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Файл:Ординатура / Хирургия / Библиотека им академика М.И. Перельмана / Книга_5857_Библиотеки_им_академика_М_И_Перельмана.pdf
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- •Foreword
- •Contributors
- •Preface
- •Contents
- •1. General Pharmacology
- •2. Pharmacology of Peripheral Nervous System
- •3. Pharmacology of Cardiovascular System
- •4. Drugs Acting on Urinary System
- •5. Drugs Acting on Respiratory System
- •6. Pharmacology of Central Nervous System
- •7. Chemotherapy
- •8. Autacoids and their Antagonists
- •9. Pharmacology of Drug Acting on theGastrointestinal Tract
- •10. Immunopharmacology
- •11. Vitamin and Minerals
- •12. Hormones
- •1. Introduction to Pharmacognosy
- •2. Sources and Classification of Crude Drugs
- •3. Factors Influencing Quality of Crude Drugs
- •4. Techniques in Microscopy
- •5. Introduction of Phytoconstituents
- •6. Glycosides
- •7. Alkaloids
- •8. Terpenoids, Volatile Oils and Resins
- •9. Principles of Plant Classification
- •10. Pharmaceutical Aids
- •11. Plant Products
- •12. Toxic Drugs
- •13. Poisonous Plants
- •14. Enzymes
- •15. Quantitative Microscopy
- •16. Biogenetic Pathways
- •17. Herbarium
- •18. Herbal Formulation
- •19. Plant Tissue Culture
- •20. Herbal Cosmetics
- •21. Herbal Formulation
- •1. Cellular Components
- •2. Carbohydrates
- •3. Proteins
- •4. Lipids
- •5. Vitamins
- •6. Biological Oxidation and Reduction
- •7. Enzymes
- •8. Nucleic Acids
- •9. Hereditary Diseases
- •1. Plant Cell
- •3. Fermentation
- •4. Recombinant DNA Technology
- •5. Proteomics
- •1. Introduction to Microbiology
- •2. Microscopy
- •3. Staining Methods
- •4. Biology of Microorganisms
- •5. Fungi and Viruses

287. Use(s)ofplantagospeciesis/are:
A. Reduce blood glucose
B. Increase blood glucose
C. Increase blood plasma protein
D. All of these
288. Antioxidant activity of plantago is due to:
A. Polysaccharide B. Alkaloid
C. Pectin D. Flavinoid
289. Phenoliccompoundsofplantagoworkas:
A. Anti-UV radiation
B. Anti-infrared radiation
C. Anti-electromagnetic wave
D. All of the above
290. Medicinal use(s) of plantago seed is/are:
A. Laxatives B. Elixirs
C. Liniments D. All of these
291. Botanical name of turpentine is:
A. Acasia hybryda
B. Fraxinus nigra
C. Pinus palustris
D. Batula papyrifera
292. Select the shape of leaves of pine tree?
A. Elliptic-shaped B. Needle-shaped
C. Linear-shaped D. Deltoid-shaped
293. Which is the exposed part of closed cone of pine?
A. Apophysis B. Epiphysis
C. Epicarp D. Endocarp
294. Time required for maturation of seed cones in pine
trees is:
A. 1 year B. 4 years
C. 2 months D. 2 years
295. Important constituent(s) of turpentine is/are:
A. Pinene B. Indole acetic acid
C. Piperine D. Both A and B
296. In which species needle-shaped leaves are absent
in fascicles of pine tree?
A. Pinus monophylla
B. Pinus palustris
C. Pinus lumholtzii
D. Pinus leiophylla
297. Turpentine is a chemical constituent in
___________________________.
A. Camphene B. Phenol
C. Alcohol D. Ether
298. Use(s)ofturpentineis/are:
A. Solvent B. Oxidant
C. Decomposer D. All of these
299. Turpentine is used in the production of:
A. Disinfectant B. Antibiotic
C. Antiseptic D. All of these
300. Choose the correct option solublility of turpentine:
A. Water B. Ether
C. Phenol D. All of these
301. Botanical name of black pepper is:
A. Mangifera indica B. Piper nigrum
C. Centellaa siatica D. Pisum sativum
302. Choose the fruit type of black pepper:
A. Dried and unripe fruit
B. Ripe and dried fruit
C. It is a drupe fruit
D. Both A and C
303. Select the function of body improved by
consumption of black pepper:
A. Respiration B. Reproduction
C. Digestion D. Urinary system
304. Choose the correct option on size of the fruit of
black pepper.
A. 10 mm B. 5 mm
C. 5 inches D. 5 cm
305. Select the types of roots present in pepper plant.
A. Aerial roots
B. Haustorial roots
C. Epiphytic roots
D. None of the above
306. On maturity, mostly how many seeds the fruit of
black pepper contains?
A. Two seeds
B. Single seed
C. More than 5 seeds
D. Sometimes 4 seeds and sometimes 6 seeds
307. Main constituent(s) of black pepper is:
A. Piperine
B. Omega 3 fatty acids
C. Triglycerides
D. L-citronellol
308. Molecular formula of piperine is:
A. C
10H15NO3
C. C15H15NO3 D. C2H5NO
B. C17H19NO
3
3
309. Choose medicinal use(s) of black pepper.
A. Rubefacient B. Antioxidant
C. Antipyretic D. All of these
310. Select the concentration of active constituent
piperine in black pepper.
A. 15–18% B. 4–5%
C. 10–11% D. 6–9%
Section 2 Pharmacognosy
259

ANSWER KEY
1. D 2. C 3. B 4. B 5. C 6. D 7. B 8. C 9. D 10. A 11. A 12. C 13. B 14. D
15. B 16. C 17. C 18. C 19. B 20. B 21. D 22. D 23. B 24. B 25. D 26. D 27. D 28. B
29. D 30. D 31. A 32. D 33. B 34. A 35. C 36. D 37. D 38. A 39. C 40. A 41. A 42. B
43. C 44. C 45. C 46. D 47. C 48. B 49. D 50. D 51. A 52. B 53. B 54. B 55. D 56. D
57. D 58. B 59. B 60. A 61. A 62. B 63. C 64. B 65. A 66. D 67. B 68. D 69. A 70. A
71. D 72. D 73. C 74. A 75. D 76. A 77. D 78. B 79. B 80. B 81. A 82. D 83. B 84. B
85. A 86. D 87. D 88. A 89. B 90. D 91. D 92. A 93. B 94. B 95. B 96. A 97. B 98. D
99. A 100. D 101. D 102. C 103. A 104. D 105. C 106. D 107. D 108. D 109. C 110. D 111. A 112. A
113. B 114. B 115. D 116. D 117. D 118. D 119. D 120. B 121. D 122. D 123. B 124. B 125. D 126. D
127. D 128. D 129. B 130. A 131. D 132. B 133. B 134. A 135. D 136. C 137. D 138. D 139. B 140. D
141. D 142. D 143. C 144. B 145. D 146. D 147. D 148. C 149. D 150. D 151. B 152. A 153. C 154. B
155. A 156. D 157. D 158. A 159. D 160. D 161. C 162. B 163. D 164. A 165. C 166. B 167. A 168. D
169. B 170. D 171. D 172. B 173. D 174. B 175. D 176. D 177. B 178. D 179. C 180. B 181. D 182. A
183. B 184. A 185. D 186. D 187. D 188. C 189. A 190. C 191. D 192. C 193. C 194. C 195. D 196. D
197. D 198. D 199. D 200. B 201. A 202. C 203. B 204. C 205. D 206. C 207. D 208. C 209. C 210. D
211. D 212. D 213. B 214. D 215. D 216. D 217. D 218. C 219. A 220. C 221. A 222. A 223. C 224. B
225. C 226. A 227. A 228. D 229. D 230. B 231. D 232. B 233. A 234. D 235. A 236. D 237. D 238. D
239. B 240. D 241. A 242. C 243. B 244. C 245. C 246. A 247. D 248. D 249. C 250. A 251. D 252. C
253. D 254. D 255. D 256. D 257. C 258. D 259. D 260. B 261. D 262. D 263. D 264. D 265. C 266. D
267. C 268. C 269. D 270. D 271. A 272. A 273. C 274. D 275. A 276. D 277. D 278. C 279. D 280. C
281. A 282. B 283. A 284. A 285. D 286. D 287. A 288. D 289. A 290. A 291. C 292. B 293. A 294. D
295. A 296. A 297. A 298. A 299. C 300. B 301. B 302. D 303. C 304. B 305. A 306. B 307. A 308. B
309. D 310. D
Section 2 Pharmacognosy
260

12. Toxic Drugs
Common Toxins with their Examples and Effects
Common toxins Examples Effects
Alkaloids Solanine, tomatine 20 mg/100 g of potato and 36 mg/100 g of
tomatoes is unfit to eat
Enzyme inhibitors Protease inhibitors Inhibit trypsin, chymotrypsin, and other protein
digesting enzymes
Saponins Disrupt red blood cells, cause diarrhoea, vomiting
and It may reduce blood serum cholesterol
Cyanogenic glycosides, dhurrin and amygdalin,
almonds (bitter almonds), sorghum, choke and
pin cherries, and pits of apple, apricot, cherry,
plum
Plant phenolics Safrole and coumarin Quite toxic and have been
Tannins Bananas, grapes, raisins, sorghum,
spinach, red wine, beer
Fats, sugars,
proteins,
and vitamin
antagonists
Rancid or oxidized fat erucic acid (mustard,
rapeseed)
Sugars, proteins and vitamin
antagonists
12 bitter almonds can kill a child.
banned
Cause of enzymatic browing, bind protein,
precipitate protein of epithelium, cause liver
damage, inhibit virtually every digestive enzyme
Liver degeneration and kidney nephrosis
headache, dizziness, and cramps facial flushing,
rapid heart rate, increased blood pressure, and
headaches
Tumor promoters
13. Poisonous Plants
Plant Producing Systemic Poisoning
Family Examples
Berberidaceae Podophyllum
Euphorbiaceae Euphorbia, Ricinus and Jatropha
Leguminosae Cassia, Abrus and Gymnocladus
Ginkgoaceae Ginko
Apocynaceae Thevetia, Acokanthera
Liliaceae Allium, Aloe, Scilla and Urginea
Solanaceae Atropa, Capsicum, Datura, Hyoscyamus and Nicotiana
Taxaceae Taxus
Loganiaceae Strychnos and Gelsemium
Meliaceae Melia and Swietenia
Umbelliferae Conium, Oenanthe
Polygonaceae Rheum
Scrophulariaceae Digitalis
Rosaceae Prunus, Malus and Eriobotrya
Section 2 Pharmacognosy
261

List of Poisonous Plants with their Description
Poisoning of plants
with
Anticholinergic
poisons
Calcium oxalate
crystals
Cardioactive
steroids/cardiac
glycosides
Convulsant
poisons (seizure)
Cyanogenic
compounds
Gastrointestinal
toxins
Section 2 Pharmacognosy
Examples Toxins’ mechanism Clinical manifestations Specific therapeutics
Atropa
Datura
Hyoscyamus
Solanum
Competitive antagonism
of acetylcholine at the
muscarinic subtype of the
acetylcholine receptor
Dry, warm, and ushed
skin
Garbled speech
Sinus tachycardia
Urinary retention
Delirium with
It includes sedation
with a benzodiazepine
or reversal of their
clinical syndrome with
physostigmine
hallucinations
Brassaia
Caladium
Caryota
Philodendron
Calcium oxalate crystals
penetrate the mucous
membranes and induce
the release of histamine
and other inflammatory
mediators
After biting or chewing
Local oropharyngeal
pain, local swelling
garbled speech
After swallowing
Inammation of the
Demulcents, viscous
lidocaine, analgesics or
with copious irrigation
posterior oropharynx
Oropharyngeal edema
Airway compromise
Adonis
Calotropis
Digitalis
Thevetia
Indirectly increase
intracellular Ca
concentrations in
myocardial cells.
Enhance cardiac
+2
induce vomiting which
serves both as an early
sign of toxicity to limit
poisoning
Abdominal pain and
Antigen-binding regions
(Fab) of animal-derived
antidigoxin antibodies
inotropy (contractility)
and slowdown the heart
rate
Caulophyllum
Lobelia
Myoporum
Nicotiana
Seizures including
antagonism of gammaaminobutyric acid
(GABA) imbalance
of acetylcholine
homeostasis,
excitatory amino acid
mimicry, sodium
Tonic-clonic
convulsions,
Loss of consciousness
as a result of central
nervous system
dysfunction, urinary or
fecal incontinence
Tongue biting
Anticonvulsant
benzodiazepine or
lorazepam, should be
administered parenterally
for persistent seizures
channel alteration, or
hypoglycemia
Eriobotrya
Hydrangea
Malus
Prunus
Cyanide inhibits the final
step of the mitochondrial
electron transport chain,
resulting rapidly in cellular
energy failure
Abdominal pain, vomiting,
lethargy, and sweating,
altered mental status,
seizures, cardiovascular
collapse, and multisystem
Aggressive supportive care,
intravenous fluid therapy,
intravenous sodium
bicarbonate and antidotal
therapy
organ failure
Irritant toxins indirectly
stimulate contraction
of the gastrointestinal
smooth muscle.
Hepatotoxins may directly
injure the liver cells,
commonly through the
production of oxidant
Nausea, vomiting,
abdominal cramping, and
diarrhoea
Vomiting may be mitigated
by antiemetic agents
such as metoclopramide;
occasionally,
resistant emesis may
require a serotonin
antagonist such as
ondansetron
metabolites
(Contd.)
262

Poisoning of plants
with
Mitotic inhibitors Catharanthus
Nicotine-like
alkaloids
Pyrrolizidine
alkaloids
Examples Toxins’ mechanism Clinical manifestations Specific therapeutics
Colchicum
Gloriosa
Podophyllum
Caulophyllum
Conium
Gymnocladus
Lobelia
Nicotiana
Echium
Heliotropium
Senecio
These agents interfere
with the polymerization of
microtubules, which must
polymerize for mitosis
to occur, leading to
metaphase arrest
These agents are directacting agonists at the
nicotinic subtype of the
acetylcholine receptor in
the ganglia of both the
parasympathetic and
sympathetic limbs of
the autonomic nervous
system
Pyrrolizidine alkaloids are
metabolized to pyrroles,
which are alkylating
agents that injure the
endothelium of the hepatic
sinusoids or pulmonary
vasculature
Gastrointestinal
abnormalities, including
vomiting and diarrhoea.
Nervous system toxicity,
including ataxia,
headache, seizures, and
encephalopathy
Sympathetic stimulation,
including hypertension,
tachycardia, and
diaphoresis, and
parasympathetic
stimulation, including
salivation and vomiting
Acute hepatotoxicity
caused by massive
pyrrolizidine alkaloid
exposure
produces gastrointestinal
symptoms, right upper
quadrant abdominal pain,
hepatosplenomegaly,
and jaundice as well as
biochemical abnormalities
consistent with hepatic
necrosis
Initial management
includes aggressive
supportive and
symptomatic care
Antihypertensive drugs,
including nitroprusside or
diltiazem, seizures should
respond to intravenous
benzodiazepine,
such as lorazepam or
diazepam
Standard supportive care,
liver transplantation
14. Enzymes
Types of Enzymes with Example
Type of enzymes Example
Animal enzymes Lipases, rennets, tripsin
Higher-plant enzymes Amylases, papain, proteases, and soya bean lipoxygenase
Microorganisms Acetobacter lacti, Clostridium aceticum
Food and beverage enzymes Papain, protease
Classicationoftheenzymes based on their site of action:
a. Endoenzymes (intracellular enzymes): The enzymes
thataresolelysecreted verymuchwithinthecellare
termed endoenzymes. Theyessentially are involved
inthe apt synthesis ofdifferent cellular components,
foodreserves,andalsoserveasbioenergeticmaterials.
Importantly, as thesevarious processes do occur in
theintracellular zones,the enzymes involved are
also strategically located in the intracellular region,
e.g. isomerases, phosphorylases, synthetases, etc.
b. Exoenzymes (extracellular enzymes): The enzymes that
areexclusivelysecretedoutsidethecellareinvariably
known as exoenzymes or extracellular enzymes. They
usuallyexertadigestivefeatureintheiroverallactivity
andfunction.Interestingly,theyhelpinthehydrolysis
ofrelatively complex molecules into much simpler
compoundsfortheformationofangiotensinIIsolely
required to maintain and raise the blood pressure.
Examples: Amylases—hydrolyse starch components;
Lypases—hydrolyse lipids (i.e. triglycerides); and
Proteoses—hydrolyse proteins into amino acids.
TheInternational Union ofBiochemistry (IUB) initiated
standardsofenzymenomenclaturewhichrecommendthat
enzyme names indicate both the substrate acted upon and
thetypeofreactioncatalyzed.
Section 2 Pharmacognosy
263

Enzymes can be classified by the kind of chemical
reaction catalyzed.
I. Additionorremovalofwater
A. Hydrolases — these include esterases,
carbohydrases, nucleases, deaminases, amidases,
and proteases
B. Hydrasessuchas fumarase, enolase,aconitase
and carbonic anhydrase
II. Transferofelectrons
A. Oxidases
B. Dehydrogenases
III. Transferofaradical
A. Transglycosidasesofmonosaccharides
B. Transphosphorylasesandphosphomutasesofa
phosphate group
C. Transaminasesofaminogroup
D. Transmethylasesofamethylgroup
E. Transacetylasesofanacetylgroup
IV. SplittingorformingaC—Cbond
A. Desmolases
V. Changinggeometryorstructureofamolecule
A. Isomerases
VI. Joining twomolecules throughhydrolysis of
pyrophosphate bond in ATP or other triphosphate
A. Ligases
Protein Drugs with their Description
Protein drugs Description
Gelatin
soft-gelatin capsules
for vitamin E
hard-gelatin capsules
for chloramphenicol and
acetamenophen
Collagen Collagens are a class of albuminoids found abundantly in bones, skin, tendon, cartilage and
Casein Casein is the principal protein found in cow’s milk. It is responsible for the white, opaque
Lectins Lectins are sugar-binding proteins that are highly specific for their sugar moieties. They
Thaumatin Thaumatin is a low-calorie (virtually calorie-free) protein sweetener and flavour modifier.
Section 2 Pharmacognosy
Gelatin is a protein of uniform molecular constitution derived chiefly by the hydrolysis of
collagen. Gelatin is a high grade gelatin in granular form which may be used as a solidifying
agent or may be incorporated into culture media for various uses.
Types of gelatin:
Absorbable gelatin sponge: It is a sterile, white, tough, and finely porous spongy, water
insoluble, and absorbable substance. It is used as a localized anticoagulant
Absorbable gelatin film: Light amber coloured, sterile, non-antigenic thin film invariably
produced from a specially prepared gelatin-formaldehyde solution. It is used for mechanical
means of protection
similar animal tissues
Collagen protein is distinctive because it contains high levels of the non-essential amino
acids. It contains specific amino acids— glycine, proline, hydroxyproline and arginine.
These particular amino acids are necessary for proper function, growth and repair of the
muscle tissues in the body. In essence, collagen protein is the cement that holds everything
together. Not only it holds components of our skeletal— muscular system together, it is also
the primary mortar between the bricks of all of our smooth muscle tissues such as our blood
vessels, digestive tract, heart, gallbladder, kidneys and bladder
appearance of milk in which it is combined with calcium and phosphorus as clusters of
casein molecules, called micelles. The principal use of casein products has been as an
ingredient in foods to enhance their physical (so-called functional) properties, such as
whipping and foaming, water-binding and thickening, emulsification and texture, and to
improve their nutrition. Casein actually represents the phosphoprotein with a composition of
0.85% P and 0.75% S
play a role in biological recognition phenomena involving cells and proteins. They are
mainly used for determining blood groups; and for carrying out erythrocytic polyagglutination
investigative studies, for performing histochemical studies related to either normal and
pathological status, for establishing structural elucidation studies of the carbohydrate
bearing molecules and it is used as tools for studying cell-surface properties in cancer
research
The substance is often used primarily for its flavour-modifying properties and not exclusively
as a sweetener. It is mostly composed of five distinct forms, viz. thaumatins I, II, III, b, and c.
All of them are almost 100,000 times sweeter than sucrose, and do have molecular weights
around 22,000. The sweetness of thaumatin builds very slowly. Perception lasts a long time,
leaving a liquorice-like aftertaste at high usage levels. Thaumatin is highly water soluble,
stable to heating, and stable under acidic conditions. It is also used largely in such products
as: Chewing gums, breath fresheners as it has enormous usage as a potential low-calorie
sweetener
264

Pest and Pest Management
Pestsare injurious to human health and/or farmers
economicefforts. Pesticides aremixtures of chemicals
thatareusedforkilling,Repelling,mitigatingorreducing
pest damage. Pest organism include insects, rodents,
nematodes,fungi, weeds, birds, bacteria, viruses, etc.
which damage the crops and reduce yield.
Classification of Pesticides
Pesticidesmaybeclassiedaccordingto:
a. The target pest species.
b. Their chemical constitution
c. Theirsiteofaction.
ClassicationofPesticides
Aclassication based on the chemicalcomposition or
structureofthepesticideisthemostusefulforanalytical
chemists, e.g.
Chlorinated hydrocarbons and related pesticides:
Hexachlorocyclohexane (HCH) or benzene hexachloride
(BHC), lindane, methoxychlor
Chlorinated phenoxyalkanoic acidherbicides: 2,4-D,
2,4,5-T
Organophosphorus pesticides: Carbophenothion
(carbofenotion), chlorpyrifos and methylchlorpyrifos,
coumaphos (coumafos), demeton, dichlorvos,
dimethoate,ethion,fenchlorphos(fenclofos),malathion,
methyl parathion, parathion
Carbamate insecticides: Carbaryl (carbaril)
Carbamoyl benzimidazoles: Benomyl, carbendazim
Dithiocarbamatefungicides: Ferbam, maneb,nabam,
thiram, zineb, ziram
Amino acid herbicides: Glyphosate
Inorganic pesticides: Aluminium phosphide, calcium
arsenate
Miscellaneous: Bromopropylate, chloropicrin, ethylene
dibromide, ethylene oxide, methyl bromide, sulphur
dioxide
Pesticidesofplantorigin:Tobaccoleafextract,pyrethrum
ower,andpyrethrumextract;derisandlonchocarpus
root and rotenoid.
Classification based on target pest species
Thefollowing diagram explains the classificationof
pesticides based on target organisms.
Pesticide classication based on target organisms
Section 2 Pharmacognosy
265

Classification based on chemical nature
Pesticide classication based on chemical nature
1. Classification Based on Site of Action
Insecticides/acaricidescanbeclassiedonthebasisoftheir
routesofentryintothebodysystemofthetargetpest.They
canbegroupedasfollows:
i. Stomach poisons, ii. contact poisons, iii. systemic poisons,
iv.fumigants.
Stomach poisons
Stomachpoisonsenterthebodyofthepestthroughthe
mouthduringfeedingintothedigestivetractfromwhere
these are absorbed into the systems.
Stomachpoisons are more effective against chewing
insectsandusefulincontrollinginsectswithsiphoning
orspongingtypesofmouthparts(forexample,housey).
Examples:Dieldrin,sulphur,leadarsenate,etc.
Contact poisons
These poisons enter the body directly through the cuticle
bycontactwiththetreatedsurfaceofthefoliage,stem,
etc.
Thesepoisons act on the nervous system ofthe pest.
Thesemay also be applied directly onto thebody of
the pest as a spray or dust, e.g. benzene hexachloride
dichlorodiphenyl trichloroethane, endrin, quinalphos,
carbamates,etc.Someoftheknownpesticidesderived
fromplants also have contact action,e.g. pyrethrum,
rotenone, sabadilla, nicotine, etc.
Systemic poisons
Section 2 Pharmacognosy
Thesepoisonsareappliedontheplants’surfacesuchas
266
thefoliage,greenpartsofthestem,andneartheroots
fromwherethesearetranslocatedintotheplanttissues.
Systemic poisons are more effective against sucking
pests.Theyhaveaselectiveactionwithlittleeffectonthe
predators and parasites directly, unless acting through
thefoodchain.
Translocationofthesepoisonstakesplacemostlythrough
xylemvessels,e.g.demeton-o-methyl,phosphamidon.
monocrotophosphorate, carbofuran, dimethoate,
mevinphos,aldicarb,etc.
2. Fumigants
Thesearevolatilepoisonsandenterthebodyofthepests
through the respiratory system. These are widely used
in controlling stored grain pests.
Alltypesofpestscanbekilledbyfumigantsirrespective
ofthe types of mouthparts provided a gas-tight
atmosphereisensured(i.e.fumigantsarenonselective).E
venfor soil pests such as nematodes, fumigationis
effective, e.g. dichlorvos, hydrogen cyanide, methyl
bromide, paradichlorobenzene, ethylene dichloride,
carbon tetrachloride, naphthalene, nemagon, aluminium
phosphide, etc.
3. Biopesticides/Bioinsecticides
Biopesticidesarecertaintypesofpesticidesderivedfrom
such natural materials as animals, plants, bacteria, and
certain minerals.
Forexample, canolaoilandbakingsodahavepesticidal
applications and are considered biopesticides.

ADVANTAGES OF BIOPESTICIDES
1. Biopesticides are usually inherently less toxic than
conventionalpesticides.
2. Biopesticidesgenerallyaffectonlythetargetpestand
closely-relatedorganisms,incontrasttobroadspectrum,
conventionalpesticides that may affect organisms as
differentasbirds,insects,andmammals.
15. Quantitative Microscopy
3. Biopesticidesoftenareeffectiveinverysmallquantities
andoftendecomposequickly,therebyresultinginlower
exposuresandlargelyavoidingthepollutionproblems
causedby conventional pesticides. Whenused as
acomponent of integrated pest management(IPM)
programs,biopesticidescangreatlydecreasetheuseof
conventionalpesticides,whilecropyieldsremainhigh.
Stomata are minute pores which occur on the epidermal
surfaceofleavesandalso some herbaceous stems.Each
stoma is guarded by two specialised epidermal cells,
called guard cells. These guard cells are also surrounded
by other specialised epidermal cells called subsidiary cells
or accessory cells. These cells also play an important role
duringopeningandclosingofstomata.
1. Diacytic(cross-celled)orcaryophyllaceous:Inthistype,
thestomataremainssurroundedbyapairofsubsidiary
cells whose common wall is at right angles to the guard
cells, e.g. Acanthaceae, Caryophyllaceae.
2.Anisocytic (unequal-celled) or cruciferous: In this,
stomata remains surrounded by three subsidiary cells
ofwhichoneis distinctly smallerthantheothertwo,
e.g.Cruciferae,Solanum,Nicotiana,etc.
3. Anomocytic (irregular-celled) or ranunculaceous:
In this type, the stomata remains surrounded by a
limitednumberofsubsidiarycellswhicharequitealike
the remaining epidermal cells, e.g. Ranunculaceae,
MalvaceaeandPapaveraceae.
4. Paracytic(parallel-celled)orrubiaceous:Inthistype,the
stomata are surrounded by two subsidiary cells which
areparalleltothelongitudinalaxisofporeandguard
cells.
5. Actinocytic:Thesestomataaresurroundedbyfouror
more subsidiary cells, elongated radially to the stomata.
e.g. Araceae, Musaceae and Commelinaceae.
6. Cyclocytic:Thestomataaresurroundedbyfourormore
subsidiary cells arranged in a narrow ring around the
stoma, e.g. Palmae, Pandanus and Cyclanthaceae.
7. Graminaceoustype:Thestomatalguardcellsaredumb-
bell-shaped.Theyaresurroundedbysubsidiarycells
whichare lying parallel to thelong-axis of the pore,
e.g.inthemembersofPoaceaeandCyperaceae.
Section 2 Pharmacognosy
267

Determination of leaf constants
Anumberof leaf measurementsareusedtodistinguish
some closely related species not easily characterized by
general microscopy.
Stomatal number: Stomatal numberis dened as the
averagenumber of stomata per sq mm of epidermis.
Fragmentsof leaf fromthe middle of the lamina were
cleaned with chloral hydrate.
1. Peeloffupperandlowerepidermisseparatelybymeans
ofaforcepsandmountinglycerinewater.
2. Drawa square of known dimensions(1 sq mm) by
meansof a stage micrometer and camera lucida and
countthenumberofstomataonthatarea.
3. Observethetypeofstomatainthesamepreparation.
4. Determinetenandcalculatetheaverage.Observeunder
10X(eyepiece)and40X(objective).
Stomatal index: Stomatal index is the percentage which the
numbersofstomataformtothetotalnumberofepidermal
cells, each stoma being counted as one cell. Whilst stomata
numbervariesconsiderablywiththeageoftheleafand
dueto changes in environmental conditions,stomatal
indexisrelativelyconstantand,therefore,ofdiagnostic
signicanceforagivenspecies.
1. Countthenumberofepidermalcellsandstomatawithin
the square.
2. Determinetenandcalculatetheaverage.
3. Observeunder10X(eyepiece)and40X(objective).
4. Calculate stomatal index by usingthe following
equation.
Stomatalindex(SI)=(S/E+S)×100
Where,
S=numberofstomataperunitarea
E=numberofepidermalcellsinthesameunitarea
Palisaderatio: Palisade ratio is denedas the average
numberofpalisadecellsbeneatheachepidermalcell.
1. Clearpiecesofleafabout2mmsquarebyboilingwith
chloral hydrate solution.
2. Trace epidermal cells and the palisade cells lying below
them by camera lucida.
3. Firstanumberofgroupseachoffourepidermalcells
were traced and their outlines made more conspicuous.
4. Thepalisadecellslyingbeneatheachgroupfocusedand
traced.
5. The palisade cells in each group counted, cells which
weremorethanhalfcoveredbytheepidermalcellswere
alsocounted;thegureobtainedwasdividedby4to
obtainpalisaderatioofthatgroup.
6. Twenty-ve groups from different leaf sampleswere
determinedforthecalculationofrangeandaverage.
7. Observeunder10X(eyepiece)and40X(objective).
Vein-isletnumber: The vein-isletis the minute area of
photosynthetictissueencircledbytheultimatedivisions
oftheconductingstrands.Veinisletnumberisdenedas
thenumberofvein-isletspersqmmoftheleafsurface,
midway between the midrib and the margin.
1. The leaf sample, after soakingin water, was treated
successivelywithsodiumhypochloritetobleach,10%
hydrochloric acid to remove Ca-oxalate and nally
chloral hydrate.
2. Setupcameralucidabymeansofastagemicrometer
thepaperwasdividedintosquaresof1sqmm.
3. In the cleared preparationveins traced in four
continuoussquares,inasquareof2×2mm.
4. Each vein-islet was numbered during counting.The
range and average was determined in 10 setsof 2 ×
2mm area. Observed under 10X(eyepiece) and 4X
(objective).
Veinletterminationnumber: Veinlet termination number
isdefined as the number of veinlet terminations per
sqmmoftheleafsurface.Aveinterminationistheultimate
freeterminationofaveinletorbranchofaveinlet.Veinlet
terminationwascountedinthe same preparation asfor
vein-isletnumber. The totalnumber of vein-isletsand
veinletterminationsinfouradjoiningsquareswasdivided
byfourinorder togetthevaluein1 sq mm.Therange
andaveragewasdeterminedin10setsof2×2mmarea.
Observeunder10X(eyepiece)and4X(objective).
Section 2 Pharmacognosy
268
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