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33 Operative Vectors, Anatomic Distortion, andtheInherent Eects ofInsuation
not subject to collapse as this can dramatically limit the ability to continue with safe dissection.
Traditional insufators were designed to dis­tend the relatively large volume of the peritoneal cavity. Such insufation technology was actually based on a quite rudimentary mechanical model. In simple terms, the system delivers CO2 gas in a pulsed fashion via a singular disposable insufa­tion tubing. The laparoscopic insufator then senses the pressure via sampling and delivers or ceases to deliver gas in response to an arbitrary set pressure. Thus, when the pressure in the insuf­ated cavity falls to below the set level, gas is actively pumped into the cavity until the desig­nated pressure set point is reestablished. Minor
Fig. 33.4 The faceplate of the TAMIS port commonly used for taTME is shown. One of the cannulas has been replaced by an 8mm valveless trocar (AirSEAL®), which maintains a pressure barrier seal pneumatically rather than with the trapdoor design that typically smudges the lens during camera lens entry. As can be seen, the trocar is completely transparent along its long access
uctuations in pressure do not exhibit an appre­ciable effect on larger spaces such as the abdomi­nal cavity and insufation through this modality is quite reliable. However, minor uctuations in pressure in a small operative eld can result in collapse of the workspace– before the insufa­tion system can respond to the change, thereby resulting in noticeable loss of the operative eld of view (since this eld is essentially created by pneumatic distension which must remain stable). Furthermore, the restricted view is also limited as plumes of smoke often accumulate as there is poor smoke dissipation, since the smoke is not able to be distributed over the larger volume of the peritoneal cavity.
Most insufators for laparoscopy have not evolved since their inception, and have essen­tially remained unchanged in their technology over the decades. Existing systems had worked quite well for laparoscopy and given there had not been any incentive to alter this technology it remained perfectly well suited for most rudimen­tary laparoscopic procedures. However, the increasing use of complex laparoscopy and espe­cially robotics in MIS leads to renements that would serendipitously benet transanal platforms and especially TAMIS-based procedures.
Insufation system that had been developed to improve clarity with abdominal (and especially robotic) minimally invasive surgery emerged. In particular, one system (AirSEAL® iFS) was developed (originally by SurgiQuest and cur­rently, ConMed, Inc.) with the objective of utiliz-
ing a valveless trocar system that would create an invisible pressure barrier [20] so as to prevent smudging of the camera’s lens with repeated tro­car withdraws and reinsertions– a known prob­lem with trapdoor style trocars (Fig.33.4). The system also was designed to maintain stable pneumatics and to address the problem of smoke accumulation. Specically, by adapting a special­ized, no-valve trocar to triple lumen insufation tubing, (a) smoke evacuation, (b) pressure moni­toring, and (c) CO2 delivery could be separately managed. While these were considered important advantages for advanced robotic and laparo­scopic abdominal surgery [21, 22], it should be underscored that the AirSEAL® system was not designed for transanal surgery per se, nor was the system designed to rectify the problem of cyclic billowing with TAMIS. Instead, like TAMIS itself, the advantage of AirSEAL® iFS for trans­anal access in resolving the issue of pelvic breath­ing and smoke accumulation was realized completely by accident [23, 24].
Today, AirSEAL® iFS is the commonest insuf­ation system preferred by experts for use in con­junction with the TAMIS platform for both local excision and taTME to resolve the issue of cyclic billowing, which is otherwise considered to be one of the most signicant intraoperative limitations based on European Registry data [25]. However, AirSEAL® iFS may or not be available, and thus other substitutes can be considered, including a
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hand-fashioned apparatus, whereby a surgical sterile glove is used as an interposition in the CO2 tubing [26], providing a reservoir that serves as a proxy for operative space, thereby minimizing the effect of billowing, but not necessarily smoke accumulation. This makeshift solution is a useful construct and represents an important low-cost alternative to the valveless trocar system. Furthermore, in 2018, the manufacturer of the GelPOINT Path Transanal Access Platform (Applied Medical, Inc.) began including a reser­voir bag (at no additional cost) which reduces bil­lowing in the same manner [27]. There are other options that have recently become available, including PneumoClear® Insufation (Stryker, Inc. Kalamazoo, MI, USA) with TAMIS mode that is designed to achieve a more stable pneumor­ectum and pneumopelvis than standard laparo­scopic insufators.
The AirSEAL® iFS system is often incor­rectly classied as a “high-ow” insufator. In actuality, however, in AirSEAL Mode, the typical rate of ow during taTME is quite low at 8L/ min, and pressure limits are set to ~8–12mmHg. The system is designed to respond instantly to pressure changes by increasing the rate of ow. For example, if plumes of smoke or blood require ancillary suctioning to clear the eld, the process of suctioning will result in a quite sudden decrease in the pressure which can threaten the stability of the pneumatic distention essential in maintaining the operative eld of view. To com­pensate for this, the AirSEAL® iFS system is designed to increase ow to up to 40L/min tran­siently. This rapid, real-time response is one of the important factors that allows for TAMIS and taTME to be performed with a stable operative view that has minimal billowing. Cyclic billow­ing is also greatly dampened (if not completely eliminated) by the constant sampling of gas pres­sure by this system.
Even with AirSEAL® iFS and other advanced platforms, during taTME at the point of perito­neal entry, there is potential for loss of the opera­tive eld of view as pneumatic distention diminishes when the taTME insufation pressure “competes” with the abdominal insufation pres­sure. With the two-team approach, laparoscopic
access and insufation are present, and pressure settings should always be slightly less for abdom­inal insufation relative to taTME insufation. This is to maintain a positive down-to-up pres- sure gradient, otherwise the actualized work­space will collapse. This is true even if there is only one AirSEAL® iFS system in use, and a traditional laparoscopic insufator is being used to insufate the abdominal cavity. In such a set­ting, cyclic billowing can occur at the point of peritoneal entry. In general, the peritoneal entry, which is most commonly achieved along the anterior reection (as this is the shortest distance to the abdominal cavity) should be the last major step in the taTME dissection. After this step, even with correct pressure settings, a diminished oper­ative view can often be observed.

Anatomic Distortion

With abdominal minimally invasive surgery (MIS), whether laparoscopic or robotic, the insufation applied does not substantially distort the native viscera as the insufation is evenly dis­tributed over a large area, and the only noticeable distortion is the symmetrical doming of the ante­rior abdominal wall. However, during the trans­anal portion of taTME, anatomical distortion can be quite pronounced. This occurs as the operative insufation vector exerts an effect which aids in establishing the taTME dissection plane but, at the same time, creates gross anatomic distortion as the mesorectal envelope and rectum proper become mobilized (Fig. 33.5) [6]. Classically, this produces a concavity of the mesorectal enve­lope and also a forward compression of the entire rectum and mesentery that can sometimes render the anatomy unrecognizable. During the poste­rior dissection, the mesenteric distortion creates a central concavity with a ventral bend to the mes­enteric envelope (Fig.33.6a, b). As the lateral and anterior dissections are completed, the distortion compresses the entire rectum and its mesentery cephalad.
Because of the distortive effects imparted by operative vectors, the mesentery does not typically appear elliptical, and its completeness
33 Operative Vectors, Anatomic Distortion, andtheInherent Eects ofInsuation
Fig. 33.5 Gas ow for taTME is delivered via transanal access. This insufation vector matures planes naturally and is considered a fundamental component of the operation, greatly assisting with sharp dissection and with actualizing the subperitoneal workspace. However, as the planes develop, especially posteriorly, the rectum and mesentery exhibit a characteristic gross anatomic distortion, as illustrated
349
Fig. 33.6 (a) The posterior plane of dissection during taTME is shown with separation between the angel hair (cheveux d’ange) and the mesenteric envelope correctly established. Clearly shown is a concavity of the dorsal mesentery which represents gross anatomic distortion that occurs due to the insufation vector required during taTME.On occasion, such anatomic distortion can challenge the surgeon’s understanding of the operative anatomy, and this may lead to wrong-plane surgery. (b) An artist’s rendition of anatomic distortion illustrating the classic concavity of the mesorectal envelope
a
Anatomic distortion
Cheveux d’ange
b
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cannot be assessed until the specimen is extracted. This implies that the operator must instead rely on the interpretation of the fusion planes and must understand and appreciate the typical appearance of anatomic distortion during the transanal portion of the operation. This is one reason that taTME dissection presents unfamiliar anatomy to the novice surgeon and why signi­cant experience is required to gain prociency with this challenging technique.
Triangles andHalos
As a result of pneumatic dissection, release along natural anatomical planes of fusion is observed, but occasionally there are tethering points which are adherent and must be released through delib­erate sharp dissection. As the tethered point tents the fascia in the shape of a triangle, this is often recognizable as such, and thus fascial plane “tri­angles” can be an important clue as to the location of the correct plane. Such triangles occur in all aspects of surgical dissection, particularly when
tissue is placed on stretch, and when a lead point has not been released. Such phenomena are not infrequently encountered during dissection with laparoscopic and robotic colorectal surgery. Thus, triangles from tethering of unreleased points are not unique to taTME, but tend to be quite pronounced with this operation in particu­lar. When the point of tethering (usually the ven­tral tip of the triangle) is not recognized and the dissection proceeds dorsal to this point, the fas­cia is violated, and it results in disruption of the fascia plane. Because the pneumatic force is uni­formly distributed at this point of violation, the appearance of a linear fascial disruption will take on the shape of a circle and has thus been termed the “halo sign” [28]. Triangles and halos are important signs in maintaining plane recog­nition during taTME.Due to the unique fascial layering patterns, entry into false planes is quite typical during the natural course of taTME dis­section. Especially along the posterior dissec­tion, it is critical that the triangle and halo phenomena are recognized and appropriately managed (Fig.33.7).
Tethering
point
creates
‘Triangle’
Insufflation vector
Fig. 33.7 Triangles and halos are pneumatic phenomena observed during all minimally invasive surgery, but are particularly important with taTME. As originally described by Bernardi and colleagues, triangles are cre­ated when a tethering point of a fascial plane has not been released by sharp dissection. Such a point must be recog­nized and dissected free, thereby releasing the adherent fascia. This is of particular importance along the posterior dissection where the endopelvic fascia tends to be adher-
Mesorectum with mesorectal fascia
Endopelvic fascia
Mesorectum with mesorectal fascia
Halo effect or “O” Sign
ent, and when the mesentery is projected anteriorly by the insufation vector, the tethered plane “stands up” in the shape of a triangle. If this or any fascial plane is violated at a point other than its fusion point, a linear cut along the fascia takes on the appearance of a halo or circle since the pneumatic force evenly distributes tension. Triangles and halos are important clues, and taTME surgeons must remain vigilant, making plane adjustments accordingly
33 Operative Vectors, Anatomic Distortion, andtheInherent Eects ofInsuation
351

False Planes

The insufation vector of taTME affects the ante­rior dissection differently than it does to the pos­terior dissection, and this is one of the most fundamental principles to understand that is quite unique to this operative approach. For the ante­rior dissection, there is no appreciable difference in the opening of fascial planes, and operative progress is similar to the standard, up-to-down approach. This is because there is no directional layering of fascia anteriorly. In contradistinction, during the posterior and lateral dissections, there is a specic orientation to the extra-mesorectal fascia, which layers in such a way that when the dissection is carried out from below, fascial planes are pneumatically opened, as they tend to “stand up” during the taTME dissection (Fig. 33.8). Thus, during the posterior and to some degree the lateral dissection, the planes beyond the mesenteric envelope are exposed as dissection proceeds “against the grain” of fascial layering. The most pronounced effect of this is observed along (a) Waldeyer’s fascia, (b) the lat­eral fat pillars (Fig. 33.9), and (c) the inferior hypogastric roots of S2 and S3 autonomic nerve plexi (Fig.33.10).
Importantly, the tapered distal mesenteric
envelope can be dissected by airow jets causing
the mesentery itself, in some instances, to take on an areolar appearance that can be confused for a plane of dissection. However, this often leads to one of the most common errors in taTME surgery, namely, intramesorectal dissection and violation of the mesenteric envelope with consequent onco­logic compromise (when the operation is per-
Fig. 33.8 The transanal approach to taTME with the applied insufation vector from the perineum tends to “stand up” fascial planes that may lead a surgeon to enter a plane that is too deep. Here shown is the standing up of Waldeyer’s fascia. The correct plane is to proceed ven­trally along the mesorectal envelop, but a more dorsal plane deep to the endopelvic fascia is often incorrectly selected. The standing up of planes is not typical of the abdominal approach to TME and is a characteristic spe­cic to taTME
Lateral fat pad
CO
dissection lateral to correct plane
2
Fig. 33.9 Between 6 and 8cm from the anal verge at the 3 and 9 o’clock position lie avascular fat pads that are separate from the TME plane and do not follow its ellipti­cal shape (curved arrow). Pneumatic dissection due to the
Correct TME plane
insufation vectors creates a misleading, areolar plane of dissection that can incorrectly direct the surgeon to this lateral plane, which often results in sacral bleeding
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Fig. 33.10 Posterior taTME dissection is shown, whereby dashed lines in green delineate the correct plane of dissection, while a dashed red line overlies a lateral, areolar area that is an incorrect plane. Between the correct and incorrect plane lie the inferior hypogastric nerve roots from the S2 and S3 tributaries. These nerve roots, denoted
Exposed rectal wall
Fig. 33.11 It is classically stated by RJ Heald that the correct TME plane is the “innermost dissectable plane.” However, insufation vectors can dissect the mesentery itself, giving it the appearance of being correct. Here, the posterior TME plane is being dissected. Note that the
by dashed purple lines, “stand up” in a vertical orienta­tion, and they often take on the shape of a bow or shoe strings. It is imperative that taTME surgeons recognize these roots and are not drawn to more lateral areolar planes which would result in signicant autonomic nerve injury
‘Areolar’ mesentery
mesentery appears quite areolar and thus dissectable. However, in fact, it is not and instead dissection of this areolar mesentery has exposed the rectal muscle tube, which is clearly visible in this video still frame
formed for cancer). The innermost dissectable plane– as described by RJ Heald– can thus give a false appearance of having yet a more inner dis­sectable plane as the mesenteric envelope pres­ents an areolar appearance due to constant-pressure pneumatics, delivered from the taTME vantage
point. In surgical practice, this error tends to occur in the initial posterior dissection. It happens not only because of the mesentery itself becomes areolar (Fig.33.11), but because the mesenteric envelope itself may have a steep posterior slope along the sacrum requiring compulsory steep
33 Operative Vectors, Anatomic Distortion, andtheInherent Eects ofInsuation
353
angulation of the instruments during this portion of the operation to accommodate the patient’s pel­vic geometry [29].
CO2 Entrainment andEmbolization
CO2 embolization during laparoscopy can be lethal [30, 31]. While most abdominal laparo­scopic operations present at least some risk of CO2 venous entrainment and subsequent air embolization, this risk is generally nominal, and the incidence of clinically relevant air emboliza­tion during such procedures is exceedingly rare [32]. However, one of the rst small series to report outcomes with taTME by Rouanet et al. included CO2 embolism as a morbidity [33], and although unreported in the initial registry data series [34], it has now become apparent that this risk may be moderately higher than with conven­tional laparoscopy and at the time of this writing is actively being studied [35]. The most likely mechanism for this is exogenous gas entrainment into low-pressure venous vessels which may become injured during the process of taTME dis­section [6]. This may be further exacerbated by the type of insufator being used and the insufa­tor’s operational mode; however, this remains an area of ongoing investigation.
When the pressure of the venous system is less than the pneumatic pressure of insufation, insufated CO2 gas enters into the venous system where it can result in cardiovascular collapse as it creates a right ventricular airlock. In the observed events, the venous bleeding is not excessive and tends to tamponade by the force of pneumatic insufation. Because CO when the pressure exceeds venous pressure, it is strongly recommended that insufation pressure be set to less than normal venous pressure and to the lowest possible setting which allows for maintenance of the visual eld – particularly when constant ow systems such as AirSEAL® iFS are employed. Furthermore, it should be noted that venous pressure may be decreased by steep Trendelenburg positioning while ow increases by gravity and via respiration. These are factors which can exacerbate the rate of CO2 gas entrainment into lacerated vessels.
entrainment results
2
Paroxysmal and otherwise unexplained altera­tion in end-tidal CO2 (ET-CO2) should immedi­ately alert the taTME surgeon and anesthesiologist to the possibility of air embolization. This sudden change in ET-CO2 is usually the sentinel event detected, heralding the onset of cardiovascular compromise. In most instances, ET- CO2 decreases, but an increase in this parameter has also been observed during air embolization. Treatment of CO2 embolization requires rapid intervention and mandates that surgeon and anes­thetist work in concert to rectify the problem. These steps include the immediate cessation of CO2 gas delivery, ooding of the operative eld with saline, or gauze soaked in saline to prevent further gas entrainment, while controlling ongo­ing venous hemorrhage. Simultaneously, the anesthetist should perform Durant’s maneuver – that is, maintain moderate Trendelenburg (head lower than level of feet) while placing the patient in left lateral decubitus position (left-side rota­tion of the operating table); this is believed to decrease or at least limit gas from traveling through the right side of the heart into the pulmo­nary arterial tree where right ventricular outow can become obstructed due to an air lock. Furthermore, increasing positive end-expiratory pressure (PEEP) can decrease the pressure gradi­ent between the lacerated venous vessels and the central cardiovascular system, thereby limiting the potential of further gas entrainment [36, 37].
Investigation into understanding the process of gas embolization during taTME remains an area of active research. Alternative exogenous gases, unfortunately, are not currently feasible for use with taTME. For example, helium, although essentially inert with no pharmacologic effects and although noncombustible, is rela­tively insoluble in blood and more likely to result in embolization [38], leaving exogenous CO
gas
2
as the only practical option at present.
CO2 Aerosolization ofBacteria andTumor Cells
Among the pragmatic differences between taTME and other sphincter-preserving, anterior operations for extirpation of the rectum is that an
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intentional rectal wall violation (rectotomy distal to the purse string) is performed [6, 29, 39]. Theoretically, bacteria and even live exfoliated tumor cells can shed [4045], thereby seeding the pelvis during taTME.This could be related to the following factors: (a) poor mechanical bowel preparation, or, in the case of invasive cancer, observation of a friable tumor; (b) improper purse string, or purse string/rectal wall violation during taTME dissection; and (c) the aerosolization of cells by the force of CO2 insufation during the process of dissection. The theoretical implica­tions are, in the immediate postoperative time­frame, pelvic sepsis and abscess formation can ensue, and perhaps more importantly, in the long term, an increased risk of local recurrence due to tumor cell implantation may be observed. It should be noted that the latter has not been real­ized by clinically available data, which, it should be cautioned, only includes short- and midterm follow-up.
Due to the complexity of metastasis, tumor cell deposit volume, and the requirements to suc­cessfully implant a viable metastatic focus, it is probable that even live exfoliated tumor cells that seed the resection bed do not result in cancer recurrence in most instances. In contradistinc­tion, bacterial cells are easily able to thrive in the abdominopelvic cavity and probably require a lower inoculum to result in clinically relevant infection. This is particularly true when the inoc­ulum is a mixed ora of anaerobes and facultative bacteria which exhibit a synergistic effect in sep­sis [46, 47].
To minimize the risk of cell spillage, the purse string should be tightly and securely fastened and tested prior to rectotomy by insufation. Small gaps and imperfections should be oversewn. Prior to and after purse-string application, most experts recommend antiseptic-tumoricidal irriga­tion [28]. Even meticulous care may not fully prevent bacterial and (potentially) tumor cell spillage into the operative space as it is devel­oped, in part, by pneumatic dissection. In 2015 Velthuis etal. examined intra-abdominal contam­ination of bacteria during TAMIS-based taTME [48]. In this study, 23 patients underwent the operation with povidone iodine rinsing of the
rectal lumen before and after purse-string appli­cation and prior to commencing the transanal dis­section. Next, during the course of taTME dissection, cultures were obtained from the ster­ile, laparoscopic ports from the four quadrants of the pelvis, and later the patients were followed clinically. The data revealed that 39% had posi­tive cultures for enteric microbes (e.g., Escherichia Coli). Furthermore, 17% of patients had localized infections within the pelvis man­aged nonoperatively with systemic antibiotics with or without percutaneous drainage. These data suggest that despite irrigation, contamina­tion of the sterile abdominopelvic cavity occurs not infrequently during taTME and pneumatic insufation with aerosolization of microbes may be a contributing factor in some circumstances, although the exact mechanism is not known. A powerful tool in the assessment and safe imple­mentation of taTME has been the registry data, which at the time of this writing includes over 3000 cases which have been entered into the European taTME Registry [49]. These data extracted from this invaluable resource are expected to greatly enrich our understanding of this emerging operation in the coming years.
Acknowledgments The authors appreciate the invalu­able assistance of Stephanie Philippaerts and the iLapp­Surgery Foundation in the development of the medical illustrations contained in this chapter.

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