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Gum hyperplasia
Diagnostics of leukemia
1.Morphological 2.Flow cytometry 3.Immunological
4. Cytogenetic analysis 5.Molecular marrow evaluation
Morphological method
Sternal puncture |
Trepanobiopsy |
>20% blasts in bone marrow (normally < 3%).
Normal bone marrow
Acute leukemia
Acute lymphoblastic leukemia
Hodjkin`s lymphoma
Immunophenotyping
•Distinguishing between AML and ALL
•Detection of subtype of AML
•Analysis of clusters of differentiation
ALL
CD 1-10 (Т-cell markers)
CD 19, 20, 22 (В-cell markers)
AML
CD 13, 14, 33
Cytochemical study
Performed on peripheral blood smears and bone marrow smears, is based on cellular enzymatic reactions to impart staining
The acid-Schiff reaction for glycogen is «-» in |
Reaction for myeloperoxidaze from azurophilic |
granulocytic leukemia |
granules of neutrophils is «+» in granulocytic |
|
leukemia |