Добавил:
Sekretar
kiopkiopkiop18@yandex.ru
t.me/Prokururor I Вовсе не секретарь, но почту проверяю
Опубликованный материал нарушает ваши авторские права? Сообщите нам.
Вуз:
Предмет:
Файл:Ординатура / Хирургия / Библиотека им академика М.И. Перельмана / Книга_5847_Библиотеки_им_академика_М_И_Перельмана
.pdf
5 Babchi
https://t.me/medicina_free
67. Zhang, T., Zhong, S., Meng, Y., Deng, W., Hou, L., Wang, Y., et al. (2018). Quantitative
structure-activity relationship for estrogenic avonoids from Psoralea corylifolia. Journal of
Pharmaceutical and Biomedical Analysis, 161, 129–135.
68. Sözen, T., Özışık, L., & Başaran, N. Ç. (2017). An overview and management of osteoporosis.
European Journal of Rheumatology, 4, 46–56.
69. Fu, P.-K., Yang, C.-Y., Tsai, T.-H., & Hsieh, C.-L. (2012). Moutan cortex radicis improves
lipopolysaccharide- induced acute lung injury in rats through anti-inammation. Phytomedicine,
19(13), 1206–1215.
70. Yu, A.X. D., Xu, M.L., Yao, P., Kwan, K.K. L., Liu, Y.X., Duan, R., etal. (2020). Corylin, a
avonoid derived from Psoralea Fructus, induces osteoblastic differentiation via estrogen and
Wnt/β-catenin signaling pathways. The FASEB Journal, 34(3), 4311–4328.
71. Dua, V.K., Kumar, A., Pandey, A.C., & Kumar, S. (2013). Insecticidal and genotoxic activity
of Psoralea corylifolia Linn.(Fabaceae) against Culex quinquefasciatus Say, 1823. Parasites
& Vectors, 6(1), 1–8.
72. Nam, S.W., Baek, J.T., Lee, D.S., Kang, S.B., Ahn, B.M., & Chung, K.W. (2005). A case
of acute cholestatic hepatitis associated with the seeds of Psoralea corylifolia (Boh-Gol-Zhee).
Clinical Toxicology (Philadelphia, Pa.), 43(6), 589–591.
73. Zhang, X., Zhao, W., Wang, Y., Lu, J., & Chen, X. (2016). The chemical constituents and bioactivities of Psoralea corylifolia Linn.: A review. The American Journal of Chinese Medicine,
44(01), 35–60.
74. Hussain, I., Abro, H.A., & Mubarak, N. (2019). Skin pigmentation effects of Psoralea corylifolia: A case study of Vitiligo. Journal of Islamic International Medical College, 14(1), 48–50.
75. Dhaliwal, S., Rybak, I., Ellis, S., Notay, M., Trivedi, M., Burney, W., etal. (2019). Prospective,
randomized, double-blind assessment of topical bakuchiol and retinol for facial photoageing.
The British Journal of Dermatology, 180(2), 289–296.
76. Chaudhuri, R. K., & Bojanowski, K. (2014). Bakuchiol: A retinol-like functional compound revealed by gene expression proling and clinically proven to have anti-aging effects.
International Journal of Cosmetic Science, 36(3), 221–230.
77. Shoji, M., Arakaki, Y., Esumi, T., Kohnomi, S., Yamamoto, C., Suzuki, Y., et al. (2015).
Bakuchiol is a phenolic isoprenoid with novel enantiomer-selective anti-inuenza A virus activity involving Nrf2 activation. The Journal of Biological Chemistry, 290(46), 28001–28017.
78. Alalaiwe, A., Hung, C.-F., Leu, Y.-L., Tahara, K., Chen, H.-H., Hu, K.-Y., etal. (2018). The
active compounds derived from Psoralea corylifolia for photochemotherapy against psoriasislike lesions: The relationship between structure and percutaneous absorption. European
Journal of Pharmaceutical Sciences, 124, 114–126.
79. Jing, H., Wang, S., Wang, M., Fu, W., Zhang, C., & Xu, D. (2017). Isobavachalcone attenuates MPTP-induced Parkinson’s disease in mice by inhibition of microglial activation through
NF-κB pathway. PLoS One, 12(1), e0169560.
80. Panda, H. (1999). Herbs cultivation and medicinal uses. National Institute of Industrial
Research.
81. Lau, K.-M., Wong, J.H., Wu, Y.-O., Cheng, L., Wong, C.-W., To, M.-H., etal. (2014). Antidermatophytic activity of bakuchiol: In vitro mechanistic studies and in vivo tinea pedisinhibiting activity in a guinea pig model. Phytomedicine, 21(7), 942–945.
82. Khare, C. (2004). Encyclopedia of indian medicinal plants: Rational western therapy,
ayurvedic and other traditional usage, botany. Springer.
83. Bankoti, K., Rana, M., & Bharadwaj, M. (2012). Accelerated stability study of herbal capsules. IOSR Journal of Pharmacy, 2(5), 1–6.
84. Qiao, C.F., Han, Q.B., Song, J.Z., Mo, S.F., Kong, L.D., Kung, H.F., etal. (2007). Chemical
ngerprint and quantitative analysis of Fructus Psoraleae by high-performance liquid chromatography. Journal of Separation Science, 30(6), 813–818.
85. Kunwar, R.M., Shrestha, K.P., & Bussmann, R.W. (2010). Traditional herbal medicine in
Far-west Nepal: A pharmacological appraisal. Journal of Ethnobiology and Ethnomedicine,
6(1), 1–18.
121

122
https://t.me/medicina_free
86. Flaws, B., & Sionneau, P. (2001). The treatment of modern Western medical diseases with
Chinese medicine: A textbook & clinical manual. Blue Poppy Enterprises.
87. Sharma, P., Yelne, M., Dennis, T., Joshi, A., & Billore, K. (2000). Database on medicinal
plants used in Ayurveda. Central Council for Research in Ayurveda & Siddha.
88. Gopal, M., Farahana, B., & Pramesh, R. (2001). Effectiveness of herbal medications in the
treatment of acne vulgaris– A pilot study. The Indian Practitioner, 54(10), 723.
89. Bensky, D., Clavey, S., & Stõger, E. (2004). Materia medica (pp.3–6).
90. Chen, J.K., Chen, T.T., & Crampton, L. (2004). Chinese medical herbology and pharmacol-
ogy. Art of Medicine Press City of Industry.
91. Takizawa, T., Mitsumori, K., Takagi, H., Nasu, M., Yasuhara, K., Onodera, H., etal. (2004).
Sequential analysis of testicular lesions and serum hormone levels in rats treated with a
Psoralea corylifolia extract. Food and Chemical Toxicology, 42(1), 1–7.
92. Wang, X.-X., Lv, X., Li, S.-Y., Hou, J., Ning, J., Wang, J.-Y., etal. (2015). Identication and
characterization of naturally occurring inhibitors against UDP-glucuronosyltransferase 1A1in
Fructus Psoraleae (Bu-gu-zhi). Toxicology and Applied Pharmacology, 289(1), 70–78.
93. Teschke, R., & Bahre, R. (2009). Severe hepatotoxicity by Indian Ayurvedic herbal products:
A structured causality assessment. Annals of Hepatology, 8(3), 258–266.
94. Teschke, R., Wolff, A., Frenzel, C., & Schulze, J. (2014). Herbal hepatotoxicity– An update
on traditional Chinese medicine preparations. Alimentary Pharmacology & Therapeutics,
40(1), 32–50.
95. Xu, Q.-X., Xu, W., & Yang, X.-W. (2020). Meroterpenoids from the fruits of Psoralea corylifolia. Tetrahedron, 76(31–32), 131343.
96. Xu, Q.-X., Zhang, Y.-B., Liu, X.-Y., Xu, W., & Yang, X.-W. (2020). Cytotoxic heterodimers
of meroterpene phenol from the fruits of Psoralea corylifolia. Phytochemistry, 176, 112394.
M. Azeem etal.

Chapter 6
https://t.me/medicina_free
Ashwagandha
SadiaJaved, AyeshaNazir, AmeerFawadZahoor, andArwaA.AL-Huqail
6.1 Introduction
Ashwagandha, or Withania somnifera (WS), is an Indian herb. Winter cherry and
ginseng has both been signicant herbs in the indigenous and Ayurvedic medical
traditions for more than 3000years. The classication of plant’s roots is named as
Rasayanas. They are known for fostering wellness and longevity increasing disease
defense and slowing down ageing procedure, restoring life to the body in sickly
situations, strengthening a person’s ability to withstand negative environmental elements and by fostering a sense of mental well-being wellbeing [1]. The plant has
been used to treat senile dementia, ulcers, bacterial infections, liver ailments, venom
toxins, and more recently, aphrodisiacs, adaptogens, liver tonics, and antioxidants.
The use of WS for anxiety, neurological diseases, inammation, hyperlipidemia,
and Parkinson’s disease is supported by clinical trials and animal studies. Because
of its chemo-preventive qualities, WS may be a helpful adjuvant for patients receiving radiation and chemotherapy. Recently, WS has also been utilized to prevent
continuous use of certain psychotropic medicines from leading to the development
of tolerance and dependency [2].
S. Javed (*) · A. Nazir
Department of Biochemistry, Government College University, Faisalabad, Pakistan
e-mail: sadiajaved@gcuf.edu.pk
A. F. Zahoor
Department of Chemistry, Government College University, Faisalabad, Pakistan
A. A. AL-Huqail
Department of Biology, College of Science, Princess Nourah bint Abdulrahman University,
Riyadh, Saudi Arabia
© The Author(s), under exclusive license to Springer Nature Switzerland AG 2023
M. Zia-Ul-Haq etal. (eds.), Essentials of Medicinal and Aromatic Crops,
https://doi.org/10.1007/978-3-031-35403-8_6
123

124
https://t.me/medicina_free
S. Javed etal.
6.2 Taxonomical Classication
Ashwagandha scientically called Withania somnifera is taxonomically classied
as; it belongs to Kingdom Plantae, Plants and subkingdom Tracheobionta, Vascular
plants. The super division is Spermatophyta, Seeds plants. Division Angiosperma,
class Dicotyledons, order Tubiorae, family Solanaceae, genus Withania and
Species somnifera Dunal (Table6.1) [2].
6.3 Botanical Structure
This plant is a small in size. Its branches extend from central stem in the manner of
stars (stellate), and it is covered with a dense matte woolly hair called tomentose.
The fruit is mature when it is orange-red and contains milk-coagulating characteristics, while the blooms are tiny and green. Long, brown, tuberous roots of the plant
are used as a medicine.
6.4 Occurrence
The semi-arid area of India and other Southeast Asian nations is home to the plant
known as Ashwagandha. Additionally, the plant may be found in parts of Africa
including the Congo, South Africa, Egypt, Morocco, and the Middle East [3]. This
plant is also grown in Pakistan and Sri Lanka. It is also called Indian ginseng and it
has been used by Ayurvedic practitioners for thousands of years [4]. Madhya
Pradesh, Haryana, Gujarat, Punjab, Maharashtra, Uttar Pradesh, and Rajasthan are
the Indian regions where most of WS is grown. More than 5000 acres of Madhya
Pradesh are dedicated to its cultivation. With an annual demand of over 7000 tons
and predictable output from its Indian sources of more than 15,000 tons, it is necessary to enhance agricultural production and improve production. Ayurvedic practitioners have used WS for thousands of years a cure for various diseases [4].
Table 6.1 Taxonomical
classication of Withania
sominefera
Kingdom Plantae, plants
Subkingdom Tracheobionta; vascular plants
Super division Spermatophyta, seed plants
Division Angiosperms
Class Dicotyledons
Order Tubiorae
Family Solanacae
Genus Withania
Species sominefera Dunal

6 Ashwagandha
https://t.me/medicina_free
125
6.5 Pests andDiseases
No signicant pests have been identied in the crop. Two or three Neem Astra
sprays as the foliar spray was found to be extremely helpful against aphids, mites,
and insect attacks at 10days per time the crop is harmed by insects. Some diseases
resemble seedling rot and blight, according to reports. High humidity and temperature levels drastically increase seedling mortality. Usage of seeds which are disease
free and proper seed preparation before the process of sowing can lower the likelihood of illness. Neem cake can also be utilized. It will protect nematodes and insects
from root damage. Additionally, by using crop rotation, planting at the right time,
and ensuring appropriate soil drainage, the crop will be protected [4].
In an experimental study, Ashwagandha is affected by a number of insect pests,
such as the Epilachna vigintioctopunctata which is a leaf beetle having spots on it.
By applying Azophos (2kg/ha), decomposed mixture of dung or manure (FYM)
(12.5t/ha) and cake of neem (1000kg/ha), it was discovered that the damage caused
by spotted leaf beetles could be reduced by 69.79%. With a minimal feeding area of
6.75cm
(laying of eggs), which was noted 62.00 eggs per plants Ravikumar, Rajedran,
Chinniah, & Irulandi). The lowest Epilachna beetle infestation and maximum production were seen with dimethoate 30 EC (1ml/l). Endosulfan NSKE ver percent,
Fenvalerate and chlorpyriphos, were potentially effective treatments that were comparable to dimethoate [5, 6]. On W. somnifera, gonim and B. thuringensis were
shown to be the most efcient pesticides against red bugs and aphids also decrease
the larval population of defoliators [7]. 250 LE (63.61) of HaNPV, which was comparable to nimbecideine at 3ml/L (56.66) and then NSKE at 5% (47.42) and oil
extracted from neem at 5ml/l (45.73), recorded the highest mean percent decrease
of H. armigera infestation on Ashwagandha above control [8]. The gram caterpillars, H. armigera, eat the fragile leaves of Ashwagandha plants and bore into the
fruits. As part of an integrated strategy, eld release of T. chilonis (100,000 eggs/
ha), application of HaNPV (250 LE/ha) or B. thuringiensis (0.5kg/ha) and deployment of pheromone traps (12 traps/ha) were advised. Deilephila nerri, a minor defoliator that feeds on hawk moth caterpillars, is also managed by exposing pupae in
the soil, removing and destroying larvae by hand, setting up 1 trap each ha of light
traps, and also setting Nerium oleander (L.) as a trap plant surrounding the eld [9].
2
, Azophos and neem cake combination was not able to reduce oviposition
6.6 Historical Background
The Indian traditional medical system known as Ayurveda traces back to the
6000BC [10]. Historicaly, Ashwagandhaa was used as therapy called Rasayana. Its
root is valued for its thermogenic, narcotic, tonic, anthelmuntic, diuretic, anthelmintic, stimulant and astringent properties.The name “Ashwagandha“refers to the fact
that the root has a horse-like odor. In ancient times it was believed to give horse lie

126
https://t.me/medicina_free
powers when consumed. Emaciation in children (when consumed with milk, it
becomes the excellent source of nutrition for children), leukoderma, old age, debility, constipation, rheumatism sleeplessness, neurological disorders, goiter, etc. are
all ailments for which it is frequently used [11]. Application of WS root paste which
is formed by crushing its roots with water helps to decrease inammation in joints
[12]. Additionally, it is used locally to painful swellings such carbuncles and ulcers
[13]. For both scorpion stings and snake venom, the root is administered in association with other medications. Additionally, it aids in the treatment of piles, worms,
boils, and leucorrhoea [14]. Out all of its varieties available the Nagori Ashwagandha
is the best one. When using fresh Ashwagandha powder, the benets are the maximum [15]. The bitter leaves are suggested for fever and throbbing swellings. The
owers have aphrodisiac, diuretic, astringent, and depurative properties. The seeds
are anthelmintic and erase white spots from the cornea when mixed with an astringent and rock salt. It is used to make Ashwagandharishta, which is used for The
Nagori Ashwagandha is the best among hysteria, syncope, memory loss, anxiety,
etc. Additionally, it stimulates the body and enhances the count of sperm in
males [15].
S. Javed etal.
6.7 Characteristics
Ashwagandha is a perennial to annual, branching shrub with tiny stellate and tomentose branches that grows from 30 to 120cm. The roots are brownish-white, meaty,
and tapered. Oval leaves and greenish blooms are present. Fruits that are orange and
red are mature.
6.7.1 Variety
A variety called “Jawahar” from Madhya Pradesh, is a small in height and best
suited for planting in dense populations. Within 180days, the variety produces dry
roots with a cumulative withanolide concentration of 0.30%.
6.8 Conditions forGrowth
It is grown as a crop during the Kharif (late rainy season). The best places to cultivate crops that are nourished by rainfall are semi-tropical regions with 500–750mm
of annual precipitation. Natural rain enhances the roots increases the growth. This
plant usually requires dry season to grow. Temperatures between 20 and 38°C, as
well as low temperatures as low as 10, can be tolerated. It grows to 1500m of height
above the sea level. Ground and soil.

6 Ashwagandha
https://t.me/medicina_free
Ashwagandha thrives on loam soil or redish soil with 7.5–8.0 of the pH range
and adequate drainage.
127
6.8.1 Land Preparation
Before it rains, two to three plowing, discing, or harrowing operations should be
carried out to get the soil to a very good tilth. For application, mixing, and leveling
of the land, FYM 25 tons per hectare.
6.9 Conservatory
Ashwagandha is grown from seeds. Then freshly taken seeds are taken and sown in
the prepared beds of nursery. While the broadcast method may be used to seed it in
the eld, the transplanting approach is preferable for increased efciency and export
purposes. A well-kept nursery is essential for export. The nursery bed, which is
normally elevated off the ground, is prepared with sand and the compost.
Approximately ve kilograms of seed is needed to plant on 1ha of the eld. The
nursery is built up throughout June and July. The seeds are spread and gently covered with sand just before the monsoon. The seeds begin to sprout after 5–7days.
Older seedlings that are around 35days old are transferred into the main sector.
6.10 Irrigation
Water or excessive rains harms this crop. After transplanting, light rain helps plants
establish more quickly. If necessary, irrigation that can save lives may be offered.
For improved outcomes and a greater root output in an irrigated condition, the crop
might be watered once every 10days.
6.11 Transplanting
After the well incorporation of manure into the soil, ridges are placed 60cm apart.
For seedlings of good quality, the distance is kept of 30cm. In various places, spacing of 60cm×60cm or 45cm×30cm is frequently used. The spacing of 60cm
into 30cm is thought to be the most advantageous with the plant density of roughly
per hectare of 55,000 seedlings.

128
https://t.me/medicina_free
S. Javed etal.
6.12 Sowing andSeeding Rate
For broadcasting techniques the seed rate of 10–12kg each hectare is acceptable.
Alternatively, rows may be planted. The method of line-to-line is recommended
because it promotes the development of roots and aids in the efcient execution of
cross-cultural activities. Typically, the seeds are spread between 1 and 3cm deep. In
all processes, light soil should be applied to the seeds. Maintaining a line to line
distance is kept 20–25cm and a plant to plant distance of 8–10cm is crucial. The
spacing may be changed based on the soil fertility. In marginal soils, the population
is often maintained at higher levels.
6.13 Treatment ofSeed withTrichoderma viride
To protect seedlings against illnesses transmitted by seed, treatment of Trichoderma
viride can give at a rate of 3g/Kg of seed before planting. For protection of seed-
lings from illnesses due to bad seeds, Dithane M45 (Indol M45) or Thiram at a rate
of 3g/Kg of seed for handling.
6.14 Intercultural Practices
Mature seedlings are cultivated sown by hand 25–30days or by the broadcasting
method or after sowing to keep plant density of around 30–60 plants per square
meter. Kind and fertility of the soil can affect the number of plants that can be maintained at prior levels. The population needs to ideally be kept at a lower level if
fertilizer is used. In general, weeding is crucial to keep the eld weeds free for about
initial 25days after planting and subsequent 20–25days.
6.15 Manures andFertilizers
Growth of WS doesn’t require any signicant amounts of manure or fertilizer.
Inorganic fertilizers are rare to be used. The crop responds favorably to compost,
vermin compost, and organic manures. For each ha, it is advised to apply 10t of
FYM or 1t of vermin-compost. The application of 15kg of nitrogen and 15kg of
phosphorus per acre is useful for more output in typical rich ground. For enhanced
root output in rich stumpy soils, 40kg of N and P per hectare is sufcient.

6 Ashwagandha
https://t.me/medicina_free
129
6.16 Reaping
In well-managed crops, Ashwagandha yields dry root of 3–5 q and 50–75 kg of
seeds per hectare in 180days. Under scientic crop management, the dry root production increases to 6.5–7.0 q/ha. Farmers have occasionally gotten root yields as
high as 1t/ha. Commercially, roots with a diameter about 15mm and a length of
7–10cm are chosen. Roots contain an alkaloid content ranging from 0.13% to 0.31%.
6.17 Demand inMarket
Every year, makers of Ayurvedic and Siddha medicines, as well as importers, global
buyers, processors, and herbalists, visit these marketplaces to purchase Ashwagandha
roots. Recognition of these plants therapeutic and nancial benets is growing in
both developing and developed nations. As was already mentioned,there is a 7000ton yearly domestic demand for Ashwagandha roots. The domestic market in India
has a large potential because the output there is substantially lower than the average
of 1500 tons [4].
6.18 Chemical Constituents
The Ashwagandha root and leaf extracts in methanol, hexane, and diethyl ether
were discovered. Roots contain an alkneuroaloid content ranging from 0.13% to
0.31%. The alterative, narcotic, deobstruent, diuretic, restorative, aphrodisiac, sedative and properties of Withania somnifera’s roots are well known. Ateroidal lactones
and Alkaloids are known to be responsible for the root’s therapeutic effect. Although
substantially higher yields (up to 4.3%) have been observed, the alkaloid content of
roots of Indian varieties varies between 0.13 and 0.3. There are a variety of heterogeneous biochemical alkaloids, such as isopelletierine, tropanol, cuscokygrene,
choline, 3-tigioyloxytropana, pseudotopanol, and a number of many other lactons
steroidal in nature. From the plant’s roots, researchers have identied 12 alkaloids,
35 withanolides, and many sitoindosides. Withanolide, a physiologically active
component having a C27 glucose molecule at, is known as sitoindoside. Withaferin
D and withanolide A are the primary withanolides thought to be responsible for
Indian ginseng‘s pharmacological effects. According to reports, leaves contain
withaferin-A, a withanolide that is therapeutically effective. WS roots also contain
starch, dulcitol, glycosides reducing sugars, withancil, also alkaloids, neutral and
substances. Tyrosine, glycine, glutamic acid, aspartic acid, alanine, and cysteine are
among the amino acids identied from the roots [16]. Additionally, this plant contains a high amount of iron as well as chemical constituents like withaniol, starch,
acylsteryl glucosides, ducitol, reducing sugar, hantreacotane and reducing sugar
(Fig.6.1) [17].

130
https://t.me/medicina_free
S. Javed etal.
Fig. 6.1 Chemical constituents of Withania somnifera (WS)
6.19 Biosynthesis ofWithanolides
WS produces withanolides, which are ergostane skeleton-based C28-steroidal lactones produced from triterpenoids. De novo biogenesis and withanolide accumulation are most active in young leaves and begin to wane as leaves mature. In plants,
isoprenoid biosynthesis occurs via two distinct pathways: the cytosolic mevalonate
(MVA) system and the plastid-localized 2-C-methyl-d-erythritol-4-phosphate
(MEP) pathway. Isoprenoids produced by these pathways are channelled into a variety of metabolic pathways, where they create a variety of specialised metabolites
engaged in a variety of cellular and regulatory functions. Condensation of acetyl
CoA molecules with acetoacetyl CoA results in 3-hydroxy-3-methylglutaryl-CoA
(HMG-CoA) and mevalonic acid. HMG-CoAs are irreversibly converted into mevalonic acids by HMGR. 3-isopentenyl pyrophosphate is formed by mevalonate-5pyrophosphate decarboxylase (IPP). FPPS catalyses the condensation process of
IPP with another IPP molecule. The MEP pathway is an important part of withanolides production, involving the conversion of pyruvate into d-glyceraldehyde-3phosphate (DXP), DXP synthase (DXS), MEP (MEP) and
4-diphospho-cytidyl-2-methyl-d-erythritol (CDP-ME) from a CTP-dependent reaction catalyzed by CMS and CMK respectively (Fig.6.2) [18].
Соседние файлы в папке Библиотека им академика М.И. Перельмана
