B. Amylase

C. Lactate dehydrogenase

D. Aldolase

E. Creatin kinase

96. Researchers isolated 5 isoenzymic forms of lactate dehydrogenase from the human blood serum and studied their properties. What property indicates that the isoenzymic forms were isolated from the same enzyme?

A. Catalyzation of the same reaction

B. The same molecular weight

C. The same physicochemical properties

D. Tissue localization

E. The same electrophoretic mobility

97. A patient presents high activity of LDH1,2, aspartate aminotransferase, creatine phosphokinase. In what organ (organs) is the development of a pathological process the most probable?

A. In the heart muscle (initial stage of myocardium infarction)

B. In skeletal muscles (dystrophy, atrophy)

C. In kidneys and adrenals

D. In connective tissue

E. In liver and kidneys

98. Marked increase of activity of МВ-forms of CPK (creatinephosphokinase) and LDH-1 were revealed on the examination of the patient's blood. What is the most likely pathology?

A. Miocardial infarction

B. Hepatitis

C. Rheumatism

D. Pancreatitis

E. Cholecystitis

99. 12 hours after an accute attack of retrosternal pain a patient presented an increase of aspartate aminotransferase activity in blood serum. What pathology is this deviation typical for?

A. Myocardium infarction

B. Viral hepatitis

C. Collagenosis

D. Diabetes mellitus

E. Diabetes insipidus

100. Desulfiram is widely used in medical practice to prevent alcocholism. It inhibits aldehyde dehydrogenase. Increased level of what metabolite causes aversion to alcochol?

A. Acetaldehyde

B. Ethanol

C. Malonyl aldehyde

D. Propionic aldehyde

E. Methanol

101. A 49-year-old driver complains about unbearable constricting pain behind the breastbone irradiating to the neck. The pain arose 2 hours ago. Objectively: the patient’s condition is grave, he is pale, heart tones are decreased. Laboratory studies revealed high activity of creatine kinase and LDH1. What disease are these symptoms typical for?

A. Acute myocardial infarction

B. Acute pancreatitis

C. Stenocardia

D. Cholelithiasis

E. Diabetes mellitus

102. In case of enterobiasis acrihine – the structural analogue of vitamin B₂ - is administered. The synthesis disorder of which enzymes does this medicine cause in microorganisms?

A.FAD-dependent dehydrogenases

B. Cytochromeoxidases

C. Peptidases

D. NAD-dependet dehydrogenases

E. Aminotransferases

103. Hydroxylation of endogenous substrates and xenobiotics requires a donor of protons. Which of the following vitamins can play this role?

A. Vitamin C

B. Vitamin P

C. Vitamin B₆

D. Vitamin E

E. Vitamin A

104. A newborn child has convulsions that have been observed after prescription of vitamin B₆. This most probable cause of this effect is that vitamin B₆ is a component of the following enzyme:

A. Glutamate decarboxylase

B. Pyruvate dehydrostase

C. Netoglubarate dehydromine

D. Aminolevulinate synthase

E. Glycogen phosphorylase

105. In a patient with complaints on pain in cardiac area a myocardial infarction was recognized after estimation of enzymes activity in blood. Indicate, please, what enzyme activities were determined?

1. LDH, creatine kinase, aminotransferase

2. Amylase, lipase, phosphatase

3. Peptidase, arginase, glucokinase

4. Trypsin, lysozyme, citrate synthase

5. Aldolase, succinate dehydrogenase, hexokinase

106. In course of tuberculosis treatement a patient was administered isoniazide – a structural analogue of nicotinamide and pyridoxine. What type of inhibition by mechanism of action exhibits isoniazide?

1. Competitive

2. Irreversible

3. Uncompetitive

4. Allosteric

5. Noncompetitive

107. After laboratory investigation in blood of patient an increase of LDH activity was detected, which is characteristic symptom of heart, liver or kidney diseases. What additional biochemical investigation must be performed in differential diagnostics?

1. Determination of LDH isozymes

2. Estimation of blood glucose level

3. Ketone bodies level in blood

4. Determination of blood cholesterol level

5. Amylase activity in blood

108. In a patient a preliminary diagnosis of myocardial infarction was proposed. A characteristic feature of this disease is a marked increase in blood in the first 24 hours of the next enzyme:

1. Creatine-phosphokinase

2. Arginase

3. Catalase

4. Glc-6-P dehydrogenase

5. Alpha-amylase

109. Ambulance delivered a patient to the hospital with a preliminary diagnosis “acute pancreatitis”. What enzyme activity must be estimated in blood and urine in order to support this diagnosis?

1. Alpha-amylase

2. AlAT (GPT)

3. AsAT (GOT)

4. Gamma-amylase

5. Lactate dehydrogenase

110. In dietology, in cases of children milk intolerance a milk is used in which lactose content is diminished enzymatically. What enzyme is used for this purpose?

1. -galactosidase

2. –glucosidasde

3. –amylase

4. –glucosidase

5. –amylase

111. In cases of chronic pancreatitis a decrease of trypsin production and secretion is observed. Digestion and absorption of what substances is injured in this case?

1. Cleavage of proteins

2. Cleavage of polysaccharides

3. Cleavage of nucleic acids

4. Cleavage of disaccharides

5. Cleavage of lipids

112. After the addition of an extract of pancreatic gland to the tube with starch solution, a blue coloration of the sample with iodine have disappeared, which indicates on starch hydrolysis. What pancreatic enzyme is involved in this reaction?

1. amylase

2. Chymotrypsin

3. Lipase

4. Aldolase

5. Trypsin

113 During the investigation of pancreatic juice was detected a great number of enzymes. Some of them are secreted in inactive form. What are these enzymes?

1. Trypsinogen, chymotrypsinogen

2. Sucrase, amylase

3. Ribonuclease, pepsin

4. Amylase, lipase

5. DNA-ase, aminopeptidase

114. Phosphororganic compound diisopropyl-fluorophosphate is a dangerous toxin as it inhibits cholinesterase. What is the mechanism of this inhibition?

1. Irreversible

2. Reversible

3. Competitive

4. Uncomoetitive

5. Noncompetitive

115. In blood serum of a patient a marked increase in activity of trypsine, alpha-amylase and lipase was detected. What disease can be suggested?

1. Acute pancreatitis

2. Cholelythiasis

3. Chronic hepatitis

4. Malignant tumors

5. Poisoning with insecticides

116. In a patient a preliminary diagnosis of myocardial infarction was proposed. A characteristic feature of this disease is a marked increase in blood in the first 24 hours of the next enzyme:

1. Creatine kinase

2. Arginase

3. Catalase

4. Glc-6-P dehydrogenase

5. Alpha-amylase

117. During the surgery after injection of a drug, which cause the myorelaxation, in patient occures a prolonged stoppage of resptration (more then 5 min). What enzyme insufficiency may be responsible for this accident?

1. Acetylcholine esterase

2. Catalase

3. Glucose-6-phosphate dehydrogenase

4. Monoaminoxidase

5. Acetyl-transferase

118. In blood serum of a patient a marked increase in activity of trypsine, alpha-amylase and lipase was detected. What disease can be suggested?

1. Acute pancreatitis

2. Cholestasis

3. Chronic hepatitis

4. Malignant tumors

5. Insecticide poisoning

119. In blood serum of a patient a marked increase of activity of creatine phosphokinase and lactate dehydrogenase was detected. . What disease can be suggested as a cause?

1. Myocardial infarction

2. Acute pancreatitis

3. Chronic pancreatitis

4. Hemolytic jaundice

5. Nephrosis

120. In blood serum of a patient a high activity of isozyme LDH-1 was detected. In what organ pathological changes occure?

1. Heart

2. Liver

3. Sceletal muscles

4. Pancreas

5. Kidney

121. During investigation of gastric secretory function was detected decrease of hydrochloric acid content in gastric juice. What enzyme activity will decrease in this case?

1. Pepsin

2. Lipase

3. Hexokinase

4. Amylase

5. Carboxypeptidase

122. In malignant tumor of prostata a marked increase in activity of the next enzyme in blood serum is observed:

1. Acid phosphatase

2. Aldolase

3. Alanyl aminotransferase

4. Lactate dehydrogenase

5. Alkaline phosphatase

123. On the third day following onset of acute myocardial infarction, which enzyme estimation will have the best predictive value?

1. Serum LDH

2. Serum AST

3. Serum CK

4. Serum ALT

5. Serum amylase

124. What enzyme may be used in substitute therapy in cases of gastric secretion insufficiency?

1. Pepsin

2. Trypsin

3. Hyaluronidase

4. Collagenase

5. Ribonuclease

125. In a patient the disorder of proteins digestion in stomach and small intestine is observed. What enzymes insufficiency cause this disorder?