Лекция_4_Б
.pdf
$--. --
, ,
,
.
3
$$---- ..??
.
-
|
|
|
|
|
|
|
|
,, |
|
|
|
|
|
$$---- .. |
|
|
** |
|
|
|
|
|
|
|
|
.. |
|
|
$ |
|
|
|
|
|
|
|
|
" ",", |
|
|
!! 2525 |
|
|
.. 44 |
|
|
,, -- |
|
|
,, ** |
- |
$$---- .... |
|
|
||
tgaccccccaggatatattggcgct |
tggcaggatatattgtggtgtaaac |
|
, ,,
,
$---- ..
$--.,, ""
""
. |
4 |
|
|
|
$$---- .. |
|
|
|
|
|
|
,, |
|
||
,,
*!*!
. $ ,,
,,
, //
*!*!
$$---- ..
- ., //
.
$ ,
. # ,,
/ ( ) ..
%
%
( )
1982
SUGARS |
|
|
|
||
ChvE |
VirA |
PHENOLICS |
|
||
|
|
||||
1) CHEMO-SENSING |
|
|
|
|
|
|
VirG |
|
|
|
|
|
2) vir INDUCTION |
7) CYTOPLASMIC |
|||
|
TRAFFICKING |
||||
|
3) T-DNA PROCESSING |
||||
vir GENES |
|
||||
4) T-DNA & VIR |
8) NUCLEAR |
||||
|
|||||
|
PROTEIN EXPORT |
TARGETING |
|||
|
|
|
Type IV |
|
|
Ti-PLASMID |
|
|
Secretion |
T-COMPLEX |
|
|
T-STRAND |
system |
|||
T-DNA |
|
||||
|
|
|
|
||
VirE1 |
VirD2 |
Nuclear |
|||
|
|
pore |
|||
|
|
|
|
||
|
|
|
|
complex |
|
VirD5 VirE2 |
|
5) ATTACHMENT |
|
||
VirD1/VirD2 |
VirF |
|
6) T-STRAND AND VIR |
||
PROTEIN TRANSLOCATION |
|||||
|
|||||
|
VirE3 |
||||
|
|
|
|
||
NUCLEUS
9)VIR PROTEIN REMOVAL
10)T-DNA INTEGRATION
11) TRANSGENE
EXPRESSION
Integrated
T-DNA
Agrobacterium tumefaciens |
PLANT CELL |
4
-
|
|
|
5 / 6 5 / |
||||||
|
|
|
5 / 6 5 / |
||||||
|
|
|
|
|
|||||
|
|
( |
|
||||||
|
|
( |
|
|
|
|
|||
|
|
|
|
|
|
||||
|
|
||||||||
|
|
|
|
||||||
|
|
|
|
|
|
|
|||
|
|
|
|
|
|
||||
|
|
|
|
|
|
|
|||
|
|
|
|
|
|
|
|
||
|
|
|
|
|
|
|
|
|
|
TL35S: |
RB |
Pnos |
nptII |
pAg7 |
P35S |
proBosm |
pA |
LB |
|
-
tgaccccccaggatatattggcgct |
tggcaggatatattgtggtgtaaac |
|