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Файл:Ординатура / Хирургия / Библиотека им академика М.И. Перельмана / Книга_3777_Библиотеки_им_академика_М_И_Перельмана
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CHAPTER 4 Blood Collection Equipment
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93
Delmar/Cengage Learning
Figure 4-11 Microcollection tubes. See color insert.
Figure 4-12 Unopette blood diluting unit. See color insert.
Delmar/Cengage Learning
Copyright 2010 Cengage Learning. All Rights Reserved. May not be copied, scanned, or duplicated, in whole or in part. Due to electronic rights, some third party content may be suppressed from the eBook and/or eChapter(s).
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PART 2 Blood and Urine Collection
SUMMARY
One of the most important tasks for the phlebotomist is to learn how to
use blood-drawing equipment properly. Experience will help the phlebotomist to choose the right equipment for each patient’s unique situation.
Phlebotomists will continually be exposed to re ned equipment that can
be utilized to get the best specimen with the least amount of trouble for
the patient.
REVIEW ACTIVITIES
1. Gauze is made of ______________________________________
_____________.
2. Gauze should be placed on the ______________________ rather
than on another surface when preparing to perform a venipuncture, in order to _______________________________________
___________________________.
3. A tourniquet is used to __________________________________
_____________.
4. A tourniquet should be cleaned and decontaminated frequently
because ______________________________________________
____________________.
5. Alcohol preps are used to ________________________________
_____________.
6. The size of the needle for a venipuncture is chosen based on
_____________________________________________________
___________________.
7. The three types of needles used most often by the phlebotomist
are:
a.
b.
c.
8. The two parts of a syringe are:
a.
b.
Copyright 2010 Cengage Learning. All Rights Reserved. May not be copied, scanned, or duplicated, in whole or in part. Due to electronic rights, some third party content may be suppressed from the eBook and/or eChapter(s).
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CHAPTER 4 Blood Collection Equipment
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9. Identify the additive in the following color-coded collection
tubes:
a. Lavender
b. Blue
c. Green
d. Red-brown
e. Gray
f. Pink
g. Yellow
DISCUSSION QUESTION
1. You go to Room 606 to draw the patient in bed 1. You also have
lab orders to draw the patient in bed 2. The patient in bed 1 is
an 80-year-old male with very small, fragile veins. The patient in
bed 2 is a 20-year-old male with large, healthy veins. Why is it
important to know the function and qualities of the equipment
in your collection tray?
95
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5
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“Our highly sophisticated
and well-controlled
laboratory technology is
useless if the specimens
presented for analysis
are already riddled with
error because of faulty
identi cation or poor
collection techniques.
Proper specimen collection
and specimen handling are
of the utmost importance,
for today, errors are
more likely to occur in
these areas than during
the laboratory procedure
itself.”
—National Committee
for Clinical Laboratory
Standards (1989)
Collection by Routine
Venipuncture
After studying this unit, it is the responsibility of the learner to be able to:
1. Organize a workload by stat, timed, ASAP, and routine orders.
2. Explain the importance of accurately identifying the patient.
3. Write the process for patient identi cation for outpatients and
inpatients.
4. Describe the process taken should the patient not have an inpatient or
outpatient identi cation armband.
5. Demonstrate the positioning of the patient for venipuncture.
6. Demonstrate and explain the process of venipuncture site selection.
7. Demonstrate appropriate venipuncture technique on an injectable
training arm.
8. Demonstrate appropriate venipuncture technique on a fellow student.
9. List the appropriate order of draw when using the evacuated collection
system.
10. List the rationale for each step of the venipuncture procedure.
11. Demonstrate on an injectable training arm the appropriate steps in
performing a venipuncture with the butter y winged infusion set.
12. List the appropriate order of draw when using the syringe/butter y
collection.
13. Explain the rationale for using a butter y/syringe rather than the evacuated
collection system.
Copyright 2010 Cengage Learning. All Rights Reserved. May not be copied, scanned, or duplicated, in whole or in part. Due to electronic rights, some third party content may be suppressed from the eBook and/or eChapter(s).
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antecubital in front of the elbow
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ASAP as soon as possible
aseptic free from infection
basilic vein prominent vein of the forearm
located on the inside edge of the antecubital
fossa
cephalic vein prominent vein in the forearm
utilized for venipuncture
edematous swollen due to an accumulation of
excess uid in the tissue
hematoma a swelling or mass of blood, usually
clotted, in an organ or tissue, caused by a
ruptured blood vessel due to injury
hemoconcentration increased localized blood
concentration of molecules such as cells,
proteins, and coagulation factors
lymphostasis lack of uid drainage in the lymph
system; stoppage of lymph ow
median cubital vein vein in the antecubital area
that is most commonly used for venipuncture
routine collection orders collected per individual
laboratory policy
stat at once, immediately; from the Latin statim
thrombosed occluded, or blocked, by a blood clot
timed collection orders collected at a speci ed
collection time
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V
PART 2 Blood and Urine Collection
enipuncture is the most frequently used method for obtaining a
blood sample for diagnostic testing. Because test results are highly
dependent on the quality of the specimen, the phlebotomist must
perform the venipuncture procedure at a high level of skill. In
addition to providing the laboratory with high-quality blood speci-
mens, the phlebotomist must also deliver a high-quality venipunc-
ture to the patient. The procedure must be performed so as to
cause minimal trauma to the patient.
This chapter will cover the procedure involved in collecting
a blood sample by performing a routine venipuncture. A routine
venipuncture assumes that the blood will be drawn from one of the
three prominent veins in the
the elbow; the median, the
phalic
lection system or the syringe/butter y collection system. Because
venipuncture is an invasive procedure, it must be performed in an
aseptic manner, free from the possibility of infection.
and demonstrate expertise in venipuncture technique at all
times. Because the student will experience a learning curve, the
rst several venipunctures may be unsuccessful. The venipuncture may not produce a blood sample, the needle may come out
of the puncture prematurely, or a hematoma may be produced.
These complicating factors will be addressed at the conclusion of
the chapter.
hands-on, experience that will provide the phlebotomist with
the opportunity to become pro cient in performing blood collections by venipuncture. Venipuncture is a skill, and it must be
practiced.
tures under the guidance of an instructor. An injectable training arm is an excellent tool to use when attempting the rst few
venipunctures. It allows the phlebotomist to practice tying the
tourniquet, to become familiar with handling the equipment, and
to commit to memory the steps in the collection process. Once
phlebotomy students feel comfortable with the injectable arm,
they may progress to practicing on a fellow student or an instructor. After an appropriate skill level is reached, the phlebotomy
veins. The veins will be punctured using the evacuated col-
The phlebotomist must collect high-quality blood samples
While classroom instruction is necessary, it is empirical, or
The phlebotomy student should perform all initial venipunc-
antecubital area which is in front of
median cubital, and the median ce-
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CHAPTER 5 Collection by Routine Venipuncture
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99
student may have the opportunity to obtain blood from a patient
under an instructor’s guidance.
As previously mentioned, it is very important that the patient
receive a venipuncture that is performed in an efficient, aseptic, and relatively painless manner. The preliminary steps to
performing the puncture are important and must be carried out
in a consistent manner.
ORGANIZATION OF WORKLOAD
Prioritize Orders
Collection orders are usually prioritized according to stat, timed, ASAP,
and routine orders. Should the phlebotomist have multiple collections
to perform, the orders must be placed in the proper order of collection.
Stat, or urgent, orders are to be collected immediately and the specimens
delivered to the lab immediately. Stat orders have priority in collection
over all other orders. They will always be drawn rst.
orders must be collected as close to the speci ed collection time as possible. After stats, timed collections are ranked next in priority
designated
ASAP
(as soon as possible) should be given third priority.
Routine collections receive last priority, and should be collected per
individual laboratory policy
.
Timed collection
. Orders
Copyright 2010 Cengage Learning. All Rights Reserved. May not be copied, scanned, or duplicated, in whole or in part. Due to electronic rights, some third party content may be suppressed from the eBook and/or eChapter(s).
Editorial review has deemed that any suppressed content does not materially affect the overall learning experience. Cengage Learning reserves the right to remove additional content at any time if subsequent rights restrictions require it.
Review the Collection Requisition
Before greeting a patient or entering a patient’s room, review the collection
order for the name of the patient, the test(s) ordered, the collection priority, and additional collection instructions or comments. Being prepared
beforehand will establish an atmosphere of con dence. Check the collection tray or your work area beforehand as well as the patient’s room to make
sure the appropriate equipment is available. Be sure to check for special
comments on the collection order or for posted instructions in the patient’s
room. Being alert to special considerations concerning the patient’s condition (for example, the patient who may be blind, deaf, or unable to speak
English) will help you to be more sensitive to the patient’s needs. It will
also allow you to appear more professional and well prepared.

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PART 2 Blood and Urine Collection
INTERACTING WITH THE PATIENT
Greet the Patient
Address the patient with a pleasant tone of voice and a smile. Introduce
yourself by rst name. In a hospital setting, state your purpose in being in
the patient’s room. If the patient is sleeping, attempt to wake the patient
up before you continue with the collection process. The patient may
ask questions concerning the tests that have been ordered. Instruct the
patient to direct such questions to the physician. If the patient should be
unconscious, always address the patient using the same manner and protocol as if he were awake.
Identify the Patient
Absolute identi cation of the patient is mandatory for every collection.
You must ensure accurate identification of the patient and the blood
samples throughout the collection process. Failure to do so may result in
serious consequences for the patient.
Inpatients
Compare the patient’s identi cation bracelet with the collection order
form. Compare the patient’s name, identification number, and date of
birth. The identi cation bracelet must be attached to the patient’s body.
A bracelet taped to the bed is not suf cient. Report any discrepancies or any
patients without bracelets to the appropriate person. Do not collect
any blood samples until the patient has been absolutely identi ed.
Outpatients
Ask the patient to state his or her name and birth date. Compare the stated
information with the collection order form. Report any discrepancies to the
appropriate person. Do not collect a blood sample until discrepancies have
been corrected. Some health care facilities issue the patient a temporary identi
cation armband. Verify that the armband contains correct information.
Unidenti ed Emergency Patients
A temporary means of identifying the patient must be established. The
temporary identi
cation must be attached to the patient’s body
. Do not
collect any blood samples until some identi cation has been attached to
the patient.
Copyright 2010 Cengage Learning. All Rights Reserved. May not be copied, scanned, or duplicated, in whole or in part. Due to electronic rights, some third party content may be suppressed from the eBook and/or eChapter(s).
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CHAPTER 5 Collection by Routine Venipuncture
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101
Verify Diet Restrictions and Time Requirements
Some tests have requirements, such as fasting or a special diet prior to collection of a blood sample. A timed collection should be collected at the
speci ed time. Should any of the diet or time requirements be neglected,
accurate test results will not be ensured. Do not collect a blood sample if such
requirements have not been met. Report discrepancies to the appropriate
person. Should a decision by the appropriate person be made to collect a
specimen even though the specimen requirements have not been met, collect the sample and document the discrepancy in the appropriate manner.
Position the Patient
The patient should be positioned so as to be comfortable before the
venipuncture is performed. In addition, the patient must be positioned to
facilitate comfortable collection for the phlebotomist.
Inpatients
Raise or lower the patient’s bed so that it will be at a comfortable level for
you when performing the venipuncture. The bed should be at a level
that will not create a strain on your back. Have the patient lie on his or
her back in a comfortable position. Ask the patient to hyperextend the
arm in a straight line from shoulder to wrist. The hand should be lower
than the shoulder. A pillow placed under the elbow makes a nice support for the arm. Lower the bed railing only on the side of the bed where
you will need to stand. Do not remove any patient restraints. Should the
patient be comatose or unable to change position, ask for assistance from
the patient’s nurse. Do not place the collection tray on the patient’s bed;
the patient may inadvertently knock the tray off the bed.
Copyright 2010 Cengage Learning. All Rights Reserved. May not be copied, scanned, or duplicated, in whole or in part. Due to electronic rights, some third party content may be suppressed from the eBook and/or eChapter(s).
Editorial review has deemed that any suppressed content does not materially affect the overall learning experience. Cengage Learning reserves the right to remove additional content at any time if subsequent rights restrictions require it.
Outpatients
The patient should be seated in the drawing chair. Have the patient
extend the arm to form a straight line from the shoulder to the wrist. The
arm should not be bent at the elbow. The arm rest attached to the drawing chair should support the patient’
s arm. Most drawing stations have a
bed where a blood draw can be performed. Use this bed if you think the
patient might faint.
Reassure the Patient
Explain the procedure to the patient. Explain that the pain will be brief.
Instruct the patient to remain as relaxed and calm as possible. If the

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PART 2 Blood and Urine Collection
patient appears to be frightened and anxious, have him or her focus on
breathing slowly and deeply.
PREPARING FOR THE VENIPUNCTURE
Select the Venipuncture Site
By making a preliminary assessment of the best veins in the patient’s
arms, you will be able to determine the most appropriate equipment for
performing the venipuncture. An assessment of the veins will determine
whether an evacuated system or a syringe setup will be more appropriate. In addition, you will be able to determine needle size. A preliminary
assessment will also identify any need to preheat the puncture site if the
blood vessels require dilating. The most frequently used veins for venipuncture are the super cial veins located in the forearm and hand. Please
refer to Figure 5-1.
The larger median cubital and cephalic veins are used most often. An
alternative choice may be the
take great care in selecting the basilic vein for venipuncture, as the vein
lies in close proximity to the median nerve and the brachial artery
dentally nicking the nerve or the artery may cause serious consequences
for the patient. However, other veins in the anterior surface of the forearm, as well as in the wrist and hand, are acceptable sites for venipuncture, provided that the selected site has no scarring, hematoma, burn, or
edema. The veins on the palm side of the wrist should not be used. Tendons and nerves lie close to the veins, and may be accidentally injured
while performing a venipuncture. The venipuncture site should also preferably not be on the side of a mastectomy or on the arm receiving intravenous therapy. There are, however, precautions that may be taken if it
is necessary to obtain a blood specimen from a site in either of these two
cases. These special considerations will be discussed later in this chapter.
If the potential venipuncture site involves any of these conditions, you
must try to select a different site.
The phlebotomist should depend primarily on touch and not sight
in locating and selecting a vein for venipuncture. A healthy vein will feel
“bouncy” to the touch when it is palpated. A
will not provide an adequate blood sample, feels cordlike and has no
spring or bounce. Applying a tourniquet and closing the patient’s hand
into a st will cause the veins to ll and become more prominent. Do not
pump the st. Opening and closing the st rapidly can cause localized
hemoconcentration, resulting in inaccurate test results.
basilic vein. Beginning students should
. Acci-
thrombosed vein, which
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Editorial review has deemed that any suppressed content does not materially affect the overall learning experience. Cengage Learning reserves the right to remove additional content at any time if subsequent rights restrictions require it.
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