підручники. біохімія / Uchebnik_po_Biokhimii_E_S_Severin
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NH2 |
0 |
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0 |
0 |
0 |
Ni:N |
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II |
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II |
II |
f"' |
I I |
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-o-P-O-P-O-P-oiH{ |
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I |
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I |
I |
H |
H N |
~ |
o- |
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o- |
0- |
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N |
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H |
H |
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',,// |
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Mg2+ |
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OH |
OH |
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CxeMa
CxeMaTWIHO po11h K0<paKTopa rrp:u B3a:uMo)l,e:U- CTB:U:U <t>epMeHTa :u Cy6cT])aTa MO)f(IfO npe)l,CTaBHTh KaK KOMIIJieKc E-S-Me, r)l,e E - <t>epMeHT, S - cy6CT])aT, Me - :uoH MeTaJTJia.
B KaqecTBe rrp:uMepa MO)KHO rrp:ueecT:u pacrro11o:x<:eH:ue cy6CT])aTOB B aKTHBHOM ueHT])e reKCOKHHa3hl (p:UC. 2-3).
f eKCOKHHa3a KaTaJI:U3:upyeT rrepeHOC KOHUeBOro, y-<t>oc<t>arnoro ocTaTKa MOJieKYJihI AT<l> Ha rmoK03Y c o6paJoBaH:ueM fJIIOK03o-6-<t>oc<t>arn:
CH2-0H |
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CH2-0-P03H2 |
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H~oH |
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reKcoK111Haaa H~H |
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OH |
H |
+ AT¢ |
~H |
H |
+ A,Q4> |
HO |
OH |
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HO |
OH |
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H |
OH |
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H |
OH |
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r moKoaa |
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rmoKoao-6-cj:JocQJaT |
rmoK03a
r111,QparnpoBaHHblM IAOH MarHIAfl
PMc. 2-3. Yt.t&CTMe MOHOB MarHMtl B npMcoeAMHeHMM cy6cTpaT8 a aKTMBHOM ~HTpe reKCOKMHB3bl. B aKT1tBHOM 1..1eHTpe reKcoK11Haab1 ecTb y'iaCTKltCBfl3blBaHIAfl AJlfl MOneKYnbl rmOK03bl IA KOMnneKca Mg2•-AT<l>. B peaynbTaTe cjlepMeHTaTIABHO~ peaKL.11111 npo111cXOJ:11AT nepeHOC KOHL1eeoro, y-cjloccflaTHOro OCTaTKa MoneKYnbl AT<l> Ha rn10K03y c 06paaoeaH1AeM rnlOK000-6-<f>occpara.
11oH Mg2+ yqacTByeT B rrp:ucoe)l,:uHeH:u:u :u «rrpa- B:uJihHOM» op:ueHrnu:u:u MOJieKYJihI AT<l> B aKT:uBHOM ueHT])e <t>epMema, oc11a6mrn: <t>oc<t>o3<t>:upHy10 CBH3h H 0611erqaH rrepeHOC <t>oc<t>arn Ha fJIIOK03y.
HoHbl MeTaJIJia - cTa6HJIH3aropw aKTHBnoro u;eHTpa «l>epMema
B HeKOTOphIX c11yqaHx :uoHhl MeTaJIJia CJIY:x<:aT «MOCT:UKOM» Me)l()J;y <t>epMeHTOM H cy6cTpaTOM. OH:u BhIIIOJIHHIOT <l>YHKU:UIO cTa6HJI:u3aTOpoe aK- T:UBHoro ueHT])a, 0611erqaH rrp:ucoe)l,:uHeH:ue K HeMY cy6cTpaTa :u rrpOTeKaHHe X:UM:uqecKOM peaKQHH. B pH)l,e c11yqaee HOH MeTaJIJia Mo:x<:eT crroco6cTBoBaTh rrp:ucoe)l,:uHeHmo Ko<t>epMeHTa. I1epeq:uc11eHHh1e Bhlllle <l>YHKU:U:U BhIIIOJIHHIOT TaK:Ue MeTaJIJlhl, KaK Mg2+, Mn2+, Zn2+, Co2+, Mo2+. B OTCYTCTB:Ue MeTaJIJia 3T:U <t>epMeHThl aKTHBHOCThIO He 0611a)J;a10T. TaK:ue <t>epMeHThI rroJiyqrum HaJBaHHe «MeTaJIJI03H3HMhI». CxeMaTwrno )l,aHHhIM rrpou,ecc B3aHMO)l,ettCTBHH <t>epMeHTa, cy6cTpaTa :u MeTaJIJia MO:x<:HO rrpe)l,CTaBHTh CJie)l,YIQIU:UM o6pa30M:
E-Me-S
K MeTaJIJI03H3HMaM OTHOCHT, Harrp:uMep, <t>epMeHT rr:upyeaT KHHa3y (p:uc. 2-4), KaTaJIH3:UpyIOIUHH peaK.UHIO:
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0 |
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II |
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II |
c-o- |
+NICI> |
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c-o- +AT¢ |
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1 |
Pol· |
1 |
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C-0 - |
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C=O |
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II |
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I |
CH2 |
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CH3 |
Cl>occj:JoeHonn111pyeaT |
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n111pyeaT |
2. POAb Mem411.1106 B cma6UAwaquu mpemu11noii u 11emsepmu11noii cmpyKmypbl tjJepMenma
110HhI MeTaJIJIOB o6ecrreqHBaIOT coxpaHett:ue BTOplfqttott, TpeTH'IHOH,qeTBepTWIHOM CT])YKTYPhI MOJieKYJihl <t>epMeHTa. TaKHe <t>epMeHThI B OTCyTCTBHe
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<l>oc¢oeH011m1pyeaT
i.10H MarHll!s:I
YYacToK ces:1Jb1eaH111s:1
AA<I>
PMC. 2-4. Y'l&CTMeMOHOB M&fHMll B npMCoe,qMHeHMM cy6cTpaTa B &KTMBHOM 4eHTpe nMpyeaTKMH83bl. AKTMBHblM 1..19HTP n11pyBaTKMHa3bl MMeeT y4aCTKM CBff3blBaHMff Aflff cjloc:QJoeHonn11pysaTa"" AJ:l<l>. Mg2•y4acTByeT B CTa611n113a41111 aKTMBHOro 1..19HTpa, 4TO o6ner4aeT np11coei:111HeH11e cjloccjloeHonn11pysaTa. B xoi:1e cpepMeHTaTMBHoi1 peaK41111 06pa3yeTcff n11pysaT"" AT<l>.
MOHOB Me'TarrJIOB crroco6Hhl K XHMWieCKOMY KaTa-
Jil13Y, o.ri:ttaKo OHM ttecTa6MJihHhI. Hx mcrttBHOCTh
CHIDKaeTCH 11 AIDKe rroJIHOCThIO ncqe3aer rrptt tte6oJih-
nrnx H3MeHeHHHX pH, TeMrrepazypbI 11 .ri:pyrMX He-
3Ha'IlITeJihHhIXtt3MeHeHHHX BHelllHero OKP)')K:eHHH.
TaKMM o6pa3oM, ttOHhI MerarrJIOB BhIIIOJIIDIIOT cPYH-
KI.IJ110 CTa6ttJIH3aTOpOB OIITMMaJibHOM KOHcPOPMamm
6eJIKOBOM MOJieKYJihI.
Httor.ri:a B cTa6MJIH3aUtttt BToptt'IHOtt11 TpeTnq-
HOtt CTPYKTYPhI rrpMHHMaIOT yqacTtte HOHhl me-
Jl0lJ:H0-3eMeJihHhIX MeTaJIJIOB. TaK, AJIH rroA.!l;ep)l(a-
HHH TpeTH'IHOM KOHcPOPMaUHH rrttpyBaTKHHa3bI Heo6xo.ri:ttMhI ttOHhI K +.
/:(AA cTa6ttJitt3aUHM lJ:eTBepT11qttoi1 CTPYKTYPhI
aJIKOfOJlb)J;erH)J;poreHa3hI, KaTaJIH3HPyIOIUett peaK-
UHIO OKHCJieHHH 3TaHOJia, He06XO)J;HMhI HOHhl UMH-
Ka. MKOfOJlhAem.ri:poreHa3a COCTOHT 113 4 cy6'heAHHHU c MOJieKYAAPHOH MaCCOH 151 I<):(. B COCTaB cllepMeHTa BXO.!l;HT 4 aTOMa Zn2+. Y.ri:aJieHHe Zn2+
rrpttBOAHT K IlOTepe aKTHBHOCTH cllepMeHTa 3a C'IeT AHCCOUHaUMH Ha 4 HeaKTHBHhie cy6ne)J;HHMUhI c MOJieKYJIHPHOM MaCCOH 36 I<):( (pttc. 2-5).
3. POAb Mema.tl.!lOB 6 rjJepMenmamu6HOM
Kamt1J1U3e
He Mettee BIDKHYJO poJlh OTBOAHT ttoHaM MeraJI-
JIOB B ocyiuecTBJieHHH cllepMeHTaTHBHOfO KaTaJIJ13a.
Y'lacTHee 3JieK'fPO!J>HJibHOMKaTaJIH3e
Ha1160Jiee qacTo 3TY Q:>yHKUHIO BhIITOJIHHIOT
HOHhI MeTaJIJIOB c rrepeMeHHOM BaJieHTHOCThIO,
HMe10m11e CB060AHYIO d-op6ttTaJib H BhICzynaIO-
36 K,Q |
36 K,Q |
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Zn2+ |
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Zn2+ |
00 |
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+ 4Zn2+ |
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0036 K,Q 36 K,Q |
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- 4Zn2+ |
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Zn2+ |
151 K,Q |
Zn2+ |
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<l>epMeHT He aKTll!BeH |
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AnKOfOilbAerMAPOreHa3a |
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<l>epMeHT aKTll!BeH |
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PMc. 2-5. Poni. MOHOB 4MHK8 e cTa6MnM3&4MM 'leTeepTM'IHOMCTPYKTYPbl anKoron1.AerMAporeHaab1.
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mtte B Kaqecrne 3JieKTpoqmJIOB. 3TO, B nepsyro oqepe,llh, TaKHe MeTaJIJihl, KaK Zn2+' Fe2+' Mn2+' Cu2+. HoHhl meJIO'iH0-3eMeJihHbIXMeTaJIJJOB, TaKHe KaK Na+ 11 K+, He o6Jia,llaIOT 3THM csoH:cTBOM.
B Ka'ieCTBeIIPHMepa MO)l(HO paCCMOTPeTh $yHK1IHOHHPOBam1e <t>epMeHTa Kap6oaHfH,llpa3hl. Kap6oaHrH,llpa3a - U:HHKCO,llep)l(allIHM $epMeHT, KaTaJIH3HPYIOIIIHH peaKIIHIO o6pa30Bamrn yroJihHOH KHCJIOThI:
co2 + Hp ~ H2C03"
Hott Zn2+ B pe3yJihTaTe 3JieKTpo$HJihHOtt aTaKH yqacrnyeT B o6pa3oBaHHH ff+ H OHHOHOB H3 MOJieKYJihl BO,llhI:
H |
H |
E- Zn2+ + 0 |
-.=:::!- E - Zn~:~---·6 + H+ |
/ |
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'H
IlpOTOH H fH,llpOKCHJihHasI rpynna IIOCJie,llOBaTeJibHO IIPHCOe,llHIDllOTCH K ,llHOKCH,lly ymepo,lla c o6pa30BaHHeM yronhHOH KHCJIOTbI (CM. cxeMy A).
B XO.Ile 3JieKTpo$HJihHOro KaTaJIH3a HOHbl MeTaJIJJOB qacTo yqaCTBYIOT B CTa6HJIH3au;m1 npoMe- )J()'TO'iHbIXcoe;a:HHeHHH.
YlfaCTHe s OKHCJIHTeJ11tuo-eoccrauoBHTeJILHhlX
peaKUIDIX
HoHbl MeTaJIJJOB c nepeMeHHOtt saneHTHOCThIO
MOryT TaIOKe yqaCTBOBaTb B nepeHoce 3JieKTpOHOB.
HarrpHMep, B llHToxpoMax (reMco;a:ep)l(aIIIHX 6en-
Kax) HOH )l(eJie3a cnoco6ett npttcoe;a:ttIDITb tt oT;a:a- BaTh O,llHH 3JieKTpoH:
-e·
Fe2+ -.=:::!- Fe3+
+e·
Enaro,llapH 3TOMY csottcrny U:MTOxpoMbI yqacTBy-
JOT B OKHCJIHTeJibHO-BOCCTaHOBMTeJibHblX peaKllllSIX. ,Upyro:H npttMep yqacTMH MOHOB MeTaJIJIOB B
OKHCJIMTeJibHO-BOCCTaHOBMTeJihHblX peaKIIHHX - pa6orn <t>epMema ;a:o$aMMHfHJlPOKCHJia3bI, KamJIH3HPYIOIIIero peaKU:MIO o6pa30BaHMH HOpa,llpeHaJIMHa IIPM yqaCTllll BMTaMHHa c (CM. cxeMy 6).
3a OKMCJIHTeJibHO-BOCCTaHOBHTeJibHbie CBOHCTBa y ,llO$aMMHfHJlPOKCHJia3bl orneqaeT HOH Me,llll (puc. 2-6).
<l>epMeHT, co,llep)l(allIHH HOH Cu2+, He sczynaeT B peaKIIHIO c MOJieKYJIOH KllCJIOpO,lla. Ilptt BOCCTaHOBJieHHll Cu2+ ;a:o Cu+ c noMOIIIbIO acKop6HHOBOH KHCJIOTbl o6paJyeTCH HOH Me,llH, cnoco6Hblll B3aHMO,lleHCTBOBaTb c KMCJIOpO,llOM c o6pa30BaHHeM nepeKHcHoro coe,llHHeHHH. Aanee rH,llpoKCHJibHasi: rpynna nepeHOCHTCH Ha MOJieKYJIY .llO$aMHHa c o6pa30BaHtteM HOpa,llpeHaJIHHa.
4. PoJlb MeraJI.JIOB B perym1UHK aKTKBHOCTH
c)>epMeHTOB
Httor,lla HOHbl MeTaJIJJOB Bb1czyna10T B poJitt peryJIHTOPHhlX MOJieKYJI. HanpttMep, HOHbI Ca2+ cny- )l(aT aKTttBaTopaMtt $epMeHTa npoTeHHKHHa3bI C,
H |
0 = C =0 |
-.=:::!- |
H |
+ H -.:::!- |
E- Zn~!___ -6 + H+ + |
E- Zn----- 0 |
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2+ • / |
+ |
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~ (/,/ |
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0 |
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O==C==O' |
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2+ |
II |
-.:::!. |
E - Zn2+ + H2COa |
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E-Zn-------- 0 - c -OH |
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I |
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CxeMaA |
H |
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¢t |
02 |
H20 |
¢tH |
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CH2 |
H46n0 ~H26n |
CH-OH |
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I |
AcKop611HoeaR |
,Qer111ApoacKop611HoeaR |
I |
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CH2 |
KlllCilOTa |
KlllCilOTa |
CH2 |
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I |
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I |
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NH2 |
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NH2 |
CxeMa 6 |
,QocpaMlllH |
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HopaApeHan111H |
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O=C~ |
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0=<(1 |
1. |
2E-Cu2+ + HO-?H 0 µ |
2E-cu+ + |
O=C |
I 0 +2W |
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HO-<(H I |
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O=yj |
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HC_J |
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HC |
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I |
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I |
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HO-CH |
HO-CH |
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I |
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I |
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H2C-OH |
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H2C-OH |
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AcKop611 HOBaR |
,Qer11ApoacKop611HoBaR |
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Kl1CJlOTa |
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Kl1CJlOTa |
2. |
E-Cu+ + 0 2 + H+ µ |
E-Cu-0-0-H |
3.
HopaApeHa1111H
PMC. 2-6. Yl.faCTMe MOHS M8AM B aKTMBat.1MM Monet<ynbl KMcnopOAa npM rj>yHKl.IMOHMpoeaHMM Aorf>BMMHrMAPOKCMn83bl. 1 - BOCcraHoBneHMe Cu2•, BXOAfll.llero e cocrae aKTMBHOro 1..1eHrpa Ao¢aMMHrMAPOKc1o1naab1, AO Cu• c noMOl.llblO acKop6MHOBOM KMCflOTb1;
2 - B3al1MOABMCTBM8 Cu·c KMCflOPOAOM c o6pa30BaHMBM nepeKMCHOro cOeAMHBHl1fl; 3 - nepeHOC rMAPOKCMflbHOM rpynnbl Ha MOneKYny Ao¢aMMHa c o6paaoeaHMeM Hopai:ipeHanMHa.
KaTaJIH311py10mero peaKU1111 cl>occl>op11JI11posamrn 6eJIKOB (cM. pa3,n;eJI 5). HoHhI Ca2+ TaIOKe 113Mem110T aKTl1BHOCTb p51)1,a Ka.JibU11H-KaJibMOJJ;yJI11H3aBl1- C11MblX cl>epMeHTOB (CM. no.n;pa.3,lleJI V).
6. Ko<t>EPMEHThI
KaK }')Ke6hmo cKa3aHo, wrn npo.SIBJieH11H KaTaJil1TH'IecKoH: aKTl1BHOCTH 60.TiblllHHCTBY cl>epMeHTOB He06XO.Il.11MO Ha.JI11q11e Kocl>epMeHTa. I1CKJIIOqeH11e COCTaBJI.SllOT fl1.Il.POJil1Tl1qecK11e cl>epMeHTbl (Hanpu:Mep, npoTea3bI, JI11na3hI, pu:6oHyKJiea3a), BbinOJimn01u11e CBOIO cPYHKU11IO B OTCYfCTBl1e KOcl>epMeHTa.
Kocl>epMeHT, JIOKa.Jil13Y.SICh B KaTaJI11Tu:qecKoM yqacTKe aKTHBHoro ueHTPa, npu:H11MaeT Henocpe,llcrneHHoe yqaCTl1e B X11Ml1qecKOH peaKUHH, BbICTYna.SI B KaqecTse aKUenTopa H .n;oHopa XHM11qecK11X rpyrrnu:posoK, aTOMOB, 3JieKTpOHOB. Kocl>epMeHT MmKeT 6bITh CB.SI3aH c 6eJIKOB0H qacThIO MOJieKYJlbl KOBa.JieHTHbIM11 H HeKOBa.JieHTHblMl1 CBSI3.SIMl1.
B nepBOM cJiyqae OH Ha.3hIBaeTCSI npocTeTu:qecKotl rpyrrnotl (Hanp11Mep, FAD, FMN, 6110THH, 1mnoeBa.SI KHCJIOTa). BMecTe c TeM 113BeCTHhr np11Mepb1,
Kor.n;a Kocl>epMeHT np11coemmHeTcH K <t>epMeHTY HeKOBa.JieHTHblMl1 CBSI3HMH HaCTOJlhKO npoqHo, qTQ
He ,u:11ccou:u:11pyeT OT 6eJIKOBOH MOJieicyJibI, Hanpu:- Mep THaMllli,llJl.cPOC<t>aT.
Bo BTOPOM CJiyqae Kocl>epMeHT B3al1MO,lleHCTBYeT c cl>epMeHTOM TOJlhKO Ha BpeMH XHM11qecKOH peaKU11H 11 MO)l(eT paccMarpmmThc.SI B Kaqecrne HrO- poro cy6cTpaTa. Tipu:MephI -
Anocl>epMeHT o6ecneqHBaeT crreu11cl>mttocTh .n;etlCTBHH 11 OTseqaeT 3a BbIOOp Tl1fla XHMH:qeCKOfO npeBpameHHSI cy6cTpaTa. 0,1111H 11 TOT )l(e Kocl>epMeHT, B3a11Mo,u:eH:cTBYH c pa3.Jlu:qHhIM11 arro<PepMeHTaM11, MO)l(eT yqacTBOBaTb B pa.3HbIX x11M11qecK11X npespaIUeHHSIX cy6cTpaTa. HarrpttMep, rr11p11.IJ.0KcaJibQ>occl>aT B 3aBHCHMOCTl1 OT Toro, c KaKHM arrocl>epMeHTOM B3aHMO,lleHCTByeT' yqaCTByeT B peaKUH.SIX TpaHcaMHHHpoBaHHSI 11.TIH ,L(eKap60KCHJil1POBaHHSI aMl1HOKHCJIOT.
XttMu:qecKaSI rrp11po,lla Kocl>epMeHTOB, 11x <l>YHKUH11 B cl>epMeHTaTHBHbIX peaKUH.SIX qpe3BbJqatlHO pa3Hoo6pa3HbI. Tpa,JI11u110HH0 K Kocl>epMeHTaM OTHOCSIT rrpOH3BO,L(Hbie Bl1TaMl1HOB, XOTSI IlOMHMO HHX eCTb 3Haq11TeJibHbIH KJiacc He6eJIKOBbIX COe.Il.11HeHl1H, npHHHMaIOIUHX yqacT11e B
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npOHBJieHHH KaTaJIHTJfqecKOH $yHKU:HH $ep- |
I. MexanU3M «nunz-nonz» |
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MeHTOB. |
CxeMaTHqHo MexaHH3M |
«IIHHr-rroHr» MO)[(eT |
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K Ko$epMeHTaM OTHOCHT cne.JlYIOlll.lle coerorneHHH: |
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6bITb rrpe,!1,CTaBJieH CJie,ll,YIOII(HM o6pa30M: |
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npOH3BO,ll,Hbie BHTaMHHOB; |
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reMbI, BXOMIIIHe B COCTaB U:HTOXpOMOB, KaTa- |
P1 |
B |
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Jia3bI, nepoKCM,Ll,a3bI, ryaHHRaTU:HKna3bI, NO- |
E + A -- t EA l |
E'l |
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CHHTa3bI H HB.illllOIIIHeCH npocTe~eCKOH rpyn- |
E'B-- t P2 + E |
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noH $epMeHTOB; |
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ttyKneOTH,lJ,bI - ,ll,OHOpbI H amenTOpbl OCTaTKa |
Cy6cTpaT A, B3filfM0,!1,eHcTByH c $epMeHTOM (E), |
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$oc$opHOH KHCJIOTbI; |
rrpeBpaIIIaeTCH B rrpo,!J,yKT (P,). <l>epMeHT OCTaeTCH B |
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yfiHXHHOH, HRH KO$epMeHT Q, yqaCTBYIQII(HH |
pe3yJibTaTe 3T0f0 rrpeo6pa30BaHHH He B HaTHBHOH |
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B nepeHoce ~meKTpOHOB H npoTOHOB B UIT3; |
$opMe, a B H3MeHeHHOH (E')B pe3yJibTaTe MO,ll,H$H- |
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• $oc$oa,n;eH03HR$oc$ocynh$aT, yqacTBYIQII(HH |
Kau:HH Ko$epMema. )],anee K aKTHBHOMY u:eHTpy E' |
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B nepeHoce cynh$aTa; |
rrpHcoe,ll,HHHeTCH cy6cTpaT B, rro,!1,BepraIOII(HHCH rrpe- |
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S-a,n;eH03HnMeTHOHHH (SAM) - ,ll,OHOp Me- |
o6pa3oBaHHIO B npO,!J,yKT (P2) c BbICB060)K,Ll.eHHeM |
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THRbHOH rpynnhI; |
HaTHBHOH $opMbI $epMeHTa (E). |
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myTaTHOH, yqaCTBYIQIIIHH B OKHCJIHTeJibHO- |
XopolllHH rrpHMep MexaHH3Ma «IIHHr-rroHr» |
- |
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BOCCTaHOBHTeJibHbIX peamHHX. |
pe~ TpaHcaMHHHpoBaHHH c yqacTHeM $epMeH- |
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CTpoeHHe H $yttKU:HH 3THX Ko$epMeHTOB no,ll,- |
TOB aMHHOTpaHc$epa3 (Ko$epMeHT IIHpH,!1,0KCaJib- |
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po6Ho pacCMOTpeHbl B COOTBeTCTBYIQII(HX pa3,!1,e- |
$oc$aT). AMHHOTpaHc$epa3bI, OTKpbITbie OTe- |
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Jiax yqefiHHKa. |
qecTBeHHbIM yqeHbIM AE. IipayttlllTeHHoM, KaTaJIH- |
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3HPYIOT o6paTHMbie peamHH rrepeHoca aMHHorpyn- |
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B. MYJihTHCYBCTPATHhIE PEAKQHH |
nhI c aMHHOKHCJIOThI Ha KeTOKHCJIQTY. MexattH3M |
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«IIHHf-IIOHf» ,ll,aHHOH peamHH cxeMaTJfqHo rrpe,!1,- |
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bOJiblllHHCTBO $epMeHTOB KaTaJIH3HpyeT peaK- |
CTaBJieH Ha pHC. 2-7. |
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U:HH, B KOTOPbIX yqacTByeT 6onee qeM 0,!1,HH cy6- |
)],pyroii npHMep MexaHH3Ma «IIHHrIIOHf» |
- |
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cTpaT. B cnyqae ecnH KO$epMeHT He HBnHeTCH |
peaKU:HH ,ll,erH,!1,pHpoBaHHH c yqacTHeM KO$epMeH- |
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npocTeTHqecKoH rpynnoH:, ero TaIOKe MO)[(HO pac- |
Ta FAD ( $naBHHa,n;eHHH,!1,HttyK.JieoTM) HRH FMN |
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cMaTpHBaTb KaK eme O,ll,HH cy6cTpaT. Cne,!J,OBa- |
($JiaBHHMOHOttyKJieOTM,L1,), KOTOpbie rrpoqHo CBH- |
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TeJibHO, yqaCTHHKOB $epMeHTaTHBHOH peaKQHH |
3aHhl c $epMeHTOM H, CJie,ll,OBaTeJibHO, He MOryT |
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MO)[(eT 6bITb HeCKOJibKO: Henocpe,!1,CTBeHHO $ep- |
paccMaTpHBaTuCH B Kaqecrne BToporo cy6cTpaTa. |
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MeHT, HeCKOJibKO cy6cTpaTOB H KO$epMeHT. |
CxeMaTmHo CTpYKTYPa 3THX Ko$epMeHTOB H co- |
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B 3THX cnyqanx MexaHH3M $epMeHTaTHBHOH |
OTBeTCTBYIQIIIHe HM XHMHqecKHe $OPMYJibl rrpe,!1,- |
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peaKU:HH, KaK npaBHJIO, MO)[(eT H,ll,TH no 0,!1,HOMY |
CTaBJieHbl Ha PHC. 2-8. |
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H3 ,ll,Byx nYTeH: no MexaHH3MY «nHHr-noHr» (Me- |
FMN H FAD yqacTBYIOT B OKHCJIHreJibHO-BOC- |
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xaHH3MY ,ll,BOHHOfO 3aMell(eHHH) MJIH IIOCJie,ll,O- |
crnHoBHTeJibHbIX peaKU:HHX, aKUeIITHPYH 2 e- H |
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BaTeJibHOMy. PaccMOTPHM o6a MexaHH3Ma. |
2 H+ BH30aJIJiaKCa311HOBOMKOJlbUe (cM. cxeMY HH)[(e). |
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0 |
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H |
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II |
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o ."'cYyVNH |
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+ H3CYyNY'NH |
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HC~NAN~O |
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HC~NAN~O |
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3 |
I |
3 |
I |
I |
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R |
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R |
H |
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FAD (FMN) |
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FADH2 (FMNH2) |
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OKHCJ10HHaff ct>opMa |
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BOCCTaHOBJ10HHaff ct>opMa |
88
1j/a-KK,
PMc. 2-7. Co6b1TMH e SKTMBHOM 1..1eHTpe 8MMHOTpaHc<j)epa3bl KSK npMMep MeX8HM3M8 «nMHr-noHr>>. KocpepMeHT m1p111i:ioKcanbcpoccpaT (nCD), CB5l3aHHbli1 c cpepMeHTOM, npVIHVIMaeT a-aMVIHOrpynny OT nepsoi.1 aMVIHOKVICnOTbl (AK,), KOTOpas:i nplll 3TOM npespalljaeTCs:l B a-KeTOKVICnory 1 (KK,) VI BblCB060)l(,QaeTC5l Vl3 aKTVIBHOro l\8HTpa cpepMeHTa. ,Qanee B aKTVIBHbii.1 l\8HTP cpepMeHTa npVIC08AVIH5l8TC5l a-KeTOKVICnorn 2 (KK2), KOTOpas:i 3a6V1paer aMVIHOrpynny OT KocpepMeHTa VI npeapalljaeTCs:l B a-aMVIHOKVICnOTY (AK2).
CxeMy peaKu1111 ).lem).lp11posaH115l (KaK np11Mep MexaHl13Ma «n11Hr-n0Hr» c ylfacT11eM KoQ:>epMeHTOB FMN 11 FAD) MO)l(HO npe).lCTaBHTb B cJleJ.lyJOmeM Btt).le:
B
2. IlocJ1eiJ0Bame.nbHb1ii Mexanll3M
B CJlylfae nocJle).loBaTeJlbHoro MexaHl13Ma ).lJ151 npOTeKaHH51 Q:>epMeHTHOti: peaKUHH Tpe6yeTC51 0).l- HOBpeMeHHO B3aHMO).leHCTBHe LlBYX cy6cTparoB. B 3TOM cJlylfae B03MO)[(HO np11coe).l11HeH11e cy6CTparoB LlBYM51 pa3Jll1lfHb!Ml1 nyrnMH:
MexaHH3M ynopAAO'lettuoroB3aHMo)J,encTBm1 cy6crpaTa c aKTHBHblM 11euTpoM cj>epMeHTa:
me AH 2 |
- |
).lOHop BO).lopo).la, OKHCJlHeMbitt cy6- |
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CTpaT |
1; |
A |
- |
OKl1CJleHHa51 |
Q:>opMa cy6cTparn |
1; |
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B - |
aKuemop BOLlOPOLla - |
cy6cTpaT |
2; |
BH 2 |
- |
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BOCCTaHOBJleHH351 Q:>opMa cy6cTparn 2; |
E |
(FAD), |
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E (FADH7) |
- |
OKHCJieHH351 |
H BOCCTaHOBJleHH351 |
Q:>opMbI KoQ:>epMeHTa FAD, BXOLlHIUero s cocrns Q:>epMeHTa E.
B KalfecTBe np11Mepa FAD-3as11c11Moti peaKu1111 MO)[(HO np11BeCTl1 CYKUl1H3T).lem).lporeH33HyJO pe3KUHIO. B 3Toti: peaKu1111 B KalfecTBe srnporo cy6CTparn ylfacTByeT y611x11HoH - O).lHH 113 nocpe).l- HHKOB UCT3 (cM. cxeMy Ha c.
CTepBbIM B 3KT11BHb!H UeHTp Q:>epMeHTa np11COelll1H5leTC5l cy6cTpaT A, o6Jierlfa51 np11coe- D:HHeH11e cy6crparn B. CTOCJle XHMl1lfeCKOH MOlll1cPHKaUHH TaK)[(e Ha6Jll01l3IOT orrpe).leJleHHblH nop51).l0K BbICB060)l().leHirn nponyKTOB peaKUH11.
MexattH3M CJiy'laH:uoroB3aHMO)J.eitcTBHH cy6cTPaTa c aKTHBHblM u,euTpOM cj>epMeHTa:
E + A----- |
> |
EA + B"-. |
5- EP2 - |
E + P2 |
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EAB--- |
+ EP1P2 |
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E + B ----- |
> |
EB + A/' |
~ EP1 - |
E + P1 |
89
A |
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<1>11ae111H |
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(1113oa1111aKca3111Hoeoe |
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KOilbl..10) |
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AAeH111H |
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P1116oqmae111H |
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(BlllTaMlllH 82) |
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® -+ |
----- |
+-® |
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FMN (cp11ae111HMOHOHyKileOT111.Q) |
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AM<I> (a.QeH03111HMOHocpoccpaT) |
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r-------------------------------------------, |
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:--------------~---- |
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:HaCUNXN, |
NH |
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I |
" |
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iH3C~ |
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N~NAO |
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I |
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HC-H |
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I |
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HC-OH |
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I |
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HC-OH |
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I |
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:0 |
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HC-OH 0 |
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I |
II |
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111 |
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H2C-0-P-0-,P-0- CH2 |
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I |
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11 |
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OH |
pH |
I I I
I
OH OH
--------------------~
PMc. 2-8. CTPYKTYPB (A) MxMMM'4eCKOecTpoeHMe (6) KO(j>epMeHTOB FMN M FAD.
coo-
l
CH
II
CH
too·
<l>yMapaT
Y6111XHHOI1
90
Ilp11op1nerHocr11 3a B3a11MoneitcrB11e cy6crparoB A 11 B B aKT11BHOM u:ettrpe <PepMeHra Her (KIDK,ll,bIH cy6crpar 11Meer CBOH u:eHrp CB.H- 3bIBaHl1.Sl B aKTl1BHOM u:eHrpe). TaIOKe Her crporo:H 3aKOHOMepHOCTl1 BbICB06mK,D,eHH.Sl rrponYKTOB peaKu:1111.
Ilp11MepoM IIOCJie,D,OBareJibHOro yrrop.SlL(O'leHHOro MexaH113Ma MO)l(er 6hITb peaKu:11.11 ner11np11p0Batt11.11 c yqacr11eM Ko<PepMeHTOB NAD+, NADP+.
CxeMaTH'lHOcrpYKTYPa 11 XHMH'lecKHe<l>oPMYJibI 3TI1X KO<l>epMeHTOB rrpe,D,CTaBJieHbl Ha p11c. 2-9.
A
06a KO<l>epMeHTa <l>YHKIJ:110Hl1PyIOT KaK rrocpe,D,- Hl1Kl1 rrepettoca ,D,Byx 3JieKTpOHOB 11 O,D,HOro rrporoHa or ,D,OHopa K aKu:errropy, ,D,pyroro rrporoHa - B cpeny (CM. CXeMY A Ha c. 92).
.ll:ottop u aKu:errrop He 06113areJibHO yqacrnyior B 0,D,HOM Mera60JIH'leCKOMrryr11. LJ:pyr11M11 CJIOBaMU, BOCCTaHoBJieHHa.Sl <PopMa 3TUX HyKJieOTUL(OB ne:Hcrnyer KaK o6mu:H rryJI 3JieKTpottoB, 06pa30BaHHbIH B pe3yJihTare OKl1CJil1TeJibHbIX peaKQUH, " MO)l(eT 6bITb UCIIOJib30BaHa B pa3JIU'lHbIXBOCCTaHOBl1TeJibHblX peaKuu.11x. TaKHe peaKQuu tta3hIBa10r corrp.11)l(eHHhIM11 (cM. cxeMy Ii Ha c. 92).
Hlo1KOn1H8Ml1,D,
(Blo1T8Mlo1H}
~ ®--+--!----®-~ |
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'---~~ |
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~~~ |
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Aono11H1o1Te11bH8ff |
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¢>oc¢>aTHaff |
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rpynna |
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y NADP+ |
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AMCI> (a.QeH031-1H- |
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MOHo¢>oc¢laT) |
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Ei |
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NH2 |
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NH2 |
0 |
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N:CN |
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~ |
c~ |
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~ |
I |
I |
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ONH, |
00 |
lN) |
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~N |
CH2-0-~-0-~-0-~C2 |
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I |
I |
0 |
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H |
OH |
OH |
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O |
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II |
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HOH |
OH |
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HOH |
OH (-~-OH) |
OH
PMc. 2-9. CTPYKTYPB (A) MXMMM~ecKoe cTpoeHMe (6) Ko<ilepMeHTOB NAO+ MNAOp+.
91
aC-NH, |
0NI |
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H |
//0 |
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NI |
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D + |
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R |
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R |
NAO+ (NADP+) |
NADH {NADPH) + H |
OK~cneHHSSI cpopMa |
BOCCT8HOBJleHH8SI cpopMa |
CxeMa A |
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MeHeHHH 3HepreTHKl1 Xl1MHqecKHX peaKUHH H c |
A |
TQqKM 3peHHH co6bITl1H B aKTHBHOM ueHTPe. |
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A. 3HEPfETWIECKIIE H3MEHEHJUI |
NADH + H• |
IlPH XIIMWIECKHX PEAKUIDIX |
B
CxeMa 6
rne AH2 - nottop aonopona, aoccTattoBJieHH<UI <t:>opMa cy6cTPaTa 1; A - oKMcnettttrui: <t:>opMa cy6CTPaTa 1; B - aKUemop aonopona - BTopoH. cy6CTpaT; BH 2 - aoccTaHOBJiettttaH <t:>opMa cy6CTPaTa 2; NAD+, NADH - oKMcnettttrui: 11 BoccTaHOBJiettttaH <t:>opMbI Ko<l:>epMettTa; E, u E2 - <t:>epMeHTbl.
Ll,Be <t:>epMeHTaTl1BHble peaKU11H, KaTaJIH311pyeMbie <l:>epMettTaM11 E2, corrpmKeHhI .ri:pyr c npyroM rrocpencrnoM Ko<l:>epMeHTa NAD+, cJiyxaruero B KIDK,IJ.OM H3 3THX CJiyqaeB cy6cTpaTOM. )l.TIH rrepBoro <t:>epMeHTa cy6cTpaTOM CJiyxHT OIGICJieHHaH <t:>opMa NAD, B Kaqecrne BToporo cy6cTpaTa BbICTYITaeT ,ll.OHOP BO,II.opo,II.a - rrpHMep IIOCJie,IJ.O-
BaTeJihHbIX peaKUHH, rrpO,II.YKTOM - BOCCTaHOBJieHH<UI <l:>opMa NAD, MH <t:>epMeHTa E2 - ttao6opoT.
B Kaqecrne rrp11Mepa MO.)l{HO paccMoTpeTb cJie-
.ri:y10ruHe corrpH)l(eHHhie peaKUHH (CM. cxeMy Ha rnmi,epa.Tib,II.em.ri;<t:>oc<t:>aT ·.ri:em.ri;po-
JiaKTaT.ri:emnporetta3a.
IV. MEXAHlll3M .QElllCTBllls:t
<l>EPMEHTOB
MexaHH3M .ri:eH.cTBHH <t:>epMeHTOB MOXeT 6wTh paCCMOTPeH c ,II.Byx II03HUHH: c TO'IKH3peHHH H3-
JII06ble XHMmecKMe peaKUHH rrpOTeKaIOT, IIO,ll.- qHHHHCb ,ll.BYM OCHOBHbIM 3aKOHaM TepMO,ll.l1HaM11IG1: 3aKOtty coxpatteHHH 3Heprl1H H 3aKOHY 3HTPOIIHH. Cornactto 3THM 3aKoHaM, o6ruaH 3HeprHH XHMl1qeCKOH CHCTeMbl 11 ee OKpyxeHHH OCTaeTCH IIOCTOHHHOH, rrp11 3TOM XHMHqecK<UI CHCTeMa CTpeMHTCH K CHH)({eHl1IO yrropH,II.oqeHHOCTH (yaeJiwqeHl1IO 3HTPOIIH11). Ll,JIH IIOHl1MaHHH 3HepreTHK11 XHM11qecKOH peaKUHH He,IJ.OCTaTQqHo 3HaTb 3HepreTHqecKUi1: 6aJiaHC BXO,IJ.HIUHX H BbIXO,IJ.HIU11X 113 peaKUH11 peareHTOB, Heo6XO,IJ.11MO yql1TblBaTb 113MeHeHHH 3HeprHH B rrpouecce ,IJ.aHHOH XHMHqecKOH peaKU11H H po.Tib <t:>epMeHTOB B ,IJ.HHaMHKe 3TOro rrpouecca. PaccMOTPHM peaKUHIO pa3JioxeHHH yrOJibHOH KHCJIOThI:
H2C03 ~ Hp + co2.
YroJibH<UI KHCJIOTa CJia6rui:; peaKU11H ee Pa3JIO- )({eHHH rroH,ll.eT rrp11 o6hNHhIX ycJioBH$lX, ec.TIH MoJieKYJihI yrOJibHOH KHCJIOTbl 11MeIOT 3Hepr11IO, rrpeBbllllaIOIUYJO orrpe,II.eJieHHbIH ypoBeHh, Ha3blBaeMbIH 3ttepmeH. aKTHBauHH E. (pHc. 2-10).
3ttepruei1: aKTHBaUHH Ha3bIBaIOT ,ll.OIIOJIHHTeJibHOe KOJIHqecTBO KMHeTHqecKOH 3HeprHH, tteo6xo- ,IJ.HMOe MOJieKYJiaM Beruecrna, qTo6bI OHl1 BCTYITHJil1 B peaKUHIO.
IlpH ,II.0CTH)({eHl1H 3TOro 3Heprenrtl:eCKOro 6apbepa B MOJieKYJie rrpoHCXO,ll.HT H3MeHeHHH, Bbl3hIBaIOIUHe rrepepacrrpe,II.eJieHHe XHMJfqeCKHX CBH3eR R o6pa30BaHHe HOBbIX COe,II.HHeH11H. f OBOPHT' qTQ MOJieKYJihI, 06Jia,uaioru11e E., ttaxo,II.HTCH B rrepexo.ri:- HOM COCTOHHHH. Pa3HHllY 3HeprHH Me)l(,Lly HCXO,II.- HblM pearemoM H2C03 H KOHeqHb1M11 coe.ri:HtteHHHMH H20 11 co2 Ha3bIBaIOT H3Mettem1eM CBOOO,ll.HOH
92
rm-14ep8IlbA0n1A-3-¢occpaT |
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1,3-6111c¢oc¢orm14epaT |
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~ |
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(AOHOP BOAOPOA0) |
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coo- |
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NADH + H+ |
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CH3 |
(npoMe>K}'TO'!HblH |
l |
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I |
aK4emop BOAOPOAa) |
HC-OH |
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C=O |
~ |
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I |
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I |
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CH3 |
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COOH |
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naKTaT |
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CxeMa |
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(KOH9'-IHblHaK4emop BOAOPOAa) |
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Ceo6o.QHas:i |
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3Hepri.ts:i |
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nepeXO.QHOe |
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COCTORHl!le |
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3Hepr111s:i |
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MeHee CTa- |
aKT111aa4111111 |
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6111nbHOe COCTO- |
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RHllle |
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Vl3M0H0Hlll0 cao60AHOH 3H0pn1111
6onee cTa6111nbHOe COCTORHlll0
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93