Environmentally friendly technologies use less energy and produce less carbon dioxide due to lower temperatures and chemical conditions. Supercritical CO2 degumming reduces water and energy use and recovers and reuses CO2, an advanced recycling capacity not seen in previous approaches. This technique produces sericin, a clean and possibly lucrative byproduct that can be used in other industries, fostering a circular economy (Numata and Kaplan softer processing conditions, green silk fibroin has improved biocompatibility and mechanical integrity. This is important for medical applications since natural protein structures improve performance and minimize immune reactivity when implanted (Kundu et al. 2013a). Transitioning to green synthesis processes is difficult despite the benefits. Due to specialized equipment and pricier materials, setup and operation expenses may be greater. Some green procedures are difficult to scale, especially for medical industry output. However, the long-term benefits—reduced environmental impact, increased worker safety, and possibly lower regulatory and cleaning costs— make further research and adoption worthwhile. Green chemistry will likely improve efficiency and cost-effectiveness to increase industrial applicability. Traditional methods are optimized for yield and cost-efficiency, but they lack sustainability. Green silk fibroin synthesis addresses environmental and health concerns and improves product quality and safety. As the world prioritizes green production, the silk sector can lead by example, showing that environmental responsibility can coexist with technological and industrial growth (Unger et al. 2004).

RSF (regenerated silk fibroin) scaffolds are a preferred biopolymer for use in ligament and tendon TE because of their special mechanical qualities (such as high toughness values and good elasticity) and structural integrity (Kundu et al.