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First massage the anal opening with the front of the anoscope
e
5 Common Methods ofExamination forAnal Fistula
55
The direction of the lens should be facing the navel rst and then facing the sacral tail after passing through the anal canal. After reaching the ampulla of rectum, the core should be pulled out to observe whether there is adhesive mucus or blood on the top of the core. After adjusting the light to directly illuminate the visual eld in the tube, carefully observe the color of the mucosa at the lower end of the rectum, whether there are tumors, polyps, ulcers, foreign bodies, secretions, etc. Then, slowly withdraw the mirror body from the anus, and observe whether there are internal hemorrhoids, anal nipple hypertrophy, anal sinus­itis, or anal stula opening near the dentate line. Below the dentate line, look for cracks, growths, etc. (Fig. 5.11). In order to observe the lesion
Remove the anoscope core
Fig. 5.11 Anoscopy method
Insert the anoscop towards the umbilicus
carefully, it is sometimes necessary to insert the lens several times. Note: during the examination, if further observation is required or the rotating mirror body is required, the core must be inserted again before operation to prevent injury to the anal canal and rectal mucosa.
In patients with anal stula, there is often con­gestion and swelling in the internal opening in the dentate line, or there are red, inamed pits and protruding nodules. Because the anal canal is dilated, the stula wall is compressed; some­times, pus can be seen owing from the internal opening to the intestinal cavity. At this time, if methylene blue is injected from the external opening of stula, the overow of methylene blue from the internal opening can be seen, or the gauze placed in the anus will have methylene blue.
Leaf Anoscope
Before anoscopy, ask the patient to empty his stool. The surgeon should hold the handle of the anoscope in his left hand, close all the leaves of the leaf anoscope, smear parafn oil on them, insert them into the anus, and then open them up. The lesion is observed by using the space between the leaves (Fig.5.14). Note: during the examina­tion, do not suddenly pull up the leaf anoscope to prevent the injury of intestinal mucosa and do not force to push in order to prevent stabbing the intestinal wall.
5.1.2.5 Inspection ofAnal Crypt Hook
Crypt hook examination is an important method to examine the internal opening. There are two kinds of hooks commonly used: the hooks are
0.5cm and 1.0 cm, respectively. When using the crypt hook probe, rst take the small hook, probe the suspicious lesion area of the anus under the microscope, and then slowly explore the rest of the anal stula along the dentate line. If neces­sary, take the hook to explore again. In general, the normal crypt can be probed by the crypt hook, but the hook is shallow. If the hook is deep, the location of the internal opening should be highly suspected; if the direction of the hook is consis­tent with the direction of the anal stula, the loca­tion of the internal opening can be claried. The
56
Fig. 5.12 Staining examination of anal stula
lower stula is then inserted with a probe from the external opening. If the two meet and touch, it indicates that the explored internal opening and stula are interlinked, and the anal crypt is where the internal opening is.
5.1.2.6 Methylene Blue Staining Examination
The staining agent is injected into the stula from the external opening of the anal stula to stain the wall of the stula so as to show the position of the internal opening and determine the range, course, morphology, and number of stula (Fig.5.12). The commonly used staining agent is 2% methy­lene blue or 2% methylene blue mixed with 1% hydrogen peroxide. Specic examination meth­ods are as follows:
Fill theGauze Roll intheAnus
Take the anus mirror and apply lubricant and insert it into the anus, extract the lens core, and then put the rolled yarn roll into the anus, and then slowly take out the anus mirror so that the gauze roll is placed in the anal canal. During the operation under anesthesia, the gauze can be directly put into the anus.
Dye Injection
Use a 5-ml syringe to extract 2–4ml of methy­lene blue, cut off the needle of the scalp needle hose, connect the syringe to the scalp needle hose, insert the hose through the external opening into the stula for more than 1–2 cm, and press
R. Shi and L. Liu
on the external opening to prevent methylene blue from spilling out of the external opening. Inject methylene blue slowly. After infusion, con­tinue to press the external opening to prevent methylene blue from spilling out, and then lightly press and knead the stula for observation.
Observation ofStaining Area
The observation of the internal opening staining area can be divided into direct observation and indirect observation. At the same time as inject­ing the liquid, direct observation is made by opening the anus and looking directly at the col­ored spot, while indirect observation is made by identifying the colored area of the yarn coil. When the anal plug gauze rolls out, rst observe whether there is any color or not. If there is stain­ing, it indicates that the internal and external opening of stula is on the same path. The posi­tion of the internal opening can be determined by observing the position of the shading area, and the corresponding area of the shading area is the position of the internal opening. However, when the internal opening is large, the stain will over­ow more, and it is difcult to accurately identify the location of the internal opening. In the case of internal opening atresia, although no staining agent is spilled from the anus, sometimes blue dye can be seen in the anal mucosa, so the loca­tion of the internal opening can also be determined.
In addition, the anoscope can also not be removed during the staining examination to observe whether the dye spills from the internal opening. This method is often used for intraop­erative examination of the internal opening.
5.2 Auxiliary Examination
ofFistula

5.2.1 Ultrasonic Examination

Ultrasound examination is a method to diagnose diseases by utilizing the information generated by the interaction between the physical charac­teristics of ultrasound and the acoustic character­istics of human organs and tissues and by
l
anal sphincter
5 Common Methods ofExamination forAnal Fistula
57
receiving, amplifying, and processing the infor­mation to form graphics, curves, or other data. Because it is painless, inexpensive, and portable equipment, it is widely used in the clinic.
5.2.1.1 Equipment
There are two types of transanal ultrasound: lin­ear and radial. The radial examination function can observe the pathological changes of the anus within a range of 360°, and the whole perianal image can be better displayed by using the ure­thra as the 12-point position (Fig.5.13). However, it is difcult to understand the longitudinal infor­mation of anal canal. If the inspector is familiar with the normal anatomic structure of anus dur­ing ultrasonic examination, he or she can make up for this deciency. During the examination, the ultrasonic probe inserted into the anus is moved up and down for several times to observe the anatomical relationship between the location of the primary lesion, the course of stula, and the anal sphincter and anal levator ani muscles surrounding the stula.
5.2.1.2 Examination Methods
According to the method of ultrasound echo dis­play, there are two methods. (1) The two­dimensional section diagnostic method (abbreviated as B-mode ultrasound) is the most widely used ultrasound diagnostic method in
clinics at present. In the anorectal department, it is often used for abdominal and perianal exami­nations. The real-time two-dimensional ultra­sound tomography of human soft tissue organs is obtained by linear scanning. (2) Color Doppler ow imaging (CDFI) is a method of using auto­correlation technology on a two-dimensional ultrasound section to quickly obtain all echo information in a larger chamber or pipeline and then color coding and overlapping in the corre­sponding area of the same two-dimensional gray­scale image. This can reect the blood ow movement situation inlocal tissues. According to the path of examination, it can be divided into the body surface ultrasonography and the endolumi­nal ultrasound. Anal stula is usually examined by endoluminal ultrasound. Because transanal ultrasound examination is specialized, it has great clinical signicance for anal stula and other diseases. This chapter mainly introduces the transanal ultrasound examination method.
Transanal ultrasonography
The enema should be given with 100ml of glyc­erol in advance, and the left lateral position should be taken. The ultrasonic probe should be inserted through the anus, and the urethra should be rstly scanned to mark the position of 12 o’clock. Then move the ultrasonic probe up and down to conrm the internal sphincter, external
Fig. 5.13 Image of radiant ultrasound system
Superficial external anal sphincter
Deep externa
58
R. Shi and L. Liu
sphincter, and levator ani muscles as diagnostic targets and observe the presence of lesions and the direction of expansion. In order to locate the dentate line, a crypt hook can be placed in the dentate line when combined with high inter­sphincteric lesions.
When the ultrasound probe is inserted from the anus, the lower part of the external sphincter is located at the supercial part of the lower part of the anus and shows low echo and round around the anus. The sphincter muscle bundle is weaker in females than in males, especially in the front of the anus. According to the contraction of the anus, the voluntary contraction of the muscle can be observed, and this indicates that the muscle bundle is striated muscle.
The supercial part of the external sphincter and the subcutaneous part of the circular external sphincter have obvious shape differences and show low echo. The supercial part of the exter­nal sphincter is the largest, longest, and strongest muscle bundle in the external sphincter. It shows three forms on ultrasound, but all three of them x the anus in the back from the left or right sphincters. When contracting the anus, random contraction of the muscle is observed, indicating that the muscle bundle is striated muscle. On the inner side of the supercial external sphincter, we can see that the deep external sphincter, which is described as a low echo, is circular around the deep anal sphincter as the subcutaneous part of the external sphincter. However, this muscle bun­dle is anatomically tightly attached to the poste­rior puborectalis muscle, and in many patients, it cannot be clearly scanned.
The levator ani muscle can be divided into three parts: puborectalis muscle, pubococcygeal muscle, and iliac coccygeal muscle according to its course. However, there is no clear demarca­tion between the three parts, so it is difcult to completely differentiate them in the ultrasono­graphic images. Because the internal sphincter is thin, in many patients it cannot be clearly described.
Endoscopic Ultrasonography
Endoscopic ultrasonography is simple, rapid, and easily accepted by patients. During operation, the
patient takes the left lateral position or the prone position, the probe is gently inserted into the dis­tal rectum, and the lens is retreated outward while the examination is performed.
There are three criteria for nding the internal opening under endoscopic ultrasonography: ① the internal sphincter is detected through the intersphincteric stula (positive predictive value: 80%); ② obvious defect of internal sphincter (positive predictive value: 79%); and ③ clear sub­epithelial stula with localized sphincter defect (positive predictive value: 94%). The overall sen­sitivity of these three signs in combination is 94% (specicity: 87%, positive predictive value: 81%).
5.2.1.3 Normal Anatomy ofTransanal
Ultrasound Examination
The images of internal sphincter, external sphinc­ter, and levator ani muscle are recorded by preop­erative ultrasonography. The muscles are exposed during the operation. Ultrasound examination is to be performed. If it is consistent with the preop­erative ndings, the muscles are identied as cer­tain muscles. In addition, cases of internal hemorrhoids and anal ssure are also examined by ultrasound. Through the above efforts, the normal ultrasound anatomy of anal canal can be mastered.
1. Internal sphincter
On ultrasound, it appears to be the inner­most region, circular around the anus, and appears to be hypoechoic. Due to the thin layer, many cases cannot be scanned.
2. The subcutaneous part of the external sphincter
Close to the outside of the internal sphinc­ter, a clear circular hypoechoic image is easily found (Fig.5.14).
3. Supercial external sphincter When the ultrasonic probe is moved
upward from the trace to the subcutaneous part of the external sphincter, the image is the supercial part of the external sphincter, and its shape is signicantly different from that of the subcutaneous part of the external sphinc­ter (Fig.5.15). The supercial appearance of
Internal anal sphincter
l
anal sphincter
5 Common Methods ofExamination forAnal Fistula
Fig. 5.14 Ultrasonic images of the subcutaneous and internal sphincter of the external sphincter
Fig. 5.15 Ultrasound images of the supercial and deep parts of the external anal sphincter
59
External Anal Sphincter
the external sphincter in the vast majority of cases is described as any of the three patterns shown in Fig.5.16, a distinct low echo.
4. Deep external sphincter At the same height as the supercial part of
the external sphincter, the inner part of the supercial part of the external sphincter is round around the anus and also presents as hypoechoic, but the deep image of the exter­nal sphincter in many cases is not clear.
5. Levator ani muscle At the height of the probe, it should be
scanned from the superficial and deep parts of the external sphincter; moving the probe upward, there is a low muscle echo image that is U-shaped around the anus (Fig.5.17).
6. Dentate line Under normal conditions, it is impossible
to locate the dentate line by ultrasonic exami­nation. Therefore, a liquid should be used to better characterize the characteristics of ultrasonic wave in the dentate line, i.e., with
0.5% serucaine E (0.5 ml) for local injection of about 5 places. This can accurately deter­mine the position of the dentate line. It is also possible to position the dentate line by placing a crypt hook at the dentate line portion.
5.2.1.4 Ultrasonic Characteristics
When meeting specic cases in the clinic, doc­tors should compare the results of palpation
Superficial external anal sphincter
Deep externa
ofDierent Types ofPerianal Abscesses andAnal Fistulas
60
a
b
c
Subcutaneous external
Urethra
Fig. 5.16 Three forms of the supercial part of the external sphincter. The three pictures A/B/C on the left show the ultrasound images of the supercial part of the external sphincter muscle traced at different positions A/B/C in the right picture
R. Shi and L. Liu
Puborectalis Muscle Deep external anal sphincter
a
b
Coccyx
c
Internal anal sphincter
Fig. 5.17 Ultrasonic image of the levator ani muscle
Superficial external anal sphincter
anal sphincter
Postanal Space
Levator Ani M.
with the ultrasound images and the intraopera­tive ndings so as to determine the ultrasound images of various types of perianal abscess and anal stula and grasp the characteristics of the lesions.
Intersphincteric Abscess, Intersphincteric Fistula (II Type)
On the ultrasonographic images, the intersphinc­teric lesions present as a hypoechoic mass in the medial external sphincter. The abscess is cystic,
External Anal Sphincter
External Anal
5 Common Methods ofExamination forAnal Fistula
61
and the anal stula is a mass with approximately the same echo density as the sphincter. The high intersphincteric stula (II H) or low intersphinc­teric stula (II L) can be distinguished by locally injecting serucaine E into the dentate line to locate the dentate line.
Case 1: II HA, II LA (Figs.5.18 and 5.19) In Fig. 5.18, the supercial external sphincter appears as a U-shaped hypoechoic layer sur­rounding the anus. The intersphincteric lesion presents as a 6-point hypoechoic mass. Figure5.19 is the image of the ultrasonic probe
Fig. 5.18 Ultrasound image of high intermuscular abscess
being pulled outward. The subcutaneous part of the external sphincter appears as a round hypoechoic layer around the anus. II LA and II HA also show the sphincter within 6-point inside the low echo. Therefore, a diagnosis of II HA II LA is made.
Case 2: II Hs (Fig.5.20), II Ls (Fig.5.21) The circular hypoechoic layer surrounding the anus in Fig. 5.20 is the supercial part of the external sphincter. At six o’clock, the masses at the same level as the supercial part of the exter­nal sphincter can be seen, showing as II Hs. In
Fig. 5.19 Ultrasound image of low intermuscular abscess
IIHA
(Superficial)
IILA
Sphincter (Superficial)
62
External Anal Sphincter (Subcutaneous)
Fig. 5.20 Ultrasound image of high intermuscular stula
R. Shi and L. Liu
Fig. 5.21 Ultrasound image of low intermuscular stula
External Anal Sphincter (Superficial)
IILs
IIHs
Fig. 5.21, the subcutaneous part of the circular external sphincter can be seen, and the lesion of II Ls can be seen at six o’clock. According to the above characteristics, the case is diagnosed as II Hs, II Ls.
Ischioanal Abscess, Ischioanal Fistula (Type III)
Because the gap behind the anus is full of pus, the ischioanal abscess shows a cystic shape in the gap behind the anus on ultrasonic examination,
and the pus is discharged simultaneously when pressing on the supercial part of the external sphincter. Ischioanal abscess needs to be differ­entiated from intersphincteric abscess. It is very difcult to identify by palpation, but it can be easily identied by ultrasound.
In the case of ischioanal stula, the purulent uid in the posterior anal space decreases, and the inner cavity becomes smaller, presenting as a low-echo mass extending along the supercial part of the external sphincter to the horseshoe-
IIHA
(Superficial)
5 Common Methods ofExamination forAnal Fistula
63
shaped masses on both sides. Most cases of ischiorectal space lesions are associated with intersphincteric lesions (mostly II H).
When Takano Masahiro studied the formation pattern of ischioanal abscess by ultrasonic examination, he found something very interest­ing: in the production process of ischioanal abscess, an intersphincteric abscess was formed rst, and then the intersphincteric abscess bursts through the external sphincter and extends deep into the posterior anal space, and then forms the ischioanal rectal abscess. When ultrasound exam­ines the posterior anal space, there is no posterior anal space near the outside of the internal sphinc­ter at the supercial height of the external sphinc­ter. On the outside of the internal sphincter, there is a deep part of the external sphincter, and the outside of the deep part of the external sphincter has a deep postanal space. Therefore, the infection of the anal gland between the internal and exter­nal sphincter does not directly spread to the deep space but rst forms the intersphincteric abscess. In some cases, the intersphincteric abscess rup­tures the deep external sphincter and spreads to the deep space, forming ischiorectal abscess and ischiorectal stula (Case 3).
When observing the lesions of the ischioanal abscesses with ultrasonic examination, the lesions in the deep space were rst seen, the
abscess is presented as a cystic shape, while the anal stula presented as a mixed echo mass. Almost all cases are accompanied by low echo masses (intersphincteric lesions) on the inside of the external sphincter, and it is necessary to prop­erly treat the lesions here during surgery (Case 3, Case 4).
Case 3: III BA (Fig.5.22), II HA, II LA (Fig.5.23) In Fig.5.22, II HA can be seen at six o’clock, and deep external sphincter can be seen behind it. The deep part of the external sphincter ruptured at six o’clock, and the lesion spread to the posterior anal space. In Fig.5.23, we can see the subcuta­neous part of the sphincter round around the anus, in which II LA can be seen. According to the above ndings, the diagnosis of this case is III BA, II HA, and II LA.
Case 4: III Bs, II Hs (Fig.5.24) Unlike in the abscess stage, in the posterior anal space at 6 points, brin in the pus precipitates after a long period of development and becomes a horseshoe-shaped lesion with a mixture of strong echo and low echo, and is expressed as III Bs. The hypoechoic mass, II Hs, can be seen in the inner side of the internal sphincter at 6 points. Therefore, the disease is diagnosed as III Bs, II Hs.
Fig. 5.22 Ultrasonic images of ischiorectal fossa abscess and high intermuscular abscess
External Anal Sphincter
(Deep) External Anal Sphincter
Postanal Space
III BA
64
Fig. 5.23 Ultrasonic images of pus species and low intermuscular abscess in ischiorectal fossa
Fig. 5.24 Ultrasonic images of ischiorectal fossa stula and high intermuscular stula
External Anal Sphincter (Subcutaneous)
R. Shi and L. Liu
IILA
IIIBA
Pelvirectal Abscess, Pelvirectal Fistula (Type IV)
As with the ischiac rectum lesion, except for the result of Crohn’s disease and foreign body, it is rare for individuals to have multiple intersphinc­teric lesions and ischiac rectum lesions.
IIHs
External Anal Sphincter (Deep)
IIIBs
It is believed that it is caused by the upward spread of the high intersphincteric lesions (II H) or the upward spread of the ulceration of levator ani muscle in ischiorectal fossa lesions (III). Therefore, pelvic rectal lesions are mostly associated with intersphincteric lesions and