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Pulpal Diagnosis
Johnah C. Galicia1 and Asma A. Khan
1
College of Dentistry, Manila Central University, EDSA- Monumento, Caloocan City, Philippines
2
Department of Endodontics, School of Dentistry, UT Health San Antonio, San Antonio, TX, USA
2
Introduction
Over the last few years, there has been a significant shift towards maintaining the dental pulp(1–4).
From a research perspective, this push can be viewed as a triumph resulting from years of data
gathering to understand the biology of the dental pulp.
To practice a conservative approach in the treatment of pulpitis, an accurate diagnostic method
that is quick, non- invasive and quantifiable should be available for chairside use. This test should
be able to stage pulpal inflammation accurately, ideally identifying the threshold between pulp
tissue that can be saved and tissue that cannot. However, clinicians have had to rely on tests that
have been shown over decades to correlate poorly with the histopathology of the pulp(5–9).
The current diagnostic techniques using thermal and electric pulp testers have good sensitivity
and specificity in eliciting a positive response on teeth with vital tissues (10, 11). The lingering
predicament with these tests is their inability to stage the severity of pulpal disease. In teeth with
vital pulp tissue, eliciting a reliable and unambiguous response using these tests is often achieved
without too much complexity. The problem lies in determining the extent of inflammation, which
these tests cannot decipher. Another concern with the currently used clinical sensibility tests like
thermal and electric pulp testing (EPT) is the purpose that these tests were designed for. Rather
than measuring blood flow, which is the hallmark of tissue vitality, these tests stimulate nerves. An
assumption is made that a positive response to these tests means that vital pulp tissue remains;
however, it has been suggested that nerve tissue can remain even after necrosis of the pulp tissue
and associated blood vessels(12). In this scenario, a positive response to EPT or cold is a misleading measure of pulp vitality. A more common misleading scenario is of teeth which have recently
been traumatized and do not respond to cold or EPT, even though the pulp is vital and a blood
supply remains.
Advocates of vital pulp treatment (VPT) and minimally invasive endodontics strongly support
a change in diagnostic terminology to better reflect the extent of pulpitis (2). With currently
available terminologies, the fate of the dental pulp is categorically dictated by the clinician’s educated interpretation of the patient’s subjective description of the sensation elicited by the tests.
Traditionally, the dental pulp can either be kept intact or removed entirely based on the diagnostic
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Vital Pulp Treatment, First Edition. Edited by Henry F. Duncan and Ikhlas A. El-Karim.
© 2024 John Wiley & Sons Ltd. Published 2024 by John Wiley & Sons Ltd.
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terminology emanating from the results of the tests(13). Future efforts should be shifted towards
developing nonthe extent of pulpal inflammation and towards introducing diagnostic terminologies that favour
conservative treatment of the dental pulp.
invasive, biologically- based pulpal diagnostic techniques that accurately measure
DiagnosesofPulpalStatusandTheirAssociated
DiagnosticTerminologies
Medical diagnosis is the process of determining which disease or condition explains a person’s
symptoms and signs, but for pulpitis, this is not exactly the same. Although it is an inflammatory
disease, generally caused by bacterial infection, the diagnosis of pulpitis has remained a conundrum
for reasons including the anatomical location of the dental pulp and limitations of current
diagnostic tools(14, 15). Over the years, different classifications and diagnostic systems have been
advocated for pulpitis, reflecting the challenges and lack of consensus amongst experts (12).
Although histology is the gold standard in diagnosis, attempts to classify pulp disease based on
histology and symptoms failed due to the lack of correlation(7, 9) and because the pulp must be
removed in order to examine it histologically. A more pragmatic approach based on signs and
symptoms was introduced, and a different classification was proposed (12). The American
Association of Endodontists (AAE) called for a consensus on classification and terminology used
for pulp disease that resulted in the current widely used classification(13). This classification
describes pulpitis as reversible, indicating that the inflammation should resolve following
appropriate management, or irreversible, indicating that the inflamed pulp is considered incapable
of healing and for which root canal treatment is indicated. These terms, however, do not take into
consideration the current evidence showing that in carious teeth, pulpal inflammation and necrosis
may be locally limited to the pulpal tissue adjacent to the carious lesion, while the rest of the pulp
remains relatively normal(6). Furthermore, an improved understanding of pulp biology provides
evidence of the regenerative capabilities of the pulp. We now know that pulpal inflammation is
part of the healing response of the pulp to infectious or noxious stimuli. With the advent of
hydraulic calcium silicate cement materials, VPT can be used to predictably save at least part of the
pulp in teeth which were preoperatively diagnosed with “irreversible pulpitis”(16, 17).
For these reasons, calls have been made to revise the existing pulpitis terminology and a proposed
classification in which the level of pulpitis was graded and described as mild, moderate and severe
was introduced(2). This new classification proposed for the first time VPT as an option for treating
different stages of pulpits and that root canal treatment can be reserved for cases with advanced
pulpal inflammation in which pulpal haemorrhage cannot be controlled during the operative
procedure. The classification, however, needs to be validated, and results from recent studies
showed that preoperative symptoms could be used to predict the outcome that teeth with moderate
pulpits can be successfully treated with partial pulpotomy compared with teeth with severe
pulpitis(18) (Table3.1).
TheDiagnosticProcess
Obtaining an accurate diagnosis is essential for the provision of appropriate treatment. The pulpal
diagnostic process requires the gathering of information via detailed history taking, followed by
thorough clinical examination and application of special investigations. The objective of pulpal
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Table3.1 Current classifications systems ofpulpitis.
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Woltersetal.2017 Hashemetal.2015 AAE2013
The Diagnostic Process 49
Initial pulpitis
Heightened but not lengthened
response to the cold test, not
sensitive to percussion and no
spontaneous pain
Mild pulpitis
Heightened and lengthened
reaction to cold, warm and sweet
stimuli that can last ≤20 seconds
but then subsides, possibly
percussion sensitive
Moderate pulpitis
Clear symptoms, strong,
heightened and prolonged
reaction to cold, which can last for
minutes, possibly percussion
sensitive and spontaneous dull
pain that can be more or less
suppressed with pain medication
Severe pulpitis
Severe spontaneous pain and clear
pain reaction to warmth and cold
stimuli, often, sharp to dull
throbbing pain; patients have
trouble sleeping because of the
pain (gets worse when lying
down). Tooth is very sensitive to
touch and percussion
Mild reversible pulpitis
Patients’ descriptions of
sensitivity to hot, cold and
sweet lasting ≤15–20 seconds
and settling spontaneously
Severe reversible pulpitis
Increased pain for more than
several minutes and needing
oral analgesics
Irreversible pulpitis
Persistent dull throbbing pain,
sharp spontaneous pain and
tenderness to percussion or
pain exacerbated by lying down
Reversible pulpitis
Discomfort is experienced when a
stimulus such as cold or sweet is
applied and goes away within a
couple of seconds following the
removal of the stimulus
Symptomatic irreversible pulpitis
Characteristics may include
sharp pain upon thermal
stimulus, lingering pain (often
≥30 seconds stimulus removal),
spontaneity (unprovoked pain)
and referred pain. Sometimes,
the pain may be accentuated by
postural changes such as lying
down or bending over, and
over-
the- counter analgesics are
typically ineffective
Asymptomatic irreversible pulpitis
No clinical symptoms and usually
respond normally to thermal
testing but may have had trauma
or deep caries that would likely
result in exposure following
removal
diagnosis is to determine the condition of the dental pulp, i.e. not just in an absolute sense (vital
versus non- vital), but also the degree of pulp inflammation if present.
Pain History
Obtaining a detailed pain history is an important component of patient assessment and the overall
diagnostic process. The mnemonic OPQRST is commonly used in the medical field and can easily
be adapted to dental pain as well. In this mnemonic, O stands for onset of event, P stands for palliation/provocation, Q is quality, R is region/radiation, S is severity and T is time when the pain
started. Patients who have inflamed pulps may present with a chief complaint of spontaneous pain
or one that is only elicited by cold or hot stimuli. Although subjective, the nature, duration and
onset of pain are important factors that can help determine whether pulpitis is reversible or irreversible. Severe pain(19), history of previous pain(20) and presence of spontaneous pain(6, 20)
have been considered to be poor prognostic factors and often associated with severe inflammation
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suggestive of irreversible pulpitis. On the other hand, non- spontaneous pain in response to thermal changes often indicate reversible pulp inflammation.
Pain can be reported as a symptom in the patient history but can also be elicited as a sign during
clinical examination. For example, tenderness to percussion and pain on thermal stimuli can be of
diagnostic value. In cases where the patients have severe spontaneous pain, the resulting afferent
barrage results in central sensitization (increased responsiveness of neurons of the central nervous
system) and referred pain, making diagnosis challenging. This was elegantly demonstrated in a clinical study in which patients presenting for endodontic treatment were asked to localize the symptomatic tooth(21). Patients who did not have percussion sensibility (i.e. those who only had pulpal
pain) were less likely to accurately localize the right tooth as compared to those who had percussion
sensitivity (30% versus 89.1%). Notably, in patients who did not have percussion sensitivity, the pain
intensity was inversely correlated with the patients’ ability to accurately localize the right tooth(21).
Relying on only pain symptoms for diagnosis can be misleading. It should be emphasized that pain
is subjective, and pulpitis can be painless in approximately 40% of cases(22), while it was also shown
that 40% of cases with salvageable pulps were associated with pain(19). In addition, most patients
who presented with a toothache usually have odontogenic pain (and not pain referred to the teeth
from a noncan also be perceived by the patient as a toothache. These sources of nonthe muscles of mastication, temporomandibular joint, maxillary sinus and cardiac tissues(23–25).
odontogenic source). However, astute clinicians understand that non- odontogenic pain
odontogenic pain include
PulpSensitivity/SensibilityTesting
The patient’s response to pulp sensibility tests plays a critical role in determining the diagnosis and
subsequent treatment. However, the lack of standardized methods for performing these tests
results in unpredictable results and misinterpretation. Commonly performed sensibility tests are
done with thermal (cold and heat) or electrical stimuli. Cold testing is often done using refrigerant
spray (1,1,1,2- tetrafluoroethane), and there are many variations in how the test is performed (for
example, the size of the cotton pellet, how long the pellet is applied for, the location where the cotton pellet is placed, etc.). This noxious cold stimulus is not physiological and elicits a painful
response, which is subjectively reported by the patient. The interpretation of the patients’ response
by the clinician is neither quantitative nor is objective. There continues to be a lack of a uniform
consensus on what constitutes an exacerbated and/or lingering response as compared with a socalled ‘normal’ response. Heat is used less commonly to determine pulpal status, being only used
when the chief complaint is that hot foods elicit pain or as a test of last resort. This test is commonly done after isolation with a dental dam and by rinsing the tooth with hot water or using a
rubber wheel to generate heat. Unfortunately, there is a lack of consensus regarding the ideal
temperature or duration of the stimulus. Electrical pulp testing (EPT) is another common diagnostic
test that is recommended for routine use in combination with thermal tests (26). This nonphysiological stimulus is essentially used to determine the responsiveness of the nerves innervating
the pulp. It cannot be used to detect the presence or extent of inflammation.
DiagnosticAccuracyofSensibilityTests
The ability of sensitivity tests to accurately determine the vitality of the dental pulp and, to some
degree, the pulpal condition has been summarized in a recent systematic review(14). The accuracy
of these tests was determined by measuring their sensitivity, specificity and diagnostic odd ratio
(DOR) using histology or direct inspection of the pulp as a reference standard. Among the thermal
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Preoperative Diagnosis versus Direct Observation 51
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tests, cold had the highest accuracy, 0.85–0.95(27). The accuracy of the EPT was reported to be in
the range of 0.75–0.90(10, 27), and for the heat test, the accuracy was 0.86(28). The result of the
review concurs with that of Mainkar and Kim 2018(11), in which, adjusted accuracy of 0.84, 0.82
and 0.72, was reported for cold, EPT and heat test respectively. Using a combination of tests has,
however, been shown to improve diagnostic accuracy, with a high DOR reported for combined use
of cold and EPT (14), suggesting that both should be used in combination in clinical practice, particularly if there is doubt on the performance of individual tests.
OtherDiagnosticTests
In addition to thermal and electric tests, assessing pulp vitality with pulse oximetry has also been
suggested. Pulse oximetry has a reported accuracy of 97%(11, 14) in assessing pulp vitality and is
considered a reliable tool; however, to date, there is no pulse oximeter device licensed for dental
use to suggest its routine use in practice. The studies using pulse oximetry have modified a medical
device which cannot be employed in dental clinics. Another test, the laser doppler flowmeter,
offers the advantage of measuring blood flow in the dental pulp as a definitive marker of vitality
with an equally high reported accuracy of 97%(11); however, laser doppler is technique sensitive
and does not work well if the tooth is heavily restored. While these diagnostics were developed
decades ago, they are not used in clinical practice. One reason could potentially be that there has
been no significant progress in developing new diagnostic terms that offer a more accurate description of pulpal status and the expected results of the sensibility testing.
RadiographicExamination
Although radiographs have limited diagnostic value in pulpitis, they provide vital information that
assists in the overall diagnostic and treatment planning process. Radiographic assessment of caries
depth as well as clinical indicators of its activity (e.g. symptoms, progression rate and colour),
should be used to assist clinical decisionuable tool for the assessment of periapical tissues, pulpal and radicular calcification, root resorption and bone levels. Cone- beam computed tomography has been reported to be helpful in order to
identifying pulpitis with more advanced symptomatic pulpitis more likely to show apical rarefaction(29); however, the value of this has been questioned as even vital teeth with radiographically
visible radiolucencies can heal after VPT(30).
The methods described above can be used to determine with some accuracy if the pulp is vital or
necrotic(14) but have limited ability in their capacity to accurately describe the degree of pulpal
inflammation and healing potential of the pulp. Therefore, a need exists for the development of
new pulp tests based on pulpal disease markers to inform decision- making for VPT(31). In the
absence of clinically available molecular tests of inflammation, the colour and intensity of pulp
bleeding on exposure may provide a crude measure or surrogate marker of inflammation(32) and
capacity to recover after treatment(3, 33), although at present evidence to support arrest of pulpal
bleeding affecting treatment outcome in limited(18).
making for VPT. Intraoral periapical radiographs are val-
PreoperativeDiagnosisversusDirectObservation
The recent position statement by the AAE recommends a methodical approach to assessing the
pulpal condition directly through visual examination after exposure rather than relying on a
preoperative diagnosis to determine the fate of the dental pulp(3). This recommendation is well
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Pulpal Diagnosis52
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aligned with recent calls to review the current endodontic diagnostic nomenclature and terminology
and with a renewed impetus towards maintaining pulp vitality(1, 2, 34, 35). That said, visualization
of the pulp may only give limited information that could be of prognostic benefit.
The goal of direct observation is to remove the inflamed or necrotic part of the dental pulp
under aseptic conditions. This is followed by an immediate placement of a well- sealing permanent
restoration. With this in mind and in lieu of a treatment plan that is a result of a preoperative
diagnosis, the dental pulp is managed conservatively. The subjectivity of the preoperative pulpal
diagnostic testing results divides pulpitis into ‘reversible’ and ‘irreversible’ and, consequently, its
treatment into the removal of the aetiology alone or pulpectomy. However, there is mounting
evidence that even with a preoperative diagnosis of irreversible pulpitis, direct visualization of
the pulp and performing pulpotomy under optimal conditions can result in favourable
outcomes(36).
ClinicaltoMolecularDiagnosticTests:PossibilitiesandChallenges
The dental pulp is an immunocompetent tissue with cytokines, cell surface receptors and other
protein markers, which are differentially expressed in inflamed pulps compared with normal
pulps(37–43). Gene and protein expression studies support the dynamic immunocompetence
of the dental pulp(44–46). A microarray study showed an association between overexpressed genes and pulpal status when the patient was being treated for pulpitis at
chairside(44).
Besides being immunocompetent, the dental pulp is not in an isolated environment but rather a
reactive tissue that communicates with its surrounding environment. The neural and vascular supply of the endodontium and periodontium are functionally and anatomically connected(47, 48).
Therefore, a local neurogenic spread of the inflammatory response can occur from the pulp to the
surrounding periodontal tissues(49). Furthermore, there is an overlap in the identified bacterial
species in the progression from gingivitis to periodontitis as well as pulpitis to apical periodontitis(20). These findings provide a snapshot of the potential use of inflammatory markers to differentiate normal from inflamed pulp and to quantify the level of inflammation with appropriate
controls and techniques.
Among the substrates that are being explored for diagnostic purposes are the gingival crevicular
fluid (GCF) and the dentinal fluid (DF). GCF can be extracted nonthrough the dentine will be required to collect DF. Further to being invasive, the collection of DF
is challenging due to the small volume of analyte available. The biological differences in the
GCF and DF between healthy and inflamed, symptomatic dental pulp have been assessed extensively in a systematic review by Rechenberg and colevels of various biomarkers during tooth pain(49, 51, 52). A marked decrease in the levels of these
biomarkers was noted after endodontic treatment compared with pre- treatment levels.
The exploratory use of biomarkers such as matrix metalloproteases, substance P, Interleukin- 8,
neurokinin- A and others as tools to diagnose not only the vitality of the dental pulp but also to
stage the severity of the inflammation has been carried out but is limited in scope(53–57). There
has been a distinct lack of commercial drive to explore the possibility of using protein- rich oral
fluids as biologically- based diagnostic tools. This is not surprising because of the challenges
posed by clinical research, primarily in acquiring participants, the cost and time required to
undertake these studies as well as participant drop- outs(58). These challenges are magnified in
endodontic clinical studies, especially in biomarker research using oral fluids because of
authors (50). The GCF presented increased
invasively, while access
or under-
t.me/Dr_Mouayyad_AlbtousH
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