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16.6.3 Use asaPreservative
Thyme oil is used as a botanical preservative to stop the deterioration of food during
storage. Because of its antifungal and aatoxigenic properties, Thymus oil is used as
a preservative for storing food items. Research reported that thyme oil completely
inhibited the growth of fungus Aspergillus avus [47]. Thyme oil completely arrests
the production of aatoxin B1. It is also used to control postharvest fungal diseases.
Usage of thyme oil increases the shelf life of the cake. It is also reported to reduce
the decaying of vegetables.
16.6.4 Aromatherapy
A pleasant odor has always been desirable and it still makes people feel good. In
ancient civilizations, similar to aromatherapy fumigation was carried out to treat
patients. It was an effective treatment as using it disinfected the air and the sweet
smell induced calmness in patients. Until nineteenth century good smelling candles
were ignited by people to make the air clean. Even various essential oils were being
sniffed for protection against infections.
In aromatherapy, different thyme oils are used. Thymol, red thyme oil. Linalool
are used due to their soft texture and antibacterial activities. White thyme oil is used
too. It has a slighter effect on the skin. It is also used to cure ringworm, athletes’ foot
and fungal infections.
16.6.5 Use asGarden Plants
Several Thymus species are used as ornamental and garden plants. A huge trade of
thyme plants is carried between North America and Europe. They are being traded
for their decorative foliage and aromatic properties.
16.6.6 Food Applications
Since early times, herbs have been utilized for improving the look and taste of
food. Usage of thyme in food industry is restricted to preservation of meat products. It acts as an antioxidant and a preservative. Literature demonstrates that
addition of thyme natural antioxidants can greatly reduce rancidity in different
types of food. Thereby, different researches have been conducted to prove the
preservative properties of thyme. It has been found that bioactivity of compounds
and their preservative properties in foods can be greatly inuenced by the addition of thyme. There are several implications of addition of thyme to food but

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these can be avoided by different techniques. The primary goal of using thyme in
food is to enhance its shelf life however, there is one limiting factor. The usage of
thyme plant extract or its essential oil develops negative organoleptic characteristics in food leading to undesiring odor and taste. These sensory limitations can be
avoided by using low concentrations of thyme and other conservation methods.
Moreover, nanocarriers’ encapsulated natural compounds can also be used for
this purpose. This encapsulation not only masks taste but also protects against the
oxidative degradation by controlled release of compounds. It also increases the
bioactivity of compounds present in thyme essential oil and its plant extract.
Thyme essential oil and extracts are also used for preserving sh and seafood. For
this purpose, the sh or seafood is immersed in essential oils or natural extract
followed by drainage of product after half an hour. EO can also be used by applying on the lms or adding it to the surface of sh. Due to short shelf life, fresh
thyme is not used as food. Use of whole dried thyme in food processing is also
limited. It is because the volatile compounds are not evenly distributed throughout the product and often additional treatments are required for processing of
food. One such method of food processing is comminution. In this method leaves
of thyme can be crushed, ground, chopped or sliced. When ground the oil structure becomes crushed and the oil is released. However during grinding heat is
generated that vaporizes the volatile oil as a result avor of oil reduces. To prevent destruction of volatile compounds by heat it is essential to keep the temperature low during the grinding process. Moreover, ground thyme should have
optimum moisture to retain the characteristic odor and avor. To prevent contamination of thyme by microbes in processed food, it is sterilized through different
chemical and physical sterilization techniques.
T. Iftikhar etal.
16.6.7 Usage inPerfumery
Because thyme has a capacity to suppress smells it is used in deodorants.
Moreover, it also acts as an antimicrobial agent so have no harm in using in cosmetics. Its essential oil is also used in soap perfumes. The oil greatly masks the
tarry odor. Usage of thyme EO in trace amounts in lotions, perfumes or colognes
provides freshness to body. Names of some perfumes containing thyme essential
oil or its plant extract are Milano Fraganze Basilica and Acqua Colonia Lavender
& Thyme.
16.6.8 Culinary Uses
In Mediterranean region, thyme is widely used for seasoning. Its strong agreeable
aroma is highly recommended for seasoning of food products like pizzas etc. It also
gives a pleasant touch to fatty foods like sausages, fatty meats and strong cheeses.

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Moreover, it can also be used in soups, meat and sh dishes. Fresh leaves of thyme
are also used in salads. Thyme is also used in liquor industry to give avor and
aroma to wine.
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16.6.9 Pest’s Control
Thyme essential oil has been effective against a number of pests attacking various
plants. Every year in United States billions of dollars are lost due to vegetables and
fruits losses during harvest and distribution process. Mostly these post-harvest
losses are due to mishandling and aws in storage procedures and diseases. Botrytis
cinerea commonly known as gray mold fungus attacks almost 200 different plant
species. It is also major pest that attacks strawberry (Fragaria ananassa) infecting
its fresh fruit thus causing major crop loss. Botrytis cinerea reduces the shelf life of
strawberries. In a recent research conducted it has been found that using thyme
essential oil on post-harvest strawberries can signicantly mitigate the impact of
this fungus [48].
Thyme EO can also be used to reduce the incidence of bacterial blight in soybean
plants caused by Pseudomonas savastanoi. Usually this pathogen is controlled by
using toxic pesticides that causes pathogen resistance in plants. Using eco-friendly
essential oils is benecial option. Research conducted showed that using thyme
essential oil in seed treatment greatly reduced the incidence and severity of this
disease [49]. Essential oil of thyme is also benecial in poultry industry. New Castle
Virus (NDV) is the most deadly viral disease in poultry industry. Even by using vaccines this disease has not been controlled. As Thymus vulgaris L. contains variety of
volatile and lipophilic properties such as monoterpenes, sesquiterpenes and phenylpropanoids, it has been proved effective in controlling this disease. These substances
can act against different microorganisms such as fungi, yeast, bacteria and viruses.
Thyme essential oil attacks envelope of New Castle Virus and its viral components
that are necessary for attachment and penetration in host cells [50].
Maize is widely cultivated crop throughout the planet. Solely in China around 11
billion tons of corn has been utilized in year 2021–22. Due to its high nutrient content maize is loved globally. Its high demand in world has led to the development of
techniques to prevent maize losses in elds and in warehouses. Sitophilus zeamais,
is one of the most lethal pests for the stored grains. Particularly found in tropical
regions and warm zones, this pest is the major destroyer of maize crop. It severely
damages maize during storage. Using articial pesticides have harmful impacts on
consumers. Therefore, continuous researches are being conducted to nd ecofriendly alternatives for this pest control. Application of thyme oil on this pests has
found to signicantly reduce its mortality. Research has shown that both encapsulated and non-encapsulated thyme essential oil is effective in controlling Sitophilus
zeamais without altering the quality of crop [51].
Essential oil of Thymus vulgaris L. is also reported to control early blight disease
in tomatoes. This disease causes severe economic losses by damaging the plants.

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Infection by this pathogen starts at the bottom of plants and spreads upward thus
turning the leaves yellow and creating spots upon them. It was found that thyme
essential oil greatly inhibited the mycelial growth and conidial germination in
tomato plants. Moreover, spraying tomato plants with thyme essential oil enhances
their defense mechanism by increasing the production of defense related enzymes
like peroxidase and polyphenol oxidase. Thyme EO is also reported to boost callose
deposition within tomato plants infected with this fungus [52].
T. Iftikhar etal.
16.6.10 Other Uses
Thyme is effective in repelling cabbage y, white y and aphids. Moth can also be
repelled by using thyme. For this purpose, take a muslin ribbon and ll it with dried
thyme. Placing these bags within the folds of blanket and cloths and keeping them
in suit cases or drawers will keep the moths away. For ornamental purposes only
few species of thyme are used. It is best for garden’s rock. It can also be grown as
border plants. Thyme is also used in making butter, bread, thyme vinegar, cocktails
and other condiments.
The essential oils of Thymus vulgaris L. and Thymus serpyllum works against
Anathoscelides obtectus, the pest that damages Phaseolus vulgaris L. (kidney bean)
in eld and during its storage. Thyme essential oil have toxic effect on insects and
stops their reproduction through ovicidal and larvicidal effects. Essential oil of
Thymus vulgaris L. also inhibits the reproduction of mite Tetranychus urticae. The
compound thymol present in thyme oil greatly reduces the egg laying capacity of
this mite. It also possess genotoxic activity. Such effects have been visualized in a
study conducted on somatic mutation and recombination test on Drosophila. Other
compounds constituting thyme essential oil are found ineffective with regard to
insecticidal activity. Another pest sensitive to Thymus vulgaris L. essential oil is
Spodoptera littoralis. If the larvae of this pest are fed with leaves treated with T. vul-
garis essential oil it reduces their development and egg laying capacity. Thymol is
also extremely toxic against housey. Researches have shown that aromatic plants
exhibit dual insecticidal activities i.e. causing direct toxicity on adult insects and
inhibiting their reproduction.
16.6.11 Thyme Honey
Thyme honey produced by different thyme species the most prominent among
them is garden thyme (Thymus vulgaris) and wild thyme (Thymus capitatus).
Thyme honey is renowned worldwide for its unique avor and aroma. It is being
produced from the mid of June till the rst half of July. Color of thyme honey
varies from light amber to brown. In liquid state its color varies from light amber

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to amber whereas in crystallized form it ranges from beige to brown color. It has
strong aroma and a taste similar to tropical fruits. It is rich in avonoids, phenols
and avanols. Often it also contains epicatechin gallate. Thyme honey, if kept
outside, crystallizes within short time.
Thyme honey was traditionally used in Greece. It is widely found in Greece.
Famous source of thyme honey in Greece is slopes of Mount Hymettus near
Athens. A popular way of eating thyme honey there is by eating it with fresh
Greek yogurt and nuts. In Sicily thyme honey is extracted from wild thyme
(T. capitatus). There it is known as Hyblaean Honey. In Italy and New Zealand
thyme honey is quite popular. It was introduced in New Zealand by gold miners
back in 1800’s. Thyme plant became popular in New Zealand countryside Central
Otago as a source of delicious thyme honey. Every year in Central Otago
Alexandra Thyme Festival is celebrated by the tourists and natives to celebrate
the beauty of landscape when the wild thyme blooms and also the ne honey
produced from it.
Thyme honey due to its useful ingredients is being employed in number of treatments. Several researches have been conducted to explore its miraculous properties.
Thyme honey is found effective in treating oral mucositis. This oral mucositis is one
of main side effect of head and neck treatment of cancer patients. Using thyme
honey has found to improve the quality of life in patients suffering from head and
neck cancer [53]. Thyme honey nasal spray is also found to have positive effects on
patients suffering from chronic rhinosinusitis after their endoscopic sinus surgery.
This nasal spray constituting thyme honey signicantly reduces inammation,
polyp formation and also boosts the healing of mucosal lining within the patients
suffering from chronic rhinosinusitis. However, further research is required in this
regard [54].
Traditionally honey has been used as a remedy for wound treatment. Several
clinical trials have been carried out to obtain super normal wound healing. To
achieve this goal research has been conducted upon the efcacy of thyme honey
in wound treatment. It has been found that if applied twice a day on wounds,
thyme honey accelerates the healing process. Thyme honey also helps in providing relief from cough. Moreover, its viscous sticky nature soothes the throat.
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16.7 Medicinal Uses ofOther Species ofGenus Thymus [55]
16.7.1 Thymus pubescens
This species is widely found in Iran and Turkey. It is a perennial plant with a woody
stem and red or purple owers. Flowering begins in spring and continues till summer. It is used as a food additive and for treating gastrointestinal disorders
(Table16.3).

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T. Iftikhar etal.
Table 16.3
Property Characteristic/value
Molecular
Formula:
Molecular
Weight:
Components: 0.8–2.6% of thyme oil; Thyme oil from T. vulgaris contains 3–80% Thymol
Form at 25°C/1
Atm.
Color colorless to reddish brown
Odor Pleasant smell
Specic Gravity 0.916g/cm
MP 250°C
BP 190°C
Solubility Only slightly soluble in water; soluble in alcohol (80%), Thymol is soluble
VP 0.167mm Hg at 25°C
pH Thymol: Solution neutral to litmus test
Water coefcient 3.3 (Log K
Miscibility Miscible in water
Flammability At 60°C it is ammable
Storage stability Stable if stored under optimum conditions
Corrosion
characteristics
Physicochemical properties of Thyme [27]
C
50H82O4
747.18368g/mol
carvacrol; linalool, p-cymene, γ-terpinene, borneol, 1, 8-cineole, geraniol,
various other terpenoids, alcohols and esters.
liquid in state
3
in glacial acetic acids, oils and xed alkali hydroxides
: −1.77 to −1.69)
ow
Corrosive
16.7.2 Thymus caramanicus
It is also a woody plant, about 25–50cm tall. This plant shows high antibacterial
activity. Leaves of Thymus caramanicus are used in rheumatism treatment, allergy
and dermal problems. It is also a natural food preservative. Major constituents of
this plant are thymol, borneol and p-cymene.
16.7.3 Thymus kotschyanus
It is a perennial plant about 20cm tall. It has dark green pointed leaves. Leaves of
this species are used in spices and medicines. This species is mostly found in the
Mediterranean region. It also grows at high altitudes in mountains. It contains
14.9% thymol. Altitude greatly affects the chemical composition of the plant.

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16.7.4 Thymus transcaspicus
It is also known as Khorasan thyme. As the name indicates it is native to Iran. This
species is also found in Turkmenistan. It is an aromatic plant containing thymol and
carvacrol as the main constituents. It contains 60% thymol whereas around 90% of
monoterpenes are also reported. This species is reported to have high antifungal
activity.
16.7.5 Thymus persicus
This species is found in the West Azerbaijan province. It is also endemic to Iran.
This species is characterized by small leaves and by the presence of glandular hair.
Important constituents of this plant are thymol, sabinene hydrate, carvacrol, borneol, geraniol.
16.7.6 Thymus fallax Fisch. & C.A.Mey
It is a pleasant-smelling shrub that is found in Western Mediterranean, Southern
Italy, Iran, and Turkey. It grows on rocks and grassy plains. This species is used in
making soups, herbal tea for treating seasonal u. Its leaves extract and oil is also
used in pesticides, mouthwashes, fumigants etc.
16.7.7 Thymus algeriensis
It is a North African species widely grown in Tunisia, Morroco and Algeria. Both
fresh and dried leaves of Thymus algeriensis are used as kitchen herbs. It is used for
curing respiratory diseases and issues of digestive tracts. Researches indicate that it
is also used as anti-abortive.
16.7.8 Thymus serpyllum
It is grown locally in Mediterranean Europe and North Africa. T. serpyllum is commonly known as mother of thyme. It is mostly used in home remedies. It is used as
an antiseptic and stimulant in mouthwashes. It contains various compounds that
have high resistance against infections. It is also used for treating ailments.

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T. Iftikhar etal.
Table 16.4
uses of Thymus sp. [55]
Ethnobotanical
Thymus species Treatment of diseases
Thymus pubescens Gastrointestinal disorders
Thymus caramanicus Rheumatism
Thymus kotschyanus Depression and cancer
Thymus persicus Toothache reliever
Thymus fallax Seasonal u
Thymus algeriensis Respiratory and digestive issues
Thymus serpyllum Treat infections
Thymus transcaspicus Fungal diseases
Thymus citriodorus Bacterial disease
16.7.9 Thymus citriodorus
It is commonly known as lemon thyme. It is smaller in size than Thymus vulgaris
L. T. citriodorus has deep pink owers and dark green leaves. Its leaves have lemon
smell. Out of the total 50 known species of this thyme, only 3 are cultivated for
cooking purposes. It helps in curing bacterial diseases (Table16.4).
16.7.10 Myths, Legends, Folklore andTales About Thymus
vulgaris L.
The word thyme is derivative of the Greek word ‘Thymos’ which means to fumigate. Studies showed that it was utilized by ancient Egyptians in the embalming
process. Since ancient times thyme has been considered a symbol of bravery. Roman
soldiers used to bathe in thyme and they believed that it gives them courage. Until
medieval times it was given to soldiers upon leaving for battle as it was considered
as a sign of valor. The togas of generals were been embroidered with thyme picture.
Greeks knew about thyme from early times. This was the herb the bees loved and
honey produced by bees was loved by Greek Gods. In medieval Europe, when people
had a strong belief in fairies, people used thyme to attract fairies during summer evenings. There are also myths that thyme was present in hay for infant Jesus and since
then it has had a great aroma.
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