Лекция_4_Б
.pdf
7 /
Inducer
Inducible transcription factor |
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Protein of interest |
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35S PRO |
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iTF |
Ter |
Ind. PRO |
goi |
Ter |
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nucleus
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7 |
7 |
7 |
90 |
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80 |
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70 |
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60 |
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50 |
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% |
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40 |
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30 |
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20 |
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10 |
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0 |
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' |
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! |
rbsc "
35S & CaMV "
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100 |
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5 |
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% |
4,5 |
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4 |
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" # $, |
3,5 |
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3 |
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2,5 |
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2 |
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1,5 |
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1 |
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0,22 |
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0,25 |
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0,5 |
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0 |
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0 |
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TDH |
TDH- |
LTRforw- |
LTRrev- |
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licBM2 |
licBM2 |
licBM2 |
( "
# "
rbcS
35S
5
7
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7
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5 |
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,
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$
&'#9#2 5%7$+0 9 7$ #$2': +0
.&927$ 2 +0 # .%'#927$ 2 +0 )2$%-% $27$#5% & #; &.$# %7$#
%$7 $7$ #2 "% % %8 &.$# %7$#
%$7 $7 #2 3 &9#$2': %(% '#; #; & )2$&,%'#3) 0%3;# &
2,%':1%8 5&3)25
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52 %5$25 |
"% % &; |
7'#; #2 & |
7#7$2)+ |
IN SITU, IN |
5%922 |
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&.$# - |
N-.% 62 |
& &'#3& |
VIVO |
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%7$: |
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CAT |
- |
- |
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- |
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LUX |
- |
- |
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IN SITU |
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, |
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LACZ |
+ |
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IN SITU |
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NPTII |
- |
- |
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- |
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GUS |
+ |
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IN SITU |
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- |
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, |
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GFP |
+ |
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IN SITU, |
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IN VIVO |
/ |
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" # "
) #
-) ! #
# !