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14.32 Diseases Control
A key weakness in high vanilla production in many regions of the world is stem and
root rot disease mainly caused by Fusarium oxysporum. Vanilla crops are badly
affected under poor management conditions and does not get high degree resistance
against the stem rot. Fusarium is a ubiquitous soil borne fungus, also transmitted by
water splash that also causes rot in many other species. Under severe environmental
conditions, the fungus produces spores i.e., chlamydospores to extend their survival
in the soil. In some conditions, the pathogen may incubate within plant body without showing any prominent symptoms. Because of general poor performance, due
to adverse environmental conditions, of vanilla crop and inuence of various diseases, farmers lose their interest in vanilla production and abandon their farms
within 10 years [58]. Some main diseases of vanilla are Anthracnose and Rust
(Table14.2). Anthracnose is caused by fungus Colletotrichum sp. It attacks fruits,
leaves, stems and owers of vanilla plant. Dark brown sunken spots are the symptoms of this disease. Resultantly, the infected fruit falls off the plant thus causing a
great loss. Sometimes, due to Anthracnose the yield drops by 50%. Fungicides such
as copper oxy-chloride and Mancozeb could be used at a concentration of 2g/L
mixed in distilled water or Bordeaux mixture. Spraying is done immediately after
nortes begin. Moreover, the plants should be well nourished to prevent disease.
Yellow- orange, round pustules on the abaxial surface of leaves are the symptoms of
Rust in vanilla plant. Poor ventilation, excessive shade and high precipitation are the
causes of Rust. Resultantly, the productive capacity of vanilla plant is greatly
reduced. It may entirely defoliate the plant. Copper containing mixtures are used to
prevent rust. Infected leaves are removed from vanilla plant and buried. Black rot is
another disease caused by Phytophtora sp. that kills the plant only in few days.
Greenish or blackish coloured watery injuries appear on the infected plant. Damage
starts from the apical part of the plant and then spreads in rest of the body. Poor
irrigation, high rains and excess shade leads to development of this disease. It causes
excessive loss due to fruit loss and rotting [12]. This disease could be prevented by
maintaining proper distance between the plants. Usually, a distance of 2m should
be between plants and 2–2.5m among the rows of plants. Plants should be pruned
regularly so that they could receive maximum sunlight. Infected parts of the plants
should be burnt or removed. Another problem faced in vanilla cultivation is rotting
of recently planted cuttings. This is mostly done by Fusarium oxysporum. In this
infection, rotting of lower portion takes place which then spreads in the upper portion. A white cottony mycelium is observed to be formed at the point of infection.
About 5–50% damage takes place due to this. It could be controlled spraying carbendazim on plants [69].

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Table 14.2 Comparative account of various fungi infecting vanilla crops in various parts of world
Granada & Thailand, India (Kerala) & Thailand [59–61]
Inorescence rot
Sr.
No Casual fungus Disease name Occurrence region
1 Atichia vanillae (Pat.) V.Hach Anthramose Tahiti [27]
2 Calospora vanillae Massae Seychelles, Madagascar, Reunion, Comoro, Tahiti, Antigua, Mauritius, New
3 Colletotrichum gloeosporioides Leaf spot
4 Colletotrichum vanillae & Fusarium oxysporum Leaf rot
South India [61]
Shoot tip rot India (Kerala) [62]
gloeosporioides
5 Fusarium batatis Wollen var. Vanillae Root rot Puerto Rico [22, 25, 47]
6 Fusarium oxysporum & Colletotrichum
Mauritius [27]
Stem rot Indonesia, Ottawa, Bali, north Sulawesi and North Sumatra, India [61, 63, 64]
Rots Columbia, Mauritius & Sri Lanka [27]
Vermicuiaria vanillae Delaer
7 Gloeosporium vanilla (Zimm.) Petch &
8 Macrophomina vanillae Averna Brazil [27]
South India (Kerala) [66, 67]
Stem blight India [66, 67]
14 Phytophthora paimivova Fruit rot French Polynesia [67]
15 Phytophthora parasitica Dast. Tahaa, Raiatea & Huahine [27]
9 Marasmus sp. Horsehair blight [61]
10 Mycoleptodescus indicus Black crust Brazil (Taperoa regions) [65]
11 Pencillium sp., Aspergillus sp., Rhizospus sp. Storage spoilage India & French [47, 61]
12 Phiaiospora vanillae Zimm Java [27]
13 Phytophthora capsici & P. parasitica Bean rot Tahiti, Moorea [66, 67]
16 Sclerotium rolfsii Sclerotium rot India, French Polynesia, China, Uganda [61, 66, 68]

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14.33 Recommendations
There are some recommendations, to check and reduce the severity of disease [70].
Choose a site having abundance of organic matter with no waterlogging. Always
select healthy plants. Make it sure that the wounds are healed, plant is disinfected.
And nally, the gaps between plants must be large enough to avoid denseness.
Manage the light and shade as well as soil organic matter by trimming of tree parts.
Always plant the vanilla after supporting vegetation have been fully established.
Also, remove the diseased vegetation in the area. Rejuvenate the plantation periodically. Moreover, don’t damage the roots of vanilla by stepping in the vicinity. Mulch
having high contents of lignin may stimulate the growth of actinomycetes in the soil
[58, 62].
14.34 Annual Production
Among several vanilla species, Vanilla planifolia Jacks. ex. Andrews has most of
the economic importance as edible crop, i.e., condiment. Most of the developed
countries are working on it while major vanilla growing and exporting countries are
Republic of Madagascar (1681.62 tons), France (>1000 tons), European union
(>1000 tons), Jordan (856.47 tons), United States (637.72 tons), Dominican
Republic (355.46 tons), Turkey (318.01 tons) and Indonesia (287.47 tons)
(Table14.3). The gross annual production of vanilla is approximately 8000tons
with a revenue of US$1121.74 million. United States of America (USA) is the main
consumer (1535.85 tons) followed by European Union (1327.74 tons), United
Kingdom (1014.12tons), France (854.408tons) and Germany (514.55tons). It is
the mostly used fragrance in perfume industry. Moreover, it also has very pleasant
taste owing to the production of aromatic pod, but the vanilla pod doesn’t have characteristics aroma in its green state. This special aroma, due to the presence of a
chemical vanillin, develops as soon as the curing process is done, which may vary
from country to country. Synthetic vanillin is also being prepared from wood
because of very high market demand, although the natural production is superior in
fragrance and avour. Still, the production of synthetic vanillin is assessed at
15,000tons that is almost two times of natural production [72].
14.35 Chemical Constituents ofVanilla
A limited amount of data is available about the chemical composition of mature
green beans of vanilla. Fibers (lignin, cellulose, hemicellulose, etc) make up almost
8% of the fresh weight that is too high because most of vegetables and fruits constitute between 1% and 4%. Sucrose is the most abundant form of carbohydrate and

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T. Iftikhar etal.
Sr. No Country Trade value (Million dollar) Sr. no Country Trade value (Million dollar)
Table 14.3 International trade of vanilla in 2019 [71]
1 Madagascar 651.03 16 Romania 4.10
2 France 89.03 17 Czechia 4.05
3 Indonesia 81.66 18 United Kingdom 3.75
4 Canada 65.81 19 Belgium 2.39
5 Germany 65.77 20 Denmark 2.10
6 Papua New Guinea 37.99 21 South Africa 2.08
7 Uganda 30.15 22 Switzerland 1.77
8 Netherlands 22.34 23 Austria 1.70
9 United States 20.27 24 Australia 1.64
10 Mauritius 18.81 25 Turkey 1.43
11 Poland 13.22 26 Thailand 1.26
12 India 11.43 27 Italy 1.13
13 United Arab Emirates 10.09 28 Mexico 1.12
14 Comoros 8.06 29 Jordan 1.07
15 French Polynesia 7.69 30 China 0.90

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total carbohydrates contents are much lesser than present in most of the fruits.
Similarly, the ratio of proteins is much lesser and is comparable with most of the
fruits and vegetables that is <1%. Due to high glucosidase activity, it is indicated
that vanilla contains high level of aromatic compounds. It contains high level of
lipids about 12% of fresh weight. This is too high compared to most of the vegetables and fruits (0.2–0.4%). Most abundant organic acids are malate and citrate
around 80% of all organic acids. Similarly, 470mg and 170mg of potassium and
calcium are present, respectively. These contents are much higher as compared to
their contents in fruits and vegetables. V. planifolia Jacks. ex. Andrews has excep-
tionally high contents of glucovanillin around 1.7% of fresh weight. The main constituent is vanillin 4 hydroxy-3- metoxybenzaldehyde that is almost 80–90% among
all volatiles. The characteristics avour and aroma of vanilla is produced by a series
of enzymatic reactions during curing process. Vanillic acid, anisic acid, anisaldehyde, anisyl alcohol, vitispiranes, caproic acid, phenol ether, phenols, eugenol, carbonyl compounds, lactones, benzyl ethers, B-complex, 15% fats, 25% carbohydrates,
and almost 6% mineral salts like zinc, magnesium, calcium, iron, manganese and
potassium are also reported. Moreover, 35–40% of water contents are also present
in vanilla [29].
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14.36 Vanilla Essence
Vanilla is a chemical extract of beans of orchid species like Vanilla pompona,
Vanilla tahitensis and Vanilla planifolia Jacks. ex. Andrews. The avour of their
extracts shows a noticeable difference. Moreover, the aroma and avour of a same
species vary if grown in different regions of world due to climate and agronomy of
the region. No doubt, it is a labour-intensive product, but it is also among the most
expensive condiment after saffron and cardamon. The pure organic vanilla is rich in
many fragrance and avouring chemicals than synthetic one. Original vanilla is
highly pure extract of vanilla seeds/beans with elegant avour. It is the source of
most complex taste in the world due to the presence of more than 250 organic compounds in it. It is worth noting that the original vanilla extract has dark brown shade
that is characterised by sweet, fruity avour. The synthetic vanilla is being popular
in market due to shortage of organic vanilla because it is unable to meet the demand
of international market. The noticeable achievements are being made to match the
international requirement of organic vanilla [19].
14.37 Medicinal Importance
The usual medicinal usage of Vanilla planifolia Jacks. ex. Andrews has disappeared
with the passage of time irrespective of its economic importance in the world due to
its avour and aromatic characteristics of its fermented pods. According to

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literature, it is being cultivated since centuries in various countries of the world and
once it was utilized to treat more than 12 diseases in sixteenth century. Unfortunately,
currently it is only being used in grave fever and abdomen pain. No doubt, it has
anti-cancer, anti-cell stress, antioxidant, anti-inammatory, anti-microbial, antinociceptive, anti-depressant, anti-cholesterol properties. Therefore, vanilla has huge
potential in modern novel medicine, so its cultivation must be promoted to get maximum benet from this ancient orchid [73]. Vanillin present in vanilla acts as an
active agent in pain relief. It is also responsible for lowering insulin resistance. It is
also used to cure lipid dysregulation [74].
T. Iftikhar etal.
14.38 Commercial Importance
The famous and major international companies use vanilla to enhance the avour of
their products i.e., Pepsi, Coca Cola, Smoothie king, Starbucks, Jones Soda company, Cadbury, etc. Moreover, it is also used in men’s fragrances i.e., Houbigant,
Faberge, New Dance and also in Women’s fragrance i.e., Coty, Lauder, Calvin
Klein, Vera Wang, etc. [72].
14.39 Trade ofVanilla
Vanilla was ranked at 908th most traded product in 2020 across the world having a
total trade of $937M.No doubt, the exports of Vanilla decreased by −17.4%, from
$1.14B to $937M, between 2019 and 2020. Moreover, Vanilla represents 0.0056%
of total world trade [75].
There are two major forms of vanilla being traded in the international market:
• Bourbon vanilla, cured from Vanilla planifolia “Andrews”. This is the most pop-
ular cultivated from/variety in Madagascar. It is worth noting that V. planifolia is
the same variety started and being grown in the Mexico but has become synony-
mous with Madagascar. Bourbon vanilla is the most preferred form of vanilla
used in Europe, although vanilla extracts are also available [75].
• Extract-grade vanilla harvested and cured from Vanilla tahitensis. It has a weaker
vanilla with “fruity, oral, and sweet avours”, which is mostly grown in Papua
New Guinea and Indonesia. Extract-grade vanilla is used to produce vanilla
extract, which is the main form used in the United States of America [75].
In Europe, vanilla is mostly marketed in the following forms:
Ground or Whole natural vanilla normally used as a spice. Vanilla is most traded
in pods (i.e., beans), but vanilla powder can also readily available in the market.
Vanilla extract, used as a avour in the food industry and as a fragrance in the cosmetics industry [75] (Tables 14.4 and 14.5).

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Table 14.4
Sr. No
1 Madagascar 617 68 22
2 France 70 7.8 5.9
3 Germany 43 4.85 3.06
4 Indonesia 39 4.41 4.03
5 Canada 31 3.45 3.35
Table 14.5
Sr. NoName of
1 USA 324 36 0.011
2 France 196 21 0.027
3 Germany 96 10.7 –
4 Canada 70 7.83 0.014
5 Netherlands 56 6.26 –
6 Indonesia 15.8 1.76 –
Export of vanilla of ve major countries
Name of
country
Import of vanilla of six major countries
country
Export amount
(Million dollar)
Import amount
(Million dollar)
Share in world
export of vanilla (%)
Share in world
import of vanilla (%)
Vanilla share in total export of
the country (%)
Vanilla share in total import of
the country (%)
14.40 Conservation ofAnimals Through Vanilla
A major portion of natural habitats are being converted to agroecosystems due to
increasing global demand of food to accommodate crop cultivation. it is worth noting that the agricultural expansion is more prominent in the tropical regions. there
are the areas where huge rural communities are solely dependent on farming income
for their livelihoods. The conversion of Such agricultural land may have severe
impacts on local fauna [76]. Hending etal. (2022) compared the diversity of vertebrate species between natural forest habitat and three types of vanilla plantations
maintained under varying management regimes in northeast Madagascar. They used
diurnal and nocturnal transect method to survey vertebrate diversity. Natural forest
habitat contained the greatest vertebrate species diversity and had proportionally
more threatened and endemic species than all vanilla plantation types. They
observed a greater number of species and a higher inverse Simpson index in minimally managed vanilla plantations located within or near natural forest compared to
intensively managed vanilla plantations. It is worth noting that these ndings are
important and encouraging for animal conservation and sustainable crop cultivation
in Madagascar, and suggest that newly created vanilla plantations, and already
existing plantations, should endeavour to follow the more traditional, minimalistic
management approach to improve sustainability and promote higher faunal diversity [76].

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T. Iftikhar etal.
14.41 Production ofVanilla
There is continuous rise in the harvesting and production of vanilla since 2001. The
rise in production is depicted in Table14.6 (Figs.14.2 and 14.3) [75].
14.42 Biotechnological Applications inVanilla
No doubt, biotechnology has a signicant role on the pattern of development and
quality of life globally [77]. The end of twentieth century observed the bloom in
industries due to discoveries made in the eld of biological sciences and the progress made during recent years in genetic engineering, molecular biology and plant
tissue culture provided a new sight in crop improvement.
In vitro culture is a signicant tool of plant biotechnology, which enables us to
use the totipotent nature of the cells, a concept introduced by Haberlandt (1902) and
rst time practically demonstrated by Steward etal. [78]. It may be used to produce
disease resistant/free clones, conservation of gene pool, mass cloning of desired
genotypes, selection and separation of mutants, boosting of hybrids among sexually
Table 14.6 Vanilla
harvesting and production
since 2001 [75]
Harvested areas
Years
(Hectares)
2000 38,272 3988
2001 39,451 4739
2002 39,867 5309
2003 42,393 4826
2004 42,267 5841
2005 79,455 7499
2006 83,773 9060
2007 85,038 9327
2008 89,643 9302
2009 69,793 9176
2010 86,521 6258
2011 95,252 4602
2012 99,582 8052
2013 96,628 7601
2014 96,402 7080
2015 93,030 7217
2016 93,623 7298
2017 92,840 7180
2018 95,373 7040
2019 91,335 6813
2020 91,585 6881
2021 92,066 6888
Total production of
Vanilla (Tons)

10000
100000
120000
2000
2001
2002
2003
2004
2005
2006
2007
2008
2009
2010
2011
2012
2013
2014
2015
2016
2017
2018
2019
2020
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14 Vanilla
80000
60000
40000
20000
0
Fig. 14.2 Harvesting of Vanilla since 2001
9000
8000
7000
6000
5000
4000
3000
2000
1000
0
000102030405060708091011121314151617181920
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Fig. 14.3 Production of Vanilla since 2001
incompatible taxa by somatic hybridization, introduction of selected traits by
genetic engineering, and in the mass production of secondary metabolites using
cultured clones, i.e., cells or tissues [15].

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T. Iftikhar etal.
14.43 Problems toBeAddressed
As a cash crop, vanilla is playing critical role in the economy of Comoros, Indonesia,
Madagascar, and Uganda. Continuous clonal propagation of V. planifolia promotes
the monoculture, exposing the crop to severe damage [79] because vanilla is being
badly affected by many diseases (Table14.2.) and pests (Table14.1). Asexual propagation is a major hurdle in introduction of new genetic combination. The owers
are mostly self-pollinated, and this is a serious threat to wild populations of vanilla
[80]. So, there is dire need to search for alternative methods to induce genetic variation into vanilla gene pool due to insufcient genetic variability, the continuous
threat of destructive diseases that may wipe out vanilla crop, as well as the destruction of its natural habitats. Interspecic hybridization between available vanilla’s
primary gene pool and secondary gene pool, a close relatives of V. planifolia, can be
used to broaden the narrow gene pool. This can Improve the quality characteristics
of vanilla, i.e., higher vanillin content, improved aroma and taste, larger bean size
that may benet vanilla producers and consumers. Moreover, nonviable hybrid
seeds can be used to retrieve and regenerate immature embryos and develop it into
a complete plant.
Cell or protoplast culture can be used for creating somatic hybrids and for transfer of desirable traits from closely related sources. Clonal propagation of elite lines,
in vitro conservation, and international germplasm exchange is possible using
micropropagation techniques. DNA makers such as Amplied Fragment-Length
polymorphism (AFLPs), Random Amplied Polymorphic DNA (RAPD),
Restriction Fragment Length Polymorphism (RFLP) and biochemical markers (isozyme, protein) can be used for the identication of germplasm and somaclonal variants [6].
14.44 Vanillin Spice
It is one of the most popular and most expensive spice in the world. It is a premium
avouring agent and perfume ingredient, with high global demand. In 2015, the
market cap of synthetic vanillin was 60tons. A vanilla bean produces 2% vanillin,
with other components present that enhances its quality. The highest producer of
orchid-based vanillin in the world is Madagascar, followed by China, Indonesia and
Mexico. These states are also the biggest exporters of seed pods. USA is the biggest
importers of vanillin extract and vanilla seed pods. It is expected that the consumption of vanillin produced from vanilla orchids will grow in future. Largest use of
vanilla is in ice-creams. It is widely used in yogurt, cookies, brownies and cake.
Vanilla essence is easily available in markets [24, 81].
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