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82 Santini AH. Intraoral comparison of calcium hydroxide (Calnex) alone and in combination with
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83 Calişkan MK. Success of pulpotomy in the management of hyperplastic pulpitis. Int Endod
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84 Calişkan MK. Pulpotomy of carious vital teeth with periapical involvement. Int Endod
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85 Da Rosa TA. A retrospective evaluation of pulpotomy as an alternative to extraction. Am J Dent.
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86 Asgary S, Ehsani S. Permanent molar pulpotomy with a new endodontic cement: a case series.
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88 Barngkgei IH, Haiboub ES, Alboni RS. Pulpotomy of symptomatic permanent teeth with carious
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94 Taha NA, Abdulkhader SZ. Full pulpotomy with Biodentine in symptomatic young permanent
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9
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Regenerative Endodontics
Matthias Widbiller 1 and Kerstin M. Galler
1
Department of Conservative Dentistry and Periodontology, University Hospital Regensburg, Regensburg, Germany
2
Department of Operative Dentistry and Periodontology, Friedrich- Alexander- University, Erlangen, Germany
2
Introduction
Pulp necrosis can be the result of deep caries, trauma or dental anomalies. As a consequence, bacterial colonization of the root canal triggers apical periodontitis, which may be initially asymp­tomatic (asymptomatic apical periodontitis) or associated with pain or other clinical signs of infection (symptomatic apical periodontitis). Classically, the goal of endodontic treatment is to cure apical periodontitis and resolve the associated clinical symptoms by thorough disinfection and filling of the root canal system.
In this context, root canal treatment of immature teeth poses a particular challenge for clinicians because these teeth typically have short roots with thin dentine walls and a wide apical foramen (Figure9.1a,b). Various treatment options are available, such as apexification with repeated intra­canal dressings with calcium hydroxide or the placement of an apical barrier with a hydraulic calcium silicate cement (HCSC). Traditional apexification involves multiple visits and alternating intracanal applications of calcium hydroxide to induce the formation of a calcified barrier at the root apex(1). Once this barrier is formed, the canal can be obturated with gutta­tooth can be restored. However, several disadvantages come with this treatment that make it no longer the preferred option. Multiple visits are required, therefore the total treatment time is long, and the prolonged contact of root dentine with calcium hydroxide in combination with the lack of a definitive restoration increases the risk of tooth fracture(2, 3). Given these disadvantages, the current recommendation for immature teeth is to place an apical plug with HCSC and seal the rest of the canal with gutta- percha (Figure9.1b). This allows the treatment to be completed quickly and with few appointments (4). However, this will still leave short roots with thin root walls, which means an increased risk of fracture(5, 6).
The vision of endodontic regeneration is based on the restoration of the dentine–pulp complex, where a regenerated pulp tissue will take over all the biological functions of the original pulp, lead­ing to the completion of physiological tooth development.
percha and the
185
Vital Pulp Treatment, First Edition. Editedby Henry F.Duncan andIkhlas A. El-Karim. © 2024John Wiley & Sons Ltd. Published 2024 by John Wiley & Sons Ltd.
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Regenerative Endodontics186
(a) (b) (c)
(a) (b)
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Figure9.1 (a) The apex of a juvenile tooth without completed root growth is very wide, depending on the
stage of development, and has thinly tapering root walls. (b) Root growth is only complete in the first few years after the tooth erupts. When the pulp is lost, the root walls remain thin and the apical foramen wide. (c) The conventional treatment for an immature tooth is to place an apical barrier with HCSC.
BiologicalandMechanicalGoalsofPulpRegeneration
From a biological perspective, regeneration is the process by which tissue is replaced or restored both in form and function(7, 8). Irreversible inflammation or necrosis of the pulp causes clinical and/or radiological symptoms and entails the removal of damaged tissues(9). With the loss of the dental pulp, its various biological abilities are gone (Figure9.2).
One of the most important biological tasks of the dental pulp is root formation and the secretion of dentine during and after tooth development. In addition, the pulp tissue can resist invading microorganisms through mineral deposition and defend itself through specific and non- specific
Figure9.2 Histological representation of the dentine–pulp complex. (a) An odontoblast layer (arrowhead)
can be seen at the interface to the dentine (asterisk). (b) The core of the pulp shows a collagenous extracellular matrix. Primarily fibroblasts and blood vessels with erythrocytes are visible (arrowheads) (H&E staining; scale bars: 100 μm).
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(a)
(b)
Figure9.3 (a) Stages 1–5 of root
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development according to Cvek etal.(3).
Stages 1–4 describe tooth roots during maturation, stage 5 the completed root growth. Source: Adapted from Cvek etal.(3)/ John Wiley & Sons. (b) Stress distribution pattern of an immature tooth during biting shows concentration of mechanical force in the fragile apical area. Source: Adapted from
Bucchi etal.(16). (c) A mature tooth with a
fully developed root can absorb more force and distribute it better. Source: Adapted from
Bucchi etal.(16).
Stage 1
    187
Stage 2 Stage 3 Stage 4 Stage 5
(c)
immune reactions(10, 11). An additional defence mechanism of the dentine–pulp complex is to ensure an outward flow of dentinal fluid through the tubules(12). Consequently, loss of pulp vitality may allow bacteria to migrate more easily through tubular dentine due to the lack of fluid pressure, which is particularly evident in the significant bacterial invasion of dentine in non-
vital teeth(13). Furthermore, the dental pulp is also a sensitive organ that can detect not only thermal, mechanical or chemical stimuli, but also pathogenic irritants. The loss of the pulp inevitably leads to the destruction of neuronal perception, which may play a role in persistent pain conditions and functional changes of the trigeminal nerve(10, 14).
Besides all the biological implications of pulp loss, the long-
term survival of the tooth may be
compromised by the lack of mechanical strength due to possibly incomplete root maturation.(3,
15). The consequences are particularly severe for immature teeth in an early stage of root develop­ment (Figure9.3a). Because of their short roots and thin dentine walls, these teeth are predisposed to fracture(3, 6). Pulp regeneration could facilitate the completion of root development, resulting in increased mechanical strength (Figure9.3b) and, thus, long- term survival of affected teeth(16,
17). Due to the thin and fragile root walls of teeth with pulp necrosis in stages 1–3, endodontic regeneration can offer decisive advantages as growth progresses (Figure9.3c). From stage 4, the root has sufficient stability for both regenerative endodontic approaches and an apical barrier with an HCSC to be suitable for treatment.
HistoricalDevelopmentofRegenerativeEndodontics
The field of regenerative endodontics has developed rapidly in recent years and has attracted con­siderable interest. New biology- based and minimally invasive concepts are increasingly finding their way into everyday clinical practice and are changing the way of thinking in endodontics and
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Regenerative Endodontics188
(a) (b) (c)
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Figure9.4 (a) Preoperative radiograph of a lower second premolar with an open apex and apical
periodontitis. (b) Radiograph confirming the correct placement of MTA over the blood clot. (c) Follow-
radiograph after 18months showing continued root development and resolution of apical radiolucency. Source: Banchs and Trope(20)/Reproduced with permission from Elsevier.
up
conservative dentistry. However, the idea of regenerating tissue inside the root canal system is not new and dates back to the 1960s. In the paper ‘The role of blood clot in endodontic therapy’, researcher Birger Nygaard- Østby studied provoked bleeding in the apical third of root canals obturated with gutta-
percha in the coronal two- thirds (18). He observed partial or complete replacement of the blood clot, mainly by fibrous connective tissue. These findings were forgotten, and attempts to regenerate the pulp were abandoned for several decades before Iwaya etal. made an interesting observation in 2001(19). In a case report, the authors described the treatment of a lower premolar with incomplete root formation, chronic apical periodontitis and a sinus tract. After disinfection and intracanal medication, the authors still demonstrated vital tissue inside the root canal and applied calcium hydroxide only to the area of the canal entrance. Thirty months after treatment, the completion of root formation was radiographically evident and the tooth responded again to electrical pulp testing(19). A landmark clinical report was finally published in 2004 by Banchs and Trope, translating this observation into a potential treatment approach(20). Similar to the case of Iwaya etal., the tooth involved was a lower premolar with an apical radiolu­cency and a sinus tract. The root canal was disinfected and treated with an intracanal medication. After the signs of inflammation had subsided, bleeding into the canal was induced by mechanical irritation of the vital apical tissue (Figure9.4). The resulting blood clot was covered with mineral trioxide aggregate (MTA) at the level of the enamel- cementum junction. After 24months, the osseous lesion had healed, root lengthening and thickening as well as apical closure were clearly visible radiographically(20).
This publication was followed by numerous case reports, case series and clinical studies on this biologically based treatment approach known today as revitalization(21, 22). Increasing scientific evidence and experience with revitalization led to official recommendations from the major endo­dontic societies, the European Society of Endodontology (ESE) and the American Association of Endodontists (AAE), on indications, case selection, procedural details, irrigation, materials and recall(23, 24).
Revitalization
Revitalization has become a well- established treatment alternative to the apical plug after pulpec­tomy or pulp necrosis in immature teeth and is now an integral part of the endodontic treatment spectrum. The aim is to create new tissue inside the root canal so that, if successful, the tooth root
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Revitalization 189
n
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Figure9.5 During revitalization, bleeding into
the root canal is induced by over­A blood clot is formed and covered with a collagen matrix. The root canal is sealed with a bioactive HCSC and the cavity is sealed with an adhesive restoration.
instrumentation.
Blood clot
Collagen
HCSC
Adhesive restoratio
can continue to grow and increase in length and thickness(24). Generally, the revitalization pro­cedure is based on the induction of bleeding into the root canal, whereby mesenchymal stem cells from the periapical tissue are flushed in (Figure9.5). A stable blood clot is formed, which acts as a biological matrix and contains blood-
derived growth factors and multipotent stem cells(25). A key to successful revitalization is optimal infection control and sufficient restoration of the tooth. This allows for the development of a structured connective tissue inside the root canal, which is inner­vated, supplied by blood vessels and has the ability to form new hard tissue(25).
IndicationsandContraindications
Revitalization can now be considered a valid treatment option for immature teeth with pulp necro­sis(24). Particularly in the early stages of root development (stages 1–3) (Figure9.3), when root walls appear thin and fragile, revitalization can offer benefits by continuing root growth. With or without the presence of apical periodontitis, this procedure can allow complete bony healing and progression of root formation. However, revitalization of teeth is not advisable if a dental dam cannot be applied or if extensive coronal hard substance loss would require insertion of an intra­canal post. The indication should be given very cautiously, especially in immature replanted teeth after avulsion, as autonomous revascularization is possible due to the wide- open apical foramen and can be expected in about one- third of cases(26). Recommendations for revitalization of lux­ated teeth cannot be made due to a lack of clinical evidence. Patients with severe general medical impairment (ASA 3 or higher) are also recommended to be treated by conventional approaches(24).
ClinicalProtocol
Revitalization usually takes place in two sessions. During the first session, a local anaesthetic is administered, and the tooth is isolated with a dental dam. The necrotic tissue is removed from the root canal, avoiding mechanical debridement of the canal walls. The length of the immature root
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