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287. Use(s)ofplantagospeciesis/are:
A. Reduce blood glucose B. Increase blood glucose C. Increase blood plasma protein D. All of these
288. Antioxidant activity of plantago is due to:
A. Polysaccharide B. Alkaloid C. Pectin D. Flavinoid
 289. Phenoliccompoundsofplantagoworkas:
A. Anti-UV radiation B. Anti-infrared radiation C. Anti-electromagnetic wave D. All of the above
290. Medicinal use(s) of plantago seed is/are:
A. Laxatives B. Elixirs C. Liniments D. All of these
291. Botanical name of turpentine is:
A. Acasia hybryda B. Fraxinus nigra C. Pinus palustris D. Batula papyrifera
292. Select the shape of leaves of pine tree?
A. Elliptic-shaped B. Needle-shaped C. Linear-shaped D. Deltoid-shaped
293. Which is the exposed part of closed cone of pine?
A. Apophysis B. Epiphysis C. Epicarp D. Endocarp
294. Time required for maturation of seed cones in pine trees is:
A. 1 year B. 4 years C. 2 months D. 2 years
295. Important constituent(s) of turpentine is/are:
A. Pinene B. Indole acetic acid C. Piperine D. Both A and B
296. In which species needle-shaped leaves are absent in fascicles of pine tree?
A. Pinus monophylla B. Pinus palustris C. Pinus lumholtzii D. Pinus leiophylla
297. Turpentine is a chemical constituent in ___________________________.
A. Camphene B. Phenol C. Alcohol D. Ether
 298. Use(s)ofturpentineis/are:
A. Solvent B. Oxidant C. Decomposer D. All of these
299. Turpentine is used in the production of:
A. Disinfectant B. Antibiotic C. Antiseptic D. All of these
300. Choose the correct option solublility of turpentine:
A. Water B. Ether C. Phenol D. All of these
301. Botanical name of black pepper is:
A. Mangifera indica B. Piper nigrum C. Centellaa siatica D. Pisum sativum
302. Choose the fruit type of black pepper:
A. Dried and unripe fruit B. Ripe and dried fruit C. It is a drupe fruit D. Both A and C
303. Select the function of body improved by consumption of black pepper:
A. Respiration B. Reproduction C. Digestion D. Urinary system
304. Choose the correct option on size of the fruit of black pepper.
A. 10 mm B. 5 mm C. 5 inches D. 5 cm
305. Select the types of roots present in pepper plant.
A. Aerial roots B. Haustorial roots C. Epiphytic roots D. None of the above
306. On maturity, mostly how many seeds the fruit of black pepper contains?
A. Two seeds B. Single seed C. More than 5 seeds D. Sometimes 4 seeds and sometimes 6 seeds
307. Main constituent(s) of black pepper is:
A. Piperine B. Omega 3 fatty acids C. Triglycerides D. L-citronellol
308. Molecular formula of piperine is:
A. C
10H15NO3
C. C15H15NO3 D. C2H5NO
B. C17H19NO
3
3
309. Choose medicinal use(s) of black pepper.
A. Rubefacient B. Antioxidant C. Antipyretic D. All of these
310. Select the concentration of active constituent piperine in black pepper.
A. 15–18% B. 4–5% C. 10–11% D. 6–9%
Section 2 Pharmacognosy
259
ANSWER KEY
1. D 2. C 3. B 4. B 5. C 6. D 7. B 8. C 9. D 10. A 11. A 12. C 13. B 14. D
15. B 16. C 17. C 18. C 19. B 20. B 21. D 22. D 23. B 24. B 25. D 26. D 27. D 28. B
29. D 30. D 31. A 32. D 33. B 34. A 35. C 36. D 37. D 38. A 39. C 40. A 41. A 42. B
43. C 44. C 45. C 46. D 47. C 48. B 49. D 50. D 51. A 52. B 53. B 54. B 55. D 56. D
57. D 58. B 59. B 60. A 61. A 62. B 63. C 64. B 65. A 66. D 67. B 68. D 69. A 70. A
71. D 72. D 73. C 74. A 75. D 76. A 77. D 78. B 79. B 80. B 81. A 82. D 83. B 84. B
85. A 86. D 87. D 88. A 89. B 90. D 91. D 92. A 93. B 94. B 95. B 96. A 97. B 98. D
99. A 100. D 101. D 102. C 103. A 104. D 105. C 106. D 107. D 108. D 109. C 110. D 111. A 112. A
113. B 114. B 115. D 116. D 117. D 118. D 119. D 120. B 121. D 122. D 123. B 124. B 125. D 126. D
127. D 128. D 129. B 130. A 131. D 132. B 133. B 134. A 135. D 136. C 137. D 138. D 139. B 140. D
141. D 142. D 143. C 144. B 145. D 146. D 147. D 148. C 149. D 150. D 151. B 152. A 153. C 154. B
155. A 156. D 157. D 158. A 159. D 160. D 161. C 162. B 163. D 164. A 165. C 166. B 167. A 168. D
169. B 170. D 171. D 172. B 173. D 174. B 175. D 176. D 177. B 178. D 179. C 180. B 181. D 182. A
183. B 184. A 185. D 186. D 187. D 188. C 189. A 190. C 191. D 192. C 193. C 194. C 195. D 196. D
197. D 198. D 199. D 200. B 201. A 202. C 203. B 204. C 205. D 206. C 207. D 208. C 209. C 210. D
211. D 212. D 213. B 214. D 215. D 216. D 217. D 218. C 219. A 220. C 221. A 222. A 223. C 224. B
225. C 226. A 227. A 228. D 229. D 230. B 231. D 232. B 233. A 234. D 235. A 236. D 237. D 238. D
239. B 240. D 241. A 242. C 243. B 244. C 245. C 246. A 247. D 248. D 249. C 250. A 251. D 252. C
253. D 254. D 255. D 256. D 257. C 258. D 259. D 260. B 261. D 262. D 263. D 264. D 265. C 266. D
267. C 268. C 269. D 270. D 271. A 272. A 273. C 274. D 275. A 276. D 277. D 278. C 279. D 280. C
281. A 282. B 283. A 284. A 285. D 286. D 287. A 288. D 289. A 290. A 291. C 292. B 293. A 294. D
295. A 296. A 297. A 298. A 299. C 300. B 301. B 302. D 303. C 304. B 305. A 306. B 307. A 308. B
309. D 310. D
Section 2 Pharmacognosy
260

12. Toxic Drugs

Common Toxins with their Examples and Effects
Common toxins Examples Effects
Alkaloids Solanine, tomatine 20 mg/100 g of potato and 36 mg/100 g of
tomatoes is unfit to eat
Enzyme inhibitors Protease inhibitors Inhibit trypsin, chymotrypsin, and other protein
digesting enzymes
Saponins Disrupt red blood cells, cause diarrhoea, vomiting
and It may reduce blood serum cholesterol
Cyanogenic glycosides, dhurrin and amygdalin, almonds (bitter almonds), sorghum, choke and pin cherries, and pits of apple, apricot, cherry, plum
Plant phenolics Safrole and coumarin Quite toxic and have been
Tannins Bananas, grapes, raisins, sorghum,
spinach, red wine, beer
Fats, sugars, proteins, and vitamin antagonists
Rancid or oxidized fat erucic acid (mustard, rapeseed) Sugars, proteins and vitamin antagonists
12 bitter almonds can kill a child.
banned Cause of enzymatic browing, bind protein,
precipitate protein of epithelium, cause liver damage, inhibit virtually every digestive enzyme
Liver degeneration and kidney nephrosis headache, dizziness, and cramps facial flushing, rapid heart rate, increased blood pressure, and headaches Tumor promoters

13. Poisonous Plants

Plant Producing Systemic Poisoning
Family Examples
Berberidaceae Podophyllum Euphorbiaceae Euphorbia, Ricinus and Jatropha Leguminosae Cassia, Abrus and Gymnocladus Ginkgoaceae Ginko Apocynaceae Thevetia, Acokanthera Liliaceae Allium, Aloe, Scilla and Urginea Solanaceae Atropa, Capsicum, Datura, Hyoscyamus and Nicotiana Taxaceae Taxus Loganiaceae Strychnos and Gelsemium Meliaceae Melia and Swietenia Umbelliferae Conium, Oenanthe Polygonaceae Rheum Scrophulariaceae Digitalis Rosaceae Prunus, Malus and Eriobotrya
Section 2 Pharmacognosy
261
List of Poisonous Plants with their Description
Poisoning of plants with
Anticholinergic poisons
Calcium oxalate crystals
Cardioactive steroids/cardiac glycosides
Convulsant poisons (seizure)
Cyanogenic compounds
Gastrointestinal toxins
Section 2 Pharmacognosy
Examples Toxins’ mechanism Clinical manifestations Specific therapeutics
Atropa Datura Hyoscyamus Solanum
Competitive antagonism of acetylcholine at the muscarinic subtype of the acetylcholine receptor
Dry, warm, and ushed
skin
Garbled speech
Sinus tachycardia
Urinary retention
Delirium with
It includes sedation with a benzodiazepine or reversal of their clinical syndrome with physostigmine
hallucinations
Brassaia Caladium Caryota Philodendron
Calcium oxalate crystals penetrate the mucous membranes and induce the release of histamine and other inflammatory mediators
After biting or chewing
Local oropharyngeal pain, local swelling garbled speech
After swallowing
Inammation of the
Demulcents, viscous lidocaine, analgesics or with copious irrigation
posterior oropharynx
Oropharyngeal edema
Airway compromise
Adonis Calotropis Digitalis Thevetia
Indirectly increase intracellular Ca concentrations in myocardial cells.
Enhance cardiac
+2
induce vomiting which serves both as an early sign of toxicity to limit poisoning
Abdominal pain and
Antigen-binding regions (Fab) of animal-derived antidigoxin antibodies
inotropy (contractility) and slowdown the heart rate
Caulophyllum Lobelia Myoporum Nicotiana
Seizures including antagonism of gamma­aminobutyric acid (GABA) imbalance of acetylcholine homeostasis, excitatory amino acid mimicry, sodium
Tonic-clonic convulsions,
Loss of consciousness as a result of central nervous system dysfunction, urinary or fecal incontinence
Tongue biting
Anticonvulsant benzodiazepine or lorazepam, should be administered parenterally for persistent seizures
channel alteration, or hypoglycemia
Eriobotrya Hydrangea Malus Prunus
Cyanide inhibits the final step of the mitochondrial electron transport chain, resulting rapidly in cellular energy failure
Abdominal pain, vomiting, lethargy, and sweating, altered mental status, seizures, cardiovascular collapse, and multisystem
Aggressive supportive care, intravenous fluid therapy, intravenous sodium bicarbonate and antidotal therapy
organ failure
Irritant toxins indirectly stimulate contraction of the gastrointestinal smooth muscle. Hepatotoxins may directly injure the liver cells, commonly through the production of oxidant
Nausea, vomiting, abdominal cramping, and diarrhoea
Vomiting may be mitigated by antiemetic agents such as metoclopramide; occasionally, resistant emesis may require a serotonin antagonist such as ondansetron
metabolites
(Contd.)
262
Poisoning of plants with
Mitotic inhibitors Catharanthus
Nicotine-like alkaloids
Pyrrolizidine alkaloids
Examples Toxins’ mechanism Clinical manifestations Specific therapeutics
Colchicum Gloriosa Podophyllum
Caulophyllum Conium Gymnocladus Lobelia Nicotiana
Echium Heliotropium Senecio
These agents interfere with the polymerization of microtubules, which must polymerize for mitosis to occur, leading to metaphase arrest
These agents are direct­acting agonists at the nicotinic subtype of the acetylcholine receptor in the ganglia of both the parasympathetic and sympathetic limbs of the autonomic nervous system
Pyrrolizidine alkaloids are metabolized to pyrroles, which are alkylating agents that injure the endothelium of the hepatic sinusoids or pulmonary vasculature
Gastrointestinal abnormalities, including vomiting and diarrhoea. Nervous system toxicity, including ataxia, headache, seizures, and encephalopathy
Sympathetic stimulation, including hypertension, tachycardia, and diaphoresis, and parasympathetic stimulation, including salivation and vomiting
Acute hepatotoxicity caused by massive pyrrolizidine alkaloid exposure produces gastrointestinal symptoms, right upper quadrant abdominal pain, hepatosplenomegaly, and jaundice as well as biochemical abnormalities consistent with hepatic necrosis
Initial management includes aggressive supportive and symptomatic care
Antihypertensive drugs, including nitroprusside or diltiazem, seizures should respond to intravenous benzodiazepine, such as lorazepam or diazepam
Standard supportive care, liver transplantation

14. Enzymes

Types of Enzymes with Example
Type of enzymes Example
Animal enzymes Lipases, rennets, tripsin Higher-plant enzymes Amylases, papain, proteases, and soya bean lipoxygenase Microorganisms Acetobacter lacti, Clostridium aceticum Food and beverage enzymes Papain, protease
Classicationoftheenzymes based on their site of action:
a. Endoenzymes (intracellular enzymes): The enzymes
thataresolelysecreted verymuchwithinthecellare termed endoenzymes. Theyessentially are involved inthe apt synthesis ofdifferent cellular components, foodreserves,andalsoserveasbioenergeticmaterials. Importantly, as thesevarious processes do occur in theintracellular zones,the enzymes involved are
also strategically located in the intracellular region, e.g. isomerases, phosphorylases, synthetases, etc.
b. Exoenzymes (extracellular enzymes): The enzymes that
areexclusivelysecretedoutsidethecellareinvariably
known as exoenzymes or extracellular enzymes. They
usuallyexertadigestivefeatureintheiroverallactivity andfunction.Interestingly,theyhelpinthehydrolysis ofrelatively complex molecules into much simpler compoundsfortheformationofangiotensinIIsolely
required to maintain and raise the blood pressure.
Examples: Amylases—hydrolyse starch components;
 Lypases—hydrolyse lipids (i.e. triglycerides); and  Proteoses—hydrolyse proteins into amino acids.
TheInternational Union ofBiochemistry (IUB) initiated standardsofenzymenomenclaturewhichrecommendthat
enzyme names indicate both the substrate acted upon and
thetypeofreactioncatalyzed.
Section 2 Pharmacognosy
263
Enzymes can be classified by the kind of chemical reaction catalyzed.
I. Additionorremovalofwater
A. Hydrolases — these include esterases,
carbohydrases, nucleases, deaminases, amidases, and proteases
B. Hydrasessuchas fumarase, enolase,aconitase
and carbonic anhydrase
II. Transferofelectrons
A. Oxidases
B. Dehydrogenases
III. Transferofaradical
A. Transglycosidasesofmonosaccharides
B. Transphosphorylasesandphosphomutasesofa
phosphate group
C. Transaminasesofaminogroup D. Transmethylasesofamethylgroup E. Transacetylasesofanacetylgroup
IV. SplittingorformingaC—Cbond
A. Desmolases
V. Changinggeometryorstructureofamolecule
A. Isomerases
VI. Joining twomolecules throughhydrolysis of
pyrophosphate bond in ATP or other triphosphate
A. Ligases
Protein Drugs with their Description
Protein drugs Description
Gelatin soft-gelatin capsules
for vitamin E
hard-gelatin capsules
for chloramphenicol and acetamenophen
Collagen Collagens are a class of albuminoids found abundantly in bones, skin, tendon, cartilage and
Casein Casein is the principal protein found in cow’s milk. It is responsible for the white, opaque
Lectins Lectins are sugar-binding proteins that are highly specific for their sugar moieties. They
Thaumatin Thaumatin is a low-calorie (virtually calorie-free) protein sweetener and flavour modifier.
Section 2 Pharmacognosy
Gelatin is a protein of uniform molecular constitution derived chiefly by the hydrolysis of collagen. Gelatin is a high grade gelatin in granular form which may be used as a solidifying agent or may be incorporated into culture media for various uses.
Types of gelatin: Absorbable gelatin sponge: It is a sterile, white, tough, and finely porous spongy, water
insoluble, and absorbable substance. It is used as a localized anticoagulant
Absorbable gelatin film: Light amber coloured, sterile, non-antigenic thin film invariably
produced from a specially prepared gelatin-formaldehyde solution. It is used for mechanical means of protection
similar animal tissues Collagen protein is distinctive because it contains high levels of the non-essential amino acids. It contains specific amino acids— glycine, proline, hydroxyproline and arginine. These particular amino acids are necessary for proper function, growth and repair of the muscle tissues in the body. In essence, collagen protein is the cement that holds everything together. Not only it holds components of our skeletal— muscular system together, it is also the primary mortar between the bricks of all of our smooth muscle tissues such as our blood vessels, digestive tract, heart, gallbladder, kidneys and bladder
appearance of milk in which it is combined with calcium and phosphorus as clusters of casein molecules, called micelles. The principal use of casein products has been as an ingredient in foods to enhance their physical (so-called functional) properties, such as whipping and foaming, water-binding and thickening, emulsification and texture, and to improve their nutrition. Casein actually represents the phosphoprotein with a composition of
0.85% P and 0.75% S
play a role in biological recognition phenomena involving cells and proteins. They are mainly used for determining blood groups; and for carrying out erythrocytic polyagglutination investigative studies, for performing histochemical studies related to either normal and pathological status, for establishing structural elucidation studies of the carbohydrate bearing molecules and it is used as tools for studying cell-surface properties in cancer research
The substance is often used primarily for its flavour-modifying properties and not exclusively as a sweetener. It is mostly composed of five distinct forms, viz. thaumatins I, II, III, b, and c. All of them are almost 100,000 times sweeter than sucrose, and do have molecular weights around 22,000. The sweetness of thaumatin builds very slowly. Perception lasts a long time, leaving a liquorice-like aftertaste at high usage levels. Thaumatin is highly water soluble, stable to heating, and stable under acidic conditions. It is also used largely in such products as: Chewing gums, breath fresheners as it has enormous usage as a potential low-calorie sweetener
264
Pest and Pest Management
Pestsare injurious to human health and/or farmers economicefforts. Pesticides aremixtures of chemicals thatareusedforkilling,Repelling,mitigatingorreducing
pest damage. Pest organism include insects, rodents,
nematodes,fungi, weeds, birds, bacteria, viruses, etc.
which damage the crops and reduce yield.
Classification of Pesticides
Pesticidesmaybeclassiedaccordingto:
a. The target pest species. b. Their chemical constitution
c. Theirsiteofaction.
ClassicationofPesticides Aclassication based on the chemicalcomposition or structureofthepesticideisthemostusefulforanalytical
chemists, e.g.
Chlorinated hydrocarbons and related pesticides: Hexachlorocyclohexane (HCH) or benzene hexachloride (BHC), lindane, methoxychlor
Chlorinated phenoxyalkanoic acidherbicides: 2,4-D, 2,4,5-T
Organophosphorus pesticides: Carbophenothion
(carbofenotion), chlorpyrifos and methylchlorpyrifos, coumaphos (coumafos), demeton, dichlorvos, dimethoate,ethion,fenchlorphos(fenclofos),malathion,
methyl parathion, parathion
Carbamate insecticides: Carbaryl (carbaril)
Carbamoyl benzimidazoles: Benomyl, carbendazim
Dithiocarbamatefungicides: Ferbam, maneb,nabam,
thiram, zineb, ziram
Amino acid herbicides: Glyphosate
Inorganic pesticides: Aluminium phosphide, calcium arsenate
Miscellaneous: Bromopropylate, chloropicrin, ethylene dibromide, ethylene oxide, methyl bromide, sulphur dioxide
Pesticidesofplantorigin:Tobaccoleafextract,pyrethrum ower,andpyrethrumextract;derisandlonchocarpus
root and rotenoid.
Classification based on target pest species
Thefollowing diagram explains the classificationof
pesticides based on target organisms.
Pesticide classication based on target organisms
Section 2 Pharmacognosy
265
Classification based on chemical nature
Pesticide classication based on chemical nature
1. Classification Based on Site of Action
Insecticides/acaricidescanbeclassiedonthebasisoftheir routesofentryintothebodysystemofthetargetpest.They canbegroupedasfollows:
i. Stomach poisons, ii. contact poisons, iii. systemic poisons,
iv.fumigants.
Stomach poisons
Stomachpoisonsenterthebodyofthepestthroughthe mouthduringfeedingintothedigestivetractfromwhere
these are absorbed into the systems.
Stomachpoisons are more effective against chewing insectsandusefulincontrollinginsectswithsiphoning orspongingtypesofmouthparts(forexample,housey). Examples:Dieldrin,sulphur,leadarsenate,etc.
Contact poisons
These poisons enter the body directly through the cuticle
bycontactwiththetreatedsurfaceofthefoliage,stem,
etc.
Thesepoisons act on the nervous system ofthe pest. Thesemay also be applied directly onto thebody of
the pest as a spray or dust, e.g. benzene hexachloride dichlorodiphenyl trichloroethane, endrin, quinalphos,
carbamates,etc.Someoftheknownpesticidesderived fromplants also have contact action,e.g. pyrethrum,
rotenone, sabadilla, nicotine, etc.
Systemic poisons
Section 2 Pharmacognosy
Thesepoisonsareappliedontheplants’surfacesuchas
266
thefoliage,greenpartsofthestem,andneartheroots fromwherethesearetranslocatedintotheplanttissues.
Systemic poisons are more effective against sucking pests.Theyhaveaselectiveactionwithlittleeffectonthe
predators and parasites directly, unless acting through
thefoodchain.
Translocationofthesepoisonstakesplacemostlythrough xylemvessels,e.g.demeton-o-methyl,phosphamidon. monocrotophosphorate, carbofuran, dimethoate, mevinphos,aldicarb,etc.
2. Fumigants
Thesearevolatilepoisonsandenterthebodyofthepests
through the respiratory system. These are widely used in controlling stored grain pests.
Alltypesofpestscanbekilledbyfumigantsirrespective ofthe types of mouthparts provided a gas-tight atmosphereisensured(i.e.fumigantsarenonselective).E
venfor soil pests such as nematodes, fumigationis effective, e.g. dichlorvos, hydrogen cyanide, methyl
bromide, paradichlorobenzene, ethylene dichloride, carbon tetrachloride, naphthalene, nemagon, aluminium phosphide, etc.
3. Biopesticides/Bioinsecticides
Biopesticidesarecertaintypesofpesticidesderivedfrom
such natural materials as animals, plants, bacteria, and certain minerals.
Forexample, canolaoilandbakingsodahavepesticidal
applications and are considered biopesticides.
ADVANTAGES OF BIOPESTICIDES
1. Biopesticides are usually inherently less toxic than
conventionalpesticides.
2. Biopesticidesgenerallyaffectonlythetargetpestand closely-relatedorganisms,incontrasttobroadspectrum, conventionalpesticides that may affect organisms as differentasbirds,insects,andmammals.

15. Quantitative Microscopy

3. Biopesticidesoftenareeffectiveinverysmallquantities andoftendecomposequickly,therebyresultinginlower exposuresandlargelyavoidingthepollutionproblems causedby conventional pesticides.  Whenused as acomponent of integrated pest management(IPM) programs,biopesticidescangreatlydecreasetheuseof conventionalpesticides,whilecropyieldsremainhigh.
Stomata are minute pores which occur on the epidermal
surfaceofleavesandalso some herbaceous stems.Each
stoma is guarded by two specialised epidermal cells, called guard cells. These guard cells are also surrounded by other specialised epidermal cells called subsidiary cells or accessory cells. These cells also play an important role
duringopeningandclosingofstomata.
1. Diacytic(cross-celled)orcaryophyllaceous:Inthistype, thestomataremainssurroundedbyapairofsubsidiary
cells whose common wall is at right angles to the guard cells, e.g. Acanthaceae, Caryophyllaceae.
2.Anisocytic (unequal-celled) or cruciferous: In this,
stomata remains surrounded by three subsidiary cells
ofwhichoneis distinctly smallerthantheothertwo, e.g.Cruciferae,Solanum,Nicotiana,etc.
3. Anomocytic (irregular-celled) or ranunculaceous:
In this type, the stomata remains surrounded by a
limitednumberofsubsidiarycellswhicharequitealike
the remaining epidermal cells, e.g. Ranunculaceae,
MalvaceaeandPapaveraceae.
4. Paracytic(parallel-celled)orrubiaceous:Inthistype,the
stomata are surrounded by two subsidiary cells which
areparalleltothelongitudinalaxisofporeandguard
cells.
5. Actinocytic:Thesestomataaresurroundedbyfouror
more subsidiary cells, elongated radially to the stomata. e.g. Araceae, Musaceae and Commelinaceae.
6. Cyclocytic:Thestomataaresurroundedbyfourormore
subsidiary cells arranged in a narrow ring around the stoma, e.g. Palmae, Pandanus and Cyclanthaceae.
7. Graminaceoustype:Thestomatalguardcellsaredumb- bell-shaped.Theyaresurroundedbysubsidiarycells whichare lying parallel to thelong-axis of the pore, e.g.inthemembersofPoaceaeandCyperaceae.
Section 2 Pharmacognosy
267
Determination of leaf constants
Anumberof leaf measurementsareusedtodistinguish
some closely related species not easily characterized by general microscopy.
Stomatal number: Stomatal numberis dened as the
averagenumber of stomata per sq mm of epidermis. Fragmentsof leaf fromthe middle of the lamina were
cleaned with chloral hydrate.
1. Peeloffupperandlowerepidermisseparatelybymeans ofaforcepsandmountinglycerinewater.
2. Drawa square of known dimensions(1 sq mm) by
meansof a stage micrometer and camera lucida and countthenumberofstomataonthatarea.
3. Observethetypeofstomatainthesamepreparation.
4. Determinetenandcalculatetheaverage.Observeunder
10X(eyepiece)and40X(objective).
Stomatal index: Stomatal index is the percentage which the
numbersofstomataformtothetotalnumberofepidermal
cells, each stoma being counted as one cell. Whilst stomata
numbervariesconsiderablywiththeageoftheleafand dueto changes in environmental conditions,stomatal indexisrelativelyconstantand,therefore,ofdiagnostic signicanceforagivenspecies.
1. Countthenumberofepidermalcellsandstomatawithin
the square.
2. Determinetenandcalculatetheaverage.
3. Observeunder10X(eyepiece)and40X(objective).
4. Calculate stomatal index by usingthe following
equation.
 Stomatalindex(SI)=(S/E+S)×100
Where,
 S=numberofstomataperunitarea  E=numberofepidermalcellsinthesameunitarea
Palisaderatio: Palisade ratio is denedas the average
numberofpalisadecellsbeneatheachepidermalcell.
1. Clearpiecesofleafabout2mmsquarebyboilingwith
chloral hydrate solution.
2. Trace epidermal cells and the palisade cells lying below
them by camera lucida.
3. Firstanumberofgroupseachoffourepidermalcells
were traced and their outlines made more conspicuous.
4. Thepalisadecellslyingbeneatheachgroupfocusedand
traced.
5. The palisade cells in each group counted, cells which
weremorethanhalfcoveredbytheepidermalcellswere alsocounted;thegureobtainedwasdividedby4to obtainpalisaderatioofthatgroup.
6. Twenty-ve groups from different leaf sampleswere determinedforthecalculationofrangeandaverage.
7. Observeunder10X(eyepiece)and40X(objective).
Vein-isletnumber: The vein-isletis the minute area of
photosynthetictissueencircledbytheultimatedivisions oftheconductingstrands.Veinisletnumberisdenedas thenumberofvein-isletspersqmmoftheleafsurface,
midway between the midrib and the margin.
1. The leaf sample, after soakingin water, was treated successivelywithsodiumhypochloritetobleach,10% hydrochloric acid to remove Ca-oxalate and nally
chloral hydrate.
2. Setupcameralucidabymeansofastagemicrometer thepaperwasdividedintosquaresof1sqmm.
3. In the cleared preparationveins traced in four continuoussquares,inasquareof2×2mm.
4. Each vein-islet was numbered during counting.The range and average was determined in 10 setsof 2 × 2mm area. Observed under 10X(eyepiece) and 4X (objective).
Veinletterminationnumber: Veinlet termination number
isdefined as the number of veinlet terminations per sqmmoftheleafsurface.Aveinterminationistheultimate freeterminationofaveinletorbranchofaveinlet.Veinlet terminationwascountedinthe same preparation asfor vein-isletnumber. The totalnumber of vein-isletsand veinletterminationsinfouradjoiningsquareswasdivided byfourinorder togetthevaluein1 sq mm.Therange andaveragewasdeterminedin10setsof2×2mmarea. Observeunder10X(eyepiece)and4X(objective).
Section 2 Pharmacognosy
268