Ординатура / Хирургия / Библиотека им академика М.И. Перельмана / Книга_5936_Библиотеки_им_академика_М_И_Перельмана

The remodelling of induced cardiomyocytes (iCMs) is of specific importance in re­generative medicine. It, however, remains a major challenge to assemble a safe and effective process of delivering gene and to effect the remodelling of iCMs for therapeu­tic implementation in heart trauma. Yang et al. [18] reported branched polyethylenei­mine (BP)-coated nitrogen-enriched CDs (BP-NCDs) as effective nanocarriers filled with microRNAs-combo (BP-NCDs/MC) for cardiac remodelling. They prepared and charac­terized the BP-NCD nanocarriers using different analytical techniques. From their re­sults, the BP-NCD nanocarriers indicated minor cytotoxicity, long-term microRNAs impression, and good microRNA-combo binding affinity. Another important observa­tion is leading BP-NCDs/MC nano-complexes to effective direct r emodelling of fibro­blasts into iCMs in the absence of genomic integration and bringing about efficient cardiac function recovery following myocardial infarction. Their study offered a new approach in providing efficient and safe microRNA-delivery nano-platforms predicated on CDs for favourable disease therapy and cardiac regeneration. In another research, Wang et al. [19] developed tetraethylene pentamine CDs (TEPA-CDs) as nanocarriers of siRNA based on TEPA and glucose using bottom-up method. They prepared the CDs by one-step oil bath heating. The synthesized TEPA-CDs have a zeta potential of 19.17 mV, an average diameter of 8.6 nm and with positively charged decoration. Their result re­veals that TEPA-CDs could precipitate siRNA into firm complexes without detectable premature release. Based on cellular uptake analysis, the Cy3-labelled siRNA was taken up by HeLa cells. Their study showed that TEPA-CDs could be useful as siRNA nanocar­rier to tumour cells.

In this chapter, the applications of CDs in protein and nucleic acid delivery into ani­mal, plants, and bacteria cells were reviewed. CDs were found to be excellent in deliv­ering siRNA, DNA, and other gene cargos into different targeted cell lines. The CDs are known to exhibit excellent biocompatibility stable fluorescence, high quantum yield, and negligible toxicity. The interesting properties of CDs make them safe and efficient nucleic acid and protein nanocarriers. The potent fluorescence and good stability of CDs indicate that the delivery of protein and nucleic acid efficiency could be deter­mined easily by fluorescence, which exhibits a great capacity as delivery agent with distinctive property.