Лекция_5_ГИ_Б
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$ / % |
, . . |
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DesA |
, %-12 |
949 |
949 |
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DesC |
, %-9 |
777 |
777 |
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LicB |
, |
# |
632, |
369, |
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β-1,3-1,4- ( $ ) |
291 |
291 |
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nptII |
, |
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473 |
473 |
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" " " |
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NtGA2 |
, α– |
609 |
- |
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GTP- ! |
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Act |
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500 |
600 |
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virE |
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A. |
tumefaciens |
600 |
600 |
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AGLO |
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P-35S |
35S CaMV |
382 |
382 |
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. .
1500
1000
700
500
400
200
M desA desC LicB nptII T-nos VirE |
act NtGA2 M |
т
р
а
н
с
фdesC
о777 п.о.
р
м
а
н
т
к
о
н
т
р
о
л
ь
licB act
v T1 T2 T3 K
! "#$
: licB (632 . .) act (500 . .). v – % , T1-T3 –
& ', $ – .
nptII
. .
1500
1000
700
500
300
M v K T1 T2 w v K T1 T2 w |
v K T1 T2 |
( – , v – % ; T1, T2 –
& '; $ –
; w – ( "#$
').
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LB |
P-nos |
nptII |
T-nos |
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P-35S |
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LicB |
T-ocs |
RB |
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ter |
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bar |
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$ / % |
, . . |
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DesA |
, %-12 |
949 |
949 |
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DesC |
, %-9 |
777 |
777 |
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LicB |
, # |
632, |
382, |
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β-1,3-1,4- ( $ ) |
291 |
291 |
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NtGA |
, α– |
609 |
- |
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GTP- ! |
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Act |
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500 |
600 |
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virE |
A. tumefaciens |
600 |
600 |
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AGLO |
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T-nos |
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188 |
188 |
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P-35S |
35S CaMV |
382 |
382 |
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1500 |
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1000 |
desA |
700 |
Act |
500 |
nptII |
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300 |
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200 |
T-nos |
( v P1 K P1 P2 w v P2 K P1 P2 w
! "#$ '
: DesA (949 . .), Act (500 . .), nptII (473 . .) T-nos (188 . .). ( –
, w – ( "#$
'), v – % , P1,P2 – &
', $ – .
act
licB
Tnos
'
+ – "#$ AGLO, – ; &2' – ( "#$ ).
desA-licB
desA
virE licB act
T-nos
desA
LicB
act
T-nos
( w v T1 T2 T3 T4 T5 T6 T7 T8 T9 K M
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( – , |
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w – ( |
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"#$ '), |
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v – % , |
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T1-T12 – |
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& ', |
М v |
– . |
Т10 Т11 Т12 w |