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Pulpal Diagnosis
Johnah C. Galicia1 and Asma A. Khan
1
College of Dentistry, Manila Central University, EDSA- Monumento, Caloocan City, Philippines
2
Department of Endodontics, School of Dentistry, UT Health San Antonio, San Antonio, TX, USA
2
Introduction
Over the last few years, there has been a significant shift towards maintaining the dental pulp(1–4). From a research perspective, this push can be viewed as a triumph resulting from years of data gathering to understand the biology of the dental pulp.
To practice a conservative approach in the treatment of pulpitis, an accurate diagnostic method that is quick, non- invasive and quantifiable should be available for chairside use. This test should be able to stage pulpal inflammation accurately, ideally identifying the threshold between pulp tissue that can be saved and tissue that cannot. However, clinicians have had to rely on tests that have been shown over decades to correlate poorly with the histopathology of the pulp(5–9).
The current diagnostic techniques using thermal and electric pulp testers have good sensitivity and specificity in eliciting a positive response on teeth with vital tissues (10, 11). The lingering predicament with these tests is their inability to stage the severity of pulpal disease. In teeth with vital pulp tissue, eliciting a reliable and unambiguous response using these tests is often achieved without too much complexity. The problem lies in determining the extent of inflammation, which these tests cannot decipher. Another concern with the currently used clinical sensibility tests like thermal and electric pulp testing (EPT) is the purpose that these tests were designed for. Rather than measuring blood flow, which is the hallmark of tissue vitality, these tests stimulate nerves. An assumption is made that a positive response to these tests means that vital pulp tissue remains; however, it has been suggested that nerve tissue can remain even after necrosis of the pulp tissue and associated blood vessels(12). In this scenario, a positive response to EPT or cold is a mislead­ing measure of pulp vitality. A more common misleading scenario is of teeth which have recently been traumatized and do not respond to cold or EPT, even though the pulp is vital and a blood supply remains.
Advocates of vital pulp treatment (VPT) and minimally invasive endodontics strongly support a change in diagnostic terminology to better reflect the extent of pulpitis (2). With currently available terminologies, the fate of the dental pulp is categorically dictated by the clinician’s edu­cated interpretation of the patient’s subjective description of the sensation elicited by the tests. Traditionally, the dental pulp can either be kept intact or removed entirely based on the diagnostic
47
Vital Pulp Treatment, First Edition. Edited by Henry F. Duncan and Ikhlas A. El-Karim. © 2024 John Wiley & Sons Ltd. Published 2024 by John Wiley & Sons Ltd.
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terminology emanating from the results of the tests(13). Future efforts should be shifted towards developing non­the extent of pulpal inflammation and towards introducing diagnostic terminologies that favour conservative treatment of the dental pulp.
invasive, biologically- based pulpal diagnostic techniques that accurately measure
DiagnosesofPulpalStatusandTheirAssociated DiagnosticTerminologies
Medical diagnosis is the process of determining which disease or condition explains a person’s symptoms and signs, but for pulpitis, this is not exactly the same. Although it is an inflammatory disease, generally caused by bacterial infection, the diagnosis of pulpitis has remained a conundrum for reasons including the anatomical location of the dental pulp and limitations of current diagnostic tools(14, 15). Over the years, different classifications and diagnostic systems have been advocated for pulpitis, reflecting the challenges and lack of consensus amongst experts (12). Although histology is the gold standard in diagnosis, attempts to classify pulp disease based on histology and symptoms failed due to the lack of correlation(7, 9) and because the pulp must be removed in order to examine it histologically. A more pragmatic approach based on signs and symptoms was introduced, and a different classification was proposed (12). The American Association of Endodontists (AAE) called for a consensus on classification and terminology used for pulp disease that resulted in the current widely used classification(13). This classification describes pulpitis as reversible, indicating that the inflammation should resolve following appropriate management, or irreversible, indicating that the inflamed pulp is considered incapable of healing and for which root canal treatment is indicated. These terms, however, do not take into consideration the current evidence showing that in carious teeth, pulpal inflammation and necrosis may be locally limited to the pulpal tissue adjacent to the carious lesion, while the rest of the pulp remains relatively normal(6). Furthermore, an improved understanding of pulp biology provides evidence of the regenerative capabilities of the pulp. We now know that pulpal inflammation is part of the healing response of the pulp to infectious or noxious stimuli. With the advent of hydraulic calcium silicate cement materials, VPT can be used to predictably save at least part of the pulp in teeth which were preoperatively diagnosed with “irreversible pulpitis”(16, 17).
For these reasons, calls have been made to revise the existing pulpitis terminology and a proposed classification in which the level of pulpitis was graded and described as mild, moderate and severe was introduced(2). This new classification proposed for the first time VPT as an option for treating different stages of pulpits and that root canal treatment can be reserved for cases with advanced pulpal inflammation in which pulpal haemorrhage cannot be controlled during the operative procedure. The classification, however, needs to be validated, and results from recent studies showed that preoperative symptoms could be used to predict the outcome that teeth with moderate pulpits can be successfully treated with partial pulpotomy compared with teeth with severe pulpitis(18) (Table3.1).
TheDiagnosticProcess
Obtaining an accurate diagnosis is essential for the provision of appropriate treatment. The pulpal diagnostic process requires the gathering of information via detailed history taking, followed by thorough clinical examination and application of special investigations. The objective of pulpal
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Table3.1 Current classifications systems ofpulpitis.
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Woltersetal.2017 Hashemetal.2015 AAE2013
The Diagnostic Process 49
Initial pulpitis
Heightened but not lengthened response to the cold test, not sensitive to percussion and no spontaneous pain
Mild pulpitis
Heightened and lengthened reaction to cold, warm and sweet stimuli that can last 20 seconds but then subsides, possibly percussion sensitive
Moderate pulpitis
Clear symptoms, strong, heightened and prolonged reaction to cold, which can last for minutes, possibly percussion sensitive and spontaneous dull pain that can be more or less suppressed with pain medication
Severe pulpitis
Severe spontaneous pain and clear pain reaction to warmth and cold stimuli, often, sharp to dull throbbing pain; patients have trouble sleeping because of the pain (gets worse when lying down). Tooth is very sensitive to touch and percussion
Mild reversible pulpitis
Patients’ descriptions of sensitivity to hot, cold and sweet lasting 15–20 seconds and settling spontaneously
Severe reversible pulpitis
Increased pain for more than several minutes and needing oral analgesics
Irreversible pulpitis
Persistent dull throbbing pain, sharp spontaneous pain and tenderness to percussion or pain exacerbated by lying down
Reversible pulpitis
Discomfort is experienced when a stimulus such as cold or sweet is applied and goes away within a couple of seconds following the removal of the stimulus
Symptomatic irreversible pulpitis
Characteristics may include sharp pain upon thermal stimulus, lingering pain (often 30 seconds stimulus removal), spontaneity (unprovoked pain) and referred pain. Sometimes, the pain may be accentuated by postural changes such as lying down or bending over, and over-
the- counter analgesics are
typically ineffective
Asymptomatic irreversible pulpitis
No clinical symptoms and usually respond normally to thermal testing but may have had trauma or deep caries that would likely result in exposure following removal
diagnosis is to determine the condition of the dental pulp, i.e. not just in an absolute sense (vital versus non- vital), but also the degree of pulp inflammation if present.
Pain History
Obtaining a detailed pain history is an important component of patient assessment and the overall diagnostic process. The mnemonic OPQRST is commonly used in the medical field and can easily be adapted to dental pain as well. In this mnemonic, O stands for onset of event, P stands for pallia­tion/provocation, Q is quality, R is region/radiation, S is severity and T is time when the pain started. Patients who have inflamed pulps may present with a chief complaint of spontaneous pain or one that is only elicited by cold or hot stimuli. Although subjective, the nature, duration and onset of pain are important factors that can help determine whether pulpitis is reversible or irre­versible. Severe pain(19), history of previous pain(20) and presence of spontaneous pain(6, 20) have been considered to be poor prognostic factors and often associated with severe inflammation
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suggestive of irreversible pulpitis. On the other hand, non- spontaneous pain in response to ther­mal changes often indicate reversible pulp inflammation.
Pain can be reported as a symptom in the patient history but can also be elicited as a sign during clinical examination. For example, tenderness to percussion and pain on thermal stimuli can be of diagnostic value. In cases where the patients have severe spontaneous pain, the resulting afferent barrage results in central sensitization (increased responsiveness of neurons of the central nervous system) and referred pain, making diagnosis challenging. This was elegantly demonstrated in a clini­cal study in which patients presenting for endodontic treatment were asked to localize the sympto­matic tooth(21). Patients who did not have percussion sensibility (i.e. those who only had pulpal pain) were less likely to accurately localize the right tooth as compared to those who had percussion sensitivity (30% versus 89.1%). Notably, in patients who did not have percussion sensitivity, the pain intensity was inversely correlated with the patients’ ability to accurately localize the right tooth(21).
Relying on only pain symptoms for diagnosis can be misleading. It should be emphasized that pain is subjective, and pulpitis can be painless in approximately 40% of cases(22), while it was also shown that 40% of cases with salvageable pulps were associated with pain(19). In addition, most patients who presented with a toothache usually have odontogenic pain (and not pain referred to the teeth from a non­can also be perceived by the patient as a toothache. These sources of non­the muscles of mastication, temporomandibular joint, maxillary sinus and cardiac tissues(23–25).
odontogenic source). However, astute clinicians understand that non- odontogenic pain
odontogenic pain include
PulpSensitivity/SensibilityTesting
The patient’s response to pulp sensibility tests plays a critical role in determining the diagnosis and subsequent treatment. However, the lack of standardized methods for performing these tests results in unpredictable results and misinterpretation. Commonly performed sensibility tests are done with thermal (cold and heat) or electrical stimuli. Cold testing is often done using refrigerant spray (1,1,1,2- tetrafluoroethane), and there are many variations in how the test is performed (for example, the size of the cotton pellet, how long the pellet is applied for, the location where the cot­ton pellet is placed, etc.). This noxious cold stimulus is not physiological and elicits a painful response, which is subjectively reported by the patient. The interpretation of the patients’ response by the clinician is neither quantitative nor is objective. There continues to be a lack of a uniform consensus on what constitutes an exacerbated and/or lingering response as compared with a so­called ‘normal’ response. Heat is used less commonly to determine pulpal status, being only used when the chief complaint is that hot foods elicit pain or as a test of last resort. This test is com­monly done after isolation with a dental dam and by rinsing the tooth with hot water or using a rubber wheel to generate heat. Unfortunately, there is a lack of consensus regarding the ideal temperature or duration of the stimulus. Electrical pulp testing (EPT) is another common diagnostic test that is recommended for routine use in combination with thermal tests (26). This non­physiological stimulus is essentially used to determine the responsiveness of the nerves innervating the pulp. It cannot be used to detect the presence or extent of inflammation.
DiagnosticAccuracyofSensibilityTests
The ability of sensitivity tests to accurately determine the vitality of the dental pulp and, to some degree, the pulpal condition has been summarized in a recent systematic review(14). The accuracy of these tests was determined by measuring their sensitivity, specificity and diagnostic odd ratio (DOR) using histology or direct inspection of the pulp as a reference standard. Among the thermal
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Preoperative Diagnosis versus Direct Observation 51
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tests, cold had the highest accuracy, 0.85–0.95(27). The accuracy of the EPT was reported to be in the range of 0.75–0.90(10, 27), and for the heat test, the accuracy was 0.86(28). The result of the review concurs with that of Mainkar and Kim 2018(11), in which, adjusted accuracy of 0.84, 0.82 and 0.72, was reported for cold, EPT and heat test respectively. Using a combination of tests has, however, been shown to improve diagnostic accuracy, with a high DOR reported for combined use of cold and EPT (14), suggesting that both should be used in combination in clinical practice, par­ticularly if there is doubt on the performance of individual tests.
OtherDiagnosticTests
In addition to thermal and electric tests, assessing pulp vitality with pulse oximetry has also been suggested. Pulse oximetry has a reported accuracy of 97%(11, 14) in assessing pulp vitality and is considered a reliable tool; however, to date, there is no pulse oximeter device licensed for dental use to suggest its routine use in practice. The studies using pulse oximetry have modified a medical device which cannot be employed in dental clinics. Another test, the laser doppler flowmeter, offers the advantage of measuring blood flow in the dental pulp as a definitive marker of vitality with an equally high reported accuracy of 97%(11); however, laser doppler is technique sensitive and does not work well if the tooth is heavily restored. While these diagnostics were developed decades ago, they are not used in clinical practice. One reason could potentially be that there has been no significant progress in developing new diagnostic terms that offer a more accurate descrip­tion of pulpal status and the expected results of the sensibility testing.
RadiographicExamination
Although radiographs have limited diagnostic value in pulpitis, they provide vital information that assists in the overall diagnostic and treatment planning process. Radiographic assessment of caries depth as well as clinical indicators of its activity (e.g. symptoms, progression rate and colour), should be used to assist clinical decision­uable tool for the assessment of periapical tissues, pulpal and radicular calcification, root resorp­tion and bone levels. Cone- beam computed tomography has been reported to be helpful in order to identifying pulpitis with more advanced symptomatic pulpitis more likely to show apical rarefac­tion(29); however, the value of this has been questioned as even vital teeth with radiographically visible radiolucencies can heal after VPT(30).
The methods described above can be used to determine with some accuracy if the pulp is vital or necrotic(14) but have limited ability in their capacity to accurately describe the degree of pulpal inflammation and healing potential of the pulp. Therefore, a need exists for the development of new pulp tests based on pulpal disease markers to inform decision- making for VPT(31). In the absence of clinically available molecular tests of inflammation, the colour and intensity of pulp bleeding on exposure may provide a crude measure or surrogate marker of inflammation(32) and capacity to recover after treatment(3, 33), although at present evidence to support arrest of pulpal bleeding affecting treatment outcome in limited(18).
making for VPT. Intraoral periapical radiographs are val-
PreoperativeDiagnosisversusDirectObservation
The recent position statement by the AAE recommends a methodical approach to assessing the pulpal condition directly through visual examination after exposure rather than relying on a preoperative diagnosis to determine the fate of the dental pulp(3). This recommendation is well
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aligned with recent calls to review the current endodontic diagnostic nomenclature and terminology and with a renewed impetus towards maintaining pulp vitality(1, 2, 34, 35). That said, visualization of the pulp may only give limited information that could be of prognostic benefit.
The goal of direct observation is to remove the inflamed or necrotic part of the dental pulp under aseptic conditions. This is followed by an immediate placement of a well- sealing permanent restoration. With this in mind and in lieu of a treatment plan that is a result of a preoperative diagnosis, the dental pulp is managed conservatively. The subjectivity of the preoperative pulpal diagnostic testing results divides pulpitis into ‘reversible’ and ‘irreversible’ and, consequently, its treatment into the removal of the aetiology alone or pulpectomy. However, there is mounting evidence that even with a preoperative diagnosis of irreversible pulpitis, direct visualization of the pulp and performing pulpotomy under optimal conditions can result in favourable outcomes(36).
ClinicaltoMolecularDiagnosticTests:PossibilitiesandChallenges
The dental pulp is an immunocompetent tissue with cytokines, cell surface receptors and other protein markers, which are differentially expressed in inflamed pulps compared with normal pulps(37–43). Gene and protein expression studies support the dynamic immunocompetence of the dental pulp(44–46). A microarray study showed an association between over­expressed genes and pulpal status when the patient was being treated for pulpitis at chairside(44).
Besides being immunocompetent, the dental pulp is not in an isolated environment but rather a reactive tissue that communicates with its surrounding environment. The neural and vascular sup­ply of the endodontium and periodontium are functionally and anatomically connected(47, 48). Therefore, a local neurogenic spread of the inflammatory response can occur from the pulp to the surrounding periodontal tissues(49). Furthermore, there is an overlap in the identified bacterial species in the progression from gingivitis to periodontitis as well as pulpitis to apical periodonti­tis(20). These findings provide a snapshot of the potential use of inflammatory markers to differ­entiate normal from inflamed pulp and to quantify the level of inflammation with appropriate controls and techniques.
Among the substrates that are being explored for diagnostic purposes are the gingival crevicular fluid (GCF) and the dentinal fluid (DF). GCF can be extracted non­through the dentine will be required to collect DF. Further to being invasive, the collection of DF is challenging due to the small volume of analyte available. The biological differences in the GCF and DF between healthy and inflamed, symptomatic dental pulp have been assessed exten­sively in a systematic review by Rechenberg and co­levels of various biomarkers during tooth pain(49, 51, 52). A marked decrease in the levels of these biomarkers was noted after endodontic treatment compared with pre- treatment levels.
The exploratory use of biomarkers such as matrix metalloproteases, substance P, Interleukin- 8, neurokinin- A and others as tools to diagnose not only the vitality of the dental pulp but also to stage the severity of the inflammation has been carried out but is limited in scope(53–57). There has been a distinct lack of commercial drive to explore the possibility of using protein- rich oral fluids as biologically- based diagnostic tools. This is not surprising because of the challenges posed by clinical research, primarily in acquiring participants, the cost and time required to undertake these studies as well as participant drop- outs(58). These challenges are magnified in endodontic clinical studies, especially in biomarker research using oral fluids because of
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