Лекция_4_Б
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Rice Tungro |
Maize Ubi 1 |
Rice Actin 1 |
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Virus |
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Leaf |
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elongation of |
zone and tip – Root |
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Inflorescence |
Immature |
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flower Immatuer |
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pollen and |
filament Anther, |
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seed Mature |
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Embryo |
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Mapping expression of ‘broadly constitutive’, tissue-specific and inducible promoters
Rice Tungro Bacilliform Virus – no expression in roots, or pollen
Barley heat-shock promoter (Hvhsp17)::GUS
Will allow transformation of ‘lethal genes’
Whole plant or pseudo-tissue-specificity
Same organ from transformed plant |
Developing seeds (dpa) from transformed plant (all heat shocked) |
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23 |
26 |
28 |
40°C water bath for 1hr
no |
+ 40°C |
heat shock |
heat shock |
J. Freeman, C.A. Sparks, J. West, P. R. Shewry & H.D. Jones (2011) Temporal and spatial control of transgene expression using a heat-inducible promoter in transgenic wheat. On Line Early: Plant Biotechnology Journal
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rbcS |
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rbcS |
Lch |
cry3aM |
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LicB |
polyA |
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rbcS Lsc cry3aM LicB polyA
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Targeting proteins using transit peptides
GFP guard cell chloroplasts fluorescence + light image
maize ferredoxin III
amyloplasts in wheat endosperm presequence targeting
GFP to Plastids
amyloplasts in wheat pollen
Histone 2B transit peptide: GFP
Freeman Huttly Kurup et al. unpublished
GFP targeted to wheat leaf nuclei
LF Primavesi, H Wu, EA Mudd, A Day and HD Jones (2008) Visualisation of plastids in endosperm, pollen and roots of wheat expressing modified GFP fused to transit peptides from wheat SSU RubisCO, rice FtsZ and maize ferredoxin III proteins .
Transgenic Research 17(4): 529-543
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CN
CK1
CB
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120 |
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, |
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100 |
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80 |
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60
40
20
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rbcS licB)2
35S CaMV 
LS
licB)2
rbcS 
LS licB)2
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12 24 |
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100
80
60
40
20
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CN |
CB |
CK1 |
*! |
Ptrc licBM2 |
Kmr |
*! |
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p6803-licB/pilA |
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p6803-licB/2016
p6803-licB/art (+)
p6803-licB/art (-)
p6803-licB/control
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WT |
licBM2/control licBM2/pilA licBM2/2016 licBM2/art (+) licBM2/art (-) |
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