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1 Anorectal Anatomy and Applied Anatomy
Clitoris
15
Urethra
Vagina
Anus
Sphincter ani
externus
Fig. 1.7 The perineal body is a complex fi bromuscular structure separating the urogenital organs and the anal sphincter
Ischiocavernosus
Bulbocavernosus
Transversus perinei
Levator ani
Gluteus maximus
and lies directly above puborectalis. Iliococcygeus originates from the lateral part of the obturator fascia and inserts into the coccyx. Innervation of the levator ani is by the pudendal nerve on its perineal aspect and the nerve to levator ani (S3, S4) on its pelvic surface [ 36 ].
1.2.9 Perineal Body
The perineal body , a complex fi bromuscular structure separating the urogenital organs and the anal sphincter, lies between the anal canal and the posterior four­chette in women and the penile bulb in men (Fig. 1.7 ). It is a vital constituent of both urinary and fecal continence mechanisms, supporting the perineum against increased abdominal pressure and is vulnerable to injury during childbirth. The perineal mus­cles (superfi cial and deep transverse perineal muscles) form the bulk of the perineal body as they cross the midline, but it also receives contributions from the external anal sphincter, bulbospongiosus muscle, and levator ani [ 39 ].
1.2.10 Blood Supply
Arterial supply to the anal canal is via the superior, middle, and inferior rectal arter­ies. Venous drainage is via the internal hemorrhoidal plexus to the superior rectal vein, to the inferior mesenteric vein, and to the portal system. The external hemor­rhoidal plexus drains via the middle rectal vein to the pudendal, internal iliac vein and fi nally the inferior vena cava and via the inferior rectal vein to the internal pudendal vein.
16
A.C.A. Murray and R.P. Kiran
1.2.11 Lymphatic Drainage
The lymphatic drainage of the rectum has been described above. The drainage of the upper anal canal, above the dentate line, is similar to the rectum, following the venous drainage path to the inferior mesenteric and internal iliac nodes. Below the dentate line, drainage follows the inferior rectal vein to the superfi cial inguinal nodes.
1.2.12 Perianal Skin
The perianal skin around the anal verge consists of keratinized stratifi ed squamous epithelium with eccrine glands, pilosebaceous units, and apocrine glands. The skin is pigmented and corrugated over the underlying EAS and allows for a large degree of stretch. It is exquisitely sensitive, with somatic innervation via the pudendal nerves S2, S3, and S4. Lymphatic drainage is to the superfi cial inguinal lymph nodes. The perianal skin is susceptible to any normal dermatological diseases.

1.3 Radiological Evaluation

Ultrasound is readily available, inexpensive, valuable in assessing rectal malig­nancy, sphincter complex anatomy, and benign anorectal disease. Its operator dependency does however, limit its consistency.
1.3.1 Endorectal Ultrasound (ERUS)
Rectal imaging requires an ultrasound transducer probe covered by a water-fi lled balloon. The patient is given an enema and examined in the left lateral decubitus position. ERUS images are able to demonstrate fi ve main layers: (1) balloon mucosa interface, (2) mucosa and muscularis mucosa, (3) submucosa, (4) muscularis propria, and (5) interface with fat (Fig. 1.8a, b ).
Occasionally there is an additional layer seen, the submucosa being separated into inner circular and outer longitudinal muscle. ERUS is a valuable tool for assess­ing the depth of tumor invasion in rectal cancer; however, the detection of malignant involvement of perirectal lymph nodes is less accurate.
1.3.2 Endoanal Ultrasound
Endoanal ultrasound is valuable in the assessment of benign and malignant disease as well as providing detailed views of the anal sphincter complex in the assessment of incontinence. Similar to the ERUS, a transducer is placed inside the anal canal
1 Anorectal Anatomy and Applied Anatomy
a
17
b
NORMAL GI WALL
1st
2st
3rd
a
4th
b c
5th
Fig. 1.8 ( a ) ERUS showing T1 rectal cancer confi ned to the mucosa and superfi cial submucosa. SM submucosa, MP muscularis propria, SR serosa. ( b ) Corresponding ERUS and histological lay- ers (from Siddiqui et al. [
40 ], with permission)
HISTOLOGYEUS
Superticial mucosa
Deep mucosa
Muscularis mucosa
Submucosa
Clrcular muscularis propria
Connective tissue Longitudinal muscularts propria
Serosa / Adventitia
but covered with a water-fi lled plastic cap. The probe is introduced to cover the entire anal canal and it is viewed at the upper, mid, and lower anal levels. The upper level is delineated by puborectalis, the IAS, and EAS seen at the mid-level by a hypoechoic and hyperechoic band, respectively. The anal vascular cushions of vari­able thickness can also be visualized at this point. At the distal anal canal, the IAS is no longer seen, only the hyperechoic EAS and surrounding fatty tissue. Defects in the sphincters can be accurately assessed and are seen as missing segments of the circumferential band, or in the EAS these may be seen as hypoechoic or amorphous and mixed echogenicity defects [ 41 ]. In complete sphincter disruption, there will be a complete gap in the sphincter concentric ring with scar tissue replacing vital mus­cle. Fistula tracts can be seen as hypoechoic defects and their paths can be traced in relation to the sphincter mechanism. More recently, 3D ultrasound images have shown reliable assessment of anal sphincter complex morphology with the ability to capture anal sphincter volume [ 38 ].
1.3.3 MRI
The two main approaches for MRI are with an external or an endoanal coil. The benefi t of the latter is greater spatial discrimination, but this is at the expense of breadth of fi eld. This then suits endocoil MRI to conditions of the anal canal specifi ­cally sphincter defects, abscess, and complex fi stulae. MRI with an externally placed coil provides valuable information about rectal and anal anatomy within the general landscape of the pelvic anatomy. Endocoil MRI has provided colorectal surgeons with a more detailed anatomical understanding of the sphincter complex and pelvic musculature [
26 ].
18
A.C.A. Murray and R.P. Kiran

1.4 Clinical Evaluation

Following a thorough history and discussion with the patient, a general and abdomi­nal examination is performed. A routine digital rectal examination should be per­formed on all patients presenting with anorectal or lower gastrointestinal symptoms. After gaining consent, the patient is placed in a comfortable position for examina­tion of the perianal region, perineum, anus and rectum. The left lateral decubitus position is most commonly used, but patients can also be examined in the genucu­bital or lithotomy position. It is important to obtain consent and ensure patient com­fort and dignity, optimum lighting, and the presence of a chaperone. Parting the buttocks allows for adequate inspection, and here the skin should be examined for dermatological disease and external stigmata of perianal or gastrointestinal disease. The surgeon should specifi cally look for any previous scars, any swellings or ulcer­ation, external fi stula openings on the skin, and any obvious pus, mucus, or bloody discharge. A closer look at the anoderm may reveal the characteristic ulcer of an anal fi ssure. The patient should be asked to strain, looking for rectal prolapse, pro­lapsing hemorrhoids or anal polyps, and perineal descent.
If the patient will tolerate digital rectal examination, a gloved and lubricated index fi nger is inserted gently into the anus. The fi nger is advanced meanwhile assessing for any discomfort. The areas examined are the wall of the anal canal and rectum; the anterior aspect allows palpation of the prostate in men and posterior cervix in women, and the coccyx can be felt posteriorly. Resting and then voluntary sphincter tone can be assessed. Examination of the gloved fi nger upon withdrawal may show signs of pus, blood, mucus, or melena.
1.4.1 Proctoscopy/Anoscopy
This is a quick and inexpensive method to examine the anus and anorectum to approximately 10 cm following digital rectal examination. This should be performed in all patients with proctological signs or symptoms when tolerated. In addition, it allows the surgeon to perform outpatient treatments of hemorrhoidal disease.
The anoscope should be well lubricated and assembled before beginning the procedure. The introducer of the anoscope is inserted slowly into the anal canal to the hilt. The introducer is removed and a close inspection of the anal canal mucosa made. Here any mucosal infl ammation or ulceration, any anal polyps, or low tumors in the anal canal should be visible. Hemorrhoids will be seen above the dentate line as purple-tinged grapelike swellings that extend into the lumen of the anal canal. They may bleed on contact.
1.4.2 Hemorrhoid Injection Therapy
Before any treatment in clinic, the patient should be warned of potential post­procedure discomfort and minor bleeding. After informed consent, the correct equipment should be prepared.
1 Anorectal Anatomy and Applied Anatomy
Injection of hemorrhoids with 5 % oily phenol (arachis oil) requires a long needle and syringe and anoscope. With the hemorrhoids demonstrated and the den­tate line identifi ed on anoscopy, the needle is advanced and, using a gentle, but purposeful, stabbing motion, pierces the submucosa. The patient should not feel any pain if one is correctly above the dentate line. If any sensation is felt, the nee­dle needs to be removed and reinserted cranially. Injection of the sclerosant should raise a bleb of mucosa. Approximately 3–5 ml is injected into each visible hemor­rhoid taking care not to inject too deep anteriorly as the misplaced injection of phenol can cause prostatitis, perineal pain, and in severe cases urinary sepsis.
19
1.4.3 Rubber Band Ligation
The suction hemorrhoid applicator ensures ease of application of the rubber band. The applicator is introduced through the anoscope, and with suction of the mucosa overlying a visible hemorrhoid, a rubber band is deployed over the hemorrhoid bundle. Although a dull sensation of pressure within the anal canal is expected, sharp pain signifi es incorrect application of the band at or below the dentate line suggesting the need for removal. The bands usually fall out after 5–10 days.
1.4.4 Rigid Sigmoidoscopy/Proctosigmoidoscopy
This is an essential diagnostic tool in the outpatient and operating room setting and provides a view of the rectal mucosa for the evaluation of infl ammation, ulceration, or bleeding. In addition rectal polyps and tumors can be visualized and their dis­tance from the anal verge measured. In the acute setting, a sigmoid volvulus can often be conservatively managed by insertion of a fl atus tube via a proctosigmoido­scope with subsequent decompression of the bowel.
With the patient comfortable, consented, and chaperoned, the lubricated rigid sigmoidoscope is gently inserted. After entry into the anal canal, the introducer is removed. The light source should allow direct visualization of the anal canal and then the rectal mucosa. The rigid scope is gradually advanced with the gentle insuffl ation of the rectum with air as required to view the walls clearly while constantly viewing the rectal lumen. This helps advancement of the tube in such a way that it follows the course of the rectum within the pelvis and reduces the risk of mucosal damage or rectal perforation. With the patient in the left lateral position, the rigid sigmoidoscope is fi rst angled toward the sacrum and then advanced with an anterior tilt at the anorec­tal angle. The sigmoidoscope in an empty rectum can optimally reach 20–22 cm.
1.4.5 Flexible Sigmoidoscopy
Flexible sigmoidoscopy allows visualization of the rectum and distal colon to a greater distance than the rigid tube. In a comfortable and well-prepped patient, the entire sigmoid, left colon, and splenic fl exure can be seen. With the patient in the
20
A.C.A. Murray and R.P. Kiran
left lateral position, the fl exible fi ber-optic tube is inserted into the anal canal. The rectal mucosa is pink and folded. Upon air insuffl ation, the walls expand outwards to offer a clear view of the lumen. The valves of Houston can be negotiated to allow more proximal examination. The majority of adenocarcinomas are found in the left colon (27 % rectum, 7 % rectosigmoid junction, 20 % sigmoid, 3 % descending colon). Flexible sigmoidoscopy can be used for biopsy, polypectomy, hemostasis of actively bleeding lesions, and tattooing of lesions.
1.4.6 Positioning in the OR
Optimal positioning in the OR allows for good exposure of the relevant anatomy, unimpeded access for the surgeon in order to perform the procedure and safety for the patient. Padded supports provide protection against pressure injuries, for exam­ple, common peroneal nerve injuries in the lithotomy position due to compression from the supporting bar on the nerve as it wraps around the fi bula. Aside from the supine position, some of the most common positions used are lithotomy, Lloyd­Davies, and prone jackknife (Fig. 1.9 a–c ).
Fig. 1.9 ( a ) Lithotomy. ( b ) Lloyd-Davies. ( c ) Prone jackknife (from Givel [ 42 ], with kind permission)
1 Anorectal Anatomy and Applied Anatomy
21

1.5 Common Anorectal Conditions and Applied Anatomy

1.5.1 Fissure
Fissure in ano is a common and problematic benign anorectal condition, which makes up 15 % of proctology consultations [ 43 ]. The condition is defi ned as a linear tear or ulcer in the anorectal squamous epithelium that occurs beyond the dentate line, at the anal verge. A chronic anal fi ssure is one that persists for more than 6 weeks despite medical management and may have the classic features of rolled edges, exposure of the internal anal sphincter fi bers, and a sentinel tag or anal papilla. Fissures are usually in the posterior midline, but can also be found anteriorly, later­ally, or in multiple locations. The location of the fi ssure may suggest a cause, with lateral fi ssures suggesting an underlying chronic disease process such as Crohn’s, ulcerative colitis, syphilis, tuberculosis, leukemia, cancer, or HIV. It is hypothesized that hypertonia and relative sphincter hypoperfusion result in ischemia of the poste­rior anal canal which causes pain and poor wound healing associated with a chronic anal fi ssure. This may be further exacerbated by recurrent minor anal trauma.
Digital rectal examination and proctoscopy may not be tolerated due to pain. If permitted, the gloved fi nger may identify a tender ridge at the position of the fi s­sure, while proctoscopy reveals a raw ulcer or split in the anoderm. Longstanding fi ssures may reveal fi bers of the internal anal sphincter and sentinel tag(s) or anal papilla(e). Patients will exhibit varying degrees of sphincter spasm on examination consistent with the hypothesized pathophysiology of hypertonic sphincter.
The fi rst steps to treating any acutely symptomatic fi ssure in ano are conserva­tive, with analgesia, stool softeners and sitz baths. Many will heal spontaneously; however, if that fails, topical ointments which act to reduce internal anal sphincter tone and improve local blood fl ow such as nitroglycerine, diltiazem, or nifedipine are used. Surgical treatment options include injection of Botox, fi ssurectomy, lateral internal sphincterotomy (LIS), and anal advancement fl aps. Lateral internal sphinc­terotomy can be performed using an open or closed technique and involves a con­trolled division of the internal sphincter. In LIS the internal sphincter is divided from its caudal edge, to a variable proximal distance, but no further than the dentate line. Over-extensive cutting can lead to incontinence.
1.5.2 Hemorrhoids (from the Greek Haem = Blood, Rhoos = Flow.
Piles in Latin Pila = a Swelling or Ball)
The anal cushions are physiological highly vascular structures, present in all indi­viduals, and located above the dentate line at the transition zone. Their vascular nature enables them to vary in size and they become engorged with blood during periods of raised intra-abdominal pressure. This aids closure of the anal canal, con­tributing to the maintenance of continence [ 44 ]. The vascular cushions consist of the lining mucosa, the underlying vascular plexus, smooth muscle, and the connective
22
A.C.A. Murray and R.P. Kiran
Conjoined
longitudinal
muscle
Hemorrhoidal
venous
plexus
Muscularis
mucosa
Treitz’s muscle
External sphincter
Fig. 1.10 The vascular cushions consist of the lining mucosa, the underlying vascular plexus, smooth muscle, and the connective tissue scaffolding which anchors the hemorrhoids to the inter­nal sphincter and the conjoined longitudinal muscle
tissue scaffolding which anchors the hemorrhoids to the internal sphincter and the conjoined longitudinal muscle (Fig. 1.10 ) [ 45 ]. The anal submucosal smooth mus- cle, or Treitz’s muscle, originates from the longitudinal muscle and passes through the internal anal sphincter anchoring the hemorrhoids to the submucosa. Smooth muscle fi bers interdigitate the vascular plexus forming a scaffold support, which is susceptible to damage in hemorrhoidal disease. Described by Thomson in 1975, the anal cushions are classically located in the left lateral, right anterior, and right pos­terior positions (3, 7, 11 o’clock) [ 46 ]; however, only 19 % of patients are found to have this arrangement of hemorrhoids.
1.5.3 Anal Cushion
The hemorrhoidal plexus within the submucosa is also called the corpus caverno­sum recti [ 47 ] as it is a dense vascular network which fi lls and engorges, similar to erectile tissue. The arteriovenous network does not have interposing capillaries. When patients notice fresh red rectal bleeding, the pH when tested confi rms mainly arterial bleeding. The vascular plexus is primarily fed by terminal branches of the
1 Anorectal Anatomy and Applied Anatomy
23
superior hemorrhoidal artery with anastomoses to the middle and inferior hemor­rhoidal arteries [ 46 ]. Transanal hemorrhoidal dearterialization (THD) and Doppler- guided hemorrhoidal artery ligation (DG-HAL) are surgical techniques to treat hemorrhoids and are targeted at restricting arterial blood fl ow.
Venous drainage follows that of the arterial supply from superior rectal to the inferior mesenteric and the middle rectal and inferior rectal (via the pudendal) to the internal iliac vein and thus to the inferior vena cava. The anal canal is an example of a portosystemic anastomosis, whereby the superior rectal vein drains into the portal system (via the inferior mesenteric vein) and the middle and inferior rectal veins drain into the systemic circulation (internal iliac and pudendal vein).
Hemorrhoids can be classifi ed as either internal or external, depending on their relation to the dentate line. Internal hemorrhoids derive from the embryonic endoderm and are lined with the columnar epithelium of the anal mucosa. The internal hemor­rhoidal plexus above the dentate line is drained by the middle rectal vein to the inter­nal iliac vein. The external hemorrhoids are derived from ectoderm and are covered by squamous epithelium. The exterior hemorrhoidal plexus lies below the dentate line and drains via the inferior rectal vein to the pudendal vein and hence to the internal iliac vein. Either can prolapse and be identifi ed from the anal verge on examination.
The inferior rectal nerve and perineal nerve (branches of the pudendal nerve) provide somatic innervation distal to the dentate line. Proximally the only sensation is supplied by visceral afferent fi bers which join the inferior hypogastric plexus. Clinically this explains why painful hemorrhoids tend to be those that are either external hemorrhoids or prolapsed or thrombosed internal hemorrhoids.
The etiology of hemorrhoids is still debated. It is proposed that if the anal cush­ions are distally displaced, by straining, sustained increased intra-abdominal pres­sure or laxity of the supporting structures with age (smooth muscle replaced with connective tissue) and increase an in size, they may become more susceptible to trauma and shearing forces and symptomatic hemorrhoids may then occur [ 48 , 49 ]. Hypertonia of the internal anal sphincter has also been postulated [ 50 ]. Histologically, hemorrhoids show hypertrophy of the subepithelial smooth muscle of the anal cush­ions, thickened intramucosal vessels, and stromal hyperplasia. Submucosal thicken­ing, in combination with thickening of the internal anal sphincter, has also been shown on ultrasonography. Based on the degree of prolapse, hemorrhoids are graded as grade I hemorrhoids that do not prolapse, grade II hemorrhoids that prolapse and spontaneously reduce, grade III hemorrhoids that prolapse but require manual reduction, and grade IV that remain persistently prolapsed [ 51 ]. Treatment depends on the degree of prolapse and severity of symptoms, with grades I and II generally amenable to local procedures in the clinic.
1.5.4 Perianal Sepsis
The majority of perianal sepsis is thought to be cryptoglandular in origin; it is hence vital to understand the anatomy of the anal glands. Above the dentate line are crum­pled folds in the anal mucosa called the columns of Morgagni. At the base of these lie the anal crypts and these communicate with ducts that drain the anal glands.
24
A.C.A. Murray and R.P. Kiran
The anal glands normally produce mucin for lubrication of the anal canal for the smooth passage of stool; however, if blocked, for example, by foreign material, infection may ensue. Anal glands lie mostly in the submucosal plane, but can also be found at the IAS, communicating with the intersphincteric space and extending to the conjoined longitudinal muscle.
1.5.5 Anal Glands
There are a number of potential spaces for infection to develop and these are called the perirectal spaces. Infection either can seed and spread upward from the perianal region or can spread downwards from the pelvic cavity. The perirectal spaces that can develop abscesses include:
1. Perianal space: Surrounds the anal canal at the anal verge. Its borders include the
subcutaneous fat laterally and the anal canal medially. This space connects with
the intersphincteric space.
2. Intersphincteric space: Between the IAS and EAS.
Supralevator
abscess
postanal-space
Fig. 1.11 The perirectal spaces that can develop abscesses include perianal space, intersphincteric space, ischiorectal space, and supralevator space
Deep
abscess
Perianal abscess
Intersphincteric
abscess