced from cinnamon and clove displayed bigger
Liposomes Garlic oil SLN were manufactured with an entrapment effectiveness of more than 90%.
Microspheres Camptothecin Camptothecin is pH sensitive inside the human body. Encapsulation in
Active Compound Major Outcomes References
to kill fungi in baked products.
Small drugs that do not dissolve in micelles should be used more often to treat
Amphotericin B
Spirulina platensis extract The silver nanoparticles and cobalt (II) hydroxide nanomaterials exhibited
diseases in the gall bladder or bile duct.
nanoparticles, exhibited superior efcacy against Candida albicans in comparison to the commercially available traditional preparation.
potent antifungal activity at a concentration of 50 μg/mL, as shown by their minimum inhibitory concentration (MIC) values.
in a substantial (p < 0.05) increase in both the saturation solubility and dissolving rate by a factor of 2.
improvement in solubility by up to 95% and an eight- fold increase in bioavailability.
in little damage to the liver and kidneys. Enhanced anti- inammatory efcacy was reported because of increased oral bioavailability and sustained plasma drug levels.
regions of inhibition (10 mm) than amoxycillin (8 mm), indicating a high level of antibacterial efcacy.
The formulation considerably increased garlic oil solubility, as evidenced by drug release studies in a phosphate- buffered media (11% in 17 hours).
PLGA microspheres provided stability in an acidic microenvironment. The microspheres (1.3 μm) had a stronger anticancer effect when cancer cells absorbed them more effectively.
220 Herbal Pharmacopeia
(Pinilla etal., 2019)
(Hsieh etal., 2017)
(S etal., 2014)
(Sidorowicz etal.,
2022)
(Md etal., 2018)
(Onoue etal., 2010)
(MEI etal., 2005)
(Cinthura & Rajaseka,
2020)
(Wencui etal., 2015)
(Tong etal., 2003)
Applications of Nanotechnology in Herbal Pharmacology 221
FIGURE 10.2 Herbal Nanotherapeutics: Applications and diverse nanomaterials employed in nanomedicine.
nanocarriers to improve the absorption and accurate distribution of medicinal herbal elements. Nanocarriers, such as nanoparticles, liposomes, and dendrimers, might overcome the limitations of traditional herbal therapy by shielding active components from degradation, increasing absorption, and ensuring effective targeting of cancer cells (Hare etal., 2017). All preclinical studies using in vitro and in vivo models of breast cancers grown on mice have demonstrated the efcacy of Nano Swarna Bhasma (NSB). Ayurvedic drug containing gold nanoparticles is a highly potent agent in preventing the growth of breast tumors. During the animal trials, the NSB medication showed great efciency in regulating the growth. The NSB drug effectively inhibited tumor growth in untreated controls at doses Sof 3–7 mg per 30 kg mouse, twice a week. The ndings provide strong empiri­cal evidence supporting the therapeutic use of NSB medicine in humans for the treatment of breast cancer, as well as the potential to increase patient duration and quality of life (Khoobchandani etal., 2020). Researchers found that AgNPs derived from Dimocarpus longan leaf extract effec­tively inhibited non- small cell lung cancer (NSCLC) cell proliferation in H1299 lines by targeting NFκB, bcl- 2, caspase- 3, and survivin. The AgNPs signicantly suppressed tumor formation when administered to SCID mice using the mouse H1299 xenograft tumor model. AgNPs are effective
222 Herbal Pharmacopeia
alternatives to chemotherapy for preventing lung cancer in living creatures. Further study is needed to assess the possible use of AgNPs in NSCLC therapy (He et al., 2016). In vitro investigations on the MCF- 7 tumor cell line exposed that nanocapsules with no load (CNs) had extremely low levels of cell toxicity, indicating good biocompatibility. The cytotoxicity of 5-uorouracil- loaded nanocapsules (CN- 5-FU) was shown to be dosage- dependent, with cell viability reduced by up to
61.33%. However, the cytotoxic effect of hybrid nano capsules (CNM- 4-5-FU) was less potent. The uncharged treatment for the 5-uorouracil therapy resulted in a cell viability of 58.24% at maximum dose. The outcomes reveled that nanocapsules loaded with 5-uorouracil efciently decreased the cell viability in a controlled manner (Dellali etal., 2020). The MTT test was used to examine and compare the cytotoxicity of curcumin- loaded chitosan/multiwalled carbon nanotube nanocompos­ite on breast cancer cells and normal retina cells. Cell viability is measured 48 hours after adding curcumin, Cs, or Cs/MWCNT nanocomposite (0.78–100 μg/ml). The results show that there would be a larger reduction in the percentage of viable MCF- 7 cancer cells compared to normal cells RPE1, indicating that cytotoxicity against cancer cells is stronger. These ndings thus indicate that curcumin can be efciently loaded within a Cs or a Cs/MWCNT nanocomposite for the purpose of preferentially killing malignant MCF- 7 cells in comparison to non- cancerous RPE1 cells (Sobh etal., 2019). Herbal nanomedicine shows potential as a new approach in cancer treatment. Close interdisciplinary collaboration of experts in herbal medicine and nanotechnologists could pull out strategies to create much safer and more effective therapies. The bottom line is that, although nano­medicine confers good opportunities, each case needs correct guidance and specic recommenda­tions by health professionals in relation to time- tested advice and proper treatment choices.

10.3.2 aNti- iNflammatory herbal NaNomediciNe

Inammation is a defence mechanism among higher species, occurring as a response to hazardous stimuli such as microbial infection, tissue injury, and other types of tissue damage. Inammation is a necessary aspect of the host’s immunological response to tissue damage or infection. If the physi­ological response involved in inammation were absent, then the rate of wound healing would be faster, with fatal results from infections. During the inammatory response, healing processes begin to replace damaged tissue and ll in the gaps with brous tissue. As shown in Figure 10.2, nanotech­nology is just one of the new technologies being used to numerous industries in a variety of ways. Different nanotechnological pharmaceutical formulations have been created to deliver medications to damaged organs while reducing unwanted drug effects caused by medication accumulation in areas not targeted (Abo- Zeid et al., 2021). Kedi etal. (2018) found that oral treatment of silver nanoparticles signicantly reduced carrageenan- induced paw edema in rats. Reduction rates cor­responded to the three dosages in the 1h group, which were at a 44.30% reduction, the 5h group at a
57.60% reduction, and the 5h group at a 60.50% reduction. These were inferior to the effects of the classic medicine, indomethacin, with an inhibition of 71.50% obtained at the 5th hour (Kedi etal.,
2018). Silver nanoparticles (AgNPs) were tested for anti- inammatory efcacy using an in vivo experimental model of inammation generated by carrageenan in Wistar rats. The Animal Ethics Board at the ‘Iuliu Hat ieganu’ University approved this work. The rats were kept in good condition before being divided into four groups: two experimental groups, one treated with the extract from the leaves of Viburnum opulus, one treated with AgNPs, and a positive control and a negative control that received NaCl treatment. Induction of inammation was implemented by injecting the right hind footpads of the rats with carrageenan. The measurements for the edema of the rat paws were done in the paw at 2, 24, and 48 hours following the induction of inammations (Moldovan etal.,
2017). Levels of the cytokines in the soft paw tissues were accessed using the multiplex cytokine kit to assess inammation. Units of various therapies used to render paw oedema using rats that had induced arthritis. On th rst day after Complete Freund’s adjuvant (CFA) administration, edema was found, and the control groups, which were only treated with saline, continued to show this characteristic. Resveratrol, curcumin, and combined solution administration revealed no statistically
Applications of Nanotechnology in Herbal Pharmacology 223
signicant decrease in edema with respect to the control. Nanoencapsulation favored anti- edema action of polyphenols. Nanoencapsulated curcumin (C- LNC) signicantly reduced edema on the 16th and 22nd days whereas Coencapsulated polyphenols (R- LNC) showed signicant from days 15 to 20 and on day 22. RC- LNC reduced edema during days 16 to 22 with percentage reductions of 37–55%, which is very superior when compared with the solution form control (Coradini etal.,
2015). Sulaiman etal. (2020) conrmed the biocompatibility of Hsp- AuNPs, which is validated by earlier research of comparable nanoparticle systems, indicating that they are safe to use in an organ­ism. With the global population increasingly resorting to medicinal plants to manage inamma­tion, this discovery will pave the way for the development of new pharmaceuticals that harness the therapeutic benets of Hsp and its active variants (Sulaiman etal., 2020). Many plants were tested for anti- inammatory properties, but only a few have advanced to clinical studies. This is primar­ily due to issues with the bioavailability and solubility of herbal extracts/rutin. Nanoparticles have been utilized as herbal medication carriers, as the objective has been to improve the generally poor bioavailability of herbals or to improve tissue distribution of treatments. It also provides prolonged release while protecting the pharmaceuticals from physicochemical degradation.

10.3.3 aNtibacterial herbal NaNomediciNe

Since ancient times, organic goods and remedial plants have played an important part in provid­ing healthcare and promoting the health of various populations. Natural origin pharmaceuticals, such as medical plants, herbal remedies, animals, fungi, and marine creatures, account for approxi­mately 50% of the market. Some of the biologically active compounds present in extracts show poor absorption and dispersion, as depicted in Figure 10.2. This may lead to reduced bioavailability and efciency, thereby limiting their practical applications in a clinical setting. Most herbal com­positions consist of biologically active ingredients with poor absorption and poor dispersion that together reduce their bioavailability and effectiveness. This can prevent applicability in the clinical setting. Several nanomedical techniques have been proposed to address this issue. These proposed techniques include polymeric nanoparticles, solid lipid nanoparticles, liposomes, microemulsions, liquid crystals, and transparent precursor carriers for liquid crystals. Overall, various sorts of nano­technological systems, applied alone or in combination, enhance the potential of herbal prescription formulations for greater absorption and biological action (Ahmed etal., 2021). An experiment was planned to investigate the potential for wound healing following treatment with silver nanopar­ticles derived from two major medicinal plants, Catharanthus roseus and Azadirachta indica leaves, on female BALB/c mice in an excision wound model. Both C Ag NPs and A Ag NPs demon­strated greater wound constriction than the control and positive- control groups. C AgNPs healed the wound by 94%, while A AgNPs closed it by 87%, as opposed to silica gel, which only closed it by 74%. This faster- healing tendency was attributed to the antibacterial character, which prevented the bacterium from causing irritation and inammation. Overall, C Ag NPs displayed greater heal­ing than A Ag NPs (Lakkim etal., 2020). The study examined by Attallah etal. (2022) measured macroscopic wound healing rates for the various groups on days 0, 3, and 7. The treatments used either Betadine™ or Gardenia thailandica Tirveng silver nanoparticles (AgNPs). By the end of the experiment, leaves treated with either of the two treatments had completely healed compared to the control. By the third day, the Betadine and AgNPs groups had signicantly healed their wounds, at
90.19% and 92.3%, respectively, compared to the control group. On the seventh day, the Betadine™ and AgNP groups saw nearly complete healing, with rates of 99.02% and 99.23%, respectively, compared to the control group. Furthermore, colony- forming units per milliliter (CFU/mL), as the count for the presence of bacteria, reects a signicant decrease in both groups compared with the control group. Results also reveal the fact that Betadine and AgNPs have drastically affected the healing process of the wound and have synergistically reduced the bio- burden (Attallah etal.,
2022). In a S. aureus burn infection model, the combination of multi- walled carbon nanotube ten­sor and levooxacin (MWCNT- LVX) was found to kill or limit bacterial growth. At doses of 312.5,