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CHAPTER 13 Obstetrical Scanning Protocol for First, Second, and Third Trimesters 279
capsularis
Gestational sac
cavity
of the gravid endometrium). Identification of the double sac sign is confirmation of the presence of an intrauterine pregnancy before a yolk sac is visualized and it rules out a pseudosac associated with
ectopic pregnancies (pregnancies that occur outside the uterus,
more often than not in a uterine tube).
Decidua parietalis
Uterine cavity
Gestational
sac
Decidua basalis and
chorion frondosum
Myometrium
Urinary bladder
Decidua
Yolk Sac
By the end of 4 weeks GA, the primary yolk sac has regressed and is
replaced by the secondary yolk sac, which is the first anatomic struc­ture visualized within the gestational sac. The secondary yolk sac provides nutrients to the developing embryo and is the initial site of blood cell development. As illustrated below, the sonographic appear­ance of the yolk sac is small and round with bright, well-defined walls and an anechoic, fluid-filled center. Identification of the yolk sac is variable; however, it can be detected as early as 5 weeks GA with trans­vaginal transducers and should be visible by 7 weeks GA using a trans­abdominal approach. A faint flickering motion seen adjacent to the yolk sac represents the neurologically active heart tissue. From 5 to 10 weeks GA the yolk sac progressively increases to a maximum diam­eter of 5 to 6 mm. By the end of the first trimester, the yolk sac shrinks and is no longer appreciated sonographically.
Yolk sac
Chorionic
Dedcidualized
endometrium
280 PART IV Pelvic Scanning Protocols
My
cavity
c
crown rump
Double Bleb Sign
When a subtle area is visualized on the periphery of the yolk sac,
the embryonic disk has distinguished itself from the embryoblast layer. The embryonic disk lies between the yolk sac and developing amniotic membrane. On occasion, these three structures are visual­ized together. During the later portion of 5 weeks GA, their sono­graphic appearance, as illustrated below, has been described as a “double bleb” or “double bleb sign” because the embryonic disk is seen lying between the thick yolk sac and thin amniotic membrane. With developmental changes, the double bleb is not detectable after 7 weeks GA.
Amniotic
membrane
ometrium
Chorionic
Embryonic disk
Yolk sa
Gestational sac
Determining Gestational Age During the Second Half of the First Trimester
Crown Rump Length
Embryonic development is rapid during 6 to 10 weeks GA. As devel-
opment continues into the second half of the first trimester, the MSD is replaced by the crown rump length (CRL) measurement that is thought to be the most accurate assessment of GA during pregnancy. At 6 weeks + days GA it is not possible to distinguish the crown and rump thus the embryonic disk length is taken for the CRL measurement.
Calipers for
crown rump
length
Head
Body
Amniotic
cavity/fluid
Limb bud
Calipers for
length
CHAPTER 13 Obstetrical Scanning Protocol for First, Second, and Third Trimesters 281
Between 6+ weeks and 8 weeks GA, the embryo’s head becomes prominently flexed making the longest axis for measurement from the neck to the rump. As seen in the image and illustration on the fac­ing page, from 8+ weeks to 12 weeks GA the embryo’s head extends, making a true crown rump long axis for measurement.
Sonographic Appearance and Development During the Second Half of the First Trimester
Amniotic Sac
By 6.5 weeks the amniotic membrane and its fluid filled sac or
cavity have enlarged enough to surround the embryo. The thin, reflective amniotic membrane encloses the developing echogenic embryo and “bathes” it in the anechoic amniotic fluid within the amniotic sac. As the trimester progresses, not only do the CRL and amniotic sac diameter increase 1 mm per day, but their measure­ments are equal. Between 12 weeks and 16 weeks GA, the chorionic cavity will be obliterated when the amniotic cavity has enlarged enough for the fusion of the amniotic and chorionic membranes. At this time, it may or may not be possible to distinguish an actual embryo crown (or cephalic pole) from a rump (or caudal pole) but the yolk sac should be seen lying outside the amniotic cavity. The yolk sac and embryo retreat from each other but remain connected by the yolk stalk or vitelline duct that eventually becomes part of the umbilical cord, the 3-vessel connection between the embryo/ fetus and mother. Also seen during this time is the tail-like append­age that is often identified at the site of the rump.
Embryonic Heart
As previously mentioned, the embryonic heart may be detected
as early as 5 weeks GA as a flickering motion. As the first trimester progresses the embryonic heart will appear small and pulsatile. The anechoic chambers, echogenic walls, and contour may be discern­able by GA weeks 11 or 12.
Skeletal System
The axial and appendicular skeletons form between the sixth and
eighth gestational weeks. The bright reflection of the fetal skeleton indicates the degree of mineralization that has taken place within the developing bones. Ultrasound is able to distinguish how ossified portions of the fetal skeleton appear highly echogenic compared with the midgray appearance of adjacent cartilaginous structures.
Umbilical Cord
During GA week 8 the embryo assumes a “C” shape, fetal limb buds
start to be visible, placental development begins, and the umbilical cord can be visualized. The cord will appear thick and about as long
282 PART IV Pelvic Scanning Protocols
herniation
as the embryo. In short axis it presents as 1 large, round, anechoic vein with bright walls, flanked by 2 small, round anechoic arteries with bright, walls. A gelatinous tissue, Wharton’s jelly, surrounds the 3 vessels within the cord and prevents it from becoming crushed. The umbilical cord will continue to grow at a rate similar to the embryo.
Tenth Week
The following image demonstrates that by GA week 10 (30 mm CRL, between measurement calipers), limbs are detectable, and normal gut herniation into the base of the umbilical cord is evident. Normal gut herniation should not be seen after GA week 12.
TRV
Amniotic sac
Umbilical cord/gut
site
Leg bud
End of the First Trimester
During GA weeks 11 and 12, individual fingers and toes can be iden-
tified as well as anechoic fluid in the stomach and urinary bladder, and the midgray, homogeneous liver. By the end of the first trimes­ter, the oral cavity including hard and soft palates and the tongue are consistently identified. The mid- and distal portions of the esophagus may occasionally be seen as 5 parallel lines anterior to the thoracic aorta. The distal esophagus is nearly impossible to see. The embry­onic head and body become proportional and the embryo has devel­oped into a fetus that assumes a distinct humanlike appearance.

Second and Third Trimesters

Using a 28-day menstrual cycle, most radiologists and obstetricians define the second and third trimesters as GA weeks 13 to 42.
Sonographic Appearance and Development During the Second and Third Trimesters
By GA week 13, the majority of organs formed during the first tri­mester are located in their final anatomic positions. During the sec­ond and third trimesters, these organs and their associated organ systems become fully developed as other body structures continue to grow and mature. To grow properly and develop normally, the
CHAPTER 13 Obstetrical Scanning Protocol for First, Second, and Third Trimesters 283
fetus depends on the placenta and umbilical cord for nutrients, oxy­gen, and removal of metabolic waste products.
Placenta
The early placenta appears medium gray with homogeneous echo
texture. It darkens slightly during the second and third trimesters and as the gestation advances, the homogeneous appearance of the placenta may be interrupted by bright, echogenic calcium depos­its and/or small anechoic lacunae (maternal venous lakes) and/or retroplacental and intraplacental arteries that appear anechoic with bright walls. Anechoic tubular structures on the uterine surface of the placenta representing maternal marginal veins may also be visualized.
Placental Grading
Many institutions utilize placental grading, a system that clas-
sifies placenta maturation according to its sonographic appear­ance (see Figure 13-1). Basically a grade 0 placenta appears normal throughout pregnancy. The chorionic plate (sac border) remains smooth; the basal layer (uterine border) is free of calcification, and the parenchyma or bulk of the placenta remains medium gray and homogeneous except for anechoic lacunae. With grade I, the chorionic plate shows some subtle indentations, the basal layer becomes hypoechoic or anechoic relative to adjacent struc­tures, and the parenchyma exhibits a few scattered, bright punc­tate densities (calcifications). These findings are considered normal any time after 34 weeks of development. A grade II
placenta pre­sents with medium-sized indentations of the chorionic plate, a few small, linear, bright densities are identified at the basal layer, and the parenchyma contains bright, scattered, “commalike” densities. These findings are considered normal any time after 36 weeks of development. With grade III, the chorionic plate shows indenta­tions extending as far as the basal layer, dividing the placenta into segments. The basal layer exhibits very long, linear, bright, den­sities that may, in advanced stages appear as a bright, unbroken line. The placental parenchyma may contain highly echogenic and anechoic areas. The bright echoes represent large calcifications that may cast acoustic shadows. These findings are considered normal any time after 38 weeks of development.
Placenta Previa
The location of the placenta is variable within the uterus and may change as the uterus expands to accommodate the growing fetus. Sonographic evaluation of the placenta includes its position relative to the cervical internal os to rule out placenta previa, a condition where the os is obstructed by overlying placenta. Depending on the
GRADE 0
PLACENTA
PLACENTA
PLACENTA
PLACENTA
GRADE III COMPONENTS
Linear basal
Placenta
Myometrium
Placental
Lacunae
GRADE 0 COMPONENTS
substance
GRADE I
GRADE II
Placenta
Placenta
Placenta
Lacunae
Punctate echoes
Subtle indentations
GRADE I COMPONENTS
Basal
Myometrium
"Commalike" densities
GRADE II COMPONENTS
echoes
Medium
indentations
Myometrium
Placental substance
Placental
substance
Myometrium
Lacunae
GRADE III
Acoustic
shadow
Irregular densities
echoes
Placental substance
FIGURE 13-1 Placental Grading. Classifications of the “aging” placenta. Notice the increase in calcifications (bright-appearing densi-
ties) and changes in contour from grades 0 to III. Grade 0: Represents the normal placenta. The placental substance appears homoge­neous with medium- to low-level echoes that may be interrupted by anechoic lacunae. Grade I: The chorionic plate begins to show some signs of subtle indentations, a few scattered densities are present, and the basal layer appears anechoic. Grade II: Medium-sized indenta­tions are evident in the chorionic plate, “commalike” densities are prevalent throughout the placental substance, and a few small linear densities appear in the basal plate. Grade III: The chorionic plate contains indentations extending to the basal layer, dividing the placenta into segments. The placental substance appears complex, with both anechoic areas and bright focal areas representing large calcifications that may cast shadows. Long, linear densities are evident in the basal plate. In advanced stages they may appear as an unbroken line.
CHAPTER 13 Obstetrical Scanning Protocol for First, Second, and Third Trimesters 285
Umbilical cord
Fetal parts
degree of blockage, it is described as marginal, partial, or complete previa. When there is partial or complete placenta previa the patient may require a cesarean delivery rather than a vaginal delivery that would be dangerous for the fetus and mother. A placenta previa can be ruled out if the placental edge is 2 cm from the internal os. The following image shows an example of partial placenta previa (PL, Placenta; CX, cervix; BL, bladder).
PL
BL
CX
(Courtesy Joe Antony, MD, from www.ultrasound-images.com, and inspired by Ravi Kadasne, MD.)
Umbilical Cord
The umbilical cord is the vascular connection between the fetus
and the placenta where fetal circulation begins. The normal umbil­ical cord is composed of a single vein flanked by 2 arteries. The ves­sel lumens appear anechoic; the surrounding walls appear bright. The umbilical cord develops multiple spiral turns as it increases in length; therefore, the 3 vessels are usually easier to distinguish in short axis sections as seen in these images
Umbilical
artery
Umbilical
vein
Umbilical
artery
Grade I placenta
286 PART IV Pelvic Scanning Protocols
Umbilical vein
Spine
Urinary bladderFemur
Umbilical Cord Insertion
The next 2 images show the umbilical cord insertion into the pla-
centa and into the fetus. After the first trimester, the fetal surface vessels are routinely visualized. As demonstrated in the top image, they can be traced to the site where the vessels merge and pene­trate the placental parenchyma. The bottom image shows that the umbilical cord can be identified where it enters or inserts into the fetus at the umbilicus. The umbilical vein runs cephalically to join fetal portal circulation. The arteries take a caudal course, running on each side of the urinary bladder to meet the iliac arteries.
Placental
insertion
Umbilical
arteries
Amniotic
fluid
Placenta
cord
Myometrium Amniotic fluid
Umbilical cord
insertion
Color flow box
Fetal limb
Umbilical cord
Placenta
Bowel
Iliac wing
Myometrium
Sonographic Appearance of Fetal Anatomy
Midgray structures: Organ parenchyma and muscles fall into a wide
range of midgray shades that provide delineation between the different structures.
Reflective structures: Bone (ossified), choroid plexus, and the menin-
ges appear highly echogenic with varying degrees of intensity.
Anechoic structures: Echo-free, fluid-filled anatomy that includes
the urinary bladder, stomach, gallbladder, blood vessels, brain ventri­cles, and heart chambers.
CHAPTER 13 Obstetrical Scanning Protocol for First, Second, and Third Trimesters 287
Skeletal System
Axial Skeleton
During the second and third trimesters most of the bones of the
axial skeleton are routinely visualized. The calvaria of the skull appear highly echogenic and prominent. The contour of the nor­mal fetal head should appear smooth and elliptical in shape. Other identifiable bones include the rib cage in the thorax, mandible, nasal ridge, orbits, and fetal spine. The vertebrae have a highly reflective appearance, making them easy to recognize. The image below shows a longitudinal section of the fetal spine that can be described as 2 rows of closely spaced reflectors on each side of the medium- to low-gray appearance of the spinal cord. The 2 rows are roughly parallel, but wider in the cervical and lumbar regions and narrower in the sacral region. The gaps between the vertebral bodies are composed of nonossified margins of adjoining vertebral bodies and the intervertebral disks. In short axis sections, the ver­tebral anterior ossification center is seen equidistant from the 2 posterior ossification centers.
Fetal Spinal Cord
(Image courtesy of Philips. [Philips acquired ATL in 1998.])
Appendicular Skeleton
During the early to midpart of the second trimester most of the
bones of the appendicular skeleton are routinely visualized. This
(From Pilu G, Nicolaides KH: Diagnosis of fetal abnormalities: The 18- to 23-week scan, Boca Raton, Fla., 1999, CRC Press. Figure 8-1, p. 88.)
288 PART IV Pelvic Scanning Protocols
includes upper and lower extremities as demonstrated in the pre­vious image. In short axis sections, they appear as bright echo­genic foci surrounded by low-gray, homogeneous soft tissue. The longitudinal sections of the bones appear linear, bright, and reflective. The femurs especially, cast a very prominent shadow. The cartilaginous ends of the bones appear homogeneous and low-gray.
Cardiovascular System
Heart
By 15 weeks GA the 4 chambers of the fetal heart can be visu-
alized. The walls of the heart appear midgray and hyperechoic relative to the anechoic blood in the chambers. The 4 chambers should appear relatively symmetric as demonstrated in the image below. They are divided by the echogenic atrioventricular septa, which will appear “broken” at the foramen ovale (a normal opening between the atria allowing blood to move right to left in the fetal heart). The heart is normally visualized on the left side of the thorax.
Atrioventricular septaLeft ventricle
Left atrium
Foramen ovale
Right atriumRight ventricle
(Image courtesy GE Healthcare.)
Blood Vessels
Blood vessels in the fetus appear as they do after birth, bright walls
and anechoic blood in the lumens. The superior vena cava, tho­racic aorta, and pulmonary artery can be visualized in the upper mediastinum during the second trimester. With advanced GA, the brachiocephalic artery, common carotids, left subclavian, and the jugular veins are frequently visualized. The abdominal aorta and inferior vena cava are easy to identify in the posterior portion of the abdomen. The iliac arteries and veins are also often observed. Color Doppler sonography is helpful for resolving small vessel