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86) Chemiluminescence and its diagnostic importance.

Chemiluminiscence– the radiation of bodies in the visible range in case of course of chemical reaction. This effect is based that as a result of some chemical interactions energy can be emitted, and it is distinguished in the form of light. It is connected with transitions of electrons between energy levels. On conservation law, an electron, passing from higher energy level to lower shall give the part of the energy equal to a difference of energies of these levels. Return can happen both in the form of allocation of heat, and in the form of emission of quantum of light.

The spontaneous chemiluminescence is divided into three main types:

-mitogenic radiation

-bioluminescence, or exotic luminescence

-superweak luminescence

Mitogenic radiation is an ultra-violet radiation (190-320 nanometers) as which substratum serve proteins and carbohydrates. this radiation stimulates cellular division.

Bioluminescence - the luminescence (420-710 nanometers) inherent in many organisms (bacteria, glowworms, some fishes, mushrooms and protozoa) perceived by an eye. In all cases the bioluminescence is result of enzymatic oxidation of special substances - lyutsiferin which molecules at oxidation are capable to pass into the excited state. The enzyme catalyzing oxidation of lyutsiferin has received the name of a lyutsiferaza.

The superweak luminescence - is a radiation of live organisms, fabrics, cells, their gomogenat and some biosubstrata in visible and infrared area of a range (360-800 nanometers). He can be registered by means of the special receiver of radiation - the photoelectronic multiplier with the photometric installation working in the mode of the counter of photons.

Recently researches have been conducted: luminescences of plasma and serum of blood in the conditions of a stress and in case of various diseases. In case of a stress intensity of a luminescence of plasma of blood increases that specifies strengthening in blood of activity of free radical oxidation. At the same time products of oxidation strengthen destructive processes in cages. inflammatory process in lungs is followed by activization of free radical oxidation and the corresponding increase of level of a luminescence of serum of blood. At the same time intensity of a luminescence depends on degree of expressiveness of inflammatory process. The phase of development of a tumor is characterized by the lowered intensity of a luminescence of blood. Researches on sick people have revealed similar regularity: level of a luminescence of serum of blood of patients with malignant diseases was lowered in comparison with a luminescence of serum of blood of healthy people. At suffering from tuberculosis lungs irrespective of its form the luminescence of serum of blood is raised in comparison with regulation. At patients with cancer of a lung the luminescence of serum of blood was lower, than at healthy faces.

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