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242
A. Hoyos and M. Perez
– Liposuction starts on the deep layer using either Power-X (2018 Solta
Medical–Bausch Health Companies) or MicroAire Systems (MicroAire
Surgical Instruments, LLC, Charlottesville, VA).
– We use 3- or 4-mm straight/curved Mercedes cannula over the lower back and
upper gluteal area through the intergluteal crease access.
– The inner thigh, the lower and lateral gluteal areas are carved using the infra-
gluteal midpoint access, bilaterally, and in distal-to-proximal fashion.
– The upper gluteus maximus line and lower internal must be thoroughly
dened in men, while soft transitions are preferred for women.
– Negative spaces previously marked as triangles are smoothly dened to create
transitions between concavities and convexities over the upper border of the
gluteus maximus, the trochanteric depression, and the medial infragluteal crease.
– Thorough liposuction is performed in the lower ank areas to overlap its de-
nition with the upper portion of the gluteus medius in men, while the immediate supragluteal region should be carefully carved in women, to allow a soft
transition toward the waist.
– The sacral dimples must be carved in all women, since they enhance the
youthful appearance of the gluteal region. Women tend to have greater fat
deposits over this area compared to men.
– Rude transitions over the inner thigh are preferred for the male but avoided for
the female patient. On the lower lateral gluteal region, a negative space allows
us to blend the deep extraction in the lateral thigh and the gluteus to create a
rounded shape.
– The lower midline must be dened at the end depending on the grade of mus-
cular denition.
– Once the posterior zones are completed, liposuction of the inner thigh can be
completed with the patient in the supine position.
TIPS
• Small-diameter cannulas are preferred for the female transition zones.
• The sharper for the squared male, the smoother for the round female.
• No Trochanteric depression for the Female but enhances it in the Male!
• The supragluteal region is critically different between men and women,
sharp for the former, and soft for the latter.

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16.8 Fat Grafting
First of all, we need to differentiate large-volume fat grafting for body contouring
from that of small-volume from face and hand rejuvenation. Although they have just
about the same purpose, the entire process for fat grafting widely differs since the
amount needed to restore the anatomical contour from either of their specic segments could be considered disproportionate. Large-volume lipoinjection aims to
restore the body contour by increasing the size of some body segments that lack
volume or simply need more projection with the use of autologous adipose tissue,
which is why micro-fat grafting or nano-fat grafting would not be suitable techniques.
Lipograft harvesting, processing, and injection techniques have been widely
studied, still there is a lot of controversy in which would be the best process to
achieve the optimal adipose graft. Low-speed centrifugation and low negativepressure aspiration with large-bore cannulas have shown to minimize the adipocyte
damage during fat harvesting, when it comes to separate the fat from any watersoluble solution associated with the fat, however studies show that the adipocytes
are not capable of surviving just by themselves, no matter how purely they are
grafted, which means the recipient area and its dynamics also play important roles
on fat graft survival, and so it would account for the unpredictability of the results.
Buttocks augmentation through fat grafting has increasingly taken over the pre-
vious implant-based tendency, since studies reported an effective and predictable
way to remodel the gluteal region with lipoinjection; however, the procedure is not
awless and mortality due to fat embolism has lately become a concern. It is recommended that small-volume fat injections at the recipient site should be performed
using small-gauge cannulas in a fanning out pattern over multiple sessions, yet this
is not considered an option for body contouring. From the anatomic point of view,
avoiding gluteal vessel damage may prevent those feared complications, yet skilled
surgeons with an accurate anatomical analysis and a systematization of the procedure have also reported cases of fat embolism.
16.8.1 So, What Should WeDo?
Various studies with large case series report that proper patient selection, favorable
instrumentation, patient positioning, proper technique, and knowledge of the local
anatomy are critical to improving the safety and lasting outcomes of large-volume
fat grafting.
We have safely grafted 50–500cc of processed adipose tissue to multiple body
areas including: the deltoids, biceps, triceps, breasts, pectorals, trapezius, latissimus
dorsi, buttocks, quads, calves, and even hamstrings; all over a long learning curve,
which unfortunately includes the multiple complications that we have all suffered from.

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A. Hoyos and M. Perez
A series of studies evaluating the effects of injection techniques on invitro fat
viability and invivo volume retention, in addition to the effects of injection mechanics on material properties of fat demonstrated that either a minimal manipulation
approach or using an adipose tissue injector were superior compared to the modied
Coleman technique. Through the years, we did notice a remarkable increase of fat
retention rates after we implemented some strategies in order to preserve most of
the cellular fraction of the lipoaspirate supernatant (Fig.16.9), by avoiding centrifugation and rather allow its passive decantation within the sterile cannister (High
volume harvesting cannister™– ©2021 Wells-Johnson– Tucson, AZ). These could
be probably explained by the higher concentration of both the cellular vascular stromal fraction (cSVF) and the adipose-derived stem cells (ASC), which are well
known to improve fat survival in clinical reports.
Fig. 16.9 Wells-Johnson
harvesting cannister
(3000cc) with lipoaspirate
after 1-h passive
decantation

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On the other hand, the grafted adipose tissue is also subject to eventual adverse
events after its placement. The majority of them are related to fat necrosis and
require minimal diagnostic or therapeutic intervention, though the larger the graft
volumes the higher the incidence (e.g., fatty necrosis). Likewise, the oncologic
safety of fat grafting has been supported by multiple clinical studies of breast cancer
patients.
As a result, we established a fat grafting protocol for HD2, which could be
summarized as follows
– Use VASER in continuous mode and a mid-power conguration when lipo-
aspirating the thighs and the abdomen.
– Use low-powered liposuction over these areas as well.
– Decantation and minimal manipulation over the fat graft are a must.
– Nano-fat and micro-fat grafting is reserved for small-volume contour decits.
– It is mandatory to follow the postoperative recommendations for long-lasting
results.
Technique for the male
• The subcutaneous plane is preferred for the adipose graft placement: We use a
4-mm basket cannula attached to a MicroAire and peristaltic pump device
(expansion vibration lipolling) to delicately place the graft in the subcutaneous
layers by the infragluteal crease access on each side.
• The superior and middle thirds usually need the most projection.
• The gluteus medius is reached through the intergluteal crease access to place a
selective intramuscular fat graft using a blunt-tip 4-mm 30-degree-curved cannula, attached to a 50-cc syringe lled with processed adipose-graft.
Technique for the female
• We use a 4-mm basket cannula attached to a MicroAire and peristaltic pump
device (expansion vibration lipolling) to delicately place the graft in the subcutaneous layers by the infragluteal crease access on each side. A fanning motion
is performed from deep to supercial.
• The middle third is the one requiring the most projection from the lateral
perspective.
• The trochanteric depression and the superior third may need subcutaneous fat
grafting to achieve a round contour from the posterior perspective.
Warning!
• Do NOT graft the trochanteric depression in the male.
• Always evaluate symmetry with anatomical references and from different
angle views.
• Do NOT graft the gluteus maximus IM plane.

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A. Hoyos and M. Perez
Discipline and strict following of our protocol has allowed us to achieve remark-
able aesthetic outcomes and high satisfaction rates among our patients (Figs.16.10
and 16.11). The entire team at the OR is aware about the procedures and methods
that must be followed to prepare and optimize the adipose graft, which in fact is
subject to a subtle manipulation.
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Fig. 16.10 A 34-year-old female patient who underwent HD2 gluteal liposculpture. A new
improved projection of the buttock is seen in the postoperative photos (d–f) compared to the at
and loose appearance of the preoperative ones (a–c)

16 Buttocks Dynamic Denition (HD2) Liposuction: for Adipose Harvesting...
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abc
def
247
Fig. 16.11 A 36-year-old man who underwent HD2 gluteal liposculpture. Notice the round and
fatty appearance of the preoperative photos (a–c) compared to the new athletic and muscular looking in the postoperative pics (d–f)
16.9 General Postoperative Procedures
• Postoperative garments and foam vests are used since the immediate till
8–12weeks postoperative.
• Patients are enrolled in the postoperative CARE program, which includes daily
lymphatic drainage and massages. Ultrasound therapy is NOT recommended
over fat grafted areas.
• Drains are left at the intergluteal incision and removed after minimal drainage
(approx. 7days postop).
• Although the supine position is recommended during resting, there is no strict
contraindication on positioning, provided that there was no pain. Some patients
actually prefer to use a donut pillow or the prone position while resting.
• Early mobilization is encouraged at 6h post-op.
• Follow-up period should be but not limited to 2years after surgery.

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A. Hoyos and M. Perez
16.10 Conclusions
Our anatomical approach to contouring the gluteal region in men and women has
resulted in excellent aesthetic outcomes with minor complications and no major
complications. The development of our technique was designed to accomplish not
only an ideal muscular and athletic appearance for the masculine buttocks, but also
a slim and round contour for the female.
The step-wise division of the gluteal region into four distinct zones and sub-
zones, for both genders, has improved our understanding of the anatomic regions
that need specic treatments to achieve optimal aesthetic outcomes.
Fat grafting has become the cornerstone of buttock augmentation due to its less
invasive technique with a lower rate of complications compared to those from
implant-based procedures. Although female techniques have been thoroughly studied and multiple approaches have been described, still some challenges exist when
facing some anatomical variations that do not t the regular patient. In contrast, men
gluteal aesthetics are changing the paradigm of what to do, by including new anatomical landmarks that strongly differ from women standards.
Current social tendencies have challenged us plastic surgeons to become even
more critical when analyzing aesthetic standards. As so, patients’ preferences have
changed as the society moves forward to a more inclusive one, where men and
women are not required to dress, behave, and show a stereotype but rather do what
they truly prefer or desire. In that sense, some patients with a masculine phenotype
could request for a soft and rounded feminine shape of their gluteal area (Fig.16.12),
while others with a feminine phenotype could ask for a more muscular and squared
one (Fig.16.13).
Fig. 16.12 A 29-year-old
male patient with a
feminine appearance of his
buttocks

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Fig. 16.13 A 27-year-old
woman with a masculine
appearance of her
gluteal area
249
In comparison, current research on tissue engineering and stem cell cultures is
making great progress in a radical solution to avoid intraoperative graft manipulation, donor site complications, while increasing the long last of the outcomes.
However, the use of stem cell therapies to expand and to grow tissue for reconstruction must evaluate the different variables that imply the execution of an optimal
process. Nonetheless, there is much hope in the evaluation and implementation of
multimodality approaches for autologous fat transplant, including the later inclusion of new methods for fat processing and harvesting that incorporate the preservation of the cSVF and the ASC, which seem to be our “best bet” to guarantee a
long-last fat graft survival whatsoever.

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A. Hoyos and M. Perez
Highlights
• Surgeons should pick the technique for harvesting at which they feel the
most comfortable with.
• VASER technology has the advantage of preserving an optimal cellular
SVF, adipocytes and ASC.
• We do recommend using low-pressure suction when harvesting the low
abdomen and thighs for graft harvesting.
• When large volumes of fat grafting are necessary, use the main collecting
canister to process your adipose graft.
• Use curved cannulas with small syringes and small amounts for fat graft
placement.
• Be aware of the anatomy to avoid vascular injury and contour defects.
• The less you manipulate the graft the better!
• Adherence to key principles in fat grafting techniques should allow a safe
procedure and its continued practice in the setting of increasingly
high demand.
• Tissue engineering could be the answer to avoid donor site complications,
decrease even more the rate of complication rate and why not, prolong
graft survival rates.
• Future of gluteal contour should not be guided by gender approaches but
rather by the patient desires and behavior: female curves could probably t
better to a homosexual man, while sharp muscular buttocks could be preferred in the homosexual female counterpart.
Further Reading
Cárdenas-Camarena L, Arenas-Quintana R, Robles-Cervantes JA.Buttocks fat grafting: 14 years
of evolution and experience. Plast Reconstr Surg. 2011;128(2):545–55. https://doi.org/10.1097/
PRS.0b013e31821b640b.
Chopan M, White JA, Sayadi LR, Buchanan PJ, Katz AJ.Autogenous fat grafting to the breast
and gluteal regions: safety prole including risks and complications. Plast Reconstr Surg.
2019;143(6):1625–32. https://doi.org/10.1097/PRS.0000000000005617.
Condé-Green A, Kotamarti V, Nini KT, etal. Fat grafting for gluteal augmentation: a systematic
review of the literature and meta-analysis. Plast Reconstr Surg. 2016;138(3):437e–46e. https://
doi.org/10.1097/PRS.0000000000002435.
Hoyos AE, Prendergast PM, Hoyos AE, Prendergast PM. Male buttocks and thighs. In: High-
denition body sculpting. Berlin: Springer; 2014. p.137–43.
Hoyos AE, Perez ME, Domínguez-Millán R.Male aesthetics for the gluteal area: anatomy and
algorithm for surgical approach for dynamic denition body contouring. Plast Reconstr Surg.
2020;146(2):284–93. https://doi.org/10.1097/PRS.0000000000007032.

16 Buttocks Dynamic Denition (HD2) Liposuction: for Adipose Harvesting...
Данная книга находится в списке для перевода на русский язык сайта https://meduniver.com/
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Oranges CM, Tremp M, di Summa PG, etal. Gluteal augmentation techniques: a comprehensive
literature review. Aesthet Surg J. 2017;37(5):560–9. https://doi.org/10.1093/asj/sjw240.
Shah B.Complications in gluteal augmentation. Clin Plast Surg. 2018;45(2):179–86. https://doi.
org/10.1016/j.cps.2017.12.001.
Shim YH, Zhang RH.Literature review to optimize the autologous fat transplantation procedure
and recent technologies to improve graft viability and overall outcome: a systematic and retrospective analytic approach. Aesthetic Plast Surg. 2017;41(4):815–31. https://doi.org/10.1007/
s00266- 017- 0793- 3.
Simonacci F, Bertozzi N, Grieco MP, Grignafni E, Raposio E. Procedure, applications, and
outcomes of autologous fat grafting. Ann Med Surg (Lond). 2017;20:49–60. https://doi.
org/10.1016/j.amsu.2017.06.059.
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