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136
D. Orozco-Rentería et al.
Based on the above we will be able to witness two types of clinical presentations in the spectrum of fat embolism. One of these will be instaurated within the rst 12h and even in more severe cases in the transoperative period in a subtle way and with signicant hemodynamic alterations, while the other presentation will be established progressively between 24–72h after the surgical event with design and alteration of consciousness. Due to this difference in the presentation of fatty embo­lism we infer that within the same pathology we face two totally different events but with one factor in common. Sudden scenarios are usually produced by the passage into the bloodstream of particles of greater volume than when embolism produce an obstruction with the consequent decrease in tissue perfusion of the affected organs, an event known as macroscopic fatty embolism (MAFE), when the presentation is a product of the biochemical phase of fat micelles that reach the bloodstream we will be talking about microscopic fat embolism [40].
It is essential that in the presence of a symptomatology such as the one described above, we make a timely diagnosis or suspect this entity. By not presenting a pathog­nomonic sign, the diagnosis becomes more complicated, which is why we have diagnostic scales that will help us identify it in a timely manner [39, 41].
Once the diagnosis is made, it is important to immediately initiate support man­agement for the patient since the specic pharmacological management has been shown to be ineffective and the mortality is very high in the presence of conrmed fat embolism [42].
Of the procedures for buttock augmentation mentioned, the one that has attracted the most attention and has turned on alarm lights is the buttock augmentation with fat grafting. This was initially due to isolated reports of death in patients who under­went the procedure and subsequently in more extensive studies where a survey of certied plastic surgeons conrmed by autopsies carried out identied 13 cases of death from fat embolism, of which 8 were directly associated with gluteal liposuc­tion or lipoinjection [43]. The key nding in these cases was the disruption of a medium caliber vessel through which macroscopic fatty tissue entering the vena cava, right heart cavity, and pulmonary parenchyma entered the bloodstream caus­ing the patients to collapse the cardiopulmonary system [44].
This type of situation becomes more relevant due to the determination made by the National Quality Forum (NQF) in 2002, which established that deaths in ASA-1 patients (most of the plastic surgery patients) are events that “should never occur” and therefore must be reported immediately [45].
By being identied as a triggering factor for the passage of macroscopic fat tis­sue into the bloodstream, the laceration of medium-sized vessels of the gluteal region can conclude that it is a problem derived from the injection technique of the area where very deep invaded areas try to leave a considerable amount of fatty tissue at the intramuscular level to grant the best integration given the abundant muscular irrigation [46]. It should be noted that the recommended way to perform a fat trans­fer to the gluteal region emphasizes leaving this tissue in a supercial muscular plane, depositing small amounts in a retrograde direction following a pattern trying to avoid lesions in the sciatic nerve and ending at the subcutaneous level [47, 48]. Hence, this technique becomes dependent on the skill, experience, and mastery of
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the surgeon, so it becomes difcult to reproduce in all events, and it becomes neces­sary to standardize the technique to reduce the mortality risks that it entails.
Mortality due to fat transfer to the gluteal region is estimated between 1:2351 and 1:6214 cases [49], a situation that has led experts in the eld to develop research and modications to techniques that reduce the mortality risk of this procedure.
The rst study carried out in cadavers dedicated to observing the behavior of fat and determining safety zones with suitable angles for the introduction of the can­nula concluded that in an angulation of less than 30° directed from the intergluteal groove it turned out to have the highest degree of safety and decrease of the risk of injury of medium-sized vessels as well as of the sciatic nerve, while using the infra­gluteal groove as access was the site of greatest risk [50, 51] (Fig.9.1).
The rst recommendations issued by the Aesthetic Surgery Education and Research Foundation (ASERF) consist of 9 points, which will allow us to reduce the risks of fatty embolism during gluteal lipoinjection. They are:
1. Avoid injecting into the deep muscle.
2. Use >4.1mm diameter single hole injection cannula.
3. Avoid downward angulation of the cannula.
4. Position patient and place incisions to create a path that will avoid deep muscle
injections.
5. Maintain constant 3-dimensional awareness of the cannula tip.
6. Only inject when the cannula is in motion.
7. Consider pulmonary fat embolism in unstable intra-postoperative patients.
8. Review gluteal vascular anatomy.
9. Include the risk of fat embolism and surgical alternatives in the informed consent
process.
Several of the recommendations given may be adapted according to the prac­tice of each surgeon and their experience in this type of procedure; however it is
ab
Fig. 9.1 (a, b) Dissection in cadaver that shows the higher risk to injury blood vessels using inl­tration through the lower quadrants in this case from the lower lateral. 1 Upper medial quadrant, 2 Upper lateral quadrant, 3 Lower medial quadrant, 4 Lower medial quadrant SGA superior glu­teal artery
138
D. Orozco-Rentería et al.
important to avoid at any time the use of Luer Lock cannulas to perform fat injections by risk of losing the proper plane to deposit fat due to the instability of the cannula.
The position in which the patient should be placed before starting with the transfer of fat should guarantee us avoiding entering deep planes where we can injure medium-sized vessels or the sciatic nerve. Depending on the tastes and the domain of each surgeon, the appropriate sites to inuence and introduce the fat are: intergluteal fold, the border of the posterosuperior iliac spine, and the subglu­teal groove. Before starting the injection, it is convenient to identify a triangle where the vertices will correspond to the ischiatic tuberosity, the greater trochan­ter, and the posterosuperior iliac spine, which contains the most dangerous ana­tomical structures during the procedure and will serve as a reference to not deepen with the injection [52].
Depositing the fat in the subcutaneous cellular tissue is the main recommenda­tion with which we will avoid the risk of any vascular lesion and with this a macro­scopic fat embolism; however, frequently we will have difculty to deposit the fat uniformly and without irregularities, which we can decrease or avoid if we previ­ously make a release of the ischiocutaneous ligament [53] with a basketball cannula that will allow us to distribute the injected fat evenly. In order to perform the injec­tion procedure, it is preferred to use syringes with the prepared fat either by decanta­tion or centrifugation, however it has been proposed to use a power-assisted system together with its infusion pump for fat injection while still maintaining the safety of the procedure when deposited at low pressure speeds, with 4mm basketball can­nulas that will allow a better separation of the tissues [54, 55]. In cases where due to the morphological characteristics of the patient, the injection of a large volume of fat is required to achieve expectations, it is advisable to detach the tissues with a vibration technique simulating the steps performed with which we will achieve an adequate tissue expansion that will give us more space to transfer large volumes of fat without increased tissue pressure and risks of necrosis [56], besides it will be done quickly when using the infusion pump.
Although the modalities and techniques of fat injection have been modied and evolved to reduce the risk of a fat embolism that could be fatal for patients, these are not infallible and the greater the volume injected there will be a greater risk of cause for vascular lesion that can conclude with the development of a fatty embolism. This situation has motivated to perform hybrid procedures where the volume of the cen­tral gluteal area that is the one that contains the largest amount of vascular structures is given by the placement of a gluteal implant and ll the external part of the hip where the implant can be placed with fat, this will allow you to give volume into the gluteal region with less risk and have more satised patients with their results with the lowest possible morbidity [57].
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9.14 Conclusion
Buttock surgery has a wide spectrum of complications of which the most feared by surgeons and serious for patients are macroscopic fatty embolisms since their mor­tality once it occurs is very high. It is necessary for surgeons who wish to perform this type of surgery to have a great anatomical knowledge of the gluteal region, always be attentive to any changes or alterations presented during or after the sur­gery and, above all, keep in mind the rest of the possible complications, carry out proper planning of the surgical event, and take all the necessary precautions to reduce the risks to the maximum.Conict of Interest StatementThe authors declare no conict of interest or any commercial relationship with any industry or laboratory.
References
1. ASPS.En línea. 2018. https://www.plasticsurgery.com.
2. Roberts T, Toledo L, Zulmira B.Augmentation of the buttocks by microfat grafting. Aesthet Surg J. 2001;21:311–9.
3. Lee E, Roberts T, Bruner T.Ethnic considerations in buttocks aesthetics. Semin Plast Surg. 2009;23:232–44.
4. Roberts T, Weinfeld A, Bruner T, Nguyen K.Universal and ethnic ideals of beautiful but­tocks are best obtained by autologous micro fat grafting and liposuction. Clin Plast Surg. 2006;33:371–94.
5. Wong W, Motakef S, Lin Y, Gupta S.Redening the ideal buttocks: a population analysis. Plast Reconstr Surg. 2016;137(6):1739–47.
6. Frank S, Flusberg M, Friedman S, Sternschein M, Wolf E, Stein M.Aesthetic surgery of the buttocks: imaging appearance. Skeletal Radiol. 2014;43:133–9.
7. Aboudib J, Serra F, Cardoso C. Gluteal augmentation: technique, indications and implant selection. Plast Reconstr Surg. 2012;130:933–5.
8. Bartles R, O'Maley J, Douglas W. An unusual use of the Cronin breast prosthesis. Plast Reconstr Surg. 1969;44:500.
9. Gonzalez-Ulloa M.Gluteoplasty: a 10-year report. Aesthetic Plast Surg. 1991;15(1):85–91.
10. Robles J, Taglapertra J, Grandl Y. Gluteoplastia de aumento: Implante submuscular. Cir Plástica Iberolatinoamericana. 1984;10:365–75.
11. De la Peña A, Rubio O, Cano J, Cedillo M, Garces M.Subfascial gluteal augmentation. Clin Plast Surg. 2006;33:405–22.
12. Vergara R, Marcos M.Intramuscular gluteal implants. Aesthetic Plast Surg. 1996;20(3):259–62.
13. Serra F, Aboudib J, Visser-Cedrola J, Cardoso C.Gluteoplasty: anatomic basis and technique. Aesthet Surg J. 2010;30:579–92.
14. Currin S, Mirjalili S, Meikle G, Stringer M.Revisiting the surface anatomy of the sciatic nerve in the gluteus maximus muscle and augmentation gluteoplasty. Clin Anat. 2014;1:1–6.
15. Hwang S, Seok-Nam Y, Hwang K, Ho-Han Y.Thickness and tension of the gluteal aponeurosis and the implications for subfascial gluteal augmentation. J Anat. 2012;221:69–72.
140
16. Hwang K, Seok-Nam Y, Ho-Han S, Hwang S.The intramuscular course of the inferior glu­teal nerve in the gluteus maximus muscle and augmentation gluteoplasty. Ann Plast Surg. 2009;63:361–5.
17. Serra F, Aboudib J, Garcia-Marques R.Reducing wound complications in gluteal augmenta­tion surgery. Plast Reconstr Surg. 2012;130:706–13.
18. Bruner T, Roberts T, Nguyen K.Complications of buttocks augmentation: diagnosis, manage­ment and prevention. Clin Plast Surg. 2006;33:449–66.
19. Mendieta C.Gluteoplasty. Aesthet Surg J. 2003;23:441–55.
20. Serra F, Aboudib J. Gluteal implant displacement: diagnosis and treatment. Plast Reconstr Surg. 2014;23:441–55.
21. Senderoff D.Aesthetic surgery of the buttocks using implants: practice-based recommenda­tions. Aesthet Surg J. 2016;36(5):559–76.
22. Senderoff D.Revision buttock implantation: indications, procedures and recommendations. Plast Reconstr Surg. 2017;139:327–35.
23. Harrison D, Selvaggi G.Gluteal augmentation surgery: indications and surgical management. J Plast Reconstr Aesthet Surg. 2007;60:922–8.
24. Vergara R, Amezcua H.Intramuscular gluteal implants: 15 years experience. Aesthet Surg J. 2003;23(2):86–91.
25. Serra F, Aboudib J, Garcia-Marques R. Intramuscular technique for gluteal augmentation: determination and quantication of muscle atrophy and implant position by computed tomog­raphy scan. Plast Reconstr Surg. 2013;131:253–9.
26. Serra F, Aboudib J, Salles-Neto J, Amaral-Cossich V, Pinheiro-Rodrigues N, Fuhro-de Oliveira K, Garcia-Marques R. Volumetric and functional evaluation of the gluteus maxi­mus muscle after augmentation gluteoplasty using silicone implants. Plast Reconstr Surg. 2015;135:533–41.
27. Mod M, Gonzalez R, De la Peña A, Mendieta C, Senderoff D, Jorjani S.Buttock augmenta­tion with silicone implants: a multicenter survey review of 2226 patients. Plast Reconstr Surg. 2013;131:897–901.
28. Kling R, Mehrara B, Pusic A, Young V, Hume K, Crotty C, Rubin J.Trends in autologous fat grafting to the breast. Plast Reconstr Surg. 2013;132:35–46.
29. Palumbo P, Miconi G, Cinque B, Torre C, Lombardi F. In vitro evaluation of different methods of handling human liposuction and adipose derived stem cells. J Cell Physiol. 2015;230(8):1974–81.
30. Gir P, Brown S, Oni G, Kashe N, Mojallal A, Rohrich R. Fat grafting: evidence-based review on autologous fat harvesting, processing, reinjection and storage. Plast Reconstr Surg. 2012;130:249–58.
31. Strong A, Cedena P, Rubin P, Coleman S, Levi B.The current state of fat grafting: a review of harvesting, processing and injection techniques. Plast Reconstr Surg. 2015;136:897–912.
32. Kato H, Mineda K, Eto H. Degeneration, regeneration and cicatrization after fat graft­ing: dynamic total tissue remodeling during the rst 3 months. Plast Reconstr Surg. 2014;133(3):303e–13e.
33. Kang D, Luan J.Fat necrosis after autologous fat transfer (AFT) to breast: comparison of low­speed centrifugation with sedimentation. Aesthetic Plast Surg. 2018;42:1457–64.
34. Del Vecchio D, Del Vecchio S. The graft-to-capacity ratio: volumetric planning in large­volume fat transplantation. Plast Reconstr Surg. 2014;133:561–9.
35. Nathan-Costa A, Melo-Mendes D, Toufen C, Arrunantegui G, Caruso P, Ribero de Carvalho C.Adult respiratory distress syndrome due to fat embolism in the postoperative period follow­ing liposuction and fat grafting. J Bras Pneumol. 2008;34:622–5.
36. Wang H, Zheng J, Deng C, Liu Q, Yang S. Fat embolism syndromes following liposuction. Aesthetic Plast Surg. 2008;32:731–6.
37. El-Ali K, Gourlay T.Assessment of the risk of systemic fat mobilization and fat embolism as a consequence of liposuction: exvivo study. Plast Reconstr Surg. 2006;117:782–4.
D. Orozco-Rentería et al.
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141
38. Miller P, Prahlow J.Autopsy diagnosis of fat embolism syndrome. Am J Forensic Med Pathol. 2011;32:291–9.
39. Kwiatt M, Seamon M.Fat embolism syndrome. Int J Crit Illn Inj Sci. 2013;3:64–8.
40. Duran H, Cardenas-Camarena L, Bayter J, Ramos-Gallardo G, Robles-Cervantes J.Microscopic and macroscopic fat embolism (MIFE and MAFE): solving the puzzle with cases reports. Plast Reconstr Surg. 2018;142:569e–77e.
41. Taviloglu K, Yanar H.Fat embolism syndrome. Surg Today. 2007;37:5–8.
42. Shaikh N, Parchani A, Bhat V, Kattren M.Fat embolism syndrome: clinical and imaging con­siderations: case report and review of literature. Indian J Crit Care Med. 2008;12:32–6.
43. Cardenas-Camarena L, Bayter J, Aguirre-Serrano H, Cuenca-Pardo J.Deaths caused by glu­teal lipoinjection: what are we doing wrong? Plast Reconstr Surg. 2015;136:58–66.
44. Aguirre-Serrano H, Bernal M, Navarro A, Montes G, Morales P, Tellez N.Embolia grasa mac­roscópica por lipoinyección glútea. ¿Una nueva patología? Rev Col Cir Plast. 2011;17:1–6.
45. Ibarra P, Arango J, Bayter J, Castro J, Cortes J, Lascano M, Lema-Flores E.Consenso de la Sociedad Colombiana de Anestesiología y Reanimación, SCARE, y de la Sociedad Colombiana de Cirugía Plástica sobre las recomendaciones para manejo de pacientes electivos de bajo riesgo. Rev Col Anest. 2009;37(4):390–403.
46. Guerrerosantos J, Gonzalez-Mendoza A, Mesmela Y, Gonzalez M, Deos M, Diaz P. Long­term survival of free fat grafts in muscle: an experimental study in rats. Aesthetic Plast Surg. 1996;20:403–8.
47. Cardenas-Camarena L, Arenas-Quintana R, Robles-Cervantes J. Buttocks fat grafting: 14 years of evolution and experience. Plast Reconstr Surg. 2011;128:545–55.
48. Rapkiewicz A, Kenerson K, Hutchins K, Garavan F, Lew E, Shuman M.Fatal complications of aesthetic techniques: the gluteal region. J Forensic Sci. 2018;1:1–7.
49. Mod M, Teitelbaum S, Suissa D, Ramirez-Montañana A, Astarita D, Mendieta C, Singer R.Report on mortality from gluteal fat grafting: recommendation from the ASERF task force. Aesthet Surg J. 2017;1:1–11.
50. Ramos-Gallardo G, Orozco-Renteria D, Medina-Zamora P, Mota-Fonseca E, Garcia­Benavides L, Cuenca-Pardo J, Contreras-Bulnes L.Prevention of fat embolism in fat injection for gluteal augmentation, anatomic study in fresh cadavers. J Invest Surg. 2017;31(4):292–7.
51. Ramos-Gallardo G, Medina-Zamora P, Cardenas-Camarena L, Orozco-Renteria D, Duran­Vega H, Mota-Fonseca E. Where does the transplanted fat is located in the gluteal region? Research letter. J Invest Surg. 2019;32(3):228–31.
52. Villanueva N, Del Vecchio D, Afrooz P, Carboy J, Rohrich R.Staying safe during gluteal fat transplantation. Plast Reconstr Surg. 2018;141:79–86.
53. Ghavami A, Villanueva N, Amirlak B.Gluteal ligamentous anatomy and its implication in safe buttock augmentation. Plast Reconstr Surg. 2018;142:363–71.
54. Abboud M, Dibbo S, Abboud N. Power-assisted gluteal augmentation: a new technique for sculpting, harvesting, and transferring fat. Aesthet Surg J. 2015;35(8):987–94.
55. Everett M, Morales R Jr, Newall G, Fortes P, Hustak K, Patronella C, etal. Safest practices for autologous buttock augmentation with fat grafting using a roller pump injection technique. Aesthet Surg J. 2018;38(7):751–62.
56. Del Vecchio D, Wall S Jr. Expansion vibration lipolling: a new technique in large-volume fat transplantation. Plast Reconstr Surg. 2018;141:639–49.
57. Aslani A, Del Vecchio D.Composite buttock augmentation: the next frontier in gluteal aes­thetic surgery. Plast Reconstr Surg. 2019;144(6):1312–21.
Part III
Fat Science
Chapter 10
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The Science Behind theFat Graft
GabrielA.Mecott andSheylaGeraldineHernández-Soto
10.1 Introduction
Fat grafting is one of the most useful tools for the plastic surgeon. It can be used to improve contour, ll defects, and improve the appearance and quality of the skin, among other uses. In aesthetic surgery, is used to modify the shape and volume of the desired areas, such as buttocks, breast [1, 2], chest, and abdominal muscles [3]. The fat has been described as having most of the characteristics of the ideal ller, due to its biocompatibility, versatility, and stability, as well for being long-lasting, natural-appearing [4] and amenable to be harvested repeatedly [5]. However, it is not exempt of complications or drawbacks: loss of volume, fat necrosis, and infec­tion are some of the most common problems of fat grafting.
The loss of volume is evidently the most frustrating problem faced with fat graft­ing. The rst report of loss of volume of the transplanted fat was in 1950, when Peer reported the loss of 45% of the grafted fat [6]. Nonetheless, the reported loss in volume after fat grafting usually ranges from 20 to 90% [713]. Plastic surgeons are still struggling to achieve predictable outcomes and high take rates of fat grafts [14]. As stated by Illouz, adipocytes are highly fragile cells with very short lives that once removed from the body, cannot tolerate excessive manipulation, refrigeration, or major trauma associated with harvesting [15]. Now, we know that adipocytes are the most fragile cells among all the cells contained in the grafted fat and they die easily under ischemic conditions [16].
The rst documented fat autograft was in 1893 by Neuber, in the form of frag­ments of 1cm obtained from the arm of one patient [17], followed by Lexer [18]
G. A. Mecott (*) · S. G. Hernández-Soto Plastic and Reconstructive Surgery Division, Faculty of Medicine and University Hospital, Universidad Autónoma de Nuevo León, Monterrey, Mexico e-mail: drmecott@nubody.com.mx
© Springer Nature Switzerland AG 2023 D. Del Vecchio, H. Durán (eds.), Aesthetic Surgery of the Buttock,
https://doi.org/10.1007/978-3-031-13802-7_10
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146
G. A. Mecott and S. G. Hernández-Soto
and Brunings [19]. Most of the initial reports of fat grafting were in the form of dermofat grafts [6, 2023]. In 1955, Peer stated, in his cell survival theory, that the number of viable cells grafted correlates with the ultimate volume of graft survival [24]. However, the initial techniques of fat harvesting and lack of proper technology for decreasing cell damage during processing, make difcult to improve viability of the adipocytes.
Due to the technical difculties and unpredictable results, fat grafting was aban­doned for several decades. Renewed interest in autologous fat grafting started in the late 1970s [25]. With the description of the liposuction by Arpad and Giorgio Fisher in 1975 [26], the possibility of obtaining fat without large incisions was created. However, it was until 1977 that liposuction was described as a procedure intended for grafting purposes [27].
In 1993, Lewis published an interesting article about the frustration that many surgeons faced those days and how many of them abandoned fat grafting. This arti­cle reects the skepticism that prevailed at that time about the utility of fat grafting for long-term results. He nished his article by suggesting that for those surgeons who say fat grafting does not work, they soul better say “in my hands” it does not work [28]. It is amazing how nowadays this advice might still be valid.
Two years later, Coleman published his rst article about fat grafting, trying to demonstrate the long-term effect of fat grafting as a ller [29]. In this article he proposed three principles to keep in mind when performing fat grafting:
• Fat is a delicate structure easily damaged by mechanical and chemical insults.
• True injected volume is difcult to judge if too much oil, blood, or lidocaine is
injected.
• Fat is a living tissue that should be placed next to viable tissue that provides
adequate nutritional and respiratory source to survive.
He also advised to not to inject too much fat, because the fat could migrate due to the excess of pressure and would have necrosis if the graft is not placed next to viable tissues. Thus, he suggested to perform multiple surgeries instead of risking for migration or fat necrosis. These recommendations are still valid as of today. We know that grafting is the process of placing some tissue deprived from its blood sup­ply (in this case adipocytes), in a receptor area that will provide nutrients to the grafted cells. Then, the whole process of fat grafting should focus on placing viable adipocytes in the receptor area in a way that these cells receive adequate blood sup­ply in order to assure the survival of most grafted cells.
In recent years, the cell replacement theory was described. This theory proposes that most adipocytes will die due to ischemia, and will be replaced by new adipo­cytes during the rst 3months after grafting [30]. Nevertheless, both theories (i.e., survival and replacement theories) are not mutually exclusive, since viable preadi­pocytes should also be injected and placed in viable tissue in order to survive.
The process of fat grafting, as in skin grafts, relies on different variables such as donor-site election, harvesting, processing, and injection techniques [31]. Each step involves several variables that might inuence the survival of the graft and will be discussed in this chapter.
10 The Science Behind theFat Graft
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10.2 Fat Integration andRemodeling
The process of how the adipocytes survive after they are grafted is not well under­stood. It is evident that there is some bleeding after the fatty graft when it is placed into the recipient site, initiating release of multiple cytokines and pro inammatory mediators. This might create an inammatory environment. Then, the adipocytes will be under ischemic conditions and would survive by nourishing from the vascu­larized surrounding tissues, by diffusion, until direct capillary vessels are formed [32].
In the rst few weeks after grafting, the volume of the grafts increases slightly as swelling is observed. Over the next 6weeks, the volume returns to baseline and stabilize at week 8 [33]. The nal loss of the volume of the fat grafting is the result of necrosis and ultimately brosis and removal of the death tissue.
In the late 1960s, Sawhney etal. described the behavior of a dermal-fat graft in porcine models. They grafted pieces of 1.5 cm (per side) between the anterior abdominal rectus sheath and the rectus muscle. They found that the mass of the fat decreased in size about 60% by the second week, 80% by the fourth week, and was almost absent by the eighth week. Most of the fat was replaced with brotic tissue [21]. It is important to note that they placed the grafts with the dermal side in contact with the muscle and the fatty portion in contact with the rectus sheath and this might be the reason for the loss of most of the adipocytes. However, this model explains in detail the fate of the fat graft when is not well vascularized.
Coban and Ciralik clamped an epigastric ap (rodents) up to 4h and found that fat necrosis was found at all timepoints, but maximum signs of necrosis were seen after 3h of ischemia. They also stated that the necrotic volume of fat did not changed after 3h [34]. Thus, this study implies that 3h might be the maximum time of isch­emia that the adipocyte can recover from.
Kato and cols created a model of fat grafting trying to describe the behavior of the fat graft invivo. In their model, they found that the adipocytes formed three dened zones after grafting [30] (Fig.10.1).
1. The rst is the surviving (supercial) zone. (100–300μm thick), demarcated at
the end of the rst week. This is the area with viable adipocytes and adjacent to a vascularized bed.
2. The second zone is the regenerating (intermediate) zone. (600–1200μm thick)
and is seen between the third and fourth week after grafting. In this zone, the grafted adipocytes die within the rst 24h, but Adipose Stem Cells (ASCs) sur­vive and promote angiogenesis for up to 3days. During the next 3months, new adipocytes replace the death ones and the tissue is repaired.
3. The nal is the necrosis (central) zone. Here, the adipocytes also die within the
rst 24h, but the remaining ASCs cannot promote revascularization and all cells nally die by the third day. The result is cicatrization or oil cyst formation.
As mentioned previously, the adipocytes that don’t receive blood supply and nutrients die within the rst 24h, and they are surrounded by macrophages. This process causes their volume to be retained for about 4weeks after their death [35].