- •Биологическое окисление 1
- •Содержание
- •Биоэнергетика
- •История учения о БО
- •Antoine Lavoisier
- •Теория активации кислорода
- •Критическая оценка теории Баха-Энглера
- •Хромогены и гистогематины
- •Редокс-реакции, редокс потенциал
- •Maкроэргичность ATФ
- •ATФ-AДФ цикл
- •Образование субстратов БО
- •Stages 1 and 2
- •Stage 3
- •Ферменты и коферменты БО
- •Структура FAD и FMN
- •Мх: локализация
- •Общий план строения Мх
- •Internal structure of a mitochondrion
- •The Comparative Characteristics
- •Membrane Composition:
- •Tricarboxylic Acid Cycle
- •Krebs’
- •Role of TCA
- •Plastic Role of
- •Regulation of
- •Regulation of the TCA Cycle (cont’d)
- •Inhibitors of Krebs Cycle
- •Content
- •Introduction
- •The Ways of Oxygen Consumption
- •Biologic Oxidation (BO)
- •Biomedical Importance of BO
- •Energy Conversion: Mitochondria
- •Chemiosmotic Coupling
- •Electron Transporting Chain, ETC
- •Electron Transporting Chain (ETC)
- •ETC functions
- •ETC Complexes: Overview
- •Complex I (NADH-CoQ reductase)
- •Coenzyme Q (CoQ) or Ubiquinone
- •Complex II (Succinate-CoQ reductase)
- •Complex II and III
- •Complex IV: Cytochrome c Oxidase
- •ATP/ADP translocase
- •Respiratory Chain Functioning
- •Functional scheme of ETC
- •Inhibitors of Oxidative Phosphorylation
- •The Structures of Several Inhibitors of
- •The Sites of Action of Several Inhibitors of ETC and/or OP
- •Several Uncouplers of OP
- •Uncoupler Action
- •Endogenous Uncouplers Enable
- •P/O Ratio
- •Disorders of Mitochondrial
- •Clinical Manifestation and
- •Some Mitochondrial Diseases
- •LHON
- •MERRF, MELAS et al.
- •Can Mitochondrial Diseases be Treated?
- •Cytochromes P450 are monooxygenases important for the detoxification of many drugs
- •Cytochrome b5
- •Monooxygenase System (Microsomal
- •Functioning of Microsomal
- •Microsomal Oxidation and Cytochrome P450
Endogenous Uncouplers Enable
Organisms To Generate Heat
Certain cold-adapted animals, hibernating animals, and newborn animals generate large amounts of heat by uncoupling oxidative phosphorylation.
Adipose tissue in these organisms contains so many mitochondria that it is called brown adipose tissue for the color imparted by the mitochondria.
The inner membrane of brown adipose tissue mitochondria contains an endogenous protein called thermogenin (literally, “heat maker”), or uncoupling protein, that creates a passive proton channel through which protons flow from the cytosol to the matrix.
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P/O Ratio
Electrons that enter the chain from NADH supports the synthesis of ≈3 moles of ATP.
Electrons that enter the chain from FADH2 supports the synthesis of ≈2 moles of ATP.
The P/O ratio refers to the number of inorganic phosphate molecules utilized for ATP generation for every atom of oxygen consumed.
NADH P/O = 3
FADH2 P/O = 2
Ascorbate P/O = 1
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Disorders of Mitochondrial
Oxidative Phosphorylation
Mitochondria contain DNA (mtDNA).
Some components of ETC are coded in mtDNA. Others – in nuclear DNA.
Several disorders of OP are the result of mtDNA damage.
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Clinical Manifestation and
Treatment of Mito Disorders
Manifestation
Muscle cramping and weakness,
Fatigue,
Lactic acidosis,
CNS dysfunction,
Vision problems.
Treatment
Is difficult and often unsuccesfull
In some cases can be helpful ubiquinone, vitamin C, menadione.
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Some Mitochondrial Diseases
The names of mitochondrial diseases are often complex and usually are described by abbreviations.
LHON, Lebers hereditary optical neuropathy;
MERRF, myoclonic epilepsy and ragged-red-fiber disease;
MELAS, mitochondrial encephalomyopathy, lactic acidosis, and strokelike episodes;
NARP, neurological muscle weakness,ataxia, and retinitis pigmentosa;
Leigh disease — SNE, subacute necrotizing encephalomyelopathy;
KSS, Kearns–Sayre syndrome;
CPEO, chronic progressive external ophthalmoplegia.
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LHON
LHON is a hereditary disease that often leads to sudden blindness from death of the optic nerve especially among males.
Any one of several point mutations in
subunits ND1, 2, 4, 5, and 6 of NADH dehydrogenase (complex I),
cytochrome b of complex II, or
subunit I of cytochrome oxidase
may cause this syndrome.
Most frequent is an R340H mutation of the ND4 gene at position 11,778 of mtDNA. It may interfere with reduction of ubiquinone.
Mutations in the ND1 gene at position 3460 and in the ND6 gene at position 14484 or in the cytochrome b gene at position 15257 cause the same disease.
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MERRF, MELAS et al.
The most frequent (80 – 90%) cause of MERRF, which is characterized by epilepsy and by the appearance of ragged red fibers in stained sections of muscle, is an A → G substitution at position 8344 of mtDNA in the TψC loop of mitochondrial tRNALys.
A similar disease, MELAS, is accompanied by strokes (not seen in MERRF) and is caused in 80% of cases by an A → G substitution in the dihydrouridine loop of mitochondrial tRNALeu.
CPEO, Leigh disease, and KSS often result from large deletions of mtDNA.
NARP and related conditions have been associated with an L156R substitution in the ATPase 6 gene of ATP synthase.
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Can Mitochondrial Diseases be Treated?
Attempts are being made to improve the function of impaired mitochondria by adding large amounts of ubiquinone, vitamin K, thiamin, riboflavin, and succinate to the diet.
One report suggests that mitochondrial decay during aging can be reversed by administration of N-acetylcarnitine.
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Cytochromes P450 are monooxygenases important for the detoxification of many drugs
&for the hydroxylation of steroids
Cytochromes P450 - superfamily of heme- containing monooxgenases,
> 1000 such enzymes are known.
Both NADH and NADPH donate reducing equivalents for the reduction of these cytochromes, which in turn are oxidized by substrates in a series of enzymatic reactions collectively known as the hydroxylase cycle.
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Cytochrome b5
In liver microsomes, cytochromes P450 are found together with cytochrome b5 and have an important role in detoxification.
Benzpyrene, aminopyrine, aniline, morphine, and benzphetamine are hydroxylated, increasing their solubility and aiding their excretion.
Many drugs such as phenobarbital have the ability to induce the formation of microsomal enzymes and of cytochromes P450.
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