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356 Wild Edible Plants
The carbohydrates presented by easily assimilated soluble- free sugars, usually are
represented mainly by glucose and mannose, as well as its alcohol derivative mannitol,
which is an important metabolite of many mushrooms (Crizan & Sands, 1978; Miles
& Chang, 2004; Solomko, 2011). The main free sugar in C. aegerita found rich in
the carbohydrate sample (84.5 g/ 100 g DW) was trehalose while malic acid was the
most abundant organic acid (Petrović et al., 2015). The solutionable components of
carbohydrates include fructose, galactose, trehalose, and several other mono- and disaccharides usual for plant foods. However, the bulk of the total carbohydrates of all
mushrooms are polysaccharides of varying degrees of polymerization. Polysaccharides
that are difcult to hydrolyze and indigestible make up the ber fraction, which contains
the nitrogen- containing polymer of the fungal cell wall – chitin, as well as pigments
(melanins, quinones) (Solomko, 2011). Fiber contents of fruit bodies and mycelia were
in the range of 5.5– 47.0 g/ 100 g DW and H. erinaceus, L. edodes, C. aegerita presented
higher levels of dietary ber than S. rugosoannulata (Table 13.1).
Lee et al. (2009) reported that dietary ber includes polysaccharides,
oligosaccharides, lignin, associated plant substances and promotes benecial physiological effects, including relaxation, and/ or blood cholesterol attenuation, and/ or blood
glucose attenuation.
It is known that energy for all human life processes is released during the breakdown of food organic substances. An analysis of the general chemical composition,
considering the digestibility coefcients for mushroom proteins, fats, and carbohydrates,
reveals detailed insights into their nutritional value. Crisan and Sands (1978) show
that the energy value of 100 g of dried mushrooms is the maximum for L. edodes and
P. eryngii up to 392 and 421 kcal, respectively. So, mushrooms are a food with low dry
matter and lipid contents and a low energy value. This suggests that mushrooms should
be classied as low- calorie foods rich in mineral substances.
13.3 THE FUNCTIONAL FOOD
The popularity of mushrooms is due to their appealing taste, avor, and texture as food,
as well as their positive impact on health. The term “functional food” means that a
product provides sufcient nutritional effects and positively impacts one or more bodily
functions. Eating such food may lead to improved health or reduced risk of disease. It is
not tablets or capsules, rather, it is the regular dietary pattern (Vetter, 2019). The use of
mushrooms as potential functional compounds in various food applications is presented
in Figure 13.2.
Another important function of food is to supply the body with physiologically
functional, irreplaceable for humans’ substances that must necessarily be supplied
with food since they cannot be synthesized in the human body. There are not many
such scientically established substances that are not synthesized in the human body,
and they include some amino acids, unsaturated fatty acids, trace elements, and
vitamins, and they include some amino acids, unsaturated fatty acids, trace elements,
and vitamins.

Edible and Medicinal Mushrooms as an Eco-Friendly Source of Food 357
FIGURE 13.2 The scheme of influence of mushroom nutrients on quality human health
effects.
Proteins. Essential amino acids and taste components. The most decient component in
human nutrition is a complete protein. The term protein in natural food products implies
a mass of proteins, peptides, and amino acids that make up the protein component of
food – the source of amino acids necessary for humans. The primary protein sources for
humans predominantly come from animal products, mainly meat. Since more and more
individuals refuse to eat animals and adhere to a vegan or vegetarian diet, mushrooms
are a valuable protein source (González et al., 2020). Ensuring adequate protein intake
is crucial for improving muscle mass and strength. Consuming high- protein foods
reduces appetite, supports weight maintenance, and prevents overeating. The proteins
of the fruiting bodies and mycelium of cultivated mushrooms contain all 18 amino acids
included in the balanced nutrition formula, of which particular value represents the
essential ones: isoleucine (Ile), histidine (His), leucine (Leu), lysine (Lys), phenylalanine
(Phe), threonine (Thr), tryptophan (Trp), tyrosine (Tyr), and valine (Val) (Table 13.2).
Phenylalanine, tyrosine, and tryptophan, present in the studied mushrooms,
are essential aromatic amino acids, with function in the brain as precursors for
neurotransmitters, such as serotonin, and catecholamines (adrenaline, noradrenaline,
dopamine). Tryptophan is the precursor of vitamin B3 (niacin) and one of the amino
acids that stimulate the secretion of insulin and growth hormone (Bach et al., 2017).
Moreover, mushroom proteins exhibit a notable presence of branched- chain amino acids,
a characteristic commonly associated with animal- based protein sources. The content
of essential amino acids in the fruiting bodies of various cultivated edible mushrooms
can be quite high, exceeding 40% of the total amount of amino acids (Table 13.2).
For example, Liu et al. (2012) reported about 11 known amino acids in S. rugoso-
annulata, while Jing et al. (2022) found 18 out of the 20 protein- composing amino
acids, excluding asparagine (Asn) and glutamine (Gln). It is rich in all eight essential

TABLE 13.2 Comparative amino acid composition of fruiting bodies of the frequently cultivated edible and medicinal mushroom species
AMINO ACID
CYCLOCYBE
AEGERITA* HERICIUM ERINACEUS
HYPSIZYGUS
MARMOREUS LENTINULA EDODES
PLEUROTUS
ERYNGII
STROPHARIA
RUGOSOANNULATA**
Essential amino acids, g/ 100 g dry weight
Isoleucine 0.23 0.08 0.17– 0.41 0.03– 0.88 0.61 0.97– 1.20
Histidine 0.20 0.03– 0.05 0.29– 6.15 0.02– 0.52 0.41 3.04– 3.48
Leucine 0.44 0.09– 0.24 0.27– 0.67 0.05– 1.28 0.87 1.52– 2.05
Lysine 0.22 0.05– 0.10 0.35– 0.40 0.05– 1.14 0.87 0.84– 1.83
Methionine 0.03 0.11 0.09– 0.24 0.37 0.29 0.26– 0.37
Phenylalanine 0.47 0.02– 0.06 0.15– 0.45 0.04– 0.91 0.73 0.88– 1.27
Threonine 0.46 0.08 0.17– 0.56 1.11 0.84 0.97– 1.58
Tryptophan 0.04 0.01 0.42– 0.57 0.30 0.19 0.58
Valine 0.44 0.03 0.05– 0.43 1.20 0.87 1.13– 1.46
Non- essential amino acids, g/ 100 g dry weight
Alanine 1.26 0.24 1.05– 1.10 1.40 1.22 1.50– 2.20
Arginine 0.67 0.05– 0.11 0.27– 0.83 0.05– 1.38 1.95 0.29– 1.42
Aspartic acid 1.25 0.05 0.19– 0.21 1.73 1.35 1.93– 2.86
Cysteine 0.12 ND 0.11– 1.18 1.21 0.63 0.01– 0.08
Glutamic acid 2.75 0.05 1.42– 1.74 2.93 2.21 3.11– 5.05
Glycine 0.14 0.10 0.07– 0.35 1.53 1.22 0.96– 1.37
Proline 0.25 ND ND 0.98 0.61 1.05– 1.25
Serine 0.49 0.03 0.33– 0.76 1.08 0.84 1.10– 1.68
Tyrosine 0.43 0.05 0.15– 0.28 0.03– 0.81 0.63 0.76– 1.09
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358 Wild Edible Plants
Notes: Compiled by the authors’ data: Bach et al., 2017; Cohen et al., 2014; Jiang et al., 2023; Landi et al.,2017a; Lee et al., 2009; Mau et al., 2001; Wei et al.,
2023.
* Data recalculated on dry weight from 90.5% of the fruiting bodies humidity; ** data obtained from fruiting bodies dehydrated by different drying
methods (Wei et al., 2023).

Edible and Medicinal Mushrooms as an Eco-Friendly Source of Food 359
amino acids for the human body including Leu, Ile, Val, Phe, Met, Trp, Thr, and Lys.
The total content of amino acids in this mushroom ranges from 18.89% to 31.01%, with
essential amino acids (EAA) accounting for 6.54% to 11.70%, and non- essential amino
acids (NEAA) accounting for 7.19% to 19.97% (Jing et al., 2022). Hu et al. (2020)
reported similar results, highlighting that the primary amino acids identied were threonine (Thr) (maximum 19.35 g/ 100 g), glutamic acid (Glu) (maximum 10.40 g/ 100 g),
and lysine (maximum 3.52 g/ 100 g). As for NEAA, leucine, lysine, and threonine were
the most abundant, accounting for a maximum 20.0%, 18.0%, and 17.5% of total EAA,
respectively. Among NEAA, glutamic acid had the highest content to 7.15 g/ 100 g DW.
Seven essential amino acids and ten non- essential amino acids were detected in the
mushroom. Total and essential amino acid contents ranged from 20.3 to 29.65 and from
6.57 to 9.72 g/ 100 g DW, respectively. The total free amino acid content in four types
of H. marmoreus ranged from 4.69 to 12.30 g/ 100 g DW, and the highest level was in
fruit bodies of normal strain and less in white ones. The amino acid content was higher
in fruiting bodies than in mycelia (Lee et al., 2009).
Another amino acid found in mushrooms is not produced in the human body but is an
adaptive antioxidant and behaves in the human body like a vitamin – L- ergothioneine. It
has been shown that the fruiting bodies of many widely cultivated basidiomycetes contain ergothioneine, unusual sulfur- containing derivative amino acid histidine, a stable
natural antioxidant with unique properties, indicated ergothioneine concentrations in
H. erinaceus ranged from 7 to 46 mg/ 100 g fresh weight (FW) (Uffelman et al., 2023).
The variation of L- ergothioneine within some mushroom varieties is explained by
authors by differences in cultivation, handling, or degradation rates.
It is worth noting that free amino acids not only contribute to nutrition but also
play a role in determining the taste properties of mushrooms. According to the taste
characteristics, amino acids are classied into monosodium glutamate- like (MSG- like),
sweet, bitter, and tasteless ones. The most typical mushroom taste is MSG- like or umami
taste. The content of MSG- like in pileus (0.36– 1.17 g/ 100 g DW) was higher than that
of the stipe (0.32– 0.40 g/ 100 g DW) (Hu et al., 2020). Aspartic and glutamic acids,
important monosodium glutamate- like (MSG) ingredients, are the essential amino acids
that confer the umami characteristic of mushrooms (Jiang et al., 2023; Lee et al., 2009).
Contents of bitter, MSG- like, and sweet components in fruit bodies were three– four- fold
higher than those in mycelia with bitter components. It seems that fruit bodies showed
more taste characteristics than mycelia (Lee et al., 2009). In addition, MSG- like and
sweet components would be responsible for the nature taste of H. marmoreus strains.
Controversial issues related to the comprehensive assessment quality of the protein
component of mushrooms, which is based on the analysis of amino acid composition,
are devoted to the fundamental work by Crisan and Sands (1978), Miles and Chang
(2004), and Solomko (2011). The proteins of different types of edible mushrooms have
different biological values, which in some cases reach the level of animal proteins and,
in others, stand on a par with vegetable cultures. Along with some shortage of sulfurcontaining amino acids, the main limiting amino acids in fungi are leucine and isoleucine. This signicantly distinguishes oyster mushrooms’ proteins and some other
edible mushrooms from plant proteins decient in lysine and tryptophan. Therefore,
the overall biological value of plant foods can be increased by its supplementation with
mushrooms.

360 Wild Edible Plants
Fats. Lipids. Fatty acids. Mushrooms contain relatively low fat content (0.3– 8.0 g/
100 g DW) (Table 13.1), representing various lipid compound classes, including free
fatty acids, monoglycerides, phospholipids, and sterols. The amount of unsaturated fatty
acids, including monounsaturated fatty acids (MUFA), and essential polyunsaturated
acids (PUFA), consisted of 50% of total fatty acids in oyster mushrooms (Solomko
et al., 2011). According to the literature, consuming unsaturated fatty acids, especially
long- chain PUFA, is necessary to reduce blood cholesterol levels and regulate cellular
physiology. The most important fatty acids found in edible mushrooms include linoleic, linolenic, and oleic acid. Linoleic acid has an effective role in lowering the level
of lipids in the blood as well as helping in the reduction of arthritis (Assemie & Abaya,
2022). Sande et al. (2019) reported that about 70.0% of the fatty acids in the total lipid
content of edible mushrooms worldwide are represented by linoleic, linolenic, and oleic
acids. Among them, linoleic acid is found in greater abundance, oleic acid is the second
most abundant, and linolenic acid is the least reported in this food type. Regardless of
the species consumed, this prole corroborates mushrooms as a vital source of essential fatty acids in the human diet. However, their concentration varies considerably
according to the location in which they grow. Therefore, compared to other foods of
vegetal and animal origin, mushrooms have the advantage of possessing high levels of
PUFA. The percentage of these fatty acids (in 100 g of total fatty acids) in mushrooms
varies greatly: linoleic acid ranges from 0.0– 81.1%, oleic acid between 1.0 and 60.3%,
and linolenic acid from 0.0– 28.8%.
A total of 24 fatty acids were identied in the mycelial mass of H. erinaceus, ten
of them were saturated fatty acids (SFA) and 14 were MUFA and PUFA (Mykchaylova
et al., 2023, 2024). The main fatty acids in the composition of all studied samples also
were linoleic acid, oleic acid, and palmitic acid, with a content of 19– 49%. According to
health recommendations, the PUFA/ MUFA ratio, which indicates the nutritional quality
of dietary lipids, should be >0.4 (Stabnikova & Paredes- Lopez, 2024). Three names
of ω- 9 MUFA (С18:1 ω- 9, С20:1 ω- 9, С22:1 ω- 9), three names of ω- 3 MUFA (С20:5
ω- 3, С20:3 ω- 3, С22:3 ω- 3), four names of ω- 6 MUFA (С18:2 ω- 6, С20:3 ω- 6, С20:2
ω- 6, С20:4 ω- 6), which belong to the group of essential fatty acids, were identied in
H. erinaceus mycelium (Mykchaylova et al., 2023).
As for the fatty acid composition in C. aegerita (Table 13.3), the most dominant
fatty acid is linoleic acid (ω- 6) (78.40%), followed by palmitic (13.07%), oleic and stearic acids (3.03% and 2.13%, respectively (Petrović et al., 2015).
Although the fat content of C. aegerita is low, the concentration of unsaturated
fatty acids is high, making it an important source of essential fatty acids in a healthy
human diet (Kalač, 2009; Li et al., 2024). The prevalence of PUFA over MUFA (in case
of C. aegerita 78.60% over 3.47%), and the determination of a high amount of linoleic
acid are signicant factors in dening the mushroom as healthy food (Miles & Chang,
2004). Unsaturated fatty acids are essential for human health, having a strong benecial effect in preventing and managing cardiovascular diseases, triglyceride levels, and
blood pressure. In contrast, saturated fatty acids, which are present in higher amounts in
food of animal origin, are associated with increased levels of triglycerides in the blood
and commonly are associated with hypertension.
The fatty acid composition in S. rugosoannulata is shown in Table 13.3. Oleic acid
(63.12%), linoleic acid (45.42%), and palmitic acid (9.46%) were the main fatty acid

TABLE 13.3 Total fatty acids’ composition in fruiting bodies and mycelial biomass of the frequently cultivated edible and medicinal mushroom
species
COMPONENTS
CYCLOCYBE
AEGERITA HERICIUM ERINACEUS LENTINULA EDODES
PLEUROTUS
ERYNGII
STROPHARIA
RUGOSOANNULATA
Saturated Fatty Acids (SFA), content (%)
Myristic acid (С
Pentadecanoic acid (C
) 0.23 0.30 (0.25)* 0.07– 0.35 (0.83)* ND ND
14:0
) 0.43 1.30 (0.63)* 2.00 (2.24)* ND ND
15:0
Palmitic acid (C 16:0) 13.07 24.00 (14.0– 19.10)* 13.70– 15.81 (17.48)* 12.80 (14.03)* 0.25– 9.46
Margaric acid (С
Stearic acid (С
Arachidic acid (С
Heneicosanoic acid (С
Lignoseric acid (С
) 0.25 0.48* 1.34* 0.13* ND
17:0
) 2.13 8.10 (0.88)* 1.88– 3.01 (1.37– 4.10)* 1.7 (9.18)* 3.95– 8.97
18:0
) 0.49 0.02* ND 4.43 0.15 – 0.47
20:0
) 0.05 0.40* ND ND ND
21:0
) 0.45 (0.71– 1.90)* 0.88 ND ND
24:0
Monounsaturated Fatty Acids (MUFA), content (%)
Myristoleic acid (С
Pentadecenoic acid (С
Palmitoleic acid (С
Heptadecenoic acid (С
Oleic acid (C18:1
Gondoic acid (C
20:1 ω– 9
) 0.01 0.35* ND ND 0.25– 0.48
14:1 ω– 5
) <1.00 1.33* 1.77* ND ND
15:1
) 0.29 0.50 (0.53)* 0.60 (2.11)* 0.72 0.94– 1.27
16:1 ω– 7
) <1.00 0.55* 0.80* 1.36 ND
17:1
) 3.03 33.70 (27.75– 37.20)* 3.46– 5.65 (11.38*) 12.30 (18.79)* 39.32– 63.12
ω– 9
) 0.05 ND ND ND 0.53– 3.21
Polyunsaturated Fatty Acids (PUFA), content (%)
Linoleic acid (C18:2 ω– 6) 78.40 26.90 (32.80– 46.96)* 67.79– 75.80 (46.03)* 68.80 (39.03)* 8.69– 45.42
Edible and Medicinal Mushrooms as an Eco-Friendly Source of Food 361
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362 Wild Edible Plants
constituents (Liu et al., 2012). MUFA and PUFA were the main groups of fatty acids in
S. rugosoannulata (Jiang et al., 2023). Oyster and king oyster (P. eryngii) mushrooms
gave the highest MUFA contents with similar amounts of PUFA, MUFA, and SFA in
both samples (Reis et al., 2012). This is consistent with the previous observation, that in
mushrooms, unsaturated fatty acids predominate over saturated (Kalač, 2009).
Minerals. Edible mushrooms are a good source of essential minerals for humans. In
the fruiting bodies of various types of wild and cultivated mushrooms, the total content
of mineral substances (ash) can reach more than 13% (Table 13.4).
The total content of macroelements important for human nutrition, which include
K, P, Na, Ca, and Mg, reaches 60– 70% of the mass of ash of wild and cultivated species
of edible mushrooms (Kalač, 2009). Mushrooms are particularly rich in potassium and
phosphorus – macroelements, the content of which can be up to 50 and 16% of the
ash mass, respectively (Vetter, 2019). Potassium (K) is the primary and rst mineral
component in all mushrooms, including wild- growing species. The level of K in caps
is signicantly higher than in stipes. Proper K is an essential element of our up- to- date,
healthy nutrition, so the K content of food is also a factor of medical signicance. The
biological importance of phosphorus- containing molecules (P) is beyond doubt. The
P- content of cultivated mushrooms (Table 13.4) is remarkable, but the variability of
these data is signicantly high. The Mg- level is high and varies between 8– 16 mg/ 100
g DW. Bell et al. (2022) wrote that sodium and potassium are crucial in preserving
the osmotic equilibrium in animal systems between intestinal uid and cells. Edible
mushrooms are effective at lowering blood pressure, reducing the risk of osteoporosis,
and maintaining bone health. Additionally, the high potassium and low Na salt content
in mushrooms made them a useful meal to ght high blood pressure and cardiovascular disorders (Desisa et al., 2024). Mushrooms contain many microelements, some
of which are decient in the human diet: iron, cobalt, molybdenum, and selenium,
which are part of the coenzymes present in many biochemical metabolic processes of
life support. The content of Fe, Cu, Zn, and Mn in industrially cultivated mushrooms
can vary widely, which is undoubtedly due to the different content of these elements
in the substrate. It is known that iron plays a crucial role in the production of hemoglobin and its deciency leads to anemia. Most iron can be found in meat products,
but many people do not eat meat now and may lack this microelement. Mushrooms
contain a healthy level of iron. Thus, in fruiting bodies of S. rugosoannulata can be
from 1.65 to 2.24 mg iron per 100 g DW (Wei et al., 2023). The content of selenium,
an important factor in maintaining health, can range from 0.012 to 20 mg/ kg DW
of fruiting bodies (Niedzielski et al., 2014). Selenium- enriched cultivated fungi can
occupy a special niche among functional foods and special food additives as important
sources of “organic” selenium (Morris et al., 2016). However, the selenium content in
such products should be standardized and regulated because in large doses it is toxic.
Huang et al. (2023) reported that S. rugosoannulata fruiting bodies could be excellent
carriers of organic Zn and Se to humans. The mycelium of S. rugosoannulata cultured
in a Zn- Se- rich liquid medium can produce organic Zn of up to 21.0 mg/ 100 g and
organic Se of up to 82 mg/ 100 g DW. The fruiting body of S. rugosoannulata grown in
the wild can contain Se up to 0.26 mg/ 100 g DW (Jiang et al., 2023). The differences
in mineral content observed among fungi may be largely due to the different mineral
composition of the substrates used for their cultivation (Bach et al., 2017; Desisa et al.,
2024; Lee et al., 2009).

TABLE 13.4 Content of minerals in fruiting bodies of the frequently cultivated edible and medicinal mushroom species
COMPONENTS
CYCLOCYBE
AEGERITA
HERICIUM
ERINACEUS
HYPSIZYGUS
MARMOREUS
LENTINULA
EDODES
PLEUROTUS
ERYNGII
STROPHARIA
RUGOSOANNULATA
The most important macroelements, g/ 100 g dry weight
Potassium, K 1.4– 3.9 4.4 1.2– 3.8 0.9– 2.7 1.6– 2.9 1.63– 4.2
Phosphorus, P 1.2 0.9 0.86 0.65– 0.87 0.90 0.80– 0.97
Sodium, Na 0.025 0 0.01– 0.03 0.013– 1.08 0.010 0.8– 1.2
Magnesium, Mg 0.16 1.17 0.2– 1.05 0.13– 0.25 0.018– 0.13 0.082– 0.126
Calcium, Ca 0.016– 0.025 0.025 0.004– 0.006 0.011– 1.260 0.017– 0.025 0.1– 1.4
The most important microelements, mg/ 100 g dry weight
Iron, Fe 0.49– 27.3 0.69 0.74– 7.79 1.2– 30.0 3.0– 37.1 2.0– 22.0
Zinc, Zn 8.6 7.4 5.0– 13.4 3.5– 9.2 6.0 4.8– 5.3
Copper, Cu 2.76 1.77 0.73– 1.84 0.52 0.5 0.3– 2.1
Manganese, Mn 0.98 1.8 1.6 1.7– 2.1 0.9– 2.1 0.6– 2.7
Selenium, Se 0.045 0.002 0.004 0.002– 0.093 0.001 0.160– 0.252
Edible and Medicinal Mushrooms as an Eco-Friendly Source of Food 363
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Notes: Data based on dry weight and obtained from wild and cultivated fruiting bodies dehydrated by different drying methods (Desisa et al., 2024; Kała et al.,
2022; Liu, 2012; Solomko, 2011; Wei et al., 2023).

364 Wild Edible Plants
Due to the possible accumulation of salts of heavy metals and radioactive elements
in the fruiting bodies of mushrooms growing in nature, much attention should be paid
to monitoring the content of lead, mercury, cadmium, and radionuclides, taking into
account their toxic effect on the human body.
Vitamins, like microelements, are the most essential irreplaceable physiologically
functional micronutrients a person must receive from food or from external sources.
Their long- term absence from the diet leads not only to metabolic disorders but also to
various diseases. Edible mushroom fruit body contains vitamins, especially B1, B2, C,
E, and D2. B- group vitamins hold utmost signicance as bioactive substances, given
their involvement in protein biosynthesis and their functional roles in the central cardiovascular, gastrointestinal, and nervous systems. Nevertheless, these vitamins cannot
be synthesized within the human body and must be acquired through supplementary
sources. The B vitamins such as thiamine, riboavin, pyridoxine, pantothenic acid,
nicotinic acid, nicotinamide, folic acid, and cobalamin have been the most frequently
observed (Assemie & Abaya, 2022). Fruiting bodies of L. edodes are rich in niacin
and ascorbic acid (vitamin C), the content of which is 12.0– 54.9 and 25.0– 60.0 mg/
100 g DW, respectively. In the fruiting bodies of this fungus, were found also, mg/ 100
g DW: thiamine B1, 0.4– 7.8; riboavin B2, 0.2– 4.9; folic acid, 0.30, and B12 0.8– 5.6
µg/ 100 g DW. Lack of folic acid in the human body leads to disorders of hematopoietic functions. Data obtained by Mattila et al. (2001) reported the highest content this
vitamin was in Pleurotus spp. fruiting bodies 0.64– 1.4 mg/ 100 g DW. So, mushrooms
could enrich with vitamin dietary food and play an important role in preventing chronic
diseases (Badalyan et al., 2019; Mehrotra et al., 2014). Mushrooms also contain ergos terol, a precursor of vitamin D2, which is produced under exposure to ultraviolet (UV)
light and is important for maintaining healthy bone structure, helps regulate the body’s
use and absorption of calcium and phosphorus, and is also known for its effective role
in antioxidant properties (Assemie & Abaya, 2022). Vitamin D deciency can lead to
osteoporosis and other bone diseases, on the other hand, it helps ght depression and
increases energy. The fruiting bodies of L. edodes contain signicantly more vitamin
D (22‒110 µg/ 100 g DW) than wild mushrooms (2.91‒29.82 µg/ 100 g DW) (Mattila
et al., 2001). It has been shown that vitamin D2- enriched mushroom A. bisporus may
provide a dietary source of vitamin D2 and other bioactive molecules to prevent cognitive abnormalities associated with dementia (Bennett et al., 2013). However, such
a study needs further clinical trials. It was reported that exposure of the mushrooms
during cultivation or later to UV light could increased the vitamin D content (Drori
et al., 2016). UV- irradiation is commonly used to produce high levels of vitamin D2 in
cultivated mushrooms. Phillips et al. (2011) reported that vitamin D2 was low, g/ 100 g
FW: A. bisporus (white button, crimini, portabella) and Flammulina velutipes, 0.1‒0.3;
moderate in L. edodes and Pleurotus spp., 0.4‒0.7; high in Grifola frondosa and other
wild growing mushrooms, 5.2‒28.1, and UV- treated portabella 3.4‒20.9. Ergosterol,
mg/ 100 g FW: was highest in G. frondosa, 79.2; L. edodes, 84.9; lowest in Morchella
spp., 26.3, and F. velutipes 35.5; the range was <10 mg/ 100 g FW among white button
composites but 12‒50 mg/ 100 g FW among samples of other types.
Ergothioneine is a thiol derivative of histidine, only obtained through dietary
intake, and able to accumulate even at high concentrations in some cells and tissues,

Edible and Medicinal Mushrooms as an Eco-Friendly Source of Food 365
owing to an organic cation transporter. Roda et al. (2021) reported ergothioneine
amount in the mycelium of H. erinaceus was 58 mg/ 100 g and 34 mg/ 100 g in its
fruiting bodies. The vitamins of S. rugosoannulata basidiocarps included ascorbic acid
and ergosterol with content ranging from 83 mg/ 100 g and 789 mg/ 100 g, respectively
(Liu et al., 2012). Jing et al. (2022) revealed that it was rich in six group- B vitamins
including B2, B6, B12, folic acid, niacin, especially the content of niacin was as
high as 39.21 mg/ 100 g DW. Folate is crucial for maintaining optimal brain function,
particularly during pregnancy. Nicotinic acid is one of the forms of vitamin B3 and
is involved in many redox reactions, the formation of enzymes, and the metabolism
of lipids and carbohydrates in living cells. The contents of both folic and nicotinic
acids in S. rugosoannulata mushrooms were the highest. This mushroom seems to be
relatively rich in ergosterol, and the relatively high ergosterol content could be of signicance for vegetarians and vegans who have limited intake of ergocalciferol from
animal origin.
Participating in the metabolism of cell membranes and inhibiting the peroxidation
of polyunsaturated fatty acids, vitamin E (α- tocopherol) has the most signicant biological activity among several known tocopherol isomers. The health benets of tocopherol as a bioactive compound are well documented. α- tocopherol, the principal form
of vitamin E, is a lipid- soluble antioxidant, and its functions as a chain- breaking antioxidant for lipid peroxidation in cell membranes (Barros et al., 2008). Ascorbic acid
and tocopherol are known to be important in safeguarding against free- radical- mediated
tissue injuries. The total content of tocopherols in the fruiting bodies of L. edodes was
about 12.5 mg/ 100 g DW, in Pleurotus spp. 24– 45 mg/ 100 g DW (Yang et al., 2013).
The simultaneous presence in mushrooms of vitamins C, D, and E, along with the
presence of many phenolic compounds, explains their benecial antioxidant properties, an effect noted in biological experiments on animals (Ferreira et al., 2009; Hobbs,
2023). Petrović et al. (2015) reported that four isoforms of tocopherols were identied
in C. aegerita fruiting bodies: γ- tocopherol was the dominant isoform with 86.08 g/
100 g DW, followed by β- tocopherol, δ- tocopherol, and α- tocopherol (8.80; 3.40, and
2.10 g/ 100 g DW, respectively). Thus, the data from a detailed analysis of the composition of nutrients of certain types of cultivated edible mushrooms provide sufcient
reasons to classify them as special low- calorie foods rich in physiologically functional
essential substances.
Fiber and other substances included in the composition of mushrooms. Edible
mushrooms, also known as macrofungi, are a valuable source of dietary bers. The
cell walls of mushrooms comprise a combination of brillar and matrix components,
including chitin and polysaccharides such as (1→3)- β- D- glucans and mannans. These
components are indigestible carbohydrates resistant to human enzymes, making them
a rich dietary ber source. Carbohydrates constitute a signicant portion of mushroom composition, ranging from 35 to 70% DW across different species. Most of these
carbohydrates are non- digestible, encompassing oligosaccharides like trehalose and cell
wall polysaccharides such as chitin, β- glucans, and mannans (Assemie & Abaya, 2022).
While the chitin content in various mushrooms typically represents a small percentage
of the total dry matter, β- glucans can be shown in high concentrations (Cheung, 2013;
Ramesh et al., 2019). There is a lot of mushroom ber in the fruiting bodies of many
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