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482
Y. Sheena et al.
marks guide the approach and thigh vasculature is less prone to variation, but it is worth being aware of differences (even within the same individual on the contralateral side) in the origin, calibre and course of leg vessels. One important vari­ant being the Peronea Arteria Magna, where one or both of the Anterior and Posterior Tibial arteries are congenitally small or absent, making the Fibular artery the dominant blood supply to the distal leg in 5–9% of patients [1, 2]. It is wise to note the risks of atherosclerotic occlusive or deep venous thrombotic disease, and some would argue colour Doppler ultrasound performed by radiologically trained sur­geons gives the most useful dynamic information on the con-
dition of the vessels for microsurgery [3, 4]. In elective oncological or chronic infection cases, vessels involvement is worth investigating and in trauma—fractures, penetrating injuries and external forces may have caused vessel damage precluding their use or mandating vascular reconstruction during microsurgery. It is our common practice to utilise thermal imaging and hand-held Doppler for skin perforator mapping/ap design, and all our trauma patients have routine pre- operative CT angiography, which many use as the ‘gold standard’ investigation [5, 6] to dene the pre-operative vas­cular state, relationship to orthopaedic injuries and to plan microsurgical reconstruction (see Fig.49.1).
Fig. 49.1 CT angiograms on the left showing normal bilateral 3-vessel run off; and on the right in an open left tibia and bula fracture with arterial injury signied by no ow in the proximal 10cm of the anterior tibial and peroneal arteries
49 Lower Limb Recipient Vessels Access
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49.4 Recipient Vessel Access [See Fig.49.2
forLeg Surface Markings]
If appropriate recipient vessels are not available within the defect to be reconstructed, the principle of access is to con­nect the defect to the safe fasciotomy lines [7] via the short­est necessary incisions (see Fig. 49.2). The incisions go through skin, subcutaneous fat, fascia and into the relevant plane retracting tendons/muscles/nerves to isolate the selected recipient vessels with the nal preparation per­formed under the microscope. The most commonly used recipient vessels are the anterior or posterior tibials. There are pros/cons of each and we are aware of past schools of thought preferring the PTA [8], with Godina describing a mid-axial approach splitting the medial and lateral gastroc­nemius muscle [9]. More recent experience shows the ATA to be just as reliable, even when the vessels are damaged in the zone of trauma, they have equivalent ap success rates when dissected proximal to injury [10]. Other not infre­quently utilised recipient vessels include the dorsalis pedis, supercial femoral and its descending genicular branches. Less commonly utilised ‘get out of jail’ vessels include the bular, popliteal and vein grafts or AV loops to more proxi­mal thigh vessels. The PTA is commonly dominant in sup­plying blood distally, so we rarely perform end-to-end anastomosis on this artery. It has predictable perforators to surrounding muscles and medial leg skin (described at 5, 10 and 15cm above the medial malleolus) and these are ideal recipients for free ap microsurgery. We prefer to perform end-to-side arterial anastomoses to maintain axial limb blood ow, especially when less than three healthy vessels perfuse the distal leg or in patients with peripheral vascular disease [11]. In a ‘one vessel’ leg our preference is to use a ‘ow­through’ free ap to reconstruct the damaged vessels, and some surgeons believe these may be associated with improved ap survival [12]. Some cases with gures to illus­trate vessel access follow.
483
1. Dorsalis Pedis Vessels [Figs. 49.3 and 49.4]: Useful for dorsal foot wounds when the vessels are
available. DPA is the continuation of the ATA distal to the ankle joint and terminates as the First Dorsal Metatarsal Artery and the Deep Plantar Artery. Its course can be sur-
Fig. 49.2 Image of left leg with subcutaneous Tibial borders (Black solid lines), fasciotomy election lines (Green dashed) and PTA perfora­tors medially (Red crosses); and Right leg illustrating three potential open fracture soft tissue defects (Red hatched) with access incisions (Blue curved lines) in relation to fasciotomy lines. The inferior defect to ATA, middle 1/3 defect to PTA and proximal defect to DGA or SFA
face landmarked by the line joining the mid-point of the malleoli and the proximal rst metatarsal interval. Find the pulse just lateral to Extensor Hallucis Longus (EHL) and medial to Extensor Digitorum Longus (EDL) most readily palpable at the distal navicular bony prominence. During dissection, identify and protect the adjacent deep peroneal nerve.
2. Anterior Tibial Vessels [Figs. 49.5, 49.6, 49.7, 49.8, 49.9,
and 49.10]:
The surface landmark line of the ATA is described by a line joining the medial bula head to the dorsal mid­malleolar point. Find these vessels distally between the Tibialis Anterior (TA) and EHL tendons. More proxi­mally these vessels become deeper between the muscles of TA medially and EDL laterally. Protect the adjacent deep peroneal nerve during vessel dissection. The images show a lateral ankle open fracture (xed by diastasis
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Fig. 49.3 Left dorsal foot defect with skin surface markings for access to the dorsalis pedis vessels
Fig. 49.4 DPA with its two VCs dissected and clear for microsurgery at site with blue background
Fig. 49.6 Right ATA prepared for microsurgery with its two anking VCs between tibialis anterior (retracted medially/above) and the EHL/ EDL muscles (retracted laterally/below, along with the deep peroneal nerve seen distally on right side of wound in image)
Fig. 49.7 Two-perforator ALT ap connected end-to-end on to the right ATA seen from lateral side
Fig. 49.5 Right lateral ankle defect with surface markings for access to anterior tibial vessels
Fig. 49.8 ALT ap anastomoses showing two venous couplers end-to­end on to ATA VCs
49 Lower Limb Recipient Vessels Access
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Fig. 49.9 ALT ap inset to cover the defect distally and the vessel access incision/wound proximally
485
Fig. 49.11 Right distal tibial open fracture with IM nail in situ show­ing access incision to PTA
Fig. 49.10 ALT ap dressings at end of procedure demonstrating monitoring window and pressure care instructions around anastomosis site
screw) with a soft tissue defect in a leg with three-vessel run-off with an access incision towards the distal anterior tibial vessels allowing an end-to-end arterial hand-sewn and two venous coupled ALT (double perforator) ap anastomoses.
3. Posterior Tibial Vessels [Figs. 49.11, 49.12, and 49.13]: The PTA runs from the end of the PA (2.5 cm below
the mid-Popliteal fossa) to a point between the medial malleolus and the heel where the pulse should be easily palpable. Passing under the ankle exor retinaculum the mneumonic ‘Tom, Dick and Nervous Harry’ serves to remind of the anterior to posterior relationships of Tibialis Posterior, Flexor Digitorum Longus, the Tibial Artery, Tibial Nerve and Flexor Hallucis Longus. In the leg, the
Fig. 49.12 MSAP ap over tibial defect with end-to-side anastomosis to PTA and one ap vein coupled to its VC and the second to a super­cial system vein
Fig. 49.13 MSAP ap inset resurfacing defect. Note proximal half of ap utilised to cover vessels
PTA travels with its Venae and the Tibial nerve in the deep posterior compartment (deep to the transverse intermuscular septum supercial to the Tibialis Posterior muscle) between the FDL and FHL muscles. It is usually
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approached medially, taking care not to injure the Great Saphenous Vein (GSV) and Saphenous Nerve, by releas­ing and retracting the crural fascia and Soleus off the posterior-medial Tibial border. Care must be taken not to injure the Tibial nerve. This open Tibial fracture had IM nail xation and immediate contralateral MSAP free ap reconstruction. The incision allowed access to the PTA, its VCs and a supercial vein were also identied, dis­sected and utilised. An end-to-side arterial and two cou­pled venous anastomoses (one to deep VC and one to the supercial recipient). Note that swelling in the acute trauma setting often requires larger aps be harvested in anticipation of failure to close the vessel access incision and to cover the pedicle.
4. Supercial Femoral and Descending Genicular Vessels
[49.14].
These vessels are useful in cases of higher leg, knee or thigh defects that require reconstruction. The SFA is sur­face marked by the upper two-thirds of a line from the mid- inguinal point (midpoint between Pubic Symphasis Pubis and Anterior Superior Iliac Spine) and the Adductor Tubercle. As aforementioned, it travels under Sartorius and can be accessed between Adductor Longus (easily pal­pable with the relaxed thigh abducted and externally rotated). The SFA usually gives off a branch medially called the Descending Genicular Artery (DGA) approxi­mately 13cm above the knee joint, which travels between Sartorius and Vastus Medialis in close proximity to the Saphenous nerve. This nerve and the GSV should be iden­tied and protected when preparing these vessels. See Fig.49.14 for an image showing DGA vessel preparation to receive an LD free ap to reconstruct a proximal tibial Gustillo 3B fracture with single vessel distal run off and poor Popliteal branches on CTA.
49.5 Core Surgical Techniques inRecipient Vessel Harvest
To summarise:
1. Recipient vessel selection is based on defect location and
the condition of local vessels on CTA.
2. Surface landmarks and hand-held Doppler conrm access
incision placement. Utilise shortest line from defect to safe fasciotomy lines (see Fig.49.2).
3. Tourniquet control dissection for bloodless eld and
Loupe magnication allow accurate identication of nerves, supercial veins (that may be utilised during pri­mary or rescue anastomoses).
4. Adequate assistance and good retraction allows safe iden-
tication and control of selected vessels.
5. Final preparation under microscope to conrm satisfac-
tory vessel walls, an arterial ‘squirt test’ and low pressure venous ‘ush test’ are key requirements.
6. Aim for perforator-to-perforator (when available) end-to-
end anastomosis or an end-to-side anastomosis with more major recipient vessels. End-to-side anastomosis has comparable patency and ap outcomes, addresses vessel size mismatch and preserves distal limb perfusion and preserves recipient vessel options if subsequent recon­struction or further free ap surgery is ever required.
7. Check for venous backow and low resistance heparin-
ised saline ushing. Anastomosis proximity to valves is not usually an issue. There must be a low threshold in connecting an extra vein to the ap, if possible, especially if turgid second ap vein with rst vein owing, or if high pressure recipients (we mitigate DVT risk by utilising a supercial second vein recipient if available).
8. Assess immediate ow with Acland test, note darker
blood return to vein on clamps down and usual ap obser­vations noting colour of bleeding from dermal edges with low threshold to revision/additional vein anastomoses or Vein Grafts if required.
Fig. 49.14 Medial thigh access incision showing the DGA utilised as recipient vessel for proximal tibial reconstruction
49.6 Pearls andPitfalls
Pearls
• Utilise pre-operative imaging—Angiography or Colour Doppler Ultrasound (CDU).
• Identify/protect any supercial veins around defect for reconstruction. We aim to anastomose two veins if the ap pedicle has two VCs. Utilising a recipient site VC and a supercial vein mitigates the risks of rely­ing on just the deep or supercial venous systems.
49 Lower Limb Recipient Vessels Access
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487
• Assess vein wall condition, blood backow on cut­ting and ease of ushing with heparinised saline.
• Careful ap templating taking extra skin to account for and cover the vessels and access incision.
• Arterial assessment considers non-contused appear­ance, visible/palpable pulsatility (we use intra­operative pencil Doppler) and most importantly the ‘squirt test’ to conrm adequate ow pressure. After microsurgery conrm vessels not twisted, kinked, or under tension/pressure. We sometimes utilise a small piece of fat to help cushion the pedi­cle around the anastomosis site.
Pitfalls
• Regardless of microsurgical planning and execu­tion, the adequacy of excision margins will deter­mine outcome. It is complete oncological clearance or radical wound excision to healthy tissues in the infective or trauma setting that will minimise can­cer recurrence, infection and non-union. It is crucial for the microsurgeon to perform this well or work closely with the ablative surgeon to do so.
• Any factor from the pre- to the post-operative course can compromise the vessels, ap and entire reconstruction. The adequacy of your local pre­operative clinical and radiological assessment helps guide the operative vessel selection and it is well worth learning to correlate your local angiography procedure (for DSA, CTA, MRA, etc.) with opera­tive ndings.
• Adequate arteries are necessary, but not sufcient for ap success. Venous congestion is the greater risk so identifying injuries or deep vein thrombosis preoperatively can help plan to mitigate ap venous compromise. Colour Doppler ultrasound may have an imaging advantage in dynamically assessing ow velocities.
• To mitigate venous compromise, we utilise couplers as they are efcient and effective for anastomosis patency and assess blood ow and colour on taking clamps off (dark blood initially after ischaemia time). Turgor within other available ap veins is a sign another venous anastomosis might be of benet and is considered along with ap colour, capillary rell time and dermal bleeding assessment.
• Effective ap observations by experienced clinical staff and prompt re-exploration in theatre if any signs of compromise are recognised measures to
increase ap salvage rates. Pedicle positioning to avoid any tension, twisting/kinking or external compression is crucial (this may require vein grafts). Sometimes the ap may require inset adjust­ments necessitating skin grafts to less crucial wound areas. We recognise the lack of evidence base, but when anticoagulation is not contraindicated, we favour Heparin IV bolus on releasing microvascular clamps and a low dose post-operative infusion for 3days in the revision setting.
49.7 Selected Readings
• Duymaz A, Karabekmez FE, Vrtiska TJ, Mardini S, Moran SL. Free tissue transfer for lower extremity reconstruction: a study of the role of computed angiog­raphy in the planning of free tissue transfer in the post­traumatic setting. Plast Reconstr Surg. 2009;124(2): 523–9.
In 76 lower extremity trauma patients who underwent preoperative CTA for free ap reconstruction of the lower limb the incidence of traumatic occlusion was recorded. The authors concluded that the incidence of single-vessel traumatic arterial occlusion within traumatized lower limbs undergoing free tissue transfer may be as high as 29%. Computed tomographic angiography provided excellent visualization of lower extremity vasculature, and its routine use for trauma patients is safe. Flap failure rates were low when using this technique for preoperative planning. Flap failure occurred only in patients with evi­dence of arterial injury. Evidence of arterial occlusion on computed tomographic angiography may be a risk factor for limb loss.
• Eccles S, Handley B, Khan U, Nanchahal J, Nayagam S, McFadyen I, editors. Standards for the management of open fractures. Published: August 2020.
Standards for the Management of Open Fractures pro­vides an evidence-based approach for the management of open fractures, focusing on lower limb injuries. It builds on and expands the National Institute for Health and Care Excellence (NICE) Guidelines to provide a practical approach with supporting evidence. The new edition has been extensively updated and expanded to include key aspects of management, ranging from setting up an orthoplastic service, through to dealing with bone and soft tissue injuries, including in young and older people, patient rehabilitation and psychological care, blast injuries, as well as complications such as infection.
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• Chen HC, Chuang CC, Chen S, Hsu WM, Wei FC.Selection of recipient vessels for free aps to the dis­tal leg and foot following trauma. Microsurgery. 1994;15(5):358–63.
This classic paper by the Chen and colleagues examined 126 patients with Gustillo Type III open fractures that required free tissue transfer. They found the anterior tibial artery had a much higher incidence of injury compared to the posterior tibial artery. This should be borne in mind when the anterior tibial artery is selected as the recipient artery in order to prevent reexploration and failure of the aps. However, the posterior tibial artery is much less vulnerable to damage in most injuries and is more reli­able as the recipient artery.
• Godina M.Preferential use of the posterior approach to blood vessels of the lower leg in microvascular surgery. Plast Reconstr Surg. 1991;88:287–91.
This classic paper by Marco Godina describes a mild muscle splitting approach that provides a wide exposure to the posterior tibial artery. End to side anastomosis can be performed in the lateral decubitus position.
References
2. Abou-Foul AK, Borumandi F. Anatomical variants of lower limb vasculature and implications for free bula ap: systematic review and critical analysis. Microsurgery. 2016;36(2):165–72.
3. Cho MJ, Kwon JG, Pak CJ, Suh HP, Hong JP.The role of duplex ultrasound in microsurgical reconstruction: review and technical considerations. J Reconstr Microsurg. 2020;36(7):514–21.
4. Oni G, Chow W, Ramakrishnan V, Grifths M.Plastic surgeon led ultrasound. Plast Reconstr Surg. 2018;141(2):300e–9e.
5. Lee GK, Fox PM, Riboh J, Hsu C, Saber S, Rubin GD, Chang J. Computed tomography angiography in microsurgery: indica­tions, clinical utility, and pitfalls. Eplasty. 2013;13:e42.
6. Gakhal MS, Sartip KA.CT angiography signs of lower extremity vascular trauma. AJR Am J Roentgenol. 2009;193:W49–57.
7. https://oxfordmedicine.com/view/10.1093/
med/9780198849360.001.0001/med- 9780198849360.
8. Chen HC, Chuang CC, Chen S, Hsu WM, Wei FC.Selection of recipient vessels for free aps to the distal leg and foot following trauma. Microsurgery. 1994;15(5):358–63.
9. Godina M, Arnez ZM, Lister GD.Preferential use of the posterior approach to blood vessels of the lower leg in microvascular surgery. Plast Reconstr Surg. 1991;88(2):287–91.
10. Yazar S, Lin CH.Selection of recipient vessel in traumatic lower extremity. J Reconstr Microsurg. 2012;28(3):199–204.
11. Broer PN, Moellhoff N, Mayer JM, Heidekruger PI, Ninkovic M, Ehrl D. Comparison of outcomes of end-to-end versus end-to­side anastomoses in lower extremity free ap reconstructions. J Reconstr Microsurg. 2020;36(6):432–7.
12. Fujiki M, Miyamoto S, Sakuraba M. Flow-through anastomosis for both the artery and vein in leg free ap transfer. Microsurgery. 2015;35:536–40.
1. Rosson GD, Singh NK. Devascularising complications of free bula harvest: peronea arteria magna. J Reconstr Microsurg. 2005;21(8):533–8.
Lymphatic Supermicrosurgery
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TakumiYamamoto andNanaYamamoto
50
50.1 Indications ofLymphatic Supermicrosurgery
Lymphatic supermicrosurgery includes supermicrosurgi­cal dissection and anastomosis of the collecting lymph vessels. Since the collecting lymph vessels are usually smaller than 0.5mm in diameter, supermicrosurgical tech­niques are required to anastomose them. There are two indications of lymphatic supermicrosurgery; obstructive disease (lymphedema) and diseases of leakage (lymphor­rhea and lymphocyst).
Lymphedema is a progressive edematous disease caused by lymph ow obstruction. Lymphatic bypass is effective to improve lymph ows. As lymph originally ows into venous circulation at the venous angle, lymph­to-venous shunt addresses pathophysiology of obstructive lymphedema. Supermicrosurgical lymphaticovenular anastomosis (LVA), in which a lymph vessel is anasto­mosed to a nearby venule or a small vein in an intima-to­intima coaptation, diverts congested lymph ows into venous circulation.
Lymphorrhea and lymphocyst occur after trauma or sur­gery to lymph-rich regions, i.e., lymphadenectomy. Surgical treatment is considered when refractory to conservative ther­apy. Precise identication of the ruptured lymph vessels is a key to successful management. The lymph vessels should be reconstructed, if possible, with supermicrosurgical lymphati- colymphatic anastomosis (LLA) or LVA.If there is no suit­able recipient vessel, the lymph vessel is supermicrosurgically ligated, but secondary lymphedema may occur because the major lymph ow is obstructed by the ligation.
T. Yamamoto (*) · N. Yamamoto Department of Plastic and Reconstructive Surgery, National Center for Global Health and Medicine, Tokyo, Japan e-mail: tyamamoto-tky@umin.ac.jp
50.2 Anatomy andPreoperative Imaging ofLymphatic System
Major lymph pathways run along the major subcutaneous veins such as the saphenous vein, the cephalic vein, and the basilic vein. However, precise anatomy is slightly different from venous anatomy, and lymphatic imaging studies play a crucial role in lymphatic supermicrosurgery. The gold stan­dard of lymph ow imaging is lymphoscintigraphy, but its images are too obscure as preoperative mapping for lym­phatic supermicrosurgery. MR lymphography and SPECT/ CT allow three-dimensional localization of lymphatics, but it is not easy to accurately localize the found lymphatics onto the skin surface for incision site design. Currently, indocya­nine green (ICG) lymphography is the most useful imaging method for diagnosis and preoperative evaluation.
ICG lymphography is performed as follows; 0.1–0.2mL of 0.25% ICG is intradermally injected at the distal limb (usually at second web space of the hand/foot, and several points), and uorescent images are obtained using a near­infrared camera system. Dynamic ICG lymphography, dual-phase observation ICG lymphography, is important for thorough examination of lymphatic system; observed imme­diately after ICG injection (early transient phase), and 2–72h after injection (late plateau phase) [Fig. 50.1]. Typical ICG lymphography ndings include normal linear pattern and abnormal dermal backow (DB) patterns (Splash, Stardust, and Diffuse pattern) [Fig. 50.2]. At an early phase, Linear pattern is marked to localize lymph vessels. At a late phase, extension of DB pattern is marked for severity evaluation of lymphedema.
ICG lymphography stage is useful for pathophysiologi­cal severity staging of secondary lymphedema and to con­sider indication of LVA [Table 50.1]. LVA is best indicated for ICG stage II-IV.Prophylactic LVA may be considered for ICG stage I, and lymph node transfer is better indicated for ICG stage V.
© Springer Nature Switzerland AG 2023 D. Nikkhah et al. (eds.), Core Techniques in Flap Reconstructive Microsurgery, https://doi.org/10.1007/978-3-031-07678-7_50
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Fig. 50.1 Dynamic ICG lymphography; dual-phase observation at an early transient phase, and at a late plateau phase
Fig. 50.2 Characteristic ICG lymphography ndings
T. Yamamoto and N. Yamamoto
Table 50.1 ICG lymphography stage
ICG stage Lymphographic ndings Stage 0 Linear pattern only (no DB pattern) Stage I Linear pattern+splash pattern Stage II Linear pattern+DB pattern (1 region) Stage III Linear pattern+DB pattern (2 regions) Stage IV Linear pattern+DB pattern (3 regions) Stage V DB pattern only (no linear pattern)
ICG indocyanine green, DB dermal backow
a
Splash pattern is usually seen around the axilla/groin
b
Upper/lower extremity is divided into three regions; the upper-arm/ thigh, the forearm/lower-leg, and the hand/foot. Stardust pattern is usu­ally seen in DB pattern; diffuse pattern may be seen
a
b
b
b
50.3 Recommended Surgical Sites forLymphedema andLympho-rrhea/­cyst (A Figure withSurface Markings)
In LVA for lymphedema treatment, slightly sclerotic lymph vessels are recommended for anastomosis. With lymph­edema progression, lymph vessels become sclerotic with less
Table 50.2 Lymphosclerosis severity classication
Lymph vessel characteristics
Severity s0 Translucent Expandable Identiable Very thin s1 White Expandable Identiable Thin s2 White Not expandable Identiable Thick s3 White Not expandable Not
Appearance Expandability Lumen
identiable
Wall thickness
Very thick
lymph ows inside; lymphosclerosis. Lymphosclerosis grades are divided into “s0,” “s1,” “s2,” and “s3” [Table
50.2]. A slightly sclerotic “s1” lymph vessel usually has high
lymph ow, and is best indicated for LVA. “s1” lymph ves­sels can be most frequently found in the “overlapping region.” The “overlapping region” is identied by dynamic ICG lymphography, where Linear pattern is seen at an early phase and DB pattern at a late phase [Fig. 50.3]. Incision sites for LVA should be designed in the overlapping regions revealed by dynamic ICG lymphography.
50 Lymphatic Supermicrosurgery
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Fig. 50.3 Skin incision sites in LVA surgery for lymphedema. Overlapping region revealed by dynamic ICG lymphography
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Fig. 50.4 “Direct approach” skin incision site (red line) for lympho-rrhea/-cyst
For lympho-rrhea/-cyst treatment, skin incision should be designed on the lesion, if possible [50.4]. Direct approach allows secure identification and reconstruction of causative ruptured lymph vessels, and capsulectomy for lymphocyst if needed. When the direct approach is difficult, for example, as wound problem risk is consid­ered high due to radiation or exposed artificial materials, indirect approach is applied; skin incision should be designed distally to the lesion as close as possible, according to preoperative ICG lymphography findings [Fig. 50.5].
Fig. 50.5 “Indirect approach” skin incision site (red line) for lympho-rrhea/-cyst
50.4 Supermicrosurgical LVA for Lymphedema: AStep-by-Step Guide
Step 1. Careful Skin Incision [Fig. 50.6]. After local
inltration anesthesia with 1% lidocaine with 1:100,000 epinephrine, skin incision is made on a designed line. Attention is paid not to injure the subdermal veins. Whole procedures, from skin incision to skin closure, should be performed under an operating microscope.