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Файл:Ординатура / Хирургия / Библиотека им академика М.И. Перельмана / Книга_3777_Библиотеки_им_академика_М_И_Перельмана
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CHAPTER 5 Collection by Routine Venipuncture
D
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G
A
B
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Figure 5-4 Venipuncture with the butter y needle system. (A) Attach butter y system to syringe. (B) Apply
tourniquet with patient’s hand closed. (C) Prep selected site with alcohol. (D) Insert needle at approximately
5–10 degree angle. (E) Slowly pull blood into syringe. (F) When the appropriate amount of blood has lled the
syringe, remove tourniquet withdraw needle and apply pressure to site. (G) Immediately activate safety feature on
needle. (H) Dispose of needle into sharps container. (I) Check to ensure bleeding has stopped. Apply bandage.
Copyright 2010 Cengage Learning. All Rights Reserved. May not be copied, scanned, or duplicated, in whole or in part. Due to electronic rights, some third party content may be suppressed from the eBook and/or eChapter(s).
Editorial review has deemed that any suppressed content does not materially affect the overall learning experience. Cengage Learning reserves the right to remove additional content at any time if subsequent rights restrictions require it.
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PART 2 Blood and Urine Collection
21. Attach a blood transfer device.
22. Transfer blood into appropriate collection tubes using correct
order of draw. The order of draw for a syringe is the same as for
the evacuated system. Do not remove the stopper from the collection tube and manually ll the tube. Removing the stopper may
allow for under lling or over lling the tube. In addition, there is
a greater risk to exposure of blood should there be a spill. Do not
leave the needle on the syringe and puncture the stopper. This increases the risk of an accidental needlestick to the phlebotomist.
23. Allow vacuum to draw blood into tube. Do not forcefully depress
the syringe plunger.
24. Gently invert tubes 4-5 times to mix anticoagulant with blood.
25. Discard needle and transfer device into sharps container.
26. Label tubes with appropriate information, and bag for transport.
27. Bandage patient.
28. Discard gloves.
29. Clean up area.
30. Thank patient.
31. Wash hands.
Please refer to Figure 5-4.
Copyright 2010 Cengage Learning. All Rights Reserved. May not be copied, scanned, or duplicated, in whole or in part. Due to electronic rights, some third party content may be suppressed from the eBook and/or eChapter(s).
Editorial review has deemed that any suppressed content does not materially affect the overall learning experience. Cengage Learning reserves the right to remove additional content at any time if subsequent rights restrictions require it.
SUMMARY
Careful blood collection techniques are critical for the phlebotomist.
Organizing the workload, preparing the patient for the venipuncture in a
pleasant manner, assembling the equipment ef ciently, and performing a
smooth, successful venipuncture are crucial aspects of the phlebotomist’s
work. Taking care not to injure the patient should also be foremost in the
phlebotomist’s mind, as well as ensuring personal safety during the blood
draw. Experience will provide the phlebotomist with a smooth, ef cient
technique, both for communicating well with the patient and for performing a successful venipuncture.
REVIEW ACTIVITIES
1. Absolute identi cation of the patient is mandatory to __________
______________________________________________________.
2. ASAP orders have priority over
orders.

CHAPTER 5 Collection by Routine Venipuncture
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3. The hospital phlebotomist should awaken a patient before
collecting a blood sample because
.
4. The phlebotomist should not draw specimens above an intravenous
site because
.
5. A blood sample should not be collected from the arm on the side
of a mastectomy because
.
6. Specimens should not be collected from a hematoma area
because
.
7. The evacuated collection system should not be used on a small,
fragile vein because
.
8. Blood cultures should always be drawn rst when drawing
multiple tubes because
.
115
9. Blood bank specimens should be drawn in
tubes rather than in gel-separator tubes.
10. When performing a venipuncture, the most frequently used
veins in the forearm are the
11. A good, healthy vein feels
patient’s arm for longer than
and the .
to the touch.
minute.
DISCUSSION QUESTIONS
1. You need to collect a CBC, protime, amylase, and BUN on a
patient. After assessing the patient’s veins, you determine that
the best vein is on the right hand. What are the steps involved in
collecting the blood specimen from this patient?
2. You enter the inpatient room of Mr. Smith. You nd that he has
no armband. However, you know Mr
your high school science teacher. You are going to collect a CBC
and B12. He has an IV in his right hand. What are the steps
involved in collecting the blood specimen from Mr. Smith?
. Smith, because he was
-stopper
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Editorial review has deemed that any suppressed content does not materially affect the overall learning experience. Cengage Learning reserves the right to remove additional content at any time if subsequent rights restrictions require it.

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“The differences in venous
and skin-puncture blood
should not restrict the use
of skin-puncture blood.”
—National Committee
for Clinical Laboratory
Standards (1986)
Collection by Skin
Puncture
After studying this unit, it is the responsibility of the learner to be able to:
1. List several situations in which the preferred method of blood collection
is skin puncture.
2. Explain several situations in which skin puncture is not the preferred
method of blood collection.
3. Describe the appropriate site selection criteria for heelsticks and
ngersticks.
4. State the steps in performing a ngerstick.
5. Demonstrate the appropriate steps in performing a ngerstick.
6. State the steps to performing a heelstick.
Copyright 2010 Cengage Learning. All Rights Reserved. May not be copied, scanned, or duplicated, in whole or in part. Due to electronic rights, some third party content may be suppressed from the eBook and/or eChapter(s).
Editorial review has deemed that any suppressed content does not materially affect the overall learning experience. Cengage Learning reserves the right to remove additional content at any time if subsequent rights restrictions require it.

capillary action the process by which blood
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automatically ows into a thin tube during a
capillary blood collection procedure
cyanotic displaying blueness of the skin, as from
imperfectly oxygenated blood
interstitial uid uid between the tissues
lateral referring to the side
osteomyelitis an in ammatory disease of the
bone, resulting from infection
plantar relating to the sole of the foot
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S
PART 2 Blood and Urine Collection
kin puncture, or capillary puncture, is a procedure in which
the skin is punctured with a lancet to obtain a capillary blood
sample for laboratory testing. While laboratory tests are most
often performed on venous blood, there are situations in which
venipuncture is not appropriate.
CHOOSING THE SKIN PUNCTURE
In several situations, the skin puncture may be preferable to the venipuncture.
These situations may arise when:
The patient is an infant, and a good vein is not found.•
The patient is a small child, and either a proper vein is not found •
or the child will not cooperate with the venipuncture.
Tests are ordered that require only a few drops of blood.
•
The patient is very apprehensive about a venipuncture and insists •
on a skin puncture (parents can also request this procedure for
their child).
Repeated venipunctures are not successful.•
The patient has severe burns.•
The patient has thrombotic tendencies.•
The patient’s veins must be reserved for therapeutic purposes such •
as intravenous lines.
The patient has fragile super cial veins.•
A skin puncture is not appropriate when:
The patient is severely dehydrated. •
The patient is in shock.•
The patient has chronic poor circulation.•
The patient is extremely cold.•
More blood than can be obtained by a skin puncture is needed for •
testing.
Copyright 2010 Cengage Learning. All Rights Reserved. May not be copied, scanned, or duplicated, in whole or in part. Due to electronic rights, some third party content may be suppressed from the eBook and/or eChapter(s).
Editorial review has deemed that any suppressed content does not materially affect the overall learning experience. Cengage Learning reserves the right to remove additional content at any time if subsequent rights restrictions require it.

CHAPTER 6 Collection by Skin Puncture
This
Not this
B
Ring
Great
A
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COMPOSITION OF SKIN PUNCTURE BLOOD
Skin puncture blood is different in composition from venous blood. Blood
obtained by skin puncture is a combination of blood from capillaries, arterioles, and venules. It more closely resembles arterial blood than venous
blood. Skin puncture blood may be used for laboratory testing so long as
the technique is taken into consideration when interpreting the results of
blood tests and when a small sample of blood is suf cient for the tests.
As the skin is punctured, tissue is damaged and
interstitial uid—
the uid present between tissues—is released. The rst drop of blood will
be diluted by this and uid will need to be wiped away.
SITE SELECTION
A skin puncture (also called a capillary puncture) may also be performed
on ngertips, heels, toes, or earlobes. However, the earlobe is not recommended as a site of choice, due to poor capillary access.
Children and Adults
Generally, the site of choice is the portion of the fingertip shown in
Figure 6-1. Do not puncture the side or the tip of the nger. The ring
nger and the middle nger are the appropriate ngers for puncture.
Delmar/Cengage Learning
Figure 6-1 Finger puncture site.
Delmar/Cengage Learning
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PART 2 Blood and Urine Collection
The thumb, rst nger, and fth nger are not appropriate choices for
skin puncture. The tissue on the fth nger is much thinner than on
the other ngers. The thumb and rst nger are usually calloused. The
nondonimant hand may be a better rst option since the ngers may be
less calloused. Patients requiring frequent ngersticks—such as for glucose monitoring, for example—should have the puncture site rotated.
The puncture should be made perpendicular to the ngerprint. A perpendicular puncture will cause the blood to form a droplet. A parallel
puncture will cause the blood to ow down the nger instead of forming
a nice round drop.
Edematous (swollen) sites should be avoided, because a free ow of
blood is impossible. Punctures in cold, or
cyanotic, areas should also be
avoided. Blood obtained from these sites may result in falsely high hemoglobin or cell-count values.
Infants
The site of choice is the lateral (side) area of the plantar surface of
the heel (“plantar” refers to the sole of the foot). The darkened areas
in Figure 6-2 show the preferred areas in which to perform a heelstick. Never use the area of the heel bone as a puncture site. In addition, never puncture the area of the arch. Puncturing either of these
areas may have serious consequences for the infant. Puncturing the
heel bone may result in infection and
disease of the bone. Puncturing the arch area may cause damage to
nerves. Never perform a puncture that will go through a previous site.
Never perform a skin puncture on the nger of an infant. The tissue
on an infant’s nger is much too thin, and the procedure could damage
the bone.
osteomyelitis, an in ammatory
Copyright 2010 Cengage Learning. All Rights Reserved. May not be copied, scanned, or duplicated, in whole or in part. Due to electronic rights, some third party content may be suppressed from the eBook and/or eChapter(s).
Editorial review has deemed that any suppressed content does not materially affect the overall learning experience. Cengage Learning reserves the right to remove additional content at any time if subsequent rights restrictions require it.
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Figure 6-2 Heel puncture site.

CHAPTER 6 Collection by Skin Puncture
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121
PREPARING THE SITE
A free ow of blood is important for accurate test results. Massaging the
nger of the adult or child and the heel of the infant until the skin is pink
will stimulate circulation. Warming the skin will also increase blood ow.
A wet towel (or a packaged heating product) comfortably warmed to the
touch may be applied to an adult’s or child’s nger or to the heel of an
infant. The heat source should be applied for at least 3 minutes.
COLLECTION DEVICES
A wide variety of devices are now used to collect, process, and transport
blood obtained from a skin puncture. Traditionally, blood is obtained with
disposable capillary pipettes that resemble plastic straws. The blood ows
into the tube through
The process of collecting skin puncture blood has been simpli ed by
the introduction of different types of microcollection devices. Figure 6-3
shows two such devices. These collection devices allow for easier measuring,
color coding, stoppering, centrifugation, and storage of the blood samples.
Collection caps, often shaped like a scoop, are used to collect the blood.
capillary action.
Copyright 2010 Cengage Learning. All Rights Reserved. May not be copied, scanned, or duplicated, in whole or in part. Due to electronic rights, some third party content may be suppressed from the eBook and/or eChapter(s).
Editorial review has deemed that any suppressed content does not materially affect the overall learning experience. Cengage Learning reserves the right to remove additional content at any time if subsequent rights restrictions require it.
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Figure 6-3 Microcollection devices.

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PART 2 Blood and Urine Collection
Lancets
A good ow of blood is obtained with the correct use of the skin puncture
device. As with the collection devices, a wide variety of skin puncture lancets are available.
Lancets are designed to control the depth of the puncture so that no
damage will be done to the site. Most lancets are either blades that the
phlebotomist pushes into the skin or spring-loaded devices that lie on the
surface of the skin and make an automatic puncture. The spring-loaded
devices have a button that, when pressed, releases the lancet into the skin.
Patients may be less frightened by this device, because they cannot see
the actual blade penetrate the skin.
1. Identify and greet the patient. You must always be sure that the
specimen is obtained from the correct patient.
2. Explain the procedure. The explanation should help to calm the
patient and instill con dence in your ability as a phlebotomist.
3. Assemble the equipment: gloves, lancet or puncture device,
alcohol prep pads, gauze, and collection device.
4. Select a puncture site.
5. Massage or warm the puncture site. The heel may be warmed
with a warm cloth not to exceed 42ºC (107ºF). Commercial
infant heel warmers are also available.
6. Put on gloves to prevent exposure to bloodborne pathogens.
7. Cleanse the ngertip with the alcohol prep pad to prevent
microbiological contamination of the patient and the specimen.
8. Dry the ngertip to prevent hemolysis of the specimen from expo-
sure to alcohol, and to reduce a stinging sensation for the patient.
9. Remove cover from lancet or other device to be used.
10. Grasp patient’s nger, placing your thumb at the base of the
ngernail. Wrap your ngers around the inside of the patient’s
nger. This maintains your control of the patient’s nger while
allowing you the best access to the puncture site.
11. If using a lancet, make a quick down-and-up motion with the
lancet to rmly puncture the skin. One rm and quick puncture
is less painful than a repeated puncture because of inadequate
Copyright 2010 Cengage Learning. All Rights Reserved. May not be copied, scanned, or duplicated, in whole or in part. Due to electronic rights, some third party content may be suppressed from the eBook and/or eChapter(s).
Editorial review has deemed that any suppressed content does not materially affect the overall learning experience. Cengage Learning reserves the right to remove additional content at any time if subsequent rights restrictions require it.
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