Новые достижения в химии и химической технологии растительного сырья. Материалы III всероссийской конференции. Книга 2 [2007]
.pdf!"#$%&%'() *+$(,-"".- /#"".-, 0+1"+ 2/-$#34 5.5+/, ,3+ 52- %22$-/(-0.- 2+-/%"-"%) )5$)632) 788-93%5".0% #"3%+92%/#"3#0%. :#%;+$-- #93%5".0 5 +3"+<-"%% DPPH '#/%9#$# )5$)-32) 9#3-=%", #"3%'#/%9#$4"#) #93%5"+234 2%'%">+5+? 9%2$+3. % 0#3#%'-&%"+$# *'#93%,-29% +/%"#9+5..
!"#$%": @ 7923'#93- +;+$+,9% 2-0)" P. sibirica 0-3+/+0 ABC +;- "#'(1-". 8-"+$4".- 2+-/%"-"%), "#=+/)D%-2) 5 0%"+'".= 9+$%,-23- 5#=, "+ *'+)5$)6D%= 5.2+9(6 #"3%'#/%9#$4"(6 #93%5"+234 5 +3"+<-- "%% DPPH '#/%9#$#. E'-/*+$+1%3-$4"+, 73% 2+-/%"-"%) +3"+2)32) 9 >'(**- +$%>+- % *+$%0-'".= *'+F%#"%/%"+5 % $%>"#"+5. G23#"+5%$%, ,3+ #"3%'#/%9#$4"#) #93%5"+234 (5-$%,%5#-32) 5 ')/( #29+';%"+5#) 9%2$+3# – 2%'%">+5#) 9%2$+3# – 0#3#%'-&%"+$ – 7'%+/%93%+$ – 9#3-=%".
!"#$%'%&
1.H+20#" @.I., E+1#'%F9#) J.:., K-"9-5%, L.M. % /'. J2+;-""+23% =%- 0%,-29+? =#'#93-'%23%9% 7923'#93%5".= 5+/+'#235+'%0.= 5-D-235
Pinus Sibirica Du Tour // N#23%3-$4".- '-2('2.. 2001. O. 4. B. 81–89.
2.E+1#'%F9#) J.:., P%9+5 !.:., Q##92+ L. % /'. E+$%8-"+$4".- 2+- -/%"-"%) 2-0)" 2+2". Pinus sibirica Du Tour // N#23%3-$4".- '-2('2.. 2007. O2. B. 39–46.
3.Pozharitskaya O.N., Ivanova S.A., Shikov A.N., Makarov V.G. Separation and evaluation of free radical-scavenging activity of phenol components of Emblica officinalis extract by using HPTLC-DPPH• method // J. Sep. Sci. 2007. in press.
4.Shikov A.N., Poltanov E.A., Dorman H.J.D. et al. Chemical composition and in vitro antioxidant evaluation of commercial water-soluble willow herb (Epilobium angustifolium L.) extracts // J. Agric. Food Chem. 2006. Vol. 54. P. 3617–3624.
5.Depkevicius A., van Beek T.A., Lelyveld G.P. et al. Isolation and structural elucidation of radical scavengers from Thymus Vulgaris leaves// J. Nat. Prod. 2000. Vol. 65. P. 892–896.
220
!"#$%&'%(")$#$*(+% ,%)#-+.+ #/&%-%0%(+1 *()+#."+-*()#$ 2%(#03(#4# )+/*
$ &*")+)%03(56 ,*"0*6 " +"/#037#$*(+%, $5"#.#822%.)+$(#9 :+-.#")(#9 6,*)#4&*2++
#.;. &<=>?@A, %.*. /@=@BCD>, %.*. 6@E@F@E=CD>, +.$. ;@G>EDC?@A>
!"#"$%&'()* +"',-.#'/0%$$1* .#2)/%(/,#$"-'/#")/%34$1* ,$)0%#')/%/, ,3. 20 3%/ 5(/67#6, 84, !"#"$%&, 394004 (8"'')6) E-mail: robi@vmail.ru
!"#$%&'$()"#* +,"%-."%/% #$0) 01$2,"34# &)& $"/$5$#%1* %&$'-,"$3 2)'-%6$1%7%8 01%(9&:$$, %"$ 01$'9#'#794# 7 0%-$2,1"%2 90)&%7%;"%2 2)#,1$)-,. <,&%#%1*, 1)'#$#,-."*, 2)'-) (")01$2,1, 0)-.2%7%,, 0)-.2%3(1%7%,), ()6, '7,6,7*1)5%#)""*,, '%(,16)# 5%-.- =%, &%-$;,'#7% '7%5%("*> 6$1"*> &$'-%#, &%-$;,'#7% &%#%1*> 01$ >1)","$$ 5*'#1% 7%?1)'#),#. @#% '73?)"% '% '0%'%5"%'#.4 2)',- & ')- 2%01%$?7%-."%29 /$(1%-$?9, ?) ';,# 01$'9#'#7$3 7 "$> +,12,"#%7, '0%'%5'#794A$> B++,$7"%29 1)'A,0-,"$4 #1$/-$:,1$(%7. C '73?$ ' B#$2, (-3 0%7*=,"$3 '#%8&%'#$ 01$ >1)","$$, 7 B#$ 2)'-) %53?)#,-.- "% 77%(3# )"#$%&'$()"#* ()-&$-/)--)#*, !"#!-59#$-/$(1%&'$>$"%"*, 3,5-($-!"#!-59#$--4-/$(1%&'$)"$?%-, 3,5-($-!"#!-59#$--4-/$(1%&'$- #%-9%- $ (1.). C #%6, 71,23 (%5)7&$ )"#$%&'$()"#%7 7 (%1%/$, '%1#) 1)'#$#,-."*> 2)',- ", 01,(9'2%#1,"*, ",&%#%1*, $? 0,1,;$'-,""*> )"#$%&'$()"#%7 ", (%09'&)4#'3 (-3 $'0%-.?%7)"$3 7 '01,()>. C :,-3> %5")196,"$3 +)-.'$+$&):$$ $ &%"#1%-3 &);,'#7) $ 5,?%0)'"%'#$ 2)'- -%6$1%7%8 01%(9&:$$ ) )-."%8 37-3,#'3 1)?1)5%#&) B++,$7"*> 2,#%($& %01,(,-,"$3 )"#$%&'$()"#%7 7 B#%8 01%(9&:$$. C '#)"()1#$- ?%7)""%8 2,#%($&, $> %01,(,-,"$3 7 6$1)> $'0%-.?9,#'3 ")')(%;")3 DEF. E$1* 77%(3# 5,? 01%5%0%(/%#%7&$ 7 '0,:$)-."*8 $"6,%1 (-3 9(,16$7)"$3 ",-,#9;$> 6$1%7 $-$ +%1&%-%"&9, ?)0%-",""94 '$-)"$- ?$1%7)""%8 '#,&-3""%8 7)#%8 $-$ '#,&-3""*2$ =)1$&)2$ $ ")/1,#94 (% 250 °G. H,1$%($;,'&$ +%1&%-%"&) ?)/13?"3,#'3 6$1)2$ $ ")0%-"$- #,-. +%1&%-%"&$ ",%5>%($2% 2,"3#.. H1$2,","$, 0132%/% 77%() 6$- 1%7 7 '-9;), C@EF #)&6, 01$7%($# & 5*'#1%29 ?)/13?","$4 01,(&%- -%"%& $, 7 &%",;"%2 ';,#,, 92,".=),# 71,23 6$?"$ %'"%7"*> &%-%"%&. C 1,6$2, %51)A,""%-+)?%7%8 C@EF (-3 9'0,="%/% B-4$1%7)"$3
221
!"#$%#&'"#()* +'"', -)%).-/ !"'0/'!12 3"4-!#+'1-# 0',*)(.56 4&'!)-
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
.)$) !#34. > 1*2,# 1 9!#7 (%2 3)*5A'.#2 +/*1!*#!'%?.)1!# 7'!)(#-# 4-!/4%?.)6 ,4(4+'6 2*%2'!12 -).&'.!"#")*4.#' 8'.)%)*. B(.#7 #, *),7)=.5: "'A'.#6 3'"'+#1%'..5: 3")0%'7 2*%2'!12 9-1!"4-)..42 3")0)3)($)!)*-4. C%)=.)1!? '' "'A'.#2 ,4-%;+4'!12 * !)7, +!) $#(- ")8)0.5' 8'.)%?.5' 4.!#)-1#(4.!5 3%):) "41!*)"#75 * 3)%2".5: "41!*)"#!'%2: # !"/(.) #,*%'-4;!12 #, =#")*)6 74!"#&5.
D'%? .41!)2<'$) #11%'()*4.#2 – "4,"40)!-4 13)1)04 #,*%'+'.#2 4.!#)-1#(4.!)* 8'.)%?.)$) !#34 # ("/$#: 8'.)%)* #, "41!#!'%?.5: 741'%, )3!#7#,4 4%$)"#!74 3")0)3)($)!)*-# # 4.4%#,4 8'.)%)* 1 3"#7'.'.#'7 7'!)(4 #,)-"4!#+'1-)6 )0"4<'..)-84,)*)6 >EFG.
> -4+'1!*' )0H'-!)* #11%'()*4.#2 *,2%# 8'.)%, !-, "-, #- -"',)%5, !-$%&$-0/!#%8'.)%, 4-7'!#%-2,6-'($%&$-0/!#%8'.)%. G")74!)$"48#- ")*4.#' *53)%.2%# .4 3"#0)"' Gilson ()(.).41)1.56 *4"#4.! 1 .41)-
1)7 302 Piston Pump # ('!'-!)")7 HM Holochrome UV/Vis). I%2 **)(4 3")0 * :")74!)$"48 3"#7'.2%# #.='-!)" # Rheodyne 7125 1 3'!%'6 *7'1!#7)1!?; 20 7-%.
@47# 43")0#")*4.5 9-1!"4-)..5' 1#1!'75 9!4.)%?.56 "41!*)" JB@, #,)3")34.)%?.5' "41!*)"5 JB@ # NH4OH. @46('.), +!) * 13#"!)- *5: "41!*)"4: JB@ * "',/%?!4!' "'4- )75%'.#2 )0"4,/'!12 /1!)6+#*42 97/%?1#2, ,4!"/(.2;<42 3")0)3)($)!)*-/. K"# #13)%?,)*4.## #,)3")34-
.)%?.)$) (LKC) "41!*)"4 NH4OH )0"4,)*4.#' 97/%?1## .' )!7'+'.). M4- -#7 )0"4,)7, 3"#7'.'.#' 9!)$) "41!*)"4 7)='! 05!? #13)%?,)*4.) (%2 #,*%'+'.#2 8'.)%)* #, =#")*)6 84,5 3"# "@=9-10. B(.4-) <'%)+.5' "41!*)"5 "4,"/A4;! 1)"0'.!5 .4 )1.)*' 1#%#-4$'%2, 3)9!)7/ * 3")0)3)(- $)!)*-' - 4.4%#,/ 7'!)()7 >EFG .')0:)(#7) 3"'(/17)!"'!? ()3)%.#- !'%?./; 1!4(#;. I%2 9!)$) #,)3")34.)%?./; 84,/ 1%'(/'! "41!*)"#!? * *)(' # 3)(-#1%#!? 1'".)6 -#1%)!)6 () "@=2–3. > 9!)7 1%/+4' 8'.)%5 3'- "':)(2! * 7)%'-/%2"./; 8)"7/. N1%# 3"#7'.#!? 988'-! *514%#*4.#2 3/!'7 ()04*%'.#2 * 1#1!'7/ «*)(4 – LKC» .')0:)(#7)$) -)%#+'1!*4
222
!"#$%&'& &(()*+,, +-.' /&!!#).*+. *& -0. %&12 3 0)-*)-!)#.0"4 + +1)- 5/)5&*)#$*"4, 5)!#.-*,, !)-./6+' ~ 2 )7. % 0)-2. 8 9')( !#":&. ()#.- ;"#,/*&, %)/(& %.*)#& + )!'&'):*). ;)#+:.!'0) 6+/)0)< %&12 )!'&.'!, 0 +1)5/)5&*)#$*)( !#)., ;)')/2< "6. 5/+=)-.* -#, &*+1&.
>&<-.*), :') 0.#+:+*& )-*);/&'*)< !'.5.*+ +10#.:.*+, /&!!(&'- /+0&.(2? %.*)#)0 +1 0)-*)-!)#.0)=) /&!'0)/& +1)5/)5&*)#$*)< %&1)< (R>98 %) 5)-?)-+' -#, 5/)0.-.*+, &*+1& (.')-)( 8@AB ('&7#. 1.). C/+ 9')( ;)(5$4'./*2. /&!:.'2 5&/&(.'/)0 ()#.;"# %.*)#)0 5);&- 120&4', :.( 7)#$D. =+-/)%)7*)!'$ (lgP) + /&-+"! 6+/&E++ (0/&F.- *+,) %.*)#$*)=) !).-+*.*+, (RG), '.( 02D. *&7#4-&.(2< 9;!5./+(.*- '$*) ;)9%%+E+.*' /&!5/.-.#.*+, D. @') )7"!#)0#.*) 5)02D.*+.( 5)0./?*)!'*)< &;'+0*)!'+ %.*)#)0 + "0.#+:.*+.( +? ?+(+:.!;)=) !/)-!'0& ; )/=&*+:.!;)< %&1..
G&1/&7)'&**2< =)/+'( ?/)(&')=/&%+:.!;)=) !5)!)7& )5/.-.#.- *+, %.*)#$*2? &*'+);!+-&*')0 0 /&!'+'.#$*)( (&!#. 1&;#4:&.'!, 0 !#.-"4F.(.
!"#$#%#&'#(#)*+, ,-.#)/0 / 1#& +2+./3+. H 10 (# &*+1+/".()=) 5)-!)#*.:*)=) (&!#& -)7&0#,#+ 10 (# +1)5/)5&*)#$*)=) /&!'0)/& NH4OH ! ;)*E.*'/&E+.< 7.1 ()#$/# + 9;!'/&=+/)0+ *& 0+7/)!(.!+'.- #. 0 '.:.*+. 15 (+*. I&'.( 5)#":.**2< /&!'0)/ E.*'/+%"=+/)0+ 0 '.:.*+. 5 (+*, 0./?*44 +1)5/)5&*)#$*"4 %&1" )'-.#,#+ + 5)(.F+ 0 (./*2< E+#+*-/ 0(.!'+()!'$4 50 (#, 5/+#+0+ 10 (# 0)-2, 5)-- ;+!#,#+ !./*)< ;+!#)')< -) /> ~ 2–3 + -)7&0#,#+ !"#$%&' &(()*+, -) 40% (&!!. C)#":.**2< /&!'0)/ 9;!'/&=+/)0+ *& 0+7/)!(.!+'.#. 0 '.:.*+. 15 (+*, & 5)!#. 9;!'/&;E++ E.*'/+%"=+/)0+ 0 '.:.*+. 5 (+*. 8./?*+< !#)< E.*'/+%"=&'& )'7+/+ + ?/)(&')=/&%+/)0+ *& ?/)(&')=/&%. ! JK--.'.;')/)( (.')-)( )7/&F.**)-%&1)0)< 8@AB. L)/7.*' Nucleosil 100-C18, /&1(./ :&!'+E 5 (;(, /&1(./ ;)#)*;+ 4×250 ((. 8/.(.*& "-./6+0&*+,: %.*)# – 2,5 (+*, ;/.1)#2 – 2,7 (+*,
#-("4(-7"'+#%.*)# – 4,1 (+*; +)*)# – 6,5 (+*, &*+'+:.!;&, -#+*& 0)#*2 274 *(; 5)-0+6*&, %&1& 0)-& – &E.')*+'/+# (1 : 4), /&!?)- 5)-- 0+6*)< %&12 1 (#/(+*; )7M.( 00)-+()< 5/)72 5 (;#; 0/.(, &*+1& ! 5/)7)5)-=)')0;)< ~ 55 (+*.
223
!"#$%&" 1. '%()*+*#,*-./ +0,*$*1 (lgP), )"(%2- 3%)"&%% (RG, ,4), 5*6++%&%0,.7 )"-8)0(0$0,%9 (D) % -.080,% %:1$0;0,%9 +0,*$*1 %: 1*(,*--*$01*<* )"-.1*)" -2$/+"." "44*,%9 %:*8)*8%$*174 -8%).*4 (R,% )
=0,*$/,*0 -*0(%,0,%0 |
lgP |
RG |
D |
R |
+0,*$ |
1,64 |
0,22 |
530 |
98,1 |
!-5)0:*$ |
2,13 |
0,24 |
590 |
98,3 |
"-5)0:*$ |
2,00 |
0,25 |
610 |
98,4 |
#-5)0:*$ |
2,13 |
0,26 |
630 |
98,4 |
!-$%&$-#2.%$+0,*$ |
3,35 |
0,27 |
750 |
98,6 |
%*,*$ |
5,54 |
0,31 |
810 |
98,7 |
>*,&0,.)"&%? +0,*$*1 1 65-.)"5.0 )"--;%.71"$% 8* <)"(2%)*- 1*;,*42 <)"+%52 S=f(c+), " -*(0)3",%0 +0,*$*1 1 %-@*(,*A 8)*#0 8* +*)42$0 ' = 100'!/(Rr), <(0 ' – 5*,&0,.)"&%9 ","$%." 1 %-@*(,*A 1*(- ,*A 8)*#0, 4</(43; '! – 0<* 5*,&0,.)"&%9 1 65-.)"5.0, 4</(43; r – -**.- ,*B0,%0 )"1,*10-,7@ *#C04*1 1*(,*A % *)<",%;0-5*A +":; R – -.080,/ %:1$0;0,%9 +0,*$" 1 -%-.040 1*(,*--*$01*A )"-.1*) – %:*8)*8%$/,"9 +":", %.
D.080,/ %:1$0;0,%9 +0,*$" 1 -%-.040 1*(,*--*$01*A )"-.1*) – EFD )"--;%.71"$% 8* +*)42$0 R=100D/(D+r). G*-.*10),*-./ 8*$2- ;0,,7@ )0:2$/.".*1 8)*10)9$% 40.*(*4 «110(0,*-,"A(0,*» (."#$. 2).
!"#$%&" 2. |
|
H0:2$/.".7 *8)0(0$0,%9 +0,*$", 5)0:*$*1, !-$%&$- |
|
|||||||||
|
|
|
|
|
#2.%$+0,*$" % %*,*$" 1 %--$0(204*A 8)*#0 |
|
||||||
|
|
|
|
|
|
|
|
|
|
|
||
|
I10(0,*, 4</(43 |
J"A(0,*, 4</(43 |
|
±( |
|
Sr |
|
W,% |
||||
|
|
|
|
|
|
+0,*$ |
|
|
|
|
|
|
|
|
1,00 |
|
|
1,05 |
|
0,05 |
|
0,044 |
|
4,8 |
|
|
|
0,10 |
|
|
0,09 |
|
0,007 |
|
0,006 |
|
7,7 |
|
|
|
5)0:*$7 |
|
|
|
5)0:*$7 |
|
|
|
|||
!- |
|
"- |
|
#- |
|
|
|
|
|
|||
|
|
|
|
|
|
|
|
|
||||
1,00 |
|
1,00 |
|
1,00 |
3,07 |
|
0,07 |
|
0.061 |
|
2.3 |
|
0,10 |
|
0,10 |
|
0,10 |
0,29 |
|
0,008 |
|
0.007 |
|
2.8 |
|
|
|
|
|
|
|
!-$%&$-#2.%$+0,*$ |
|
|
|
|
||
|
|
1,00 |
|
|
0,98 |
|
0,04 |
|
0,035 |
|
4,1 |
|
|
|
0,10 |
|
|
0,11 |
|
0,006 |
|
0,005 |
|
5,5 |
|
|
|
|
|
|
|
%*,*$ |
|
|
|
|
|
|
|
|
1,00 |
|
|
0,97 |
|
0,03 |
|
0,026 |
|
3,1 |
|
|
|
0,10 |
|
|
0,09 |
|
0,004 |
|
0,004 |
|
4,5 |
|
224
!"#$%&'%(")$#$*((*+ ,-./-0%)#123* 0#4%) 56)7 &%3#0%(1#$*- (* 18+ *(*829* *()2#3"21*()#$ :%(#87(#;# )2<* 2 (29'2= :%(#8#$ <&2 2= "#$0%")(#0 <&2">)")$22 $ 0*"8#42$#? <>3@22.
!"#$%'%&
1.A#;2("32? ,.B. C%(#87(6% *()2#3"21*()6. D., 1988. 246 ".
2.A>1*3#$ E.F., G#(#0*&%$ B.H., G#8+("32? I.I., JK5*&7 B.,. .2&6. /202L%"32? "#")*$ 2 M3"<%&)29* 3*L%")$*. D., 2005. 212 ".
3.I#&%(0*( N.O., P&0#8*%$* Q.H., G#1#82(* P.B. // .>&(. *(*82). =2-
022. 1996. Q. 51. R5. S. 486–492.
()*+,(-(./0(1 2*3*4,2, 5674!52!(2/83(9 5.:/4.7 ;*:4,( <.4,:/.,/=( >2*:5651 ?:5)@:12!*++5A5
(.(. 6&BCD"C&1, A.A. 6&E&F"C&2, 2.A. 2&G"EH$I2, *.2. .&%JKI&2
1!"#$%&'(#)* +,-,%+.,* -/$,0"+.,* +&1 23 456, 7'. 8/'/$/1/',#+.&9, 101, 6/:/+,-,%+., 630090 (4/++,9) E-mail:bayandina@ngs.ru
26/:/+,-,%+.,* ,#+$,$7$ /%;,0"+./* <,=,,
,=. 6.6. >/%/?@/:& 23 456, A%. 5.&1"=,.& B&:%"#$(":&, 9, 6/:/+,-,%+., 630090 (4/++,9)
I&*"2)%82, <#8>L*%06% 29 &*")%(2?, 29$%")(6 " 1*$(2= <#& [1, 2]. T$%#? <&+$8%((6? (Hypericum perforatum L.) +$8+%)"+ #1(20 29 "*06= '2# <&20%(+$'2="+ 3&*"287(6= &*")%(2? 18+ #3&*"32 '%&- ")2, 87(* 2 =8#<3* [3, 4]. G&2 #3&*"3% &*")%(2+02 2()%("2$(%% #3&*'2- $*K)"+ 5%83#$6% $#8#3(* '%83* 2 '%&")2, "8*5%% – =8#<L*)#5>0*4(6% 2 87(+(6%.
H*02 <$#1282"7 #<6)6 <# #3&*'2$*(2K '%&")+(#? <&+42 M3"- )&*3)*02 9$%#+ <&+$8%((#;#. U8+ #3&*'2$*(2+ 2"<#879#$*8*"7 5%8*+ '%&")7, 3><8%((*+ > *8)*?@%$ (V#&(6? B8)*?, F%86? F#0) 2 9*- )%0 "<&+1%((*+ (*02 $&>L(>K. G&+4* "0#)*(* $ <*"06 <# 50 ;. W$%)>- X2% <#5%;2 9$%#+ <&+$8%((#;# "#&)* T#8#)#1#82("32?, $6&*-
225
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
#$ #& /+&1$* $%#" 30 *0#. C$/+" :2/)-&%0-$6> 2-&/0)"+> -&/)6$- 1(+ $)D0+.)-$6, /(-." /#$6& 9&+0)$ 6$4$;, 4$6"4"#$ 4$ 20'"#0> 0 6(4"-@$ #& /+&1$* $%#" 30 *0#, 3)$1( '$+730). 6)$-$; $)6&-. E)$- -$; $)6&- $)D0+.)-$6 0 /*"8 / '"-6(*, :)& /*"/. 0/'$+.9$6&+&/. 4+> $2-&/20 8"-/)0. =+> '-$)-&620 0/'$+.9$6&+0/. 1% -&/)6$-( '-$- )-&6, 4+> /$+"; @"+"9& 0/'$+.9$6&+0/. 0,1% -&/)6$-(. 5&*0 0/'$+.9$- 6&+0/. )-0 /'$/$1& '-$)-&6+06>.
C-"46&-0)"+.#&> '-$)-&62&. E -&/)6$- '-$)-&6( $'7/2&+0 30/)7? *$2-7? '->@7 0 6(4"-@06&+0 '-0 60 !F 6 )"3"#0" 30 *0#. G&)"* 6(- #0*&+0 '->@7 09 -&/)6$-&, 4&6&+0 /)"3. '-$)-&6", '"-"2+&4(6&+0 6 $)6&- /(-.> 0 6(4"-@06&+0 30 *0# '-0 90 !F.
H4#$6-"*"##&> '-$)-&62&. C-0%$)$6+"##(; -&/)6$- '-$)-&6( 6(+06&+0 6 $)6&- /(-.>, $'7/2&+0 6 #"%$ 30/)7? *$2-7? '->@7 0 6(- 4"-@06&+0 30 *0# '-0 60 !F.
C$/+"47?!&> '-$)-&62&. F#&3&+& 6(4"-@06&+0 '->@7 30 *0# '-0 60 !F 6 $)6&-" /(-.>, 9&)"* '"-"2+&4(6&+0 '->@7 6 '-0%$)$6+"##(; -&/)6$- '-$)-&6( 0 6(4"-@06&+0 30 *0# '-0 60 !F.
I"9 '-$)-&6( 8"-/). $2-&806&")/> 6$4#(* :2/)-&2)$* 96"-$1$> '-$4(->6+"##$%$ 6 /6")+$-2$-03#"6(; <6"). C-$)-&6( 0 /'$/$1 '-$- )-&6+06> 6+0>?) #& <6") $2-&8"##$; 8"-/)0 ()&1+. 1).
J&9+03#(" /'$/$1( '-$)-&6+06> $2&9(6&?) 6+0>#0" #& $))"-
#$2 <6")&. C-0 '$/+"47?!"; '-$)-&62" $))"#$2 '->@0 '$+73&")/> 1$- +"" )"*#(* 0 0#)"#/06#(*.
226
!"#$%&" 1. '()*" +),-*%, ./,"+)00.1 2(),.#.)3 4,.56,7($)0063 - ,"2063% 4,.*,"("3%
8,.*,"(" |
8,)5(",%*)$90"7 |
:50.(,)3)00"7 |
8.-$)5;<="7 |
|
4,.*,"(/" |
4,.*,"(/" |
4,.*,"(/" |
||
|
||||
>$<3./"$%)(6) |
8"$)(61 |
?)@)(61 |
A()*$.- |
|
/("-&6 |
|
|
/.,%B0)(61 |
|
A),0./%-$"7 3)59 |
C"/% |
:$%(/.(61 |
!)30.- |
|
|
|
|
/.,%B0)(61 |
|
A),0./%-$61 |
?)@)(61 |
?)@)(61 |
?)@)(61 |
|
3"D0%1 |
|
|
|
|
E(;F$.,%-*.) |
:,"0@)(.- |
G)$*61 |
!),,"/.*.(61 |
|
.$.(. |
/.,%B0)(61 |
|
|
|
A),0./%-$61 |
A()*$.- |
8"$)(61 |
?)@)(61 |
|
&%0/ |
/.,%B0)(61 |
|
|
|
?%F,.3"* /"$%7 |
|
|
!)30.- |
|
|
|
|
/.,%B0)(61 |
|
H%00./%-$"7 |
|
|
?)@)(61 |
|
3)59 |
|
|
|
|
A),0./%-$.) 2"- |
|
|
!)3061 F"/% |
|
/%-0.) @)$)2. |
|
|
|
I,"-7=%) ()=)-*(", 2"/,)4%(+%)-7 0" +),-*%, .4,)5)$7$%-9 3)- *.5.3 HJGC - 5%.50.-3"*,%B063 % 3"----)$)/*%(063 5)*)/*%,.("- 0%)3. E$7 HJGC-"0"$%2" /("2%-;0%(),-"$9063 3)*.5.3 K/-*,"/&%% 4,%,.506F /,"-%*)$)1 - ./,"+)00.1 4.5$.@/% 7($7)*-7 /%-$.*061 D%5,.$%2 [5]. E$7 F,.3"*.D,"L%B)-/.D. "0"$%2" 0"()-/; .#,"2&" (0,5– 3 3D) /%47*%$% 10 3%0 ( 300 !l ,-," HCl (37%):(.5":3)*"0.$ (2/1/1, v/v/v), ,"-*(., #6-*,. .F$"@5"$%, L%$9*,"* .*5)$7$% .* (.$./.0 - 4.- 3.=9< &)0*,%L;D%. C,.3"*.D,"L%B)-/%1 "0"$%2 4,.(.5%$-7 0" @%5- /.-*0.3 F,.3"*.D,"L) «Agilent 1100 Series LC/MSD». M"25)$)0%) 4,.- (.5%$.-9 0" /.$.0/) - .#,"=)00.1 L"2.1 «Zorbax RX-C18», 4.6"150 33 (0"4.$0%*)$9 - 5%"3)*,.3 B"-*%& 5 !3 % ,"23),.3 4., 80 N). M"25)$)0%) ()$.-9 4,% D,"5%)0*0.3 K$<%,.("0%% -3)-9< O):P % 2,3% ,"-*(.," 3;,"(9%0.1 /%-$.*6 ( (.5) (0"B"$. – 40% 3)*"0.$", - 6-.1 5. 15-.1 3%0;*6 $%0)10.) ;()$%B)0%) .#Q)30.1 5.$% 3)*"0.$" 5. 90%) 4,% -/.,.-*% 4.*./" 1 3$/3%0. I.$.0/" *),3.-*"*%,.("$"-9 4,% 30 #A.
!%4%B0"7 F,.3"*.D,"33" D%5,.$%2"*" (.$./.0, ./,"+)006F K/-- *,"/*.3 2(),.#.7, 4./"2"0" 0" ,%-;0/). C","/*),%-*%/% ()=)-*(, .#0"- ,;@)006F ( D%5,.$%2"*), 4,%()5)06 ( *"#$%&) 2.
227
!"#$%'"%$$% '()"#*(+%&% ,-."%/.( +,0"#1#0$, +%"0'(/&"("#,%22%3 2% 4#*#-
/0 440!40 2$
5%1*(6% 2. !"#$%'"%7(80/.(0 9%"%.&0"(/&(.( ,0:0/&,, #12%";<022-9 , '()"#*(+%&0 ,#*#.#2, #."%=022-9 +,0"#1#0$
|
A"0$3 |
>%1*?)%0$-@ 4/0,)#$#*0.;*3"2-@ (#2 |
Cmax, 2$ |
|
|
,-9#)%, |
[B+>]+ |
[B–>-] |
|
|
$(2 |
|
|
|
|
2,28 |
– |
– |
240, 278, 314, 362, 534 |
|
2,90 |
– |
– |
240, 280, 315, 358, 535 |
|
3,19 |
– |
303,1 |
261, 296 |
|
4,24 |
287,1 |
285,1 |
242, 276, 435, 528 |
|
10,50 |
303,1 |
301,1 |
256, 306, 372 |
D,0& =0"/&(, 4#."%=022#@ +,0"#1#0$, #4"0)0*30&/3 80&-"E$3
.#$4#202&%$( – / Rt 2.28, 2.90, 4.24 ( 10.50 (,0:0/&,# / Rt=3.19 10/-
6,0&2#). F;)3 4# 9%"%.&0"; /40.&"#, ( ,"0$02%$ ;)0"<(,%2(3, 40",-0 80&-"0 ,0:0/&,% #&2#/3&/3 . .*%//; &%2(2#, (/#0)(202(0 / Rt=4.24 ,/&"08%0&/3 , 9"#$%'"%$$%9 '()"#*(+%, ,-."%/#. '%**%$(, .#"#@
.";=(2-, );1%, #*E9(), ,0:0/&,# / Rt=10.50 – .,0"60&(2.
D,0& ,-."%/#. / 10/6,0&2-$( 4"#&"%,2-$( .%&(#2%$( (Al3+, Zn2+) #4"0)0*30&/3 /##&2#=02(0$ #/2#,2-9 ."%/3:(9 ,0:0/&, – .,0"60&(2%
228
! "#$%!&$&!' " Rt=4,24 (!&. )*$+,$-!& .+/"!- 0$+"-1 * '+.#-2$3-45 ,*$-, / *-#+#$ "#$%!&$&!$ "##67/$- *#3#.&8 .#+!9&$*4$ #--$&.!. :+!($+ +/*$ .#3!9$"-*# #6#!; .+/"!-$3$5 <+!%/$- 0$+"-! "*$-3#-
.#+!9&$*45 ,*$- (<+#-+/*/ %# .+/0$&!' "$+.!"34( ,!&.#(), 8*$3!- 9$&!$ %#3! .*$+,$-!&/ – #- 6$2$*#=# %# </3$*#=# (#%*+$($&&/' <+#- -+/*/ ! <#"3$%8>7/' <+#-+/*/ " "$+.!"34( ,!&.#(, #%*+$($&&/' <+#-+/*./ " /3>(#./3!$*4(! .*/",/(!). :+#-+/*/ "$+.!"3#5 ($%1> "+/?8 "##67/$- 0$+"-! ?$3$*/-45 #--$., .#-#+45 ?/-$( &/.3/%4- */$-"' &/ ,*$- .+/"!-$3'. :#@-#(8 *4.+/".! 0$+"-! <# ($%! <+! <+!- ($+ +/*( "##-$&!! #"*&4; .+/"'7!; .#(<#&$&-#* !($>- ?$3$*/-4$ ,*$-/ (<+$%*/+!-$31&/' ! #%*+$($&&/' <+#-+/*./). )+#($ -#=#, * *4.+/"./; <# ($% <+#-+/*$ 8*$3!9!*/$-"' %#3' %+8-
=!; .#(<#&$&-#*, !($>7!; <#=3#7$&!$ " Amax.~ 534 &(.
B/.!( #6+/?#(, ,*$- 0$+"-!, *4.+/0$& @."-+/.-#( ?*$+#6#', #<+$%$3'$-"' "##-$&!$( .*$+,$-!&/ ! "#$%!&$&!' " Rt=4,24. C!<$- +!,!*4$ .#(<#&$&-4 &$ 643! #6&/+82$&4 * =!%+#3!?/-/;, *$+#'-, #&! &$ D!."!+8>-"' &/ *#3#.&/; 0$+"-!.
!"#$%'%&
1.)#+#3>. E.F. G"-#+!' !?89$&!' .+/"!31&4; +/"-$&!5 * H#""!! / :#- 3#"1(/. I.J., K/+.#*/ L.L. )#"->( ! -$."-!31 </?4+4.,$* F3-/' (IVIII **. %# &.@.). I#*#"!6!+"., 2005. M. 202–205.
2.)#+#3>. E.F. )+/"!31&4$ +/"-$&!' M!6!+! / B$."-!31 !? «?/($+?- 0!;» (#=!3 C#+=# F3-/' IV-III **. %# &.@. (#<4- ($2%!",!<3!&/+- =# !""3$%#*/&!') /I.J. :#3#"1(/. ! %+.; #-*. +$%. K.F. L!-*!&".!5; H#"././%. &/8.; G&-- /+;$#3#=!! ! @-=+/D!! [! %+.]. I#*#"!6!+"., 2006. M. 133–199.
3.)#+#3>. E.F. )+/"!31&4$ +/"-$&!' F3-/' ! "#<+$%$31&4; -$++!-#- +!5 // N!(!' +/"-!-$31=# "4+1', 2003. O1, M. 101–135.
4.)/"8(#* P.F. I$.#-#+4$ %!.#+/"-87!$ .+/"!31&4$ +/"-$&!' F?$+- 6/5%2/&/ ! <$+"<$.-!*4 !; !"<#31?#*/&!' * &/+#%( ;#?'5"-*$ //
H/"-!-. +$"8+"4. 1971. B. 7. J4<. 4. M. 539–547.
5.Wouters J. High-performance liquid chromatography anthraquinones: analysis of plant and insect extracts and dyed textiles // Studies in conservation. 1985.V. 30. P. 119–128.
229