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Y. A. T. Ngandjui et al.

forms a soluble ion pair with the alkaloids. The shifts obtained are particularly large for protons close to the protonated nitrogen, giving additional information on the structure and the stereochemistry of the alkaloid. Another way to obtain resolution of overlapping signals could be the application of the HOHAHA technique, which allows spectra to be obtained from separate spin systems, or the measurement of 2D J-resolved spectra.

The next step in the structure elucidation is the measurement of a 13C-NMR spectrum. Each carbon emits a signal at a shift characteristic of its molecular environment, e.g., aromatic, vinylic, with hetero-atomic substituents. Alkaloids groups have great variety of structure and also many characteristic peaks in 1H-NMR and 13C-NMR spectroscopic. The value of these peak depending of each alkaloids class. For this reason, we will choose and present the general characteristic peak of one class of alkaloids involved in the management of lifestyle diseases.

3.3.4 Characterization ofMonoterpene Indole

Alkaloids

Among alkaloids, monoterpene indole alkaloids were reported to exhibited good activity against lifestyle diseases [143, 144].

3.3.4.1 Qualitative Identication ofMonoterpene Indole Alkaloids

All alkaloids including monoterpene indole alkaloids react with reagents of Dragendorff, Mayer, and Wagner to give colored compounds.

3.3.4.2 Ultra Violet Spectral Properties

The UV spectrum of monoterpene indole alkaloids is carried out in MeOH and the characteristic absorption maxima are around 206–229nm, 276–299nm, 284–324nm, and 367– 392nm [144, 217, 218].

3.3.4.3 Infrared Spectral Properties

The characteristics IR absorptions bands are in the region of 1660–1645cm−1 for the six-member lactam ring, between 1597 and 1731cm−1 for the carbonyl function and at 3145 and 3433cm−1 for hydroxyl groups. In addition, absorptions bands at 3145–3364 cm−1 indicate the existence of N-H groups [144, 217, 219] (Table1).

3.4 Characterization Techniques

ofFlavonoids

In general, phenolic compounds were reported to exhibit good activities against lifestyle diseases. Among phenolic compounds, avonoids were the most reported to possess

such activities [220, 221]. Flavonoids can be identied through both qualitative analysis and spectroscopic methods.

3.4.1 Qualitative Methods

Most avonoids are positive to ferric chloride and Shinoda tests. They can also be positive to test of Molish if they possess sugar moiety in their structures [222].

3.4.2 Spectroscopic Methods

3.4.2.1 UV andIR Characteristic ofFlavonoids

The UV spectrum of avonoids is generally established in polar solvents such as MeOH or EtOH and two absorption maxima are observed, the range from 240 to 290nm (band II) due to ring A and the range from 300 to 400nm (band I) due to ring B, a cynnamoyle system. But these values highly depend on oxygenation pattern of a avonoid (the position and the number of hydroxyl groups) [223, 224] (Scheme 1 and Table2).

IR spectroscopy, compared to the above techniques shows little information, but important information on the elucidation of the structure of avonoids. In fact, most isoavones, hydroxylated avones, chalcones or dihydrochalcones show strong maximum absorption bands between 3200 and 3500 cm−1 due to hydroxyl groups [223]. In addition, an intense absorption band characteristic of the carbonyl group (C=O) of these compounds is observed around 1680cm−1 and is shifted to ca. 1620cm−1 when the C=O is chelated with a hydroxyl. In the IR spectrum of avonoids, an absorption band is also observed around 1600cm−1 due to aromatic double bonds [223, 225].

3.4.2.2 Electronic Impact Mass Spectrometry (EI-MS)

The mass spectrometry data are really necessary in avonoid structure elucidation. This technique is used for the determination of the molecular weight, for the establishment of the distribution of substituents between the A- and B-rings and also for the determination of the site of attachment and the nature of the sugar(s) in C-avonoid and O-glucosides of avonoids. The molecular weight of the basic avonoid nucleus is 222 a.m.u. for the avones, isoavones, and aurones; 224 a.m.u. for the avanones and chalcones; 238 a.m.u. for the avonols, and 240 a.m.u. for the dihydroavonols. From these basic molecular weights, the molecular weights of the elucidated compounds are deduced by adding the 16 mass units (a.m.u) for each -OH and 30 a.m.u. for each -OCH3.

The loss of some molecular ion fragments (M+) is very characteristic of avonoid mass spectra (Scheme 2). The signals displayed accurately indicate the molecular mass per unit

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C-NMR

Isodihydroaminocadambine [144]

H-NMR

C-NMR

64.6 3.09 44.9

22.3

2.68m

22.1 2.97m

27.5

2.45m

27.3 2.05m

120.9 5.33m 120.8

62.7

3.65 dd (12.1, 2.2)

62.4 3.86 dd (12.1, 5.7)

-methyl [143] Razyamide [143] Vincosamide-N-oxide [144]

H-NMR

– – – –

C-NMR

–

H-NMR

–

C-NMR

49.6

–

7.92s

–

3.61m

2.62m

50.3 3.67m

20.3 2.97m

2.65 brs

2.78 d (17.0)

52.7 3.17 dd (11.0, 4.5)

1.98m

33.6 2.45m

2.04 ddd (16.1, 10.3, 9.3)

32.1 2.64 brs

28.6 – 170.7 – 109.2 – 109.4

48.5 – 168.4 7.43s 149.2 7.31s 149.4

5.33 d (11.1)

59.0 5.38 d (1.9) 98.0 5.30 br d (9.5) 98.2

3.14 brs

116.8 1.79 brs 13.1 5.43 dd (10.2, 2.0)

63.9 3.91 d (13.6)

3.69 dd (12.1, 2.1)

C-NMR of some monoterpene indole alkaloids derivatives

6-nor-antirhine-N

H and

Table 1

H-NMR

3.29

1-CH31-NH

–

2 – 137.1 – 133.0 – 137.6 – 134.9

3 4.08 brs 55.6 4.45 d (7.6) 27.7 4.96m 73.9 4.56 brs 55.3

2.92 brs

4 – – – – – – – –

5 3.16 dd (9.0, 4.8)

6 – – 3.03m

7 – 107.5 – 107.8 – 110.2 – 110.4

8 – 128.3 – 126.3 128.6 – 128.8

9 7.38 d (7.8) 118.3 7.45 d (7.6) 118.1 7.38 d (7.6) 118.7 7.28m 118.8

10 6.96 ddd (7.8, 7.8, 1.2) 119.4 7.08 dd (7.6, 7.6) 119.5 7.03 ddd (8.1, 7.3, 1.1) 120.3 6.98t (7.3) 120.3

11 7.01 ddd (7.8, 7.8, 1.2) 121.4– 7.12 dd (7.6,7.6) 121.9 7.11 ddd (8.1, 7.3, 1.1) 122.5 7.07t (7.3) 122.7

12 7.30 d (7.8) 111.8 7.28 d (7.6) 111.1 7.30 d (8.4) 112.4 7.27m 112.5

13 – 137.3 – 136.6 – 134.7 – 137.9

2.10m

14 2.14m

1.55 dd (9.0, 4.2)

2.68m

15 1.66m 32.0 3.82 brs 53.7 2.80m 24.6 2.69m 25.1

16 1.70 ddd (16.8, 9.0, 4.2)

17 2.77m

18 5.05 d (1.8)

5.04 d (1.8)

19 5.69 ddd (18.6, 12.0, 9.6) 140.5 5.38 q (6.8) 121.9 5.63m 134.1 5.51 ddd (9.6, 7.7, 2.9) 134.5

20 2.28 brs 49.6 – 133.0 45.1 4.96 dd (9.5, 2.9) 44.9

3.62 dd (10.8, 6.0)

– – – – 4.62 d (7.8) 100.4 4.57 d (7.8) 100.62′– – – – 3.04t (8.9) 74.2 2.97 dd (13.1, 8.3) 74.43′– – – – 3.27m 77.8 3.33m 78.44′– – – – 3.23m 71.1 3.25m 71.55′– – – – 3.29m 77.9 3.24m 78.16′– – – – 3.89 dd (12.1, 5.4)

21 3.66 dd (10.8, 6.0)

22 – – – – – 169.0 3.31 brs 49.5

1′

– – 3.69s 51.2 – – – –

OCH

NH – – 7.92s – – – –

Band I(300-400)

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Benzoyl

Band II (245-295)

Scheme 1 Absorption bands of the benzoyl and cinnamoyl systems

Table 2 Ultraviolet-visible absorption ranges for avonoids [223]

Band II (nm) Band I (nm) Flavonoid type

230–270 340–390 Chalcones 230–270 380–430 Aurones 245–275 310–330

shoulder Ca. 320 peak Isoavones

250–280 310–350 Flavones 250–280 330–360 Flavonols (3-OH substituted)

250–280 350–385 Flavonols (3-OH free) 270–280 465–560 Anthocyanidins and anthocyanins 275–295 300–330

shoulder

Cinnamoyl

Isoavone

(5-deoxy-6,7-dioxygenated)

oxydation

Flavonones and dihydroavonols

Y. A. T. Ngandjui et al.

charge (m/z) of the fragments they represent. The exact molecular mass for each fragment can be measured to the nearest

0.0001 mass unit if the mass spectrometer is capable of producing a high-resolution mass spectrum. This information allows the precise molecular formula to be calculated from the peak of the molecular ion and the fragment ions [223].

The scheme below highlights the main mechanism of fragmentation [226].

3.4.2.3 1H and13C-NMR

The 1H-NMR spectrum appears important in some cases to distinguish the different subclasses of avonoids such as avanones, avones, and isoavones. From the following table some characteristic proton chemical shifts of avonoids are listed (Table3) [227, 228].

The 1H-NMR spectroscopy also has typical applications:

– In dening the oxygenation pattern of the three rings of

avonoid and in determining the position and number of

methyl groups;

– In distinguishing dihydroavonols, avonones, and

isoavones;

– In determining the number of sugars present and bound to

either α- or β- (by 1H-1H coupling constant);

– In detecting of hydrocarbon side chains such as C-linked

CH3 and C- or O-linked prenyl.

The 13C-NMR help to differentiate the ortho and meta dihydroxyl-substituted B-ring. In fact, when this ring carry

Scheme 2 Fragmentation pathways of avonoids

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Table 3 Approximate proton chemical shifts of avonoids [227]

Chemical shift (ppm) Proton type

ca. 1.7 Prenyl [-CH2-CH=C(CH3)2] methyl groups

(other protons: 3.5 and 5.2ppm) ca. 2.0 Acetate (-OCOCH 2–3 H-3 of avanones (two proton-multiplet)

4.2–6.0 H-2 dihydroavonols: 5.0ppm and H-2 of

avanones: 5–5.5ppm

6.0–8.0 A- and B-ring protons

7.5–8.0 H-2 of isoavones (singlet) 12–14 5-OH (usually observed when

solvent=DMSO-d6)

Table 4 13C-NMR chemical shift ranges of various avonoid carbon types [230]

Usual chemical shift range (ppm)

210–170 Carbonyl (4-keto, acyl)

Aromatic and olenic

150–130 (with ortho/para oxygenation) 165–155 (no ortho/para oxygenation)

125–90 (with ortho/para oxygenation) 135–125 (no ortho/para oxygenation)

Aliphatic

21 (CH2), 122 (CH), 131 (C), 18 (CH3)

55–63 (60–63 for O-disubstituted)

ca. 17–20 C-CH 80–40 (epicatechin C-4, 28ppm) Non oxygenyted (C-2, 3

ca. 100 Methylenedioxy 83–69 (C-1 of O-glycoside, ca.

100ppm)

) and aromatic C-CH

Carbon type

Oxygenated

Non-oxygenated

Isopropenyl [-CH2CH=C(CH3)2]

O-CH

, COCH

avanones)

Oxygenated (sugars)

only hydroxyl, signal is exhibited around 141–149ppm, and in the case of meta substitution, the signal is shifted to low eld around 151–159ppm [223, 229] (Table4).

The 13C-NMR of avonoids has also typical application in

the structure elucidation mainly in:

– Establishing the number of oxygenated carbons on the

avonoid nucleus, the total number of carbon atoms per

molecule and the number of carbons in the sugar moiety; – Identifying of C- and O-linked sugars and; – Determining inter-glycosidic linkage points, acyl substit-

uents, and the site of acylation.

The sugar carbon resonances in the 13C-NMR spectra of

some avonoid C- or O-glycosides are presented in the following table (Table5) [231].

Various avonoid aglycones have also characteristic signals in their 13C-NMR spectra and some of them are given in Table6.

3.5 Characterization Techniques

ofTerpenoids andSteroids

3.5.1 Mass Spectrometry

Several mass techniques can be used for the characterization of terpenoids including IE, FAB-MS, GC-MS, and HPLC-MS.For example, fast atom bombardment spectroscopy (FAB-MS) affords the exact molecular ion peak along with diagnostic fragmentation pattern of the terpenoid molecule. During the recent years, the structure determination of various terpenoids have been done using GC-MS, HPLC-MS spectrometry both coupled with computerized analysis and tandem MS spectral analysis and using FAB-MS in both positive and negative modes [232].

3.5.2 UV andIR Characteristics ofTerpenoids

Functional groups, as olenic bonds (double and triple bonds), carbonyl groups (aldehydes, ketones), carboxylic groups (carboxylic acid and its derivatives) present in terpenoids appear in the UV range between 200 and 350nm. In terpenoid structures, isolated double and triple bonds do not absorb at wavelengths above 200nm, while isolated ketones, aldehydes, carboxylic acids and their derivatives absorb at higher wavelengths with low intensities. Hence, both of the above-mentioned isolated chromophores are not of much value for the structure elucidation. However, UV data become valuable when the conjugated double bonds and α, β-unsaturated carbonyl group are present in the molecule [232].

The IR spectroscopy is commonly used for the identication and structural elucidation of new terpenoids. It gives information about functional groups [232].

3.5.3 Nuclear Magnetic Resonance

Spectroscopy (NMR)

The 1H-NMR and 13C-NMR spectroscopy are the most important tools required for the structure elucidation of terpenoids. The combination of 1D and 2D NMR techniques including COSY, TOCSY, ROESY, 2D-IN ADEQUATE, HMQC, HMBC, HETCOR, and selective INEPT are a great value for the structure elucidation of various terpenoids such as saponins and glycosides consisting of sugar moieties [232]. Terpenoids were greatly implicated in the management of lifestyle diseases. Among these terpenoids, pentacyclic triterpenoids is among the subclass of triterpene which exhibited good activity in the management of lifestyle diseases.

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Table 5 Sugar carbon resonances in the 13C-NMR spectra of some avonoid C- and O-glycosides [231]

Glycoside type C-1 C-2 C-3 C-4 C-5 C-6

O-glucoside (7-linked) 100.2 73.3 76.6 69.8 77.4 60.9 C-glucoside (8-linked) 73.9 71.4 78.8 70.8 81.4 61.5 O-galactoside (3-linked) 102.3 71.3 73.4 68.0 75.8 60.8 C-galactoside (8-linked) 73.9 68.5 75.4 69.1 80.5 61.3 O-α-rhamnoside (3-linked) C-α-rhamnoside (8-linked) O-xyloside (2″-O-linked) O-xyloside (6-linked) 74.6 70.3 78.5 70.0 70.0 – O-α-arabinoside (3-linked) O-α-arabinofuranoside (3-linked) C-α-arabinoside (8-linked) O-glucuronide(3-linked) 101.1 73.7 75.9 71.3 75.9 169.7 O-alloside (3-linked) 99.9 71.6 71.6 67.2 75.1 61.3 O-apioside (2″-O-linked)

101.9 70.4 70.6 71.5 70.1 17.3

77.3 75.0 75.5 72.2 72.2 18.1

102.4 73.7 75.9 69.4 65.5 –

101.8 71.7 70.8 65.9 64.1 –

108.1 82.1 77.2 86.2 61.0 –

74.4 68.2 74.5 68.9 71.0 –

109.0 76.5 79.1 74.0 64.4 –

Y. A. T. Ngandjui et al.

3.5.4 Characterization ofPentacyclic Triterpenoids

3.5.4.1 Qualitative Analysis

Most triterpenoids including pentacyclic triterpenoids respond positively to sulfuric vanillin and Liebermann– Burchard tests.

3.5.4.2 IR Spectroscopy

The Infrared spectrum makes it possible to identify the chemical functions present such as ketones, alcohol, carboxylic acid acetate groups, etc. which characterize most triterpenes [233].

The vibration of the hydroxyl group (OH) is observed between 3200 and 3300 cm−1; the carbonyl group (C=O) band is very intense around 1773 cm−1.The symmetrical deformation vibration of the ester methyl appears between 1370 and 1390 cm−1 and the vibration of the carboxylate group is located between 1100 and 1300cm−1 [234].

3.5.4.3 Mass Spectrometry

In mass spectroscopy, the fragments of the triterpenes are characteristics such as the peaks of fragmentation characteristic of the “Retro-Diels-Alder” mechanism by cleavage of the D/E and C/D cycles of the pentacyclic triterpenes [233]. Indeed, this method is advantageous in the elucidation of structures because it allows the identication of double bonds.

The retro Diels-Alder makes it possible to obtain, for pentacyclic triterpenes possessing a C-12 unsaturation such as urs-12-ene and olean-12-ene, a base peak at m/z=218. When substituents are observed on rings D and E, the value of the base peak may change. There will be a base peak at m/z=203 (218-Me) if there is loss of a methyl group during the elec-

tronic impact or values such as: m/z=248 (203+COOH), m/z=262 (203+COOMe), m/z=265 (203+COOH + OH) and m/z=276 (203+OAc) for substituents of the carboxyl, carbomethoxy, and hydroxyl type remaining on the ion of base peak. These data can, therefore, be used to predict the nature of substitutions on a skeleton [235].

Scheme 3 gives the different probable ions resulting from the Retro Diels-Alder type fragmentation of molecules of the urs-12-ene and olean-12-ene type [236].

Regarding pentacyclic triterpenoids of lupane and hopane types, there is be a base peak that will appear rather towards m/z=189 or m/z=191 when the substitution on the E ring is respectively isopropenyl or isopropyl [235].

3.5.4.4 1H and13C-NMR

The 1H-NMR spectrum of triterpenes is not very explicit. However, it exhibits a series of characteristic peaks in the range 0.5–2ppm, corresponding to the methyl groups. The prole (number, multiplicity, chemical shift) allows orientation regarding the type and series of the triterpene nucleus. The chemical shifts between 2 and 6ppm indicate the presence of functional groups like the hydroxyl, double bond, and carbonyl [233].

The 1H NMR is very characteristic and makes it possible to distinguish certain classes of pentacyclic triterpenes such as ursanes, oleananes, and lupanes. Indeed, the ursane and oleanene derivatives generally exhibit in the 1H-NMR spectrum a large triplet between 5.1 and 5.5ppm of coupling constant J = 3 Hz, characteristic of the vinyl proton H-12. Lup-20(29)-ene derivatives are generally characterized by the presence of two singlets of one proton each between 4.2 and 5.0ppm, characteristic of the 02 vinyl protons on carbon C-29 [237].

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C-1 C-2 C-3 C-4 C-5 C-6

C-NMR data of various avonoid aglycones [223]

Table 6

Flavonoid C-2 C-3 C-4 C-5 C-6 C-7 C-8 C-9 C-10 C-1′ C-2′ C-3′ C-4′ C-5′ C-6′ CH3Flavones

Apigenin 163.8 102.8 181.8 161.9 98.8 164.1 94.0 157.3 103.7 121.3 128.4 116.0 161.5 116.0 128.4

Luteolin 164.5 103.3 182.2 162.1 99.2 164.7 94.2 157.9 104.2 122.1 113.8 146.2 150.1 116.4 119.3

Tricetin 164.2 103.2 181.6 161.6 99.0 164.2 93.9 157.5 104.0 120.9 106.0 146.5 137.9 146.5 106.0

Flavonols

Kampferol 146.8 135.6 175.9 160.7 98.2 163.9 93.5 156.2 103.1 121.7 129.5 115.4 159.2 115.4 129.5

Quercetin 146.9 135.5 175.8 160.7 98.2 163.9 93.3 156.2 103.1 122.1 115.3 145.0 147.6 115.6 120.0

Patuletin 147.1 135.5 176.1 151.8 130.9 157.2 93.7 151.4 103.5 122.1 115.2 145.1 147.8 115.7 120.1 60.3

Isoavones

C-1′ C-2′ C-3′ C-4′ C-5′ C-6′

C=O

C-β C-α

Genistein 153.6 122.4 180.2 162.1 98.6 164.3 93.7 157.5 104.6 121.4 130.0 115.2 157.6 115.2 130.0

Chalcone

Isoliquiritigenin 143.8 117.8 191.4 113.2 164.6 102.6 165.4 107.9 132.3 125.8 130.6 115.8 159.9 115.8 130.6

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Scheme 3 RDA fragmentation of urs-12-ene and olean-12-ene derivatives

Y. A. T. Ngandjui et al.

For C-3 substituted hydroxylated triterpenes, the H-3 proton generally appears between 3.1 and 3.8 ppm while that of acetoxylated triterpenes resonate between 4.2 and

4.6ppm [238].

The methyl signals appearing between 0.5 and 2ppm make it possible to differentiate the types of pentacyclic triterpenes. For example, the unoxidized triterpenes of the olean-12-enes series have 08 singlet signals of 03 protons attributable to 08 angular methyls while those of the urs12- enes series possess 06 singlets of 03 protons and 02 doublets [239].

Regarding the 13C-NMR spectrum, it is more valuable in the structural determination of triterpene nuclei. It allows us to clearly demonstrate the 30 carbon atoms which constitute the backbone of triterpenes, and in particular the carbonyl, carboxylic acid, and alcohol groups, by their specic chemical shifts that is around 210ppm for a carbonyl (C=O), 170 ppm for a carboxylic acid (COOH) and 70ppm for an alcohol (OH) [233].The evolution of this elucidation technique has made it possible to distinguish between the derivatives of the urs-12-ene and olean-12-eneseries. This is made possible by the number of quaternary carbon atoms (which are 05in number for the urs-12-ene series and 06 for the olean-12-ene series) and the chemical shifts of the vinyl carbons C-12 and C-13 resonating respectively at 124.5 ppm (d) and 138 ppm (s) for urs-12-ene while for olean-12-ene the chemical shift of these carbons are at 122.5ppm (d) and

143.5ppm (s) [239].

3.5.5 Characterization Techniques ofSaponins

The structural determination of the aglycones is similar to those of simple terpenoids. However, the 1H-NMR spectrum in the pyridine solvent show the existence of anomeric proton between 4.3 and 6.9 ppm, as well as the

methyl doublets of deoxy-6-hexoses in the range of 1.4–

1.8 ppm. The high values of the coupling constants (J > 7.0 Hz) observed for the anomerous protons of D-glucose, D-galactose, and D-xylose is characteristic for the β-conguration while the small values (J=1–1.5Hz) observed for the L-rhamnose suggest α-conguration. 13CNMR spectrum analysis makes things even easier. The anomeric carbons resonate in a distinct region. This makes it possible to have an estimate of the amount of sugar present in the molecule. The conguration of the L-rhamnose anomere could also be conrmed from the chemical shifts of the C-3 and C-5 carbons. For β-L- rhamnopyranoside these carbons resonate at 75.4 and 73.5 ppm, while for α-L-rhamnopyranoside they are observed at 72.5 and

69.4ppm, respectively [240, 241].

4 Conclusion andOutlooks

Phenolic compounds, alkaloids, terpenoids, and steroids were frequently reported in the literature to exhibit promising activities against lifestyle diseases such as diabetes, cardiovascular diseases, cancers, and chronic respiratory diseases. In this chapter, the main characterization techniques such as infrared (IR), UV-visible, mass spectrometry, and nuclear magnetic resonance (NMR) spectroscopy of these classes of secondary metabolites were discussed. In order to optimize the identication and quantication of these classes of compounds in plants extracts as well as their subsequent isolation, standardized methods using more advanced techniques (metabolomics and molecular networking) needs to be established.

Acknowledgments The authors are grateful to the nancial support of the International Science Programme (ISP), the Natural Products

Chemical Characterization ofPhytoconstituents forLifestyle Diseases

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Research Network for Eastern and Central Africa (NAPRECA) and the University of South Africa (UNISA). The authors are also thankful to the Alexander von Humboldt Foundation (3.4-CMR-Hub) for the nancial support to the Centre of Competence for the Study of Antimicrobial Natural Products from Fungi (CECANAPROF).

References

1. WHO. Noncommunicable diseases. 2022. [cited 2023 Jan 29].

https://Who.int/news- roon/fact- sheets/detail/noncommunicablediseases.

2. Shoaib S.Cardioprotective effect of gemmotherapeutically treated Withania somnifera against chemically induced myocardial. Pak J Bot. 2010;42:1487–99.

3. Jahan N, Khalil-ur-Rahman, Ali S.Cardioprotective and antilipidemic potential of Cyperus rotundus in chemically induced cardiotoxicity. Int J Agric Biol. 2012;14:989–92.

4. Shaito A, Thuan DTB, Phu HT, Nguyen THD, Hassan H, Halabi S, Abdelhaby S, Nasrallah GK, Eid AH, Pintus G.Herbal medicine for cardiovascular diseases: efcacy, mechanisms, and safety. Front Pharmacol. 2020;11:422.

5. Panda VS, Suresh RN.Evaluation of cardioprotective activity of Ginkgo biloba and Ocimum sanctum in rodents. Altern Med Rev. 2009;14:161–71.

6. Kim KH, Moon E, Kang KS, Kim SY, Choi SU, Lee KR.Alkaloids from Acorus gramineus rhizomes and their biological activity. J Braz Chem Soc. 2015;26:3–8.

7. Donnelly LE, Newton R, Kennedy GE, Fenwick PS, Leung RH, Ito K, Russell RE, Barnes PJ.Anti-inammatory effects of resveratrol in lung epithelial cells: molecular mechanisms. Am J Physiol Lung Cell Mol Physiol. 2004;287:L774–83.

8. Islam SU, Ahmed MB, Ahsan H, Lee YS.Recent molecular mechanisms and benecial effects of phytochemicals and plant-based whole foods in reducing LDL-C and preventing cardiovascular disease. Antioxidants. 2021;10(5):784.

9. American Diabetes Association (ADA). Diagnosis and classication of diabetes mellitus. Diabetes Care. 2009;32:S62–7.

10. Malviya N.Antidiabetic potential of medicinal plants. Acta Pol Pharm. 2010;67:113–8.

11. Aggarwal N, Aggarwal S. A review of recent investigations on medicinal herbs possessing antidiabetic properties. J Nutr Disorder Ther. 2011;1:102.

12. Kayarohanam S, Kavimani S.Current trends of plants having antidiabetic activity: a review. J Bioanal Biomed. 2015;7:55–65.

13. Chopra RN, Nayar SL, Chopra IC.Glossary of Indian medicinal plants. New Delhi: Council of Scientic and Industrial Research;

1996.

14. Costantino L, Laura R, Renato P, Tiziana B, Pompeo P, Fabio G.Isolation and pharmacological activities of the Tecoma stans alkaloids. Il Farmaco. 2003;9:781–5.

15. Kiyoteru T, Shinichi T, Junichi K, Shunivhi Y, Kazuo L, Kinzo W, Samisoni J, Taraiasi V, Bill A.Hyperglycemia inhibitor and method for producing the same. Japanese Patent JP 2005132837;

2005.

16. Chattopadhyay RR. A comparative evaluation of some blood sugar lowering agents of plant origin. J Ethnopharmacol. 1999;67:367–72.

17. Luo J, Fort DM, Carlson TJ, Noamesi BK, nii-Amon-Kotei D, King SR, Tsai J, Quan J, Hobensack C, Lapresca P, Waldeck N, Mendez CD, Jolad SD, Bierer DE, Reaven GM. Cryptolepsis san- guinolenta: an ethnobotanical approach to drug discovery and the isolation of a potentially useful new antihyperglycaemic agent. Diabet Med. 1998;15:367–74.

18. Sharma R, Amin H, Galib, Prajapati PK.Antidiabetic claims of Tinospora cordifolia (Willd.) Miers: critical appraisal and role in therapy. Asian Pac J Trop Biomed. 2015;5:68–78.

19. Khanal P, Patil BM, Mandar BK, Dey YN, Duyu T. Network pharmacology-based assessment to elucidate the molecular mechanism of anti-diabetic action of Tinospora cordifolia. Clin Phytosci. 2019;5:1–9.

20. Shimoda H, Nishida N, Ninomiya K, Matsuda H, Yoshikawa M, Javaberine A. New TNF-alpha and nitric oxide production inhibitor, from the roots of Talinum paniculatum. Heterocycles. 2001;55(11):2043–50.

21. Catthareeya T, Papirom P, Chanlun S, Kupittayanant S. Talinum paniculatum (jacq.) gertn: a medicinal plant with potential estrogenic activity in ovariectomized rats. Int J Pharm Pharm Sci. 2013;5(2):478–85.

22. Attia ES, Amer AH, Hasanein MA.The hypoglycemic and antioxidant activities of garden Cress (Lepidium sativum, L.) seed on alloxan-induced diabetic male rats. Nat Prod Res. 2019;33:901–5.

23. Dineshkumar B, Mitra A, Mahadevappa M. Antidiabetic and hypolipidemic effects of mahanimbine (carbazole alkaloid) from Murraya koenigii (Rutaceae) leaves. Int J Phytomed. 2010;2:22–30.

24. Tabopda TK, Ngoupayo J, Liu J, Mitaine-Offer AC, Tanoli SA, Khan SN, Ali MS, Ngadjui BT, Tsamo E, LacailleDubois MA, Luu B. Bioactive aristolactams from Piper umbellatum. Phytochemistry. 2008;69:1726–31.

25. Shibano M, Tsukamoto D, Masuda A, Tanaka Y, Kusano G.Two new pyrrolidine alkaloids, radicamines A and B, as inhibitors of alpha-glucosidase from Lobelia chinensis Lour. Chem Pharm Bull. 2001;49(10):1362.

26. Hammouda Y, Amer MS. Antidiabetic effect of tecomine and tecostanine. J Pharm Sci. 2006;55:1452–4.

27. Jung M, Park M, Lee HC, Kang YH, Kang ES, Kim SK.Antidiabetic agents from medicinal plants. Curr Med Chem. 2006;13:1203–8.

28. Perron NR, Brumaghim JL.A review of the antioxidant mechanisms of polyphenol compounds related to iron binding. Cell Biochem Biophys. 2009;53(2):75–100.

29. Li AN, Li S, Zhang YJ, Xu XR, Chen YM, Li HB.Resources and biological activities of natural polyphenols. Nutrients. 2014;6(12):6020–47.

30. Mojzer BE, Knez Hrnčič M, Škerget M, Knez Ž, Bren U. Polyphenols: extraction methods, antioxidative action, bioavailability and anticarcinogenic effects. Molecules (Basel, Switzerland). 2016;21(7):901.

31. Arranz S, Chiva-Blanch G, Valderas-Martínez P, Medina-Remón A, Lamuela-Raventós RM, Estruch R.Wine, beer, alcohol and polyphenols on cardiovascular disease and cancer. Nutrients. 2012;4(7):759–81.

32. Akkarachiyasit S, Piyawan C, Sirintorn Y, Sirichai A.Inhibitory activities of cyanidin and its glycosides and synergistic effect with acarbose against intestinal -glucosidase and pancreatic—amylase. Int J Mol Sci. 2010;11(9):3387–96.

33. Chakravarthy BK, Gupta S, Gambhir SS, Gode KD.The prophylactic action of (-)-epicatechin against alloxan induced diabetes in rats. Life Sci. 1981;29:2043–7.

34. Li T, Liu J, Zhang X, Ji G. Antidiabetic activity of lipophilic (-)-epigallocatechin-3-gallate derivative under its role of alpha- glucosidase inhibition. Biomed Pharmacother. 2007;61: 91–6.

35. Bhandari MR, Anurakkun NJ, Hong G, Kawabata J. α-Glucosidase and -amylase inhibitory activities of Nepalese medicinal herb Pakhanbhed (Bergenia ciliata, Haw.). Food Chem. 2008;106:247–52.

36. Jung UJ, Lee MK, Jeong KS, Choi MS. The hypoglycaemic effects of hesperidin and naringin are partly mediated by hepatic

https://t.me/medicina_free

Y. A. T. Ngandjui et al.

glucose regulating enzymes in C57BL/KsJ-db/db mice. J Nutr. 2004;134:2499–503.

37. Tchamgoue J, Hazur RM, Tchouankeu JC, Kouam SF, Adhikari A, Hameed A, Green IR, Choudhary MI.Flavonoids and other constituents with insulin secretion activity from Pseudarthria hookeri. Phytochem Lett. 2016;17:181–6.

38. Choi JS, Suh SS, Young HS, Park HJ.Hypolipemic and hypoglycaemic activities of Prunus davidiana in high fat-fed rats. Arch Pharm Res. 1991;1:44–7.

39. Zang Y, Sato H, Igarashi K.Anti-diabetic effects of a Kaempferol glycoside-rich fraction from unripe soybean (Edamame, Glycine max L.Merrill. “Jindai”) leaves on KK-A mice. Biosci Biotechnol Biochem. 2011;75:1–8.

40. Manickam M, Ramanathan M, Jahromi MA, Chansouria JP, Ray AB.Antihyperglycaemic activity of phenolics from Pterocarpus marsupium. J Nat Prod. 1997;60:609–10.

41. Chen YG, Li P, Li P, Yan R, Zhang XQ, Wang Y, Zhang XT, Ye WC, Zhang QW. α-Glucosidase inhibitory effect and simultaneous quantication of three major avonoid glycosides in Microctis folium yan-gan chen. Molecules. 2013;18:4221–32.

42. Ohnishi M, Matuo T, Tsuno T, Hosoda A, Nomura E, Taniguchi H, Sasaki H, Morishita H.Antioxidant activity and hypoglycaemic effect of ferulic acid in STZ-induced diabetic mice and KK-Ay mice. Biofactors. 2004;21:315–9.

43. Basnet P, Kadota S, Shimizu M, Takata Y, Kobayashi M, Namba T.Bellidifolin stimulates glucose uptake in rat 1 broblasts and ameliorates hyperglycaemia in streptozotocin (STZ)-induced diabetic rats. Planta Med. 1995;61:402–5.

44. Rao AV, Gurnkel DM. The bioactivity of saponins: triterpenoid and steroidal glycosides. Drug Metabol Drug Interact. 2000;17:211–35.

45. Ragasa CY, Tsai P, Shen CC.Terpenoids and sterols from the endemic and endangered Philippine trees, Ficus pseudopalma and Ficus ulmifolia. Philippine J Sci. 2009;138(2):205–9.

46. Narender T, Khaliq T, Singh AB, Joshi MD, Mishra P, Chaturvedi JP, Srivastava AK, Maurya R, Agarwal SC.Synthesis of alphaamyrin derivatives and their invivo antihyperglycemic activity. Eur J Med Chem. 2009;44(3):1215–22.

47. Zhang Z, Jiang J, Yu P, Zeng X, Larrick JW, Wang Y.Hypoglycemic and beta cell protective effects of andrographolide analogue for diabetes treatment. J Transl Med. 2009;7:62.

48. Jin L, Shi G, Ning G, Li X, Zhang Z.Andrographolide attenuates tumor necros factor alpha-induced insulin resistance in 3T3-L1 adipocytes. Mol Cell Endocrinol. 2011;332:134–9.

49. Wiedenkeller DE, Sharp GWG. Effects of forskolin on insulin release and cyclic AMP content in rat pancreatic islets. Endocrinology. 1983;113:2311–3.

50. Mbaze LM, Poumale HM, Wansi JD, Lado JA, Khan SN, Iqbal MC, Ngadjui BT, Laatsch H. α-Glucosidase inhibitory pentacyclic triterpenes from the stem bark of Fagara tessmannii (Rutaceae). Phytochemistry. 2007;68(5):591–5.

51. Naik SR, Barbosa FJM, Dhuley JN, Deshmukh V. Probable mechanism of hypoglycemic activity of bassic acid, a natural product isolated from Bumelia sartorum. J Ethnopharmacol. 1991;33(1–2):37–44.

52. Murakami A. Modulation of protein quality control systems by food phytochemicals. J Clin Biochem Nutr. 2013;52(3):215–227.

53. Judy WV, Hari SP, Stogsdill WW, Judy JS, Naguib YM, Passwater R. Antidiabetic activity of a standardized extract (Glucosol) from Lagerstroemia speciosa leaves in Type II diabetics. A dose- dependence study. J Ethnopharmacol. 2003;87(1): 115–7.

54. Sundaram R, Naresh R, Shanthi P, Sachdanandam P. Antihyperglycemic effect of iridoid glucoside, isolated from the leaves of Vitex negundo in streptozotocin-induced diabetic rats with special reference to glycoprotein components. Phytomedicine. 2012;19(3–4):211–6.

55. Yoshikawa M, Yoshizumi S, Ueno T, Matsuda H, Murakami T, Yamahara J, Murakami N.Medicinal foodstuffs. I.Hypoglycaemic constituents from a garnish foodstuff “taranome,” the young shoot of Aralia elata Seem: elatosides G, H, I, J, and K.Chem Pharm Bull. 1995;43:1878–82.

56. Yoshikawa M, Harada E, Murakami T, Matsuda H, Wariishi N, Yamahara J, Murakami N, Kitagawa I. Escins-Ia, Ib, IIa, IIb and IIIa bioactive triterpene oligoglycosides from the seeds of Aesculus hippocastanum L: their inhibitory effects on ethanol absorption, and hypoglycaemic activity on glucose tolerance test. Chem Pharm Bull. 1994;42:1357–9.

57. Hou CC, Lin SJ, Cheng JT, Hsu FL.Antidiabetic dimeric guianolides and a lignan glycoside from Lactuca indica. J Nat Prod. 2003;66:625–9.

58. Fort DM, Ubillas RP, Mendez CD, Jolad SD, Inman WD, Carney JR, Chen JL, Ianiro TT, Hasbun C, Bruening RC, Luo J, Reed MJ, Iwu M, Carlson TJ, King SR, Bierer DE, Cooper R.Novel antihyperglycemic terpenoidquinones from Pycnanthus angolensis. J Org Chem. 2000;65(20):6534–9.

59. Yoshikawa M, Murakami T, Kadoya M, Matsuda H, Muraoka O, Yamahara J, Murakami N, Medicinal foodstuffs. III. Sugar beet. (1): hypoglycaemic oleanolic acid oligoglycosides, betavulgarosides I, II, III, and IV, from the root of Beta vulgaris L. (Chenopodiaceae). Chem Pharm Bull. 1996;44:1212–7.

60. Dubey H, Singh A, Patole AM, Patole AM, Tenpe CR, Ghule BV. Allicin? A SUR2 opener: possible mechanism for the treatment of diabetic hypertension in rats. Rev Bras Farmacogn. 2012;22:1053–9.

61. Gao H, Huang YN, Xu PY, Kawabata J. Inhibitory effect on α-glucosidase by the fruits of Terminalia chebula Retz. Food Chem. 2007;105:628–34.

62. Narender T, Puri A, Shweta, Khaliq T, Saxena R, Bhatia G, Chandra R. 4-hydroxyisoleucine an unusual amino acid as antidyslipidemic and antihyperglycemic agent. Bioorg Med Chem. 2006;16(2):293–6.

63. Alves-Silva JM, Zuzarte M, Marques C, Salgueiro L, Girao H. Protective effects of terpenes on the cardiovascular system: current advances and future perspectives. Curr Med Chem. 2016;23(40):4559–600.

64. Vijayalakshmi A, Ravichandiran V, Velraj M, Hemalatha S, Sudharani G, Jayakumari S. Anti-anaphylactic and antiinammatory activities of a bioactive alkaloid from the root bark of Plumeria acutifolia Poir. Asian Pac J Trop Biomed. 2011;1:401–5.

65. Gao LN, Feng QS, Zhang XF, Wang QS, Cui YL. Tetrandrine suppresses articular inammatory response by inhibiting proinammatory factors via NF-κB inactivation. J Orthop Res. 2016;34:1557–68.

66. Yu Y, Zhang M, Hu Y, Zhao Y, Teng F, Lv X, Li J, Zhang Y, Hatch GM, Chen L. Increased bioavailable berberine protects against myocardial ischemia reperfusion injury through attenuation of NFκB and JNK signaling pathways. Int Heart J. 2018;59:1378–88.

67. Zhao Y, Tian X, Liu G, Wang K, Xie Y, Qiu Y.Berberine protects myocardial cells against anoxia-reoxygenation injury via p38 MAPK-mediated NF-κB signaling pathways. Exp Ther Med. 2019;17:230–6.

68. Li C, Gao Y, Tian J, Shen J, Xing Y, Liu Z.Sophocarpine administration preserves myocardial function from ischemiareperfusion in rats via NF-κB inactivation. J Ethnopharmacol. 2011;135: 620–5.

69. Li J, Li L, Chu H, Sun X, Ge Z.Oral sophocarpine protects rat heart against pressure overload-induced cardiac brosis. Pharm Biol. 2014;52:1045–51.

70. Jiang C, Tong YL, Zhang D, Liu LZ, Wang JF.Sinomenine prevents the development of cardiomyopathy in diabetic rats by inhibiting infammatory responses and blocking activation of NF-κB.Gen Physiol Biophys. 2017;36:65–74.

Chemical Characterization ofPhytoconstituents forLifestyle Diseases

https://t.me/medicina_free

71. Kuchta K, Volk RB, Rauwald HW. Stachydrine in Leonurus cardiaca, Leonurus japonicus, Leonotis leonurus: detection and

quantifcation by instrumental HPTLC and 1H-qNMR analyses. Pharmazie. 2013;68:534.

72. Chen HH, Zhao P, Zhao WX, Tian J, Guo W, Xu M, Zhang C, Lu R. Stachydrine ameliorates pressure overload-induced diastolic heart failure by suppressing myocardial brosis. Am J Transl Res. 2017;9:4250–60.

73. Zhao L, Wu D, Sang M, Xu Y, Liu Z, Wu Q.Stachydrine ameliorates isoproterenol-induced cardiac hypertrophy and brosis by suppressing inammation and oxidative stress through inhibiting NF-κB and JAK/STAT signaling pathways in rats. Int Immunopharmacol. 2017;48:102–9.

74. Meng YY, Liu Y, Hu ZF, Zhang Y, Ni J, Ma ZG, Liao H-H, Wu Q-Q, Tang Q-Z. Sanguinarine attenuates lipopolysaccharideinduced inammation and apoptosis by inhibiting the TLR4/ NF-κB pathway in H9c2 cardiomyocytes. Curr Med Sci. 2018;38:204–11.

75. Yang D, Jia W, Zhu YZ.Leonurine, a potential agent of traditional Chinese medicine: recent updates and future perspectives. Nat Prod Commun. 2016;11:1757–61.

76. Liu XH, Xin H, Hou AJ, Zhu YZ.Protective effects of leonurine in neonatal rat hypoxic cardiomyocytes and rat infarcted heart. Clin Exp Pharmacol Physiol. 2009;36:696–703.

77. Wang R, Li D, Ouyang J, Tian X, Zhao Y, Peng X, Li S, Yu G, Yang J. Leonurine alleviates LPS-induced myocarditis through suppressing the NF-кB signaling pathway. Toxicology. 2019;422:1–13.

78. Hamer M, O’Donovan G, Stamatakis E.High-density lipoprotein cholesterol and mortality: too much of a good thing? Arterioscler Thromb Vasc Biol. 2018;38:669–72.

79. Lin K, Chen H, Chen X, Qian J, Huang S, Huang W.Efcacy of curcumin on aortic atherosclerosis: a systematic review and metaanalysis in mouse studies and insights into possible mechanisms. Oxid Med Cell Longev. 2020;1(520):747.

80. Shahbazi S, Sahrawat TR, Ray M, Dash S, Kar D, Singh S.Drug targets for cardiovascular-safe anti-inammatory: in silico rational drug studies. PLoS One. 2016;11:e0156156.

81. Chen J, Cao X, Cui Y, Zeng G, Chen J, Zhang G.Resveratrol alleviates lysophosphatidylcholine-induced damage and inammation in vascular endothelial cells. Mol Med Rep. 2018;17:4011–8.

82. Cong X, Li Y, Lu N, Dai Y, Zhang H, Zhao X, Liu Y.Resveratrol attenuates the inammatory reaction induced by ischemia/reperfusion in the rat heart. Mol Med Rep. 2014;9:2528–32.

83. Nie J, Zhang L, Zhao G, Du X.Quercetin reduces atherosclerotic lesions by altering the gut microbiota and reducing atherogenic lipid metabolites. J Appl Microbiol. 2019;127:1824–34.

84. Sanchez M, Galisteo M, Vera R, Villar IC, Zarzuelo A, Tamargo J, Perez-Vizcaino F, Duarte J.Quercetin downregulates NADPH oxidase, increases eNOS activity and prevents endothelial dysfunction in spontaneously hypertensive rats. J Hypertens. 2006;24:75–84.

85. Calabriso N, Scoditti E, Massaro M, Pellegrino M, Storelli C, Ingrosso I, Giovinazzo G, Carluccio MA. Multiple antiinammatory and anti-atherosclerotic properties of red wine polyphenolic extracts: differential role of hydroxycinnamic acids, avonols and stilbenes on endothelial inammatory gene expression. Eur J Nutr. 2016;55:477–89.

86. Bhaskar S, Helen A. Quercetin modulates toll-like receptormediated protein kinase signaling pathways in oxLDL-challenged human PBMCs and regulates TLR-activated atherosclerotic inammation in hypercholesterolemic rats. Mol Cell Biochem. 2016;423:53–65.

87. Vera M, Torramade-Moix S, Martin-Rodriguez S, Cases A, Cruzado JM, Rivera J, Escolar G, Palomo M, Diaz-Ricart M. Antioxidant and anti-inammatory strategies based on the

potentiation of glutathione peroxidase activity prevent endothelial dysfunction in chronic kidney disease. Cell Phys Biochem Int J Exp Cell Physiol Biochem Pharmacol. 2018;51:1287–300.

88. Pothineni NVK, Karathanasis SK, Ding Z, Arulandu A, Varughese KI, Mehta JL. LOX-1 in atherosclerosis and myocardial ischemia: biology, genetics, and modulation. J Am Coll Cardiol. 2017;69:2759–68.

89. Ciumarnean L, Milaciu MV, Runcan O, Vesa SC, Rachisan AL, Negrean V, Perné M-G, Donca VI, Alexescu T-G, Para I, Dogaru G.The effects of avonoids in cardiovascular diseases. Molecules. 2020;25(18):4320.

90. Wang P, Sun J, Lv S, Xie T, Wang X.Apigenin alleviates myocardial reperfusion injury in rats by downregulating miR-15b. Med Sci Monit Int Med J Exp Clin Res. 2019;25:2764–76.

91. Xiao C, Xia ML, Wang J, Zhou XR, Lou YY, Tang LH, Zhang FJ, Yang JT, Qian LB.Luteolin attenuates cardiac ischemia/reperfusion injury in diabetic rats by modulating Nrf2 antioxidative function. Oxid Med Cell Longev. 2019;2(719):252.

92. Jia Z, Nallasamy P, Liu D, Shah H, Li JZ, Chitrakar R, Si H, McCormick J, Zhu H, Zhen W, Li Y. Luteolin protects against vascular inammation in mice and TNF-alpha-induced monocyte adhesion to endothelial cells via suppressing IKppaBalpha/ NF-kappaB signaling pathway. J Nutr Biochem. 2015;26:293–302.

93. Ding X, Zheng L, Yang B, Wang X, Ying Y.Luteolin attenuates atherosclerosis via modulating signal transducer and activator of transcription 3-mediated inammatory response. Drug Des Dev Ther. 2019;13:3899–911.

94. Testai L, Piragine E, Piano I, Flori L, Da Pozzo E, Miragliotta V, Pirone A, Citi V, Di Cesare Mannelli L, Brogi S, Carpi S, Martelli A.The citrus avonoid naringenin protects the myocardium from ageing-dependent dysfunction: potential role of SIRT1. Oxid Med Cell Longev. 2020;2019:4650207.

95. Liang JN, Zou X, Fang XH, Xu JD, Xiao Z, Zhu JN, Li H, Yang J, Zeng N, Yuan SJ, Pan R, Fu Y-J, Zhang M, Luo J-F, Wang S, Shan Z-X. The Smad3-miR-29b/miR-29c axis mediates the protective effect of macrophage migration inhibitory factor against cardiac brosis. Biochim Biophys Acta Mol Basis Dis. 2019;1865:2441–50.

96. Devika PT, Prince PSM. (-)-epigallocatechin gallate (EGCG) prevents isoprenaline-induced cardiac toxicity by stabilizing cardiac marker enzymes and membrane-bound ATpases. J Pharm Pharmacol. 2010;60:125–33.

97. Che J, Liang B, Zhang Y, Wang Y, Tang J, Shi G.Kaempferol alleviates ox-LDL-induced apoptosis by up-regulation of autophagy via inhibiting PI3K/Akt/mTOR pathway in human endothelial cells. Cardiovasc Pathol. 2017;31:57–62.

98. Gilbert ER, Liu D.Anti-diabetic functions of soy isoavone genistein: mechanisms underlying effects on pancreatic β-cell function. Food Funct. 2013;4:200–12.

99. Son MJ, Miura Y, Yagasaki K.Mechanisms for antidiabetic effect of gingerol in cultured cells and obese diabetic model mice. Cytotechnology. 2015;67:641–52.

100. Diego A, Marıa F, Yolanda E, Eduardo F, Marcelo A, Ivan P. Natural bioactive compounds as protectors of mitochondrial dysfunction in cardiovascular diseases and aging. Molecules. 2019;24:4259.

101. Panichayupakaranant P, Ahmad MI. Plumbagin and its role in chronic diseases. Adv Exp Med Biol. 2016;929:229–46.

102. Wang SX, Wang J, Shao JB, Tang WN, Zhong JQ. Plumbagin mediates cardioprotection against myocardial ischemia/reperfusion injury through Nrf-2 signaling. Med Sci Monit. 2016;22: 1250–7.

103. Chen C, Liang Z, Chen Q, Li ZG.Irbesartan and emodin on myocardial remodeling in Goldblatt hypertensive rats. J Cardiovasc Pharmacol. 2012;60:375–80.

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