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- •Carbon Based Nanomaterials for Drug Delivery
- •Preface
- •Acknowledgements
- •Contents
- •Editor and Contributors
- •Abbreviations
- •1.2 Market Statistics
- •Carbon-Based Nanomaterials: An Overview
- •1. Introduction
- •1.1 Evolution of Carbon-Based Nanomaterials
- •2. Carbon-Based Nanostructures
- •2.1 Fullerene
- •2.2 Carbon Nanotubes (CNTs)
- •2.4 Graphene
- •2.5 Nanodiamonds (NDs)
- •2.6 Nano-Onions (CNOs)
- •2.7 Nanohorns (CNHs)
- •2.8 Carbon Dots (CDs)
- •2.9 Nanoporous Activated Carbon
- •3. Synthesis Techniques
- •4. Properties of Carbon-Based Nanomaterials
- •4.1 Physicochemical Properties
- •4.2 Thermal Properties
- •4.3 Mechanical Properties
- •4.4 Optoelectronic Properties
- •4.5 Antimicrobial Properties
- •4.6 Biological Properties
- •5. Applications of Carbon-Based Nanomaterials
- •5.1 Environmental Remediation
- •5.2 Agriculture
- •5.3 Biofuel
- •5.4 Energy Storage
- •5.5 Biomedical Applications
- •6. Challenges and Future Perspectives
- •7. Concluding Remarks
- •References
- •Carbon-Based Nanostructured Materials: Designing, Properties and Applications
- •1. Introduction
- •2.1 Zero-Dimensional Carbon-Based Nanostructures (0D)
- •2.2 One-Dimensional Carbon-Based Nanostructures
- •2.3 Two-Dimensional (2D) Carbon-Based Nanostructures
- •2.4 Three-Dimensional (3D) Carbon-Based Nanostructures
- •3.1 Chemical Vapor Deposition
- •3.2 Hydrothermal and Solvothermal Techniques
- •3.3 Microwave-Assisted Technique
- •3.4 Chemical Oxidation Synthesis
- •4. Properties of Carbon-Based Nanostructured Materials
- •4.1 Thermal Properties
- •4.2 Mechanical Properties
- •4.3 Optoelectronic Properties
- •4.4 Antimicrobial Properties
- •4.5 Biological Properties
- •5. Applications of Carbon-Based Nanostructured Materials
- •5.2 Antibacterial and Antiviral Applications
- •5.3 Theragnostic
- •5.4 Wound Healing
- •5.5 Tissue Engineering
- •5.6 Drug Delivery
- •5.7 Biosensing
- •6. Challenges and Future Perspectives
- •7. Concluding Remarks
- •References
- •Drug Delivery System and Technologies
- •1. Introduction
- •2. Drug Delivery System
- •2.1 Conventional Drug Delivery System
- •2.2 Advanced Drug Delivery System
- •2.3 Controlled and Sustainable Drug Delivery System
- •3. Drug Delivery Technologies
- •3.1 Active and Passive Drug Delivery
- •3.2 Smart Drug Delivery
- •3.3 Intravenous and Extravaneous Drug Delivery
- •3.4 Various Types of Delivery Technologies
- •4. Challenges and Future Perspectives
- •5. Conclusion
- •References
- •Carbon-Based Nanomaterials for Drug Delivery: Past, Present, Future Directions
- •1. Introduction
- •3. Current Status in Drug Delivery by CNMs
- •3.1 Graphene-Based Nanomaterials in Drug Delivery
- •3.4 Nanodiamond Based Drug Delivery Systems
- •3.5 Nano-Onions in Drug Delivery
- •3.6 Nanohorns in Drug Delivery
- •3.7 Fullerene in Drug Delivery
- •4. Challenges and Future Perspective
- •5. Conclusions
- •References
- •Carbon Nanomaterial-Incorporated Supramolecular Drug Delivery
- •1. Introduction
- •2. Different Carbon Nanomaterials in Drug Delivery
- •2.1 Carbon Nanotubes (CNTs)
- •2.3 Graphene
- •2.4 Carbon Quantum Dots
- •2.5 Fullerene
- •2.6 Carbon Nanohorns
- •2.7 Carbon Nano-Onions
- •2.8 Nano-Diamond
- •3. Supramolecular Chemistry in Drug Delivery
- •3.1 Principles of Supramolecular Chemistry
- •3.3 Applications of Supramolecular Biomaterials
- •4. Carbon Nanomaterial-Incorporated Supramolecular Drug Delivery
- •5.1 Cyclodextrins
- •5.2 Calixarenes
- •5.3 Cucurbituril
- •5.4 Pillarenes
- •5.5 Crown Ether
- •6. Toxicity Concerns of Carbon Nanomaterials
- •7. Improving the Effectiveness of Nanoparticle Systems
- •8. Future of Nanomedicine
- •9.1 Challenges
- •9.2 Future Perspectives and Opportunities
- •9.3 Conclusions
- •References
- •Carbon Nanomaterial-Based Polymeric Nanocomposites for Drug Delivery
- •1. Introduction
- •2.1 Carbon Quantum Dot-Based Polymer Nanocomposite
- •2.2 Carbon Nanotube-Based Polymer Nanocomposite
- •2.3 Graphene Quantum Dot-Based Polymer Nanocomposite
- •2.5 Fullerene-Based Polymer Nanocomposite
- •2.6 Nanodiamond-Based Polymer Nanocomposite
- •3. Drug Delivery Systems Using Carbon Nanomaterial
- •3.1 Anticancer Drug Delivery
- •3.3 Infectious Disease Drug Delivery
- •3.4 Topical Drug Delivery
- •3.5 Brain Drug Delivery
- •3.6 Oral Drug Delivery
- •4. Challenge and Future Perspectives
- •5. Conclusion
- •References
- •Carbon Nanomaterial-Incorporated Polysaccharide-Based Nanocomposite for Drug Delivery
- •1. Introduction
- •1.1 Drug Delivery
- •1.2 Carbon Nanomaterials
- •1.3 Polysaccharide-Based Nanocomposite
- •2.1 CN-Incorporated Alginate-Based Nanocomposite
- •2.2 CN-Incorporated Cellulose-Based Nanocomposite
- •2.3 CN-Incorporated Chitosan-Based Nanocomposite
- •2.4 CN-Incorporated Dextran-Based Nanocomposite
- •2.5 CN-Incorporated Hyaluronic Acid-Based Nanocomposite
- •2.6 CN-Incorporated Starch-Based Nanocomposite
- •2.7 CN-Incorporated Pectin-Based Nanocomposite
- •2.8 CN-Incorporated Guar Gum-Based Nanocomposite
- •2.9 CN-Incorporated Agarose-Based Nanocomposite
- •2.10 CN-Incorporated Carrageenan-Based Nanocomposite
- •2.11 CN-Incorporated Glucomannan-Based Nanocomposite
- •3. Challenges and Future Prospective
- •4. Concluding Remarks
- •References
- •Graphene-Based Nanomaterials for Drug Delivery
- •1. Introduction
- •1.1 Challenges in Conventional Drug Delivery Systems
- •1.2 Overview of Nanomaterials for Drug Delivery
- •1.3 Role of Graphene-Based Nanomaterials in Drug Delivery
- •2. Synthesis of Graphene
- •2.1 Chemical Reduction Method
- •2.2 Thermal Reduction
- •2.3 Electrochemical Reduction
- •2.4 Chemical Vapor Deposition Method
- •2.5 Mechanical Exfoliation
- •2.6 Epitaxial Growth Method
- •2.7 Growth in Solvothermal and Hydrothermal Systems
- •2.8 Electrochemical Deposition
- •3. Types of Graphene-Based Materials
- •3.1 Graphene Quantum Dots, (GQDs)
- •3.2 Graphene Oxide (GO)
- •3.3 Graphene Nanoribbons (GNRs)
- •3.4 Oxidized Graphene Nanoribbons
- •4. Graphene Functionalized Materials for Drug Delivery
- •4.1 In Bone Tissue Regeneration
- •4.2 In Neural Regeneration
- •4.3 In Photodynamic and Photothermal Therapy
- •4.4 In Enhancing Cellular and Humoral Immunity
- •4.6 Miscellaneous
- •5. Challenges and Future Perspective
- •6. Conclusion
- •References
- •Carbon Quantum Dots Based Materials for Drug Delivery
- •1. Introduction
- •2. Synthesis Process of Carbon Quantum Dots
- •2.1 Top-Down Approaches
- •2.2 Bottom-Up Approaches
- •2.3 Microwave-Assisted Method
- •2.4 Electrochemical Method
- •2.5 Laser Ablation Method
- •2.6 Pyrolysis Method
- •2.7 Template-Assisted Method
- •4. Challenges and Future Perspective
- •5. Concluding Remarks
- •References
- •Carbon-based Nanocarriers for Sustained Drug Release in Dentistry
- •1. Introduction
- •2.1 Oral Mucosa Structure
- •2.2 Sites for Drug Delivery
- •2.3 Permeability
- •3. Local Drug Delivery for Dental Diseases
- •3.1 Odontogenic Infection
- •3.2 Non-odontogenic Infection
- •4. Bio-adhesive Nanoparticles: Novel Treatment Modality
- •4.1 Bio-adhesive Nanoparticles
- •4.2 Mechanism of Bioadhesion
- •5.1 Carbon Nanotubes
- •5.2 Graphene
- •5.3 Nanodiamonds
- •5.4 Fullerenes
- •5.5 Porous Carbon
- •5.6 Carbon Dots
- •6. Drug Delivery Systems Based on CBNs
- •6.2 Immediate Drug Delivery System (IDDS)
- •6.3 Sustained-release Drug Delivery Systems
- •6.4 Controlled Drug Delivery System (CDDs)
- •8. Conclusion
- •References
- •Fullerene Based Materials for Drug Delivery
- •1. Introduction
- •2. Types of Fullerene Derivatives
- •2.1 Exohedral Fullerene Derivatives
- •2.2 Endohedral Fullerene Derivatives
- •2.3 Surface Derivatized Fullerenes
- •3. Interaction of Fullerene Derivatives for Drug Delivery
- •4. Fullerene Based Materials for Drug Delivery
- •4.1 Nucleic Acid Delivery
- •4.2 Peptide Delivery
- •4.3 Topical Drug Delivery
- •4.4 Infectious Diseases Drug Delivery
- •4.5 Anticancer Drug Delivery
- •4.7 Brain Drug Delivery
- •4.8 Ocular Drug Delivery
- •5. Challenges and Future Perspectives
- •6. Concluding Remarks
- •6.1 Abbreviations
- •References
- •Graphene Quantum Dots-based Nanomaterials for Drug Delivery
- •1. Introduction
- •2. Synthesis of GQDs
- •3. GQD’s Properties for Drug Delivery
- •3.1 Optical Properties
- •3.2 Physicochemical Properties
- •3.3 Mechanical Properties
- •3.4 Biocompatibility and Cytotoxicity
- •4. Characterization of GQDs-Based Nanomaterials
- •4.1 Characterization of Multifunctional GQDs-Based Nanomaterials
- •5.1 Strategies for Developing Medication Delivery Systems Based on GQD
- •5.2 PH-responsive Drug Delivery Systems (GQD-DDSs)
- •5.3 Targeted Drug Delivery Using Ligand-Based GQDs as a Mediator
- •5.4 Improvement of Medicines’ Pharmacological Properties Using GQDs
- •5.5 Enhancing Cytotoxicity with GQD-DDS
- •7. Applications of Chiral GQDs
- •10. Challenges and Future Perspectives
- •11. Conclusions
- •References
- •Carbon Nano-onions for Drug Delivery
- •1. Introduction
- •2. Carbon Nano-Onion: A Multi-Layered Nanocarrier
- •3. Synthesis of Carbon Nano-Onions
- •3.1 Annealing Method
- •3.2 Carbon Ion Implantation Method
- •3.3 Arc Discharge Method
- •3.4 Carbon Vapour Deposition Method
- •3.5 Pyrolysis Method
- •6. Carbon Nano-Onions in Drug Delivery
- •6.1 Delivery of Therapeutic Agents
- •6.2 Delivery of Targeting Agents
- •6.3 Delivery of Imaging Agents
- •7. Challenges and Future Perspectives
- •8. Concluding Remarks
- •References
- •Chitosan/Carbon Nanocomposites in Drug Delivery and Cardiovascular Diseases
- •1. Introduction
- •1.1 Drug Delivery
- •1.2 Cardiovascular Diseases
- •1.3 Chitosan and Its Properties
- •1.4 Chitosan/Carbon Nanocomposites
- •2. Chitosan/Carbon Nanocomposites in Drug Delivery
- •3. Chitosan/Carbon Nanocomposites in CVDs
- •3.1 Chitosan-Based Scaffolds
- •3.2 Chitosan in Cardiac Tissue Engineering
- •3.3 Chitosan-Based Cell Therapy
- •3.4 Chitosan-Based Gene Delivery
- •3.5 Chitosan-Protein Interaction
- •4. Challenges and Future Perspective
- •5. Concluding Remarks
- •References
- •Graphene Reinforced Chitosan Nanocomposites for Drug Delivery
- •1. Introduction
- •2. Chitosan: Structure and Properties
- •3. Graphene: Structure, Types and Properties
- •4.1 Electrospinning Method
- •4.2 Sol–gel Method
- •4.3 Solution Mixing Method
- •4.4 In-situ Polymerization Method
- •5.2 Chitosan/Graphene Aerogels
- •5.3 Chitosan/Graphene Hydrogels
- •5.4 Chitosan/Graphene Thin Films
- •6.1 Oral Drug Delivery
- •6.2 Mucosal Drug Delivery
- •6.3 Transdermal Drug Delivery
- •6.4 Parenteral Drug Delivery
- •7. Challenges and Future Perspectives
- •8. Concluding Remarks
- •References
- •1. Introduction
- •2. Functionalization of CNFs
- •2.1 The Need for Functionalization

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Carbon-Based Nanostructured Materials: Designing, Properties and Applications
Verónica Esparza-Cordero, Camila Castanedo-Carrillo,
Alain R. Picos-Benítez, and Blanca L. Martínez-Vargas
Abstract Carbon (C) is considered the most abundant element on the planet, so
it is desirable to be applied to different studies. Carbon nanostructures are present
in several shapes and forms, each with different properties that their nanometric
size can modify. Carbon nanotubes (CNTs) and graphene show unique electronic,
optical, mechanical, and chemical properties, which has led to the development of
new nanomaterials with unique properties and new devices. The application of these
novel nanomaterials will depend mainly on the synthesis method, where chemical
vapor deposition is the most used, using graphite as the primary precursor. The
applications of CNTs and graphene are in the electronic industry for storage energy
in electronic devices; some other multifunctional materials are used as catalysts
and sensors, and carbon-based nanomaterials are reported to be used in the medical
area due to their antibacterial properties. Its small size and weight suggest potential
uses in wearable equipment, sensors, devices, and medications that can contribute to
diseases such as cancer. This chapter describes the different structures of carbon, its
synthesis methods currently used, and the main properties that make it attractive in
health sciences areas.
Keywords Carbon-based nanostructure
Biosensing·Drug delivery
· Synthesis techniques · Wound healing ·
Abbreviations
˙OH Hydroxyl
μs Microseconds
V. Esparza-Cordero
Unidad Profesional Interdisciplinaria de Ingeniería Campus Zacatecas, Instituto Politécnico
Nacional, 98160, Zacatecas, México
C. Castanedo-Carrillo · A. R. Picos-Benítez · B. L. Martínez-Vargas (
Centro de Estudios Científicos y Tecnológicos No. 18, Instituto Politécnico Nacional, 98160,
Zacatecas, México
e-mail: bmartinezv@ipn.mx
B
)
39

40 V. Esparza-Cordero et al.
CD Carbon dots
CNH Carbon nanohorns
CNM Carbon nanomaterials
CNO Carbon nano-onions
CNT Carbon nanotubes
CPT Camptothecin
CS Chitosan
DNA Deoxyribonucleic acid
DOX Doxorubicin
GO Graphene oxide
GQDs Graphene quantum dots
HA Hyaluronic acid
HCoV-229E7 Human coronavirus
HIV-1 Immunodeficiency virus
MRSA Methicillin-resistant S. aureus
MWCNT Multi-walled carbon nanotubes
ND Nanodiamonds
NIR Near-infrared
ns Nanoseconds
PDT Photodynamic therapy
PL Photoluminescence
PLLA Poly-L-lactic acid
PTT Photothermal therapy
rGO Reduced graphene oxide
RNA Ribonucleic acid
ROS Reactive oxygen species
S42 Schwann cells
SWCNH/SWNHs Single-walled carbon nanohorns
1 Introduction
Carbon-based materials have multiple applications derived from their different crystalline structures. Furthermore, as it is the most abundant element on the planet, it can
be used individually or in conjunction with other hybrid materials to be applied as a
base for solar cells, as a photocatalyst, in the design and construction of biosensors,
in the health area, as already mentioned. Technological advances have allowed the
development of new sciences, such as nanoscience and nanotechnology, which have
allowed the study and development of nanoscale materials that range from structures
on metal based to carbon based. Regarding those that are carbon based, we find
materials such as fullerene, graphene and its derivatives, nanotubes, nanodiamonds,
carbon quantum dots, and all derivatives, materials due to their physical, chemical,
and biological properties range from their size, its chemical bonds, the surface area,

Carbon-Based Nanostructured Materials: Designing … 41
the ability to combine with other chemical elements, its biocompatibility, its photoluminescent capacity, high resistance, and electrical conductivity [1, 2]. They are
undoubtedly materials with various applications in the medical area for the diagnosis and treatment of diseases, through their use for the marking of tumor cells or
for their use in the arrest of target molecules and creation of biosensors, as well as the
destruction of the same by photodynamic or photothermal therapy, as well as to be
used as drug vehicles to treat various pathologies of various kinds, including infectious diseases due to their antimicrobial and antiviral capacity, in addition to being
used as a scaffolding structure for the development and proliferation of cells and
tissues, its use covers the agricultural industry for the development of biofertilizers,
as well as the environmental industry to determine contaminating biosensors and
the remediation of contaminated soils or waters [2, 3]. This describes the characteristics and general properties of carbon-based nanomaterials and some applications,
highlighting their use in health.
2 Types of Carbon-Based Nanostructured Materials:
Structure Analysis
Carbon could be defined as one of the elements that are found in the Earth’s crust in
abundance. Nanostructured carbon materials, also known as “NCMs,” give a variety
of allotropes, some of the ones that can be mentioned: diamond, graphene (GR),
amorphous carbon, carbon nanotubes (CNTs), and carbon dot (CDs) with a considerable number of electrochemical applications. As mentioned earlier, carbon can be
organized into several dimensions, from zero- to three-dimensional nanomaterials,
which will give the material its properties.
2.1 Zero-Dimensional Carbon-Based Nanostructures (0D)
Among these dimensional nanostructures, we can find some carbonaceous graphitic
nanoparticles (NPs), such as carbon-based (CQD) and graphene-based (GQD)
quantum dots (Fig. 1), which have potential applications in the development of new
electronic devices. This carbon nanostructure has gained attention, and because of
that, it is considered an alternative material for developing new semiconductors due to
its enhanced properties. Due to this, researchers have been inspired to employ them
in many things, such as bioimaging, optoelectronic, catalytic, and energy storage
applications [4].

42 V. Esparza-Cordero et al.
Fig. 1 Carbon-based
nanostructures (fullerene
C
) zero dimensional (0D)
60
2.2 One-Dimensional Carbon-Based Nanostructures
One of the principal characteristics of one-dimensional (1D) carbon nanostructures is
that they can be used in different fields, which has led researchers to work in various
applications. The primary study nanomaterials are carbon nanotubes (CNT), used as
the central precursor materials for graphite sheets; the main structure formed by this
precursor is cylindrical. However, some carbon allotropes can present a high lengthto-diameter ratio. Usually, CNTs are tubular; the main property of this nanostructure
is that their carbon atoms can present a hexagonal arrangement in a sheet. CNTs
present some attractive characteristics, such as enhanced mechanical properties that
allow these nanomaterials to develop new construction constituents. Also, CNTs
have recently gained particular attention in developing new electronic device fields
because of their unique electronic properties. CNTs have a high Young’s modulus,
and it has been proved that the rigid molecules that compound these materials are
more resistant than steel; also, CNTs are good conductors of heat and electricity.
Finally, CNTs have been used to construct new sensors with real potential in nanoelectromechanical systems (NEMS). The structures of these materials provide new
functionality or enhance the performance of the newly developed devices [5]. The
synthesis of 1D carbon nanostructures includes nanotubes, nanofibers, and nanowires
(Fig. 2).
2.3 Two-Dimensional (2D) Carbon-Based Nanostructures
Recently, graphene has been increasingly used to synthesize new nanomaterials, and
it is highly abundant, has the characteristic of building block of natural graphite, and is
also a good electricity conductor. This new material has excellent conductive, optical
properties like carbon nanotubes. It also has some other specific characteristics;
the main structure is a 2D atomic sheet-like structure, which allows graphene to

Carbon-Based Nanostructured Materials: Designing … 43
Fig. 2 One-dimensional
(1D) carbon-based
nanostructures (carbon
nanotubes)
Fig. 3 Two-dimensional
(2D) carbon-based
nanostructures (graphene)
empower various electronic devices via the quantum Hall effect and massless Dirac
fermions. Graphene nanomaterials present some other exceptional characteristics
like a high surface area, which is the result of an ultra-thin or small formed material,
brilliant electrical conductivity, and enhanced mechanical flexibility, among others;
graphene can also be used in the synthesis of new nanocomposites with that can be
used in many electrochemical applications. Due to the atomically thin structure and
consequent quantum confinement effect, 2D nanomaterials have remarkable physical
and chemical properties [1, 5]. The structure of graphene is represented in Fig. 3.
2.4 Three-Dimensional (3D) Carbon-Based Nanostructures
Because of their structural interconnectedness, three-dimensional carbon-based
nanostructures produce hierarchical porous channels with higher electrical conductivity. Additionally, its structural-mechanical stability is superior. Recently, there
has also been increased interest in various sectors regarding three-dimensional structured nanodiamonds (ND) (Fig. 4), which are formed via high-energy conduction

44 V. Esparza-Cordero et al.
Fig. 4 Three-dimensional
(3D) carbon-based
nanostructures
(nanodiamond)
of graphite, typically by explosion. They are primarily made of sp3carbon, and in
practical application, various functional groups have been used to surface-functioned
diamonds. Compared to 2D substrates, 3D structures are much better because they
can offer more “hot spots,” which significantly promote the adsorption of probe
molecules and a more significant specific surface area. Among the synthesized newly
graphene-based 3D spatial SERS nanoparticles, we can find nanoporous structures
and various layers connecting spatial sandwich structures between graphene and
noble metal NPs, and others are examples of structures [1, 6].
3 Synthesis of Carbon-Based Nanostructured Materials
and Applications
3.1 Chemical Vapor Deposition
This method is one of the most common. It consists of the evaporation of the reactive
compounds that can be in a solid or liquid phase and, after being evaporated, can be
deposited in a substrate that can be another material or a layer composed of organic or
inorganic material [7–10]. Chemical vapor deposition (CVP) has a great potential to
produce graphene-based materials with a large area and high quality. The quality and
size of the synthesized materials will depend on the pressure and temperature used
during the process. Also, compared to other methods, CVP uses catalysts and some
precursors instead of solvents. Carbon is almost not soluble in copper, which allows
the deposition of several graphene layers; thus, it is the most used catalyst. Carbon
precursor methane is preferably used because it has been widely studied [9–11].
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