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Y. Sheena et al.
marks guide the approach and thigh vasculature is less prone
to variation, but it is worth being aware of differences (even
within the same individual on the contralateral side) in the
origin, calibre and course of leg vessels. One important variant being the Peronea Arteria Magna, where one or both of
the Anterior and Posterior Tibial arteries are congenitally
small or absent, making the Fibular artery the dominant
blood supply to the distal leg in 5–9% of patients [1, 2]. It is
wise to note the risks of atherosclerotic occlusive or deep
venous thrombotic disease, and some would argue colour
Doppler ultrasound performed by radiologically trained surgeons gives the most useful dynamic information on the con-
dition of the vessels for microsurgery [3, 4]. In elective
oncological or chronic infection cases, vessels involvement
is worth investigating and in trauma—fractures, penetrating
injuries and external forces may have caused vessel damage
precluding their use or mandating vascular reconstruction
during microsurgery. It is our common practice to utilise
thermal imaging and hand-held Doppler for skin perforator
mapping/ap design, and all our trauma patients have routine
pre- operative CT angiography, which many use as the ‘gold
standard’ investigation [5, 6] to dene the pre-operative vascular state, relationship to orthopaedic injuries and to plan
microsurgical reconstruction (see Fig.49.1).
Fig. 49.1 CT angiograms on the left showing normal bilateral 3-vessel run off; and on the right in an open left tibia and bula fracture with arterial
injury signied by no ow in the proximal 10cm of the anterior tibial and peroneal arteries

49 Lower Limb Recipient Vessels Access
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49.4 Recipient Vessel Access [See Fig.49.2
forLeg Surface Markings]
If appropriate recipient vessels are not available within the
defect to be reconstructed, the principle of access is to connect the defect to the safe fasciotomy lines [7] via the shortest necessary incisions (see Fig. 49.2). The incisions go
through skin, subcutaneous fat, fascia and into the relevant
plane retracting tendons/muscles/nerves to isolate the
selected recipient vessels with the nal preparation performed under the microscope. The most commonly used
recipient vessels are the anterior or posterior tibials. There
are pros/cons of each and we are aware of past schools of
thought preferring the PTA [8], with Godina describing a
mid-axial approach splitting the medial and lateral gastrocnemius muscle [9]. More recent experience shows the ATA
to be just as reliable, even when the vessels are damaged in
the zone of trauma, they have equivalent ap success rates
when dissected proximal to injury [10]. Other not infrequently utilised recipient vessels include the dorsalis pedis,
supercial femoral and its descending genicular branches.
Less commonly utilised ‘get out of jail’ vessels include the
bular, popliteal and vein grafts or AV loops to more proximal thigh vessels. The PTA is commonly dominant in supplying blood distally, so we rarely perform end-to-end
anastomosis on this artery. It has predictable perforators to
surrounding muscles and medial leg skin (described at 5, 10
and 15cm above the medial malleolus) and these are ideal
recipients for free ap microsurgery. We prefer to perform
end-to-side arterial anastomoses to maintain axial limb blood
ow, especially when less than three healthy vessels perfuse
the distal leg or in patients with peripheral vascular disease
[11]. In a ‘one vessel’ leg our preference is to use a ‘owthrough’ free ap to reconstruct the damaged vessels, and
some surgeons believe these may be associated with
improved ap survival [12]. Some cases with gures to illustrate vessel access follow.
483
1. Dorsalis Pedis Vessels [Figs. 49.3 and 49.4]:
Useful for dorsal foot wounds when the vessels are
available. DPA is the continuation of the ATA distal to the
ankle joint and terminates as the First Dorsal Metatarsal
Artery and the Deep Plantar Artery. Its course can be sur-
Fig. 49.2 Image of left leg with subcutaneous Tibial borders (Black
solid lines), fasciotomy election lines (Green dashed) and PTA perforators medially (Red crosses); and Right leg illustrating three potential
open fracture soft tissue defects (Red hatched) with access incisions
(Blue curved lines) in relation to fasciotomy lines. The inferior defect to
ATA, middle 1/3 defect to PTA and proximal defect to DGA or SFA
face landmarked by the line joining the mid-point of the
malleoli and the proximal rst metatarsal interval. Find
the pulse just lateral to Extensor Hallucis Longus (EHL)
and medial to Extensor Digitorum Longus (EDL) most
readily palpable at the distal navicular bony prominence.
During dissection, identify and protect the adjacent deep
peroneal nerve.
2. Anterior Tibial Vessels [Figs. 49.5, 49.6, 49.7, 49.8, 49.9,
and 49.10]:
The surface landmark line of the ATA is described by
a line joining the medial bula head to the dorsal midmalleolar point. Find these vessels distally between the
Tibialis Anterior (TA) and EHL tendons. More proximally these vessels become deeper between the muscles
of TA medially and EDL laterally. Protect the adjacent
deep peroneal nerve during vessel dissection. The images
show a lateral ankle open fracture (xed by diastasis

484
Y. Sheena et al.
Fig. 49.3 Left dorsal foot defect with skin surface markings for access
to the dorsalis pedis vessels
Fig. 49.4 DPA with its two VCs dissected and clear for microsurgery
at site with blue background
Fig. 49.6 Right ATA prepared for microsurgery with its two anking
VCs between tibialis anterior (retracted medially/above) and the EHL/
EDL muscles (retracted laterally/below, along with the deep peroneal
nerve seen distally on right side of wound in image)
Fig. 49.7 Two-perforator ALT ap connected end-to-end on to the
right ATA seen from lateral side
Fig. 49.5 Right lateral ankle defect with surface markings for access
to anterior tibial vessels
Fig. 49.8 ALT ap anastomoses showing two venous couplers end-toend on to ATA VCs

49 Lower Limb Recipient Vessels Access
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Fig. 49.9 ALT ap inset to cover the defect distally and the vessel
access incision/wound proximally
485
Fig. 49.11 Right distal tibial open fracture with IM nail in situ showing access incision to PTA
Fig. 49.10 ALT ap dressings at end of procedure demonstrating
monitoring window and pressure care instructions around anastomosis
site
screw) with a soft tissue defect in a leg with three-vessel
run-off with an access incision towards the distal anterior
tibial vessels allowing an end-to-end arterial hand-sewn
and two venous coupled ALT (double perforator) ap
anastomoses.
3. Posterior Tibial Vessels [Figs. 49.11, 49.12, and 49.13]:
The PTA runs from the end of the PA (2.5 cm below
the mid-Popliteal fossa) to a point between the medial
malleolus and the heel where the pulse should be easily
palpable. Passing under the ankle exor retinaculum the
mneumonic ‘Tom, Dick and Nervous Harry’ serves to
remind of the anterior to posterior relationships of Tibialis
Posterior, Flexor Digitorum Longus, the Tibial Artery,
Tibial Nerve and Flexor Hallucis Longus. In the leg, the
Fig. 49.12 MSAP ap over tibial defect with end-to-side anastomosis
to PTA and one ap vein coupled to its VC and the second to a supercial system vein
Fig. 49.13 MSAP ap inset resurfacing defect. Note proximal half of
ap utilised to cover vessels
PTA travels with its Venae and the Tibial nerve in the
deep posterior compartment (deep to the transverse
intermuscular septum supercial to the Tibialis Posterior
muscle) between the FDL and FHL muscles. It is usually

486
Y. Sheena et al.
approached medially, taking care not to injure the Great
Saphenous Vein (GSV) and Saphenous Nerve, by releasing and retracting the crural fascia and Soleus off the
posterior-medial Tibial border. Care must be taken not to
injure the Tibial nerve. This open Tibial fracture had IM
nail xation and immediate contralateral MSAP free ap
reconstruction. The incision allowed access to the PTA,
its VCs and a supercial vein were also identied, dissected and utilised. An end-to-side arterial and two coupled venous anastomoses (one to deep VC and one to the
supercial recipient). Note that swelling in the acute
trauma setting often requires larger aps be harvested in
anticipation of failure to close the vessel access incision
and to cover the pedicle.
4. Supercial Femoral and Descending Genicular Vessels
[49.14].
These vessels are useful in cases of higher leg, knee or
thigh defects that require reconstruction. The SFA is surface marked by the upper two-thirds of a line from the
mid- inguinal point (midpoint between Pubic Symphasis
Pubis and Anterior Superior Iliac Spine) and the Adductor
Tubercle. As aforementioned, it travels under Sartorius
and can be accessed between Adductor Longus (easily palpable with the relaxed thigh abducted and externally
rotated). The SFA usually gives off a branch medially
called the Descending Genicular Artery (DGA) approximately 13cm above the knee joint, which travels between
Sartorius and Vastus Medialis in close proximity to the
Saphenous nerve. This nerve and the GSV should be identied and protected when preparing these vessels. See
Fig.49.14 for an image showing DGA vessel preparation
to receive an LD free ap to reconstruct a proximal tibial
Gustillo 3B fracture with single vessel distal run off and
poor Popliteal branches on CTA.
49.5 Core Surgical Techniques inRecipient
Vessel Harvest
To summarise:
1. Recipient vessel selection is based on defect location and
the condition of local vessels on CTA.
2. Surface landmarks and hand-held Doppler conrm access
incision placement. Utilise shortest line from defect to
safe fasciotomy lines (see Fig.49.2).
3. Tourniquet control dissection for bloodless eld and
Loupe magnication allow accurate identication of
nerves, supercial veins (that may be utilised during primary or rescue anastomoses).
4. Adequate assistance and good retraction allows safe iden-
tication and control of selected vessels.
5. Final preparation under microscope to conrm satisfac-
tory vessel walls, an arterial ‘squirt test’ and low pressure
venous ‘ush test’ are key requirements.
6. Aim for perforator-to-perforator (when available) end-to-
end anastomosis or an end-to-side anastomosis with more
major recipient vessels. End-to-side anastomosis has
comparable patency and ap outcomes, addresses vessel
size mismatch and preserves distal limb perfusion and
preserves recipient vessel options if subsequent reconstruction or further free ap surgery is ever required.
7. Check for venous backow and low resistance heparin-
ised saline ushing. Anastomosis proximity to valves is
not usually an issue. There must be a low threshold in
connecting an extra vein to the ap, if possible, especially
if turgid second ap vein with rst vein owing, or if high
pressure recipients (we mitigate DVT risk by utilising a
supercial second vein recipient if available).
8. Assess immediate ow with Acland test, note darker
blood return to vein on clamps down and usual ap observations noting colour of bleeding from dermal edges with
low threshold to revision/additional vein anastomoses or
Vein Grafts if required.
Fig. 49.14 Medial thigh access incision showing the DGA utilised as
recipient vessel for proximal tibial reconstruction
49.6 Pearls andPitfalls
Pearls
• Utilise pre-operative imaging—Angiography or
Colour Doppler Ultrasound (CDU).
• Identify/protect any supercial veins around defect
for reconstruction. We aim to anastomose two veins if
the ap pedicle has two VCs. Utilising a recipient site
VC and a supercial vein mitigates the risks of relying on just the deep or supercial venous systems.

49 Lower Limb Recipient Vessels Access
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487
• Assess vein wall condition, blood backow on cutting and ease of ushing with heparinised saline.
• Careful ap templating taking extra skin to account
for and cover the vessels and access incision.
• Arterial assessment considers non-contused appearance, visible/palpable pulsatility (we use intraoperative pencil Doppler) and most importantly the
‘squirt test’ to conrm adequate ow pressure.
After microsurgery conrm vessels not twisted,
kinked, or under tension/pressure. We sometimes
utilise a small piece of fat to help cushion the pedicle around the anastomosis site.
Pitfalls
• Regardless of microsurgical planning and execution, the adequacy of excision margins will determine outcome. It is complete oncological clearance
or radical wound excision to healthy tissues in the
infective or trauma setting that will minimise cancer recurrence, infection and non-union. It is crucial
for the microsurgeon to perform this well or work
closely with the ablative surgeon to do so.
• Any factor from the pre- to the post-operative
course can compromise the vessels, ap and entire
reconstruction. The adequacy of your local preoperative clinical and radiological assessment helps
guide the operative vessel selection and it is well
worth learning to correlate your local angiography
procedure (for DSA, CTA, MRA, etc.) with operative ndings.
• Adequate arteries are necessary, but not sufcient
for ap success. Venous congestion is the greater
risk so identifying injuries or deep vein thrombosis
preoperatively can help plan to mitigate ap venous
compromise. Colour Doppler ultrasound may have
an imaging advantage in dynamically assessing
ow velocities.
• To mitigate venous compromise, we utilise couplers
as they are efcient and effective for anastomosis
patency and assess blood ow and colour on taking
clamps off (dark blood initially after ischaemia
time). Turgor within other available ap veins is a
sign another venous anastomosis might be of benet
and is considered along with ap colour, capillary
rell time and dermal bleeding assessment.
• Effective ap observations by experienced clinical
staff and prompt re-exploration in theatre if any
signs of compromise are recognised measures to
increase ap salvage rates. Pedicle positioning to
avoid any tension, twisting/kinking or external
compression is crucial (this may require vein
grafts). Sometimes the ap may require inset adjustments necessitating skin grafts to less crucial wound
areas. We recognise the lack of evidence base, but
when anticoagulation is not contraindicated, we
favour Heparin IV bolus on releasing microvascular
clamps and a low dose post-operative infusion for
3days in the revision setting.
49.7 Selected Readings
• Duymaz A, Karabekmez FE, Vrtiska TJ, Mardini S,
Moran SL. Free tissue transfer for lower extremity
reconstruction: a study of the role of computed angiography in the planning of free tissue transfer in the posttraumatic setting. Plast Reconstr Surg. 2009;124(2):
523–9.
In 76 lower extremity trauma patients who underwent
preoperative CTA for free ap reconstruction of the lower
limb the incidence of traumatic occlusion was recorded.
The authors concluded that the incidence of single-vessel
traumatic arterial occlusion within traumatized lower
limbs undergoing free tissue transfer may be as high as
29%. Computed tomographic angiography provided
excellent visualization of lower extremity vasculature,
and its routine use for trauma patients is safe. Flap failure
rates were low when using this technique for preoperative
planning. Flap failure occurred only in patients with evidence of arterial injury. Evidence of arterial occlusion on
computed tomographic angiography may be a risk factor
for limb loss.
• Eccles S, Handley B, Khan U, Nanchahal J, Nayagam S,
McFadyen I, editors. Standards for the management of
open fractures. Published: August 2020.
Standards for the Management of Open Fractures provides an evidence-based approach for the management
of open fractures, focusing on lower limb injuries. It
builds on and expands the National Institute for Health
and Care Excellence (NICE) Guidelines to provide a
practical approach with supporting evidence. The new
edition has been extensively updated and expanded to
include key aspects of management, ranging from setting
up an orthoplastic service, through to dealing with bone
and soft tissue injuries, including in young and older
people, patient rehabilitation and psychological care,
blast injuries, as well as complications such as
infection.

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Y. Sheena et al.
• Chen HC, Chuang CC, Chen S, Hsu WM, Wei
FC.Selection of recipient vessels for free aps to the distal leg and foot following trauma. Microsurgery.
1994;15(5):358–63.
This classic paper by the Chen and colleagues examined
126 patients with Gustillo Type III open fractures that
required free tissue transfer. They found the anterior tibial
artery had a much higher incidence of injury compared to
the posterior tibial artery. This should be borne in mind
when the anterior tibial artery is selected as the recipient
artery in order to prevent reexploration and failure of the
aps. However, the posterior tibial artery is much less
vulnerable to damage in most injuries and is more reliable as the recipient artery.
• Godina M.Preferential use of the posterior approach to
blood vessels of the lower leg in microvascular surgery.
Plast Reconstr Surg. 1991;88:287–91.
This classic paper by Marco Godina describes a mild
muscle splitting approach that provides a wide exposure
to the posterior tibial artery. End to side anastomosis can
be performed in the lateral decubitus position.
References
2. Abou-Foul AK, Borumandi F. Anatomical variants of lower limb
vasculature and implications for free bula ap: systematic review
and critical analysis. Microsurgery. 2016;36(2):165–72.
3. Cho MJ, Kwon JG, Pak CJ, Suh HP, Hong JP.The role of duplex
ultrasound in microsurgical reconstruction: review and technical
considerations. J Reconstr Microsurg. 2020;36(7):514–21.
4. Oni G, Chow W, Ramakrishnan V, Grifths M.Plastic surgeon led
ultrasound. Plast Reconstr Surg. 2018;141(2):300e–9e.
5. Lee GK, Fox PM, Riboh J, Hsu C, Saber S, Rubin GD, Chang
J. Computed tomography angiography in microsurgery: indications, clinical utility, and pitfalls. Eplasty. 2013;13:e42.
6. Gakhal MS, Sartip KA.CT angiography signs of lower extremity
vascular trauma. AJR Am J Roentgenol. 2009;193:W49–57.
7. https://oxfordmedicine.com/view/10.1093/
med/9780198849360.001.0001/med- 9780198849360.
8. Chen HC, Chuang CC, Chen S, Hsu WM, Wei FC.Selection of
recipient vessels for free aps to the distal leg and foot following
trauma. Microsurgery. 1994;15(5):358–63.
9. Godina M, Arnez ZM, Lister GD.Preferential use of the posterior
approach to blood vessels of the lower leg in microvascular surgery.
Plast Reconstr Surg. 1991;88(2):287–91.
10. Yazar S, Lin CH.Selection of recipient vessel in traumatic lower
extremity. J Reconstr Microsurg. 2012;28(3):199–204.
11. Broer PN, Moellhoff N, Mayer JM, Heidekruger PI, Ninkovic M,
Ehrl D. Comparison of outcomes of end-to-end versus end-toside anastomoses in lower extremity free ap reconstructions. J
Reconstr Microsurg. 2020;36(6):432–7.
12. Fujiki M, Miyamoto S, Sakuraba M. Flow-through anastomosis
for both the artery and vein in leg free ap transfer. Microsurgery.
2015;35:536–40.
1. Rosson GD, Singh NK. Devascularising complications of free
bula harvest: peronea arteria magna. J Reconstr Microsurg.
2005;21(8):533–8.

Lymphatic Supermicrosurgery
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TakumiYamamoto andNanaYamamoto
50
50.1 Indications ofLymphatic
Supermicrosurgery
Lymphatic supermicrosurgery includes supermicrosurgical dissection and anastomosis of the collecting lymph
vessels. Since the collecting lymph vessels are usually
smaller than 0.5mm in diameter, supermicrosurgical techniques are required to anastomose them. There are two
indications of lymphatic supermicrosurgery; obstructive
disease (lymphedema) and diseases of leakage (lymphorrhea and lymphocyst).
Lymphedema is a progressive edematous disease
caused by lymph ow obstruction. Lymphatic bypass is
effective to improve lymph ows. As lymph originally
ows into venous circulation at the venous angle, lymphto-venous shunt addresses pathophysiology of obstructive
lymphedema. Supermicrosurgical lymphaticovenular
anastomosis (LVA), in which a lymph vessel is anastomosed to a nearby venule or a small vein in an intima-tointima coaptation, diverts congested lymph ows into
venous circulation.
Lymphorrhea and lymphocyst occur after trauma or surgery to lymph-rich regions, i.e., lymphadenectomy. Surgical
treatment is considered when refractory to conservative therapy. Precise identication of the ruptured lymph vessels is a
key to successful management. The lymph vessels should be
reconstructed, if possible, with supermicrosurgical lymphati-
colymphatic anastomosis (LLA) or LVA.If there is no suitable recipient vessel, the lymph vessel is supermicrosurgically
ligated, but secondary lymphedema may occur because the
major lymph ow is obstructed by the ligation.
T. Yamamoto (*) · N. Yamamoto
Department of Plastic and Reconstructive Surgery, National Center
for Global Health and Medicine, Tokyo, Japan
e-mail: tyamamoto-tky@umin.ac.jp
50.2 Anatomy andPreoperative Imaging
ofLymphatic System
Major lymph pathways run along the major subcutaneous
veins such as the saphenous vein, the cephalic vein, and the
basilic vein. However, precise anatomy is slightly different
from venous anatomy, and lymphatic imaging studies play a
crucial role in lymphatic supermicrosurgery. The gold standard of lymph ow imaging is lymphoscintigraphy, but its
images are too obscure as preoperative mapping for lymphatic supermicrosurgery. MR lymphography and SPECT/
CT allow three-dimensional localization of lymphatics, but it
is not easy to accurately localize the found lymphatics onto
the skin surface for incision site design. Currently, indocyanine green (ICG) lymphography is the most useful imaging
method for diagnosis and preoperative evaluation.
ICG lymphography is performed as follows; 0.1–0.2mL
of 0.25% ICG is intradermally injected at the distal limb
(usually at second web space of the hand/foot, and several
points), and uorescent images are obtained using a nearinfrared camera system. Dynamic ICG lymphography,
dual-phase observation ICG lymphography, is important for
thorough examination of lymphatic system; observed immediately after ICG injection (early transient phase), and 2–72h
after injection (late plateau phase) [Fig. 50.1]. Typical ICG
lymphography ndings include normal linear pattern and
abnormal dermal backow (DB) patterns (Splash, Stardust,
and Diffuse pattern) [Fig. 50.2]. At an early phase, Linear
pattern is marked to localize lymph vessels. At a late phase,
extension of DB pattern is marked for severity evaluation of
lymphedema.
ICG lymphography stage is useful for pathophysiological severity staging of secondary lymphedema and to consider indication of LVA [Table 50.1]. LVA is best indicated
for ICG stage II-IV.Prophylactic LVA may be considered for
ICG stage I, and lymph node transfer is better indicated for
ICG stage V.
© Springer Nature Switzerland AG 2023
D. Nikkhah et al. (eds.), Core Techniques in Flap Reconstructive Microsurgery, https://doi.org/10.1007/978-3-031-07678-7_50
489

490
Fig. 50.1 Dynamic ICG lymphography; dual-phase
observation at an early transient phase, and at a late plateau
phase
Fig. 50.2 Characteristic ICG
lymphography ndings
T. Yamamoto and N. Yamamoto
Table 50.1 ICG lymphography stage
ICG stage Lymphographic ndings
Stage 0 Linear pattern only (no DB pattern)
Stage I Linear pattern+splash pattern
Stage II Linear pattern+DB pattern (1 region)
Stage III Linear pattern+DB pattern (2 regions)
Stage IV Linear pattern+DB pattern (3 regions)
Stage V DB pattern only (no linear pattern)
ICG indocyanine green, DB dermal backow
a
Splash pattern is usually seen around the axilla/groin
b
Upper/lower extremity is divided into three regions; the upper-arm/
thigh, the forearm/lower-leg, and the hand/foot. Stardust pattern is usually seen in DB pattern; diffuse pattern may be seen
a
b
b
b
50.3 Recommended Surgical Sites
forLymphedema andLympho-rrhea/cyst (A Figure withSurface Markings)
In LVA for lymphedema treatment, slightly sclerotic lymph
vessels are recommended for anastomosis. With lymphedema progression, lymph vessels become sclerotic with less
Table 50.2 Lymphosclerosis severity classication
Lymph vessel characteristics
Severity
s0 Translucent Expandable Identiable Very thin
s1 White Expandable Identiable Thin
s2 White Not expandable Identiable Thick
s3 White Not expandable Not
Appearance Expandability Lumen
identiable
Wall
thickness
Very thick
lymph ows inside; lymphosclerosis. Lymphosclerosis
grades are divided into “s0,” “s1,” “s2,” and “s3” [Table
50.2]. A slightly sclerotic “s1” lymph vessel usually has high
lymph ow, and is best indicated for LVA. “s1” lymph vessels can be most frequently found in the “overlapping
region.” The “overlapping region” is identied by dynamic
ICG lymphography, where Linear pattern is seen at an early
phase and DB pattern at a late phase [Fig. 50.3]. Incision
sites for LVA should be designed in the overlapping regions
revealed by dynamic ICG lymphography.

50 Lymphatic Supermicrosurgery
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Fig. 50.3 Skin incision sites
in LVA surgery for
lymphedema. Overlapping
region revealed by dynamic
ICG lymphography
491
Fig. 50.4 “Direct approach” skin incision site (red line) for
lympho-rrhea/-cyst
For lympho-rrhea/-cyst treatment, skin incision should
be designed on the lesion, if possible [50.4]. Direct
approach allows secure identification and reconstruction
of causative ruptured lymph vessels, and capsulectomy
for lymphocyst if needed. When the direct approach is
difficult, for example, as wound problem risk is considered high due to radiation or exposed artificial materials,
indirect approach is applied; skin incision should be
designed distally to the lesion as close as possible,
according to preoperative ICG lymphography findings
[Fig. 50.5].
Fig. 50.5 “Indirect approach” skin incision site (red line) for
lympho-rrhea/-cyst
50.4 Supermicrosurgical LVA for
Lymphedema: AStep-by-Step Guide
• Step 1. Careful Skin Incision [Fig. 50.6]. After local
inltration anesthesia with 1% lidocaine with 1:100,000
epinephrine, skin incision is made on a designed line.
Attention is paid not to injure the subdermal veins. Whole
procedures, from skin incision to skin closure, should be
performed under an operating microscope.
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