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D. Orozco-Rentería et al.
Based on the above we will be able to witness two types of clinical presentations
in the spectrum of fat embolism. One of these will be instaurated within the rst
12h and even in more severe cases in the transoperative period in a subtle way and
with signicant hemodynamic alterations, while the other presentation will be
established progressively between 24–72h after the surgical event with design and
alteration of consciousness. Due to this difference in the presentation of fatty embolism we infer that within the same pathology we face two totally different events but
with one factor in common. Sudden scenarios are usually produced by the passage
into the bloodstream of particles of greater volume than when embolism produce an
obstruction with the consequent decrease in tissue perfusion of the affected organs,
an event known as macroscopic fatty embolism (MAFE), when the presentation is a
product of the biochemical phase of fat micelles that reach the bloodstream we will
be talking about microscopic fat embolism [40].
It is essential that in the presence of a symptomatology such as the one described
above, we make a timely diagnosis or suspect this entity. By not presenting a pathognomonic sign, the diagnosis becomes more complicated, which is why we have
diagnostic scales that will help us identify it in a timely manner [39, 41].
Once the diagnosis is made, it is important to immediately initiate support management for the patient since the specic pharmacological management has been
shown to be ineffective and the mortality is very high in the presence of conrmed
fat embolism [42].
Of the procedures for buttock augmentation mentioned, the one that has attracted
the most attention and has turned on alarm lights is the buttock augmentation with
fat grafting. This was initially due to isolated reports of death in patients who underwent the procedure and subsequently in more extensive studies where a survey of
certied plastic surgeons conrmed by autopsies carried out identied 13 cases of
death from fat embolism, of which 8 were directly associated with gluteal liposuction or lipoinjection [43]. The key nding in these cases was the disruption of a
medium caliber vessel through which macroscopic fatty tissue entering the vena
cava, right heart cavity, and pulmonary parenchyma entered the bloodstream causing the patients to collapse the cardiopulmonary system [44].
This type of situation becomes more relevant due to the determination made by
the National Quality Forum (NQF) in 2002, which established that deaths in ASA-1
patients (most of the plastic surgery patients) are events that “should never occur”
and therefore must be reported immediately [45].
By being identied as a triggering factor for the passage of macroscopic fat tissue into the bloodstream, the laceration of medium-sized vessels of the gluteal
region can conclude that it is a problem derived from the injection technique of the
area where very deep invaded areas try to leave a considerable amount of fatty tissue
at the intramuscular level to grant the best integration given the abundant muscular
irrigation [46]. It should be noted that the recommended way to perform a fat transfer to the gluteal region emphasizes leaving this tissue in a supercial muscular
plane, depositing small amounts in a retrograde direction following a pattern trying
to avoid lesions in the sciatic nerve and ending at the subcutaneous level [47, 48].
Hence, this technique becomes dependent on the skill, experience, and mastery of

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the surgeon, so it becomes difcult to reproduce in all events, and it becomes necessary to standardize the technique to reduce the mortality risks that it entails.
Mortality due to fat transfer to the gluteal region is estimated between 1:2351
and 1:6214 cases [49], a situation that has led experts in the eld to develop research
and modications to techniques that reduce the mortality risk of this procedure.
The rst study carried out in cadavers dedicated to observing the behavior of fat
and determining safety zones with suitable angles for the introduction of the cannula concluded that in an angulation of less than 30° directed from the intergluteal
groove it turned out to have the highest degree of safety and decrease of the risk of
injury of medium-sized vessels as well as of the sciatic nerve, while using the infragluteal groove as access was the site of greatest risk [50, 51] (Fig.9.1).
The rst recommendations issued by the Aesthetic Surgery Education and
Research Foundation (ASERF) consist of 9 points, which will allow us to reduce the
risks of fatty embolism during gluteal lipoinjection. They are:
1. Avoid injecting into the deep muscle.
2. Use >4.1mm diameter single hole injection cannula.
3. Avoid downward angulation of the cannula.
4. Position patient and place incisions to create a path that will avoid deep muscle
injections.
5. Maintain constant 3-dimensional awareness of the cannula tip.
6. Only inject when the cannula is in motion.
7. Consider pulmonary fat embolism in unstable intra-postoperative patients.
8. Review gluteal vascular anatomy.
9. Include the risk of fat embolism and surgical alternatives in the informed consent
process.
Several of the recommendations given may be adapted according to the practice of each surgeon and their experience in this type of procedure; however it is
ab
Fig. 9.1 (a, b) Dissection in cadaver that shows the higher risk to injury blood vessels using inltration through the lower quadrants in this case from the lower lateral. 1 Upper medial quadrant, 2
Upper lateral quadrant, 3 Lower medial quadrant, 4 Lower medial quadrant SGA superior gluteal artery

138
D. Orozco-Rentería et al.
important to avoid at any time the use of Luer Lock cannulas to perform fat
injections by risk of losing the proper plane to deposit fat due to the instability
of the cannula.
The position in which the patient should be placed before starting with the
transfer of fat should guarantee us avoiding entering deep planes where we can
injure medium-sized vessels or the sciatic nerve. Depending on the tastes and the
domain of each surgeon, the appropriate sites to inuence and introduce the fat
are: intergluteal fold, the border of the posterosuperior iliac spine, and the subgluteal groove. Before starting the injection, it is convenient to identify a triangle
where the vertices will correspond to the ischiatic tuberosity, the greater trochanter, and the posterosuperior iliac spine, which contains the most dangerous anatomical structures during the procedure and will serve as a reference to not deepen
with the injection [52].
Depositing the fat in the subcutaneous cellular tissue is the main recommendation with which we will avoid the risk of any vascular lesion and with this a macroscopic fat embolism; however, frequently we will have difculty to deposit the fat
uniformly and without irregularities, which we can decrease or avoid if we previously make a release of the ischiocutaneous ligament [53] with a basketball cannula
that will allow us to distribute the injected fat evenly. In order to perform the injection procedure, it is preferred to use syringes with the prepared fat either by decantation or centrifugation, however it has been proposed to use a power-assisted system
together with its infusion pump for fat injection while still maintaining the safety of
the procedure when deposited at low pressure speeds, with 4mm basketball cannulas that will allow a better separation of the tissues [54, 55]. In cases where due
to the morphological characteristics of the patient, the injection of a large volume of
fat is required to achieve expectations, it is advisable to detach the tissues with a
vibration technique simulating the steps performed with which we will achieve an
adequate tissue expansion that will give us more space to transfer large volumes of
fat without increased tissue pressure and risks of necrosis [56], besides it will be
done quickly when using the infusion pump.
Although the modalities and techniques of fat injection have been modied and
evolved to reduce the risk of a fat embolism that could be fatal for patients, these are
not infallible and the greater the volume injected there will be a greater risk of cause
for vascular lesion that can conclude with the development of a fatty embolism. This
situation has motivated to perform hybrid procedures where the volume of the central gluteal area that is the one that contains the largest amount of vascular structures
is given by the placement of a gluteal implant and ll the external part of the hip
where the implant can be placed with fat, this will allow you to give volume into the
gluteal region with less risk and have more satised patients with their results with
the lowest possible morbidity [57].

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9.14 Conclusion
Buttock surgery has a wide spectrum of complications of which the most feared by
surgeons and serious for patients are macroscopic fatty embolisms since their mortality once it occurs is very high. It is necessary for surgeons who wish to perform
this type of surgery to have a great anatomical knowledge of the gluteal region,
always be attentive to any changes or alterations presented during or after the surgery and, above all, keep in mind the rest of the possible complications, carry out
proper planning of the surgical event, and take all the necessary precautions to
reduce the risks to the maximum.Conict of Interest StatementThe authors declare
no conict of interest or any commercial relationship with any industry or laboratory.
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Part III
Fat Science

Chapter 10
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The Science Behind theFat Graft
GabrielA.Mecott andSheylaGeraldineHernández-Soto
10.1 Introduction
Fat grafting is one of the most useful tools for the plastic surgeon. It can be used to
improve contour, ll defects, and improve the appearance and quality of the skin,
among other uses. In aesthetic surgery, is used to modify the shape and volume of
the desired areas, such as buttocks, breast [1, 2], chest, and abdominal muscles [3].
The fat has been described as having most of the characteristics of the ideal ller,
due to its biocompatibility, versatility, and stability, as well for being long-lasting,
natural-appearing [4] and amenable to be harvested repeatedly [5]. However, it is
not exempt of complications or drawbacks: loss of volume, fat necrosis, and infection are some of the most common problems of fat grafting.
The loss of volume is evidently the most frustrating problem faced with fat grafting. The rst report of loss of volume of the transplanted fat was in 1950, when Peer
reported the loss of 45% of the grafted fat [6]. Nonetheless, the reported loss in
volume after fat grafting usually ranges from 20 to 90% [7–13]. Plastic surgeons are
still struggling to achieve predictable outcomes and high take rates of fat grafts [14].
As stated by Illouz, adipocytes are highly fragile cells with very short lives that once
removed from the body, cannot tolerate excessive manipulation, refrigeration, or
major trauma associated with harvesting [15]. Now, we know that adipocytes are the
most fragile cells among all the cells contained in the grafted fat and they die easily
under ischemic conditions [16].
The rst documented fat autograft was in 1893 by Neuber, in the form of fragments of 1cm obtained from the arm of one patient [17], followed by Lexer [18]
G. A. Mecott (*) · S. G. Hernández-Soto
Plastic and Reconstructive Surgery Division, Faculty of Medicine and University Hospital,
Universidad Autónoma de Nuevo León, Monterrey, Mexico
e-mail: drmecott@nubody.com.mx
© Springer Nature Switzerland AG 2023
D. Del Vecchio, H. Durán (eds.), Aesthetic Surgery of the Buttock,
https://doi.org/10.1007/978-3-031-13802-7_10
145

146
G. A. Mecott and S. G. Hernández-Soto
and Brunings [19]. Most of the initial reports of fat grafting were in the form of
dermofat grafts [6, 20–23]. In 1955, Peer stated, in his cell survival theory, that the
number of viable cells grafted correlates with the ultimate volume of graft survival
[24]. However, the initial techniques of fat harvesting and lack of proper technology
for decreasing cell damage during processing, make difcult to improve viability of
the adipocytes.
Due to the technical difculties and unpredictable results, fat grafting was abandoned for several decades. Renewed interest in autologous fat grafting started in the
late 1970s [25]. With the description of the liposuction by Arpad and Giorgio Fisher
in 1975 [26], the possibility of obtaining fat without large incisions was created.
However, it was until 1977 that liposuction was described as a procedure intended
for grafting purposes [27].
In 1993, Lewis published an interesting article about the frustration that many
surgeons faced those days and how many of them abandoned fat grafting. This article reects the skepticism that prevailed at that time about the utility of fat grafting
for long-term results. He nished his article by suggesting that for those surgeons
who say fat grafting does not work, they soul better say “in my hands” it does not
work [28]. It is amazing how nowadays this advice might still be valid.
Two years later, Coleman published his rst article about fat grafting, trying to
demonstrate the long-term effect of fat grafting as a ller [29]. In this article he
proposed three principles to keep in mind when performing fat grafting:
• Fat is a delicate structure easily damaged by mechanical and chemical insults.
• True injected volume is difcult to judge if too much oil, blood, or lidocaine is
injected.
• Fat is a living tissue that should be placed next to viable tissue that provides
adequate nutritional and respiratory source to survive.
He also advised to not to inject too much fat, because the fat could migrate due
to the excess of pressure and would have necrosis if the graft is not placed next to
viable tissues. Thus, he suggested to perform multiple surgeries instead of risking
for migration or fat necrosis. These recommendations are still valid as of today. We
know that grafting is the process of placing some tissue deprived from its blood supply (in this case adipocytes), in a receptor area that will provide nutrients to the
grafted cells. Then, the whole process of fat grafting should focus on placing viable
adipocytes in the receptor area in a way that these cells receive adequate blood supply in order to assure the survival of most grafted cells.
In recent years, the cell replacement theory was described. This theory proposes
that most adipocytes will die due to ischemia, and will be replaced by new adipocytes during the rst 3months after grafting [30]. Nevertheless, both theories (i.e.,
survival and replacement theories) are not mutually exclusive, since viable preadipocytes should also be injected and placed in viable tissue in order to survive.
The process of fat grafting, as in skin grafts, relies on different variables such as
donor-site election, harvesting, processing, and injection techniques [31]. Each step
involves several variables that might inuence the survival of the graft and will be
discussed in this chapter.

10 The Science Behind theFat Graft
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10.2 Fat Integration andRemodeling
The process of how the adipocytes survive after they are grafted is not well understood. It is evident that there is some bleeding after the fatty graft when it is placed
into the recipient site, initiating release of multiple cytokines and pro inammatory
mediators. This might create an inammatory environment. Then, the adipocytes
will be under ischemic conditions and would survive by nourishing from the vascularized surrounding tissues, by diffusion, until direct capillary vessels are formed [32].
In the rst few weeks after grafting, the volume of the grafts increases slightly as
swelling is observed. Over the next 6weeks, the volume returns to baseline and
stabilize at week 8 [33]. The nal loss of the volume of the fat grafting is the result
of necrosis and ultimately brosis and removal of the death tissue.
In the late 1960s, Sawhney etal. described the behavior of a dermal-fat graft in
porcine models. They grafted pieces of 1.5 cm (per side) between the anterior
abdominal rectus sheath and the rectus muscle. They found that the mass of the fat
decreased in size about 60% by the second week, 80% by the fourth week, and was
almost absent by the eighth week. Most of the fat was replaced with brotic tissue
[21]. It is important to note that they placed the grafts with the dermal side in contact
with the muscle and the fatty portion in contact with the rectus sheath and this might
be the reason for the loss of most of the adipocytes. However, this model explains in
detail the fate of the fat graft when is not well vascularized.
Coban and Ciralik clamped an epigastric ap (rodents) up to 4h and found that
fat necrosis was found at all timepoints, but maximum signs of necrosis were seen
after 3h of ischemia. They also stated that the necrotic volume of fat did not changed
after 3h [34]. Thus, this study implies that 3h might be the maximum time of ischemia that the adipocyte can recover from.
Kato and cols created a model of fat grafting trying to describe the behavior of
the fat graft invivo. In their model, they found that the adipocytes formed three
dened zones after grafting [30] (Fig.10.1).
1. The rst is the surviving (supercial) zone. (100–300μm thick), demarcated at
the end of the rst week. This is the area with viable adipocytes and adjacent to
a vascularized bed.
2. The second zone is the regenerating (intermediate) zone. (600–1200μm thick)
and is seen between the third and fourth week after grafting. In this zone, the
grafted adipocytes die within the rst 24h, but Adipose Stem Cells (ASCs) survive and promote angiogenesis for up to 3days. During the next 3months, new
adipocytes replace the death ones and the tissue is repaired.
3. The nal is the necrosis (central) zone. Here, the adipocytes also die within the
rst 24h, but the remaining ASCs cannot promote revascularization and all cells
nally die by the third day. The result is cicatrization or oil cyst formation.
As mentioned previously, the adipocytes that don’t receive blood supply and
nutrients die within the rst 24h, and they are surrounded by macrophages. This
process causes their volume to be retained for about 4weeks after their death [35].
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