Основы биологии_Лекция-5_Макеев (2013)
.pdf&!' : # % 3D 1D
/$
&!'
$- /-
$%( $ ( $ ( 100
+ $ ( $
Wasserman, W.W. and Sandelin, A. (2004)
, 5, 2010 |
|
5 ( ( $% # " &!'-
(
4 % $ &!'
$% /-# % (SELEX)
% ( (Protein binding assays, PBA) 2 + (EMSA, gel shift)
$ &!' I
$# # % (ChIP-, -chip, -seq, -pet, -exo…)
, 5, 2010 |
|
! , %-. + 4
- 1 . .
|
In vivo/ |
%/ |
& |
|
! |
|
in vitro |
- |
|
( |
' |
|
% &!' |
|
|
||
|
|
|
|||
|
|
|
|
|
|
ChIP |
In vivo |
% |
500 - |
500 – |
! % |
{Seq, Pet, Chip} |
|
5000 |
50 000 |
% ( |
|
|
|
(Piggy back) |
|||
|
|
|
|||
|
|
|
|
|
|
|
|
|
|
|
|
% |
in vivo |
. |
28 |
20-50 |
% ( |
|
(( |
|
|
% |
|
|
() |
|
|
|
|
|
|
|
|
|
|
SELEX, RSS |
In vitro |
. |
20 |
20-50 |
$ $ |
|
|
|
|
|
|
|
|
|
|
|
|
PBA |
In vitro |
. |
60 |
>10000 |
|
|
|
|
|
|
|
$( |
In vitro |
% |
20-100 |
5-30 |
|
|
|
|
|
. . |
|
|
|
|
|
|
|
|
|
|
|
|
|
& % ( , $( % /
% 4, ( 0 (
, 5, 2010 |
|
4 &!'
1 &!', % (-.
$% ( +
+ ( &!'
CCCGACCA
AAATTTAT
(piggy back)
CCCGACTA
! ( $ &!',
/ (
( % (-. (
4, + + . 4
-% $ &!',
% (-. / 4.
Wasserman, W.W. and Sandelin, A. (2004)
, 5, 2010 |
|
+ ( 4?
…AGACTTCCACGAACTTTA…
+ ( &!' (rSNP, remodeling)
+ (
$% (
Suzuki, Forrest (2009)
Nature genetics
, 5, 2010 |
|
$# # % (ChIP)
# % ChIP-chip
ChIP-seq
2 ( ChIP-PET
/$ ChIP-exo
: http://www.tigr.org/
&: ( % ( % in vivo
!: $% % ( "
~300 , - ChIP-exo
, 5, 2010 |
|
* (
BLAST Basic Local Alignment Search Tool blast.ncbi.nlm.nih.gov
nucleotide blast
Search a nucleotide database using a nucleotide query protein blast
Search protein database using a protein query blastx
Search protein database using a translated nucleotide query tblastn
Search translated nucleotide database using a protein query tblastx
Search translated nucleotide database using a translated nucleotide query
, 5, 2010 |
|