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Activity Crude Drugs
Carminatives Fennel, Coriander, Clove CNS stimulant Strychnine, Brucine, Caffeine Mydriatics Atropine, Homatropine Antiamoebic Ipecac root, Kurchi bark Myotics Physostigmine, Pilocarpine Hypertensive Ephedrine Hypotensive Reserpine, Veratrine Vermifuge Pelletierine Local anesthetic Cocaine Antimalarial Quinine Antiemetic Emetine Muscle relaxant Curare Antispasmodic Papaverine Uterine stimulant Ergometrine Antigout Colchicines Antiasthmatic Ephedra, Lobelia Purgatives Senna, Rhubarb Expectorant Tulsi, Balsam of tolu
Chemical Classification of Crude Drugs
Carbohydrates
Carbohydrates are compounds containing the elements carbon, hydrogen, and oxygen. They are either aldehyde or ketonic alcohols in which hydrogen and oxygen are present in the same ratio as in water.
Glycosides
Glycosides are non reducing substances that, on hydrolysis brought about by reagents or enzymes, yield one or more reducing sugars among the products of hydrolysis. The non­sugar part of the molecule is called the aglycone or genin; the sugar component is called the glycone.
Tannins
Tannins are complex organic, non-nitrogenous derivatives of polyhydroxy benzoic acids.
Volatile Oils
Volatile oils are odorous principles found in various parts of the plant. They are called volatile oils because they are volatile in steam and at higher temperatures evaporate.
Lipids
The term lipid refers to fixed oils, fats, and waxes. They are esters of long-chain fatty acids and alcohols and closely related derivatives. They are stored in seeds, spores, and vegetative perennial organs such as bulbs
Resins and Resin Combinations
The term resins are brittle secretions or exudations of plant tissues, either produced normally or as the result of pathogenic conditions. Resins, as a class, are hard, transparent or translucent brittle substances. They are generally heavier than water (sp. gr. 0.9–1.25).
Examples
Acacia arabica, Astragalus gummifer, Cyamopsis tetragonolobus and Plantago ovata
Examples Anthraquinone Glycosides: Cassia acutifolia, Aloe perryi,
Rheum emodi.
Saponins Glycosides: Glycyrrhiza glabra, Terminalia arjuna Cyanophore Glycosides: Prunus serotina Isothiocyanate Glycosides: Brassica alba Cardiac Glycosides: Strophanthus Kombe, Digitalis
purpurea
Bitter Glycosides: Picraena excelsa, Swertia chirata Examples
Emblica officinalis, Terminalia arjuna, Uncaria gambier , Acacia catechu and Terminilia chebula
Examples
Foeniculum vulgare, Peucedanum graveolens, Carum Carvi
Examples Fixed oils: Ricinus communis, Oleum Olivae, Prunus
amygdalus and Hypoprion brevirostris
Fats: Theobroma cacao Waxes: Apis mellifera, Prunus amygdalus
Examples
Podophyllum hexandrum, Cannabis sativa, Ipomoea purga, Capsicum annuum, Curcuma longa, Ferula foetida, and Zingiber officinalis.
Section 2 Pharmacognosy
Contd...
149
Alkaloids
The term alkaloid can be defined as a plant base. Alkaloids mean “alkali-like,” referring to the basic nature of these plant constituents. They are essentially basic nitrogenous compounds of vegetable origin, possessing some marked physiological action

3. Factors Influencing Quality of Crude Drugs

Examples Pyridine and Piperidine: Lobelia inflata, Nicotiana tabacum Tropane: Theobroma cacao, Atropa belladonna , Datura
metel and Hyoscymus niger
Quinoline: Cinchona calisaya
Isoquinoline: Papaver somniferum, Cephaelis ipecacuanha
Indole: Claviceps purpurea, Rauwolfia serpentina Purine: Tea, coffee Protein: Gelatin, Ficin, Papain Vitamins: Yeast Triterpenes: Rasna, Colocynth
Environmental Conditions:
Plant growth and development are controlled environmental conditions.
The long-term climatic conditions for a region determine the type of vegetation in that region, and regional environmental factors affect growth and development of the plants.
The ecologically important environmental factors affecting plant growth are light, temperature, humidity, rainfall and altitude.
Effect of Altitude on Cultivation of Plants
Plant Altitude (meters)
Saffron Up to 1250 Clove Up to 900 Coffee 1000–2000 Cinnamon 250–1000
Section 2 Pharmacognosy
150
Tea 1000–1500 Camphor 1500–2000
Effect of Climate on Plants
Plant Climate
Pyrethum Dry weather Saffron Cold Camphor and coffee Cannot withstand frost
Composition of Soil with Its Volume
Components Volume
Air 20–30% Soil solution 20–30% Mineral fraction 45% Organic matter 5%
Type of Soil with Its Characteristics
Type of soil Characteristics
Sandy Soil Sandy soil (0.05 to 2 mm) is good for plants. It is granular and consists of rock and mineral particles. The
texture is gritty and sandy soil is formed by the disintegration and weathering of rocks such as limestone, granite, quartz and shale. Sandy soil retains moisture and nutrients.
Silty Soil Silty soil (0.002 to 0.05 mm) is considered to be one of the most fertile of soils. It is composed of minerals
like Quartz and fine organic particles. It is granular like sandy soil but it has more nutrients than sandy soil and it also offers better drainage.
Clay Soil Clay soil (less than 0.002 mm) is formed after years of rock disintegration and weathering. It is also formed
as sedimentary deposits after the rock is weathered, eroded and transported.
Loamy Soil This soil is the ideal for cultivation, consists of sand, silt and clay to some extent. It is considered to be the
perfect soil. The texture is gritty and retains water very easily, yet the drainage is well.
Peaty Soil It is basically formed by the accumulation of dead and decayed organic matter and naturally contains much
more organic matter than most of the soils. It is generally found in marshy areas. Peaty soil is blocked by the acidity of the soil.
Chalky Soil Chalky soil is very alkaline in nature and consists of a large number of stones. Chalky Soil, apart from being
dry also blocks the nutritional elements for the plants like Iron and Magnesium. The fertility of this kind of soil depends on the depth of the soil that is on the bed of chalk. This kind of soil is prone to dryness and in summers it is a poor choice for plantation.
ESSENTIAL PLANT NUTRIENTS
Essential Plant Nutrients
Nutrient supplied by air and water
Non Mineral Primary Macronutrients Secondary
Carbon Nitrogen Calcium Boron, chlorine, manganese Hydrogen Phosphorus Sulfur Iron, zinc, copper Oxygen Potassium Magnesium Molybdenum and selenium
Nutrient supplied by soil system with help of fertilizers
Micronutrients
Macronutrients
PHYTOHORMONES WITH ITS DESCRIPTION
Phytohormones Nature and
Precusor
Auxins
4-chloro indole-3 acetic acid. Phenylacetic acid Synthesized auxins are: Indole-3-butyric acid (IBA). Napthelene acetic acid (NAA).
Occur in cell in
concentration of
–8
–6
10
–10 mol/litre. Tryptophan is the precursor of IAA & Zn is required for synthesis.
Transport Bioassay Site of
Biosynthesis
Basipetal in stem and acropetal in roots
Avena curvature test. Split pea stem curvature test.
IAA transport is cell to cell mainly in the vascular cambium and epidermal cells.
Effects
Induces cell enlargement and stem growth. Enhances cell division by inducing meristematic activity and Cambium cell activity. Vascular tissue differentiation (Xylem and Phloem). Root initiation (stem cuttings) and differentiation (tissue culture). Tropistic responses of shoots and roots to gravity and light. Growth of flower parts & delay fruit ripening. Leaf and fruit abscission (inhibit or promote via ethylene).
Contd...
Section 2 Pharmacognosy
151
Phytohormones Nature and
Gibberellins (GAs) Weakly acidic plant growth hormone
Cytokinins (CKs) Midly basic growth hormones
Kinetin (6-furfuryl aminopurines) 6-benzyl Aminopurine.
Section 2 Pharmacognosy
152
Precusor
The gibberellins (GAs) are a family of compounds based on the entgibberellane structure
CKs are adenine derivatives characterized by an ability to induce cell division. Chemically precursor of CK is Adenine or Adenosine
Transport Bioassay Site of
Biosynthesis
They are probably transported in the phloem and xylem
CK transport is via the xylem from roots to shoots.
Dwarf pea elongation technique. Barley endosperm digestion technique.
Chlorophyll preservation test or delay in senescence test. Tobacco pith culture test.
synthesized from glyceraldehyde­3-phosphate, via isopentenyl diphosphate, in young tissues of the shoot and developing seed
CK biosynthesis is through the biochemical modification of adenine
Effects
Fruit setting and growth. Auxin delays leaf senescence. Auxin delays ripening. Apical dominance. IAA promotes formation of female flowers (feminization).
Enhances seed germination by enhancing amylase synthesis (enzyme production). Overcome Dormancy. GA1 causes hyperelongation of stems by stimulating both cell division and cell elongation. Induction of seed germination. GAs cause stem elongation in response to long days. Fruit setting and growth. GA produces male flower production in cannabis. GA causes etiolation in plants when kept in dark.
It occurs in root tips and developing seeds. Cell division & Cell enlargement is enhanced by CKs. Morphogenesis. CKs delay leaf senescence. CKs may enhance stomatal opening in some species. CK leads to an accumulation of chlorophyll and promotes the conversion of etioplasts into chloroplasts. In turn leads to Chloroplast development. Growth of lateral buds. Cks are quite effective in breaking dormancy of seeds. It counteracts the phenomenon of apical dominance.
Contd...
Phytohormones Nature &
Precusor
Abscisic acid (ABA) Terpene derivatives.
Ethylene CH
= CH
2
Naturally occurring volatile hormone.
2
It is mainly a 15­C sesquiterpenes, act as a inhibitor because it opposes the growth promotion effect of auxins, GA, CKs
It is gaseous hormones.
Transport Bioassay Site of
Biosynthesis
ABA is exported from roots in the xylem and from leaves in the phloem
Ethylene moves by diffusion from its site of synthesis.
Rice seedling growth inhibition test. Inhibition of α-amylase synthesis in barely endosperm.
Triple pea test. Pea stem swelling test.
Biosynthesis of ABA take place through mevalonic acid or xanthophylls.
It is produced from L-methionine. Ethylene is synthesized by most tissues in response to stress like tissue undergoing senescence or ripening.
Effects
Kinetins are reported to play the role in nucleic acid metabolism & protein synthesis. Increases the self life of vegetables & improves yield and quality of fruit.
Induces dormancy in buds. It is also known as Antigibberellin as it counter effects of gibberellins like induction of hydrolases, α-amylase in barely seedlings. it is known as stress hormone and leads to Stomatal closure thus acts as a antitranspirant. ABA inhibits shoot growth. ABA induces storage protein synthesis in seeds. ABA affects the induction and maintenance of some aspects of dormancy in seeds. Increase in ABA in response to wounding induces gene transcription, notably for proteinase inhibitors.
Maintenance of the apical hook in seedlings. Stimulation of numerous defense responses in response to injury or disease. Release from dormancy. Shoot and root growth and differentiation. Adventitious root formation. Leaf and fruit abscission. Flower induction in some plants. Induction of femaleness in dioecious flowers. Flower opening. Flower and leaf senescence. Fruit ripening.
Section 2 Pharmacognosy
153
Miscellaneous Hormones
Polyamines
H2N — (CH2)3 — NH — (CH2)4 — NH
Spermidine
Polyaminesareagroup of aliphaticamines.Themain
compounds are putrescine, spermidine and spermine.
Theyarederivedfromthedecarboxylationoftheamino
acids arginine or ornithine. They are widespread in all
cellsandcanexertregulatorycontrolovergrowthand developmentatmicromolarconcentrations.Polyamines haveawiderangeofeffectsonplantsandappeartobe essentialforplantgrowth,particularlycelldivisionand
normal morphologies. It appears that polyamines are
presentinall cellsratherthanhavinga specicsiteof
synthesis.
2
Effects:
Jasminatesplayanimportantroleinplantdefenseand
inhibit many plant processes such as growth and seed germination.
Theypromotesenescence,abscission,tuberformation, fruitripening,pigmentformationandtendrilcoiling.
JAis essential for male reproductive development of
Arabidopsis.
Salicylic Acid (SA)
Salicylateshavebeenknownforalongtimetobepresent inwillowbark, but have onlyrecentlybeen recognized
as potential regulatory compounds. Salicylic acid is
biosynthesizedfromtheaminoacidphenylalanine.
Brassinolide
Brassinosteroids (BAs)
Brassinosteroidsare plant steroids,typified by the compoundbrassinolidethatwasrstisolatedfromBrassica pollen.Theyproduceeffectsongrowthanddevelopmentat verylowconcentrationsandplayaroleintheendogenous regulationoftheseprocesses.
Effects
Celldivision, cell elongation, vascular differentiation andtheyareneededforfertility
Inhibitionofrootgrowthanddevelopment
Ithelps in promotion of ethylenebiosynthesis and
epinasty.
Jasmonates (JA)
Jasmonates are represented by jasmonic acid (JA) and its
methylester.Jasmonicacidissynthesizedfromlinolenic acid,whilejasmonic acid ismostlikelytheprecursorof
tuberonic acid.
Effects
Salicylic acid plays a main role in the resistance to
pathogensbyinducingtheproductionof‘pathogenesis­relatedproteins’.
SA is the calorigenic substance that causes thermogenesis in Arum owers.
Ithasalso beenreportedtoenhanceowerlongevity,
inhibit ethylene biosynthesis and seed germination,
blockthe wound response, and reverse the effects of
ABA.
Plant anatomy (anatomy—dissection) is the study
ofinternal structure and organization of plants, especiallyoftheirpartsbymeansof dissection and
microscopic examination. In general, plant anatomy
referstostudyofinternalmorphology,pertainingto differenttissues.
Section 2 Pharmacognosy
154
Fig. 2.1: Classication of plant tissues
Monocot Stem and Dicot Stem
Characteristics Monocot Stem Dicot Stem
Definition It is a circular-shaped hollow axial part of the plant. It is the solid cylindrical axial part of a plant. Internodes Hollow. Solid. Transverse section It is differentiated into the epidermis, hypodermis,
and ground substance in TS.
Epidermal hairs Absent Present Hypodermis Non-green. Green. Cortex Less developed and is represented by the
hypodermis.
Endodermis Absent Present Stele The stele of the monocot stem is larger and of an
advanced type. The stele consists of ground tissues and vascular
bundles.
Pericycle Reduced or completely absent. Present completely or partially sclerenchymatous. Vascular bundles Scattered irregularly throughout the ground tissue. Arranged in the form of a broken ring.
The bundles are numerous and of different sizes. The bundles are fewer and mostly uniform in size. oval, conjoint, collateral, closed, and endarch. Wedge-shaped, conjoint, collateral, open, and
The vascular bundles are covered with a sclerenchymatous sheath.
The vascular bundles remain the same throughout the life of the plant.
The xylem bundles are composed of vessels that are arranged in the form of the letter Y.
The xylem contains protoxylem lacunae. The xylem does not have defined protoxylem
The phloem units are smaller in size and lack phloem parenchyma.
Secondary growth Not observed Dicot stems exhibit secondary growth due to
Medullary rays Absent Present
It is differentiated into the epidermis, cortex, and stele.
Well developed and differentiated into hypodermis, endodermis, and general cortex.
The stele in the dicot stem is larger than the cortex and moderately developed.
The stele is differentiated into pericycle, vascular medulla, and medurally rays.
endarch. The bundle sheath is absent.
Older vascular bundles are replaced by newer ones many times throughout the life of the plant.
The xylem bundles contain fewer vessels that are arranged in rows or columns.
lacunae. The phloem units are larger in size and have
phloem parenchyma.
the presence of secondary vascular tissues and periderm formation.
Section 2 Pharmacognosy
155
Monocot and Dicot Leaves
Characteristics Monocot leaves Dicot leaves
Definition Narrow and elongated with parallel venation, Rounded with reticulate venation Shape Narrow, slender, and longer than dicot leaves. Broad and relatively smaller than monocot leaves. Symmetry isobilateral in symmetry. dorsoventral as the upper and lower surfaces of
the leaves are distinguished.
Venation Parallel venations Reticulate venation Stomata Amphistomatic stomata Hypostomatic.stomata Guard cells Dumb-bell shaped. Kidney-shaped. Intercellular spaces Smaller intercellular spaces as the cells are
compactly arranged.
Vascular bundles Small vascular bundles Larger vascular bundles. Epidermis Epidermal cells of monocot leaf have heavy
deposition of silica. Bulliform or motor cells. Bulliform or motor cells are present.
Mesophyll Differentiated into
Spongy mesophyll and palisade mesophyll.
Larger intercellular spaces as the cells are loosely packed.
Epidermal cells of dicot leaf do not have silica deposition.
Not differentiated.
Monocot and Dicot Root
Characteristics Monocot root Dicot root
Definition Fibrous or adventitious roots Dicotyledonous roots are taproots consisting
of a single primary root from which secondary and tertiary roots develop and grow vertically downwards through the soil.
Root system Fibrous or adventitious root system. Tap root system. Primary root Postembryonic development of the roots. Grow throughout the life Cortex The cortex in monocot roots is wide. The cortex in dicot roots is narrow. Endodermis Thicker endodermis Less thick. Passage cells Present Absent Pericycle Forms the lateral roots. Forms the cork cambium and the lateral roots.
Single-layered or double-layered. Single layered.
Cambium Absent Present Vascular bundles The number of vascular bundles is greater than
six (polyarch).
Conjunctive tissue Parenchymatous. Parenchymatous and Sclerenchymatous. Pith Developed and prominent. Less developed or reduced. Secondary growth Takes place Doesnot takes place
The number of vascular bundles is usually between two and six (diarch to hexarch).
Section 2 Pharmacognosy
156
MULTIPLE CHOICE QUESTIONS
1. Name the Father of medicine:
A. Hippocrates B. Galen C. Aristotle D. Dioscorides
2. Select the drug which is not under the class of organized drug?
A. Gums B. Fruits C. Flowers D. Leaves
3. Select the drug which is not underneath the seed class?
A. Stropanthus B. Isapgol C. Nux-vomica D. Digitalis
4. Choose the drug which does not belong to leaves class:
A. Eucalyptus B. turmeric C. Senna D. Digitalis
5. Choose the drug which does not belong to fruit class?
A. Artemisia B. Coriander C. Fennel D. Colocynth
6. Dried latex of the drug is used, excluding?
A. Papain B. Gatta parcha C. Opium D. Balsam
7. Select the drug whose root are not effective?
A. Turmeric B. Ipecacuanha C. Rauwolfia D. Aconite
8. Select the drug whose leaves are not effective:
A. Digitalis B. Senna C. Clove D. Vasaka
9. Entire parts of the following drugs are effective, except:
A. Belladonna B. Clove C. Ephedra D. Ergot
10. Choose the drug, which is not belonging to tannin class?
A. Ashoka B. Pale catechu C. Myrobalan D. Peppermint
11. Choose the drug which does not belong to volatile oil class:
A. Castor oil B. Garlic C. Peppermint D. Clove
12. Choose the drug which does not belong to carbohydrate class?
A. Colophony B. Acacia C. Guar gum D. Agar
13. Select the drug which is not used as cardiotonics:
A. Squill B. Cinchona C. Digitalis D. Stropanthus
14. Select an antihypertensive drug:
A. Digitalis B. Rauwolfia C. Squill D. Stropanthus
15. Select an adrenergic drug:
A. Ephedra B. Pilocarpus C. Physostigma D. belladonna
16. Select CNS depressant:
A. Belladonna B. Hyoscyamine C. Coffee D. Opium
17. Select the drug which is not used as anticancer?
A. Camptotheca B. Curare C. Podophyllum D. Taxus
18. Select the drug which is not used as antirheumatic
A. Colchicum B. Aconite C. Quassia D. Guggul
19. Choose the emetic drug:
A. Isapghul B. Agar C. Ipecac D. Banana
20. Choose the bronchodilator drug:
A. Liquorice B. Tea C. Ipecacuanha D. Vasaka
21. Choose the antimalarial drug:
A. Ashwagandha B. Tulsi C. Ginseng D. Artemisia
22. Choose the statement which is not the function of cell wall of the plant?
A. Check the rate of transpiration B. Provide mechanical support to the organ C. Protect the protoplast D. Give green colour to leaf
23. Alkaloids are ________ type of substances
A. Basic nitrogenous B. Chemical C. Neutral D. Acid
24. Glycosides are condensation products of:
A. glycone + aglycone B. Fats + aglycone C. Protein + aglycone D. Sugar + Protein
25. Tannins give which colour with iron compound:
A. Light pink B. Blue black C. Pale yellow D. Orange
26. Flower bud of drug showing medicinal importance:
A. Fig B. Clove C. Saffron D. Caraway
27. Aril means:
A. Stiff bristle like appendages B. Succulent growth from hilum C. Enlarged funicle D. Outgrowth originating from micropyle
28. Arillode means:
A. Outgrowth from micropyle B. Stiff bristle like appendages C. Enlarged funicle D. Warty outgrowth
Section 2 Pharmacognosy
157
29. Not an example of organized crude drug:
A. Digitalis B. Aloe C. Cinchona D. Clove
30. Not an example of unorganized crude drug:
A. Cinchona B. Agar C. Aloe D. Colophony
CHOOSE THE CORRECT PAIRS
31. Drug Example
A. Vegetable drug i) Honey bees B. Animal drug ii) Arsenic oxide C. Mineral and metal iii) Belladonna
 32. AsdenedbytheEuropeanPharmacopoeia,which
of the following are herbal drugs?
A. Processed plants, plant or animal parts that are
mainly used in the fresh state, or sometimes after drying
B. Mainly unprocessed, whole or ground plants,
algae, fungi or lichen, which are particularly used after drying or sometimes in the fresh state
C. Generally herbal products, prepared by different
methods such as extraction, distillation, extrusion, fractionation, concentration or fermentation
D. Plant species used as therapeutic agents to
influence the functions of the human body
E. Medical herbs used as therapeutic agents to
influence the functions of the human body
33. Inventor Year
A. Hippocrates i) 384-322 BC B. Aristotle ii) 40-80 AD C. Discorides iii) 400-360 BC D. Galen iv) 131-200 AD
 33. PartUsed Example
A. Roots i) Quassia B. Woods ii) Turmeric C. Rhizomes iii) Karachi D. Bark iv) Rauwolfia
 34. PartUsed Example
A. Gums i) Acacia B. Dried extract ii) Gelatin C. Dried juice iii) Kino
 35. PartUsed Example
A. Seeds i) Vasaks B. Leaves ii) Coriander C. Fruits iii) Pyrethrum D. Flowers iv) Isapgol
 36. Chemicalclassication Example
A. Glycosides i) Cinchona B. Alkaloids ii) Digitalis C. Tannins iii) Ashoka D. Carbohydrates iv) Guar gum
 37.Chemicalclassication Example
Section 2 Pharmacognosy
A. Emetics i) Castor oil
158
B. Purgative ii) Cardamom
C. Carminative iii) Cinchona D. Bitters iv) Ipecacuanha
 38. Chemicalclassication Example
A. Expectorant i) Tea B. Antitussive ii) Opium C. Anitexpectorant iii) Starmonium D. Bronchodilator iv) Vasaka
 39. Pharmacologicalaction Example
A. Anticholinergic i) Physostigmina B. Cholinergic ii) Datura C. Adrenergic iii) Opium
40. Drug Epidermal structure
A. Digitalis i) Wavy B. Hyoscyamus ii) Wavy and striated
cuticle
C. Belladonna iii) Striated walled and
polygonal
D. Senna iv) Thick walled beaded
41. Asavas are medicinal preparations made by:
A. Soaking the drug in semisolid form B. Soaking the drug in decoction form C. Soaking the drug in paste form D. Soaking the drug in emulsion form
42. Asavas and aristas are fermented:
A. For removal of alcohol B. For generation of alcohol C. For removal of water D. For liberation of water
 43. DeneLehas:
A. Solid preparations B. Liquid preparation C. Semisolid preparations D. Emulsified preparations
 44. DeneGutikas:
A. Solid preparations B. Liquid preparations C. Semisolid preparations D. Emulsified preparations
 45. DenationofBhasma:
A. Ash obtained by incineration B. Liquid obtained by calcinations C. Semisolid obtained by calcinations D. Emulsion obtained by calcinations
46. Sodhana in preparation of Bhasma is a:
A. Purification process B. Healing process C. Packing process D. None of the above
47. Tailas are:
A. Solid preparations B. Liquid preparations C. Semisolid preparations D. Powdered preparations
 48. PanchakarmainAyurvedaisadoptedfor
A. Cleaning of the body B. Warming of the body C. Smoothening of the body D. Five-fold detoxification therapy